Easily Controlled Grafting of Oligonucleotides on gamma Fe sub(2)O sub(3) Nanoparticles: Physicochemical Characterization of DNA Organization and Biological Activity Studies

We report a one-step process to functionalize superparamagnetic iron oxide nanoparticle (SPIO-NP) surfaces with a controlled number of oligonucleotides. For this study, we use a specific oligonucleotide targeting the signal transducer and activator of transcription 3 (STAT3), a key regulator of cell...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:The journal of physical chemistry. B 2014-02, Vol.118 (6), p.1535-1544-1535-1544
Hauptverfasser: Geinguenaud, Frederic, Souissi, Ines, Fagard, Remi, Lalatonne, Yoann, Motte, Laurence
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 1544-1535-1544
container_issue 6
container_start_page 1535
container_title The journal of physical chemistry. B
container_volume 118
creator Geinguenaud, Frederic
Souissi, Ines
Fagard, Remi
Lalatonne, Yoann
Motte, Laurence
description We report a one-step process to functionalize superparamagnetic iron oxide nanoparticle (SPIO-NP) surfaces with a controlled number of oligonucleotides. For this study, we use a specific oligonucleotide targeting the signal transducer and activator of transcription 3 (STAT3), a key regulator of cell survival and proliferation. This oligonucleotide is self-complementary and can adopt a hairpin structure. It is labeled with the fluorescein amidite group at the 3'-end. The polyanionic DNA is electrostatically attracted onto the positively charged surface of the bare SPIO-NPs. During synthesis, the molar ratio between the oligonucleotides and nanoparticles was varied from 17.5 to 175. For particles with a mean diameter of 10 nm, a nanoparticle surface saturation is observed corresponding to 70 DNA strands per particle. The increase of DNA density per nanoparticle is correlated to a transition from the hairpin structure adsorbed horizontally on the nanoparticle surface to a vertically ordered surface packing assembly. An in vitro study on human colon carcinoma cell line SW480 shows that the kinetics of internalization and biological activity of the NPs seem to be dependent on the oligonucleotide density. Cell death and the kinetics of internalization are favored by a low density of oligonucleotides.
doi_str_mv 10.1021/jp410899a
format Article
fullrecord <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_miscellaneous_1753529863</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1753529863</sourcerecordid><originalsourceid>FETCH-LOGICAL-p663-ed60cc86fc173cb9c1a81b51e33188343d9101ccf304350bef9f77a0cba54f013</originalsourceid><addsrcrecordid>eNqFjLFOwzAYhCMEEqUw8AYe2yFgx3HisJXSFqSqQaJ79cexU1dOHGIHqbwT70hUYGY43el09wXBLcF3BEfk_tDGBPMsg7NgRFiEw0Hp-W9OCE4ugyvnDhhHLOLJKPhagNPmiOa28Z01RpZo1YHyuqmQVSg3urJNL4y0XpfSIdugCuoa0FIi1xeTaJqfnE7RBhrbQuf1sHYP6HV_dFpYsZe1FmDQfA8dCC87_QleD5wB_7SZobyroPnroCnRo7bGVqfPTHj9of0Rvfm-1NJdBxcKjJM3vz4OtsvFdv4crvPVy3y2DtskoaEsEywET5QgKRVFJghwUjAiKSWc05iWGcFECEVxTBkupMpUmgIWBbBYYULHweQH23b2vZfO72rthDQGGml7tyMpoyzKeEL_nzIac0IZTek3ToCBGw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1534813537</pqid></control><display><type>article</type><title>Easily Controlled Grafting of Oligonucleotides on gamma Fe sub(2)O sub(3) Nanoparticles: Physicochemical Characterization of DNA Organization and Biological Activity Studies</title><source>American Chemical Society Journals</source><creator>Geinguenaud, Frederic ; Souissi, Ines ; Fagard, Remi ; Lalatonne, Yoann ; Motte, Laurence</creator><creatorcontrib>Geinguenaud, Frederic ; Souissi, Ines ; Fagard, Remi ; Lalatonne, Yoann ; Motte, Laurence</creatorcontrib><description>We report a one-step process to functionalize superparamagnetic iron oxide nanoparticle (SPIO-NP) surfaces with a controlled number of oligonucleotides. For this study, we use a specific oligonucleotide targeting the signal transducer and activator of transcription 3 (STAT3), a key regulator of cell survival and proliferation. This oligonucleotide is self-complementary and can adopt a hairpin structure. It is labeled with the fluorescein amidite group at the 3'-end. The polyanionic DNA is electrostatically attracted onto the positively charged surface of the bare SPIO-NPs. During synthesis, the molar ratio between the oligonucleotides and nanoparticles was varied from 17.5 to 175. For particles with a mean diameter of 10 nm, a nanoparticle surface saturation is observed corresponding to 70 DNA strands per particle. The increase of DNA density per nanoparticle is correlated to a transition from the hairpin structure adsorbed horizontally on the nanoparticle surface to a vertically ordered surface packing assembly. An in vitro study on human colon carcinoma cell line SW480 shows that the kinetics of internalization and biological activity of the NPs seem to be dependent on the oligonucleotide density. Cell death and the kinetics of internalization are favored by a low density of oligonucleotides.</description><identifier>ISSN: 1520-6106</identifier><identifier>EISSN: 1520-5207</identifier><identifier>DOI: 10.1021/jp410899a</identifier><language>eng</language><subject>Biological ; Colon ; Density ; Deoxyribonucleic acid ; Nanoparticles ; Oligonucleotides ; Surface chemistry ; Transducers</subject><ispartof>The journal of physical chemistry. B, 2014-02, Vol.118 (6), p.1535-1544-1535-1544</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Geinguenaud, Frederic</creatorcontrib><creatorcontrib>Souissi, Ines</creatorcontrib><creatorcontrib>Fagard, Remi</creatorcontrib><creatorcontrib>Lalatonne, Yoann</creatorcontrib><creatorcontrib>Motte, Laurence</creatorcontrib><title>Easily Controlled Grafting of Oligonucleotides on gamma Fe sub(2)O sub(3) Nanoparticles: Physicochemical Characterization of DNA Organization and Biological Activity Studies</title><title>The journal of physical chemistry. B</title><description>We report a one-step process to functionalize superparamagnetic iron oxide nanoparticle (SPIO-NP) surfaces with a controlled number of oligonucleotides. For this study, we use a specific oligonucleotide targeting the signal transducer and activator of transcription 3 (STAT3), a key regulator of cell survival and proliferation. This oligonucleotide is self-complementary and can adopt a hairpin structure. It is labeled with the fluorescein amidite group at the 3'-end. The polyanionic DNA is electrostatically attracted onto the positively charged surface of the bare SPIO-NPs. During synthesis, the molar ratio between the oligonucleotides and nanoparticles was varied from 17.5 to 175. For particles with a mean diameter of 10 nm, a nanoparticle surface saturation is observed corresponding to 70 DNA strands per particle. The increase of DNA density per nanoparticle is correlated to a transition from the hairpin structure adsorbed horizontally on the nanoparticle surface to a vertically ordered surface packing assembly. An in vitro study on human colon carcinoma cell line SW480 shows that the kinetics of internalization and biological activity of the NPs seem to be dependent on the oligonucleotide density. Cell death and the kinetics of internalization are favored by a low density of oligonucleotides.</description><subject>Biological</subject><subject>Colon</subject><subject>Density</subject><subject>Deoxyribonucleic acid</subject><subject>Nanoparticles</subject><subject>Oligonucleotides</subject><subject>Surface chemistry</subject><subject>Transducers</subject><issn>1520-6106</issn><issn>1520-5207</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNqFjLFOwzAYhCMEEqUw8AYe2yFgx3HisJXSFqSqQaJ79cexU1dOHGIHqbwT70hUYGY43el09wXBLcF3BEfk_tDGBPMsg7NgRFiEw0Hp-W9OCE4ugyvnDhhHLOLJKPhagNPmiOa28Z01RpZo1YHyuqmQVSg3urJNL4y0XpfSIdugCuoa0FIi1xeTaJqfnE7RBhrbQuf1sHYP6HV_dFpYsZe1FmDQfA8dCC87_QleD5wB_7SZobyroPnroCnRo7bGVqfPTHj9of0Rvfm-1NJdBxcKjJM3vz4OtsvFdv4crvPVy3y2DtskoaEsEywET5QgKRVFJghwUjAiKSWc05iWGcFECEVxTBkupMpUmgIWBbBYYULHweQH23b2vZfO72rthDQGGml7tyMpoyzKeEL_nzIac0IZTek3ToCBGw</recordid><startdate>20140203</startdate><enddate>20140203</enddate><creator>Geinguenaud, Frederic</creator><creator>Souissi, Ines</creator><creator>Fagard, Remi</creator><creator>Lalatonne, Yoann</creator><creator>Motte, Laurence</creator><scope>7TM</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20140203</creationdate><title>Easily Controlled Grafting of Oligonucleotides on gamma Fe sub(2)O sub(3) Nanoparticles: Physicochemical Characterization of DNA Organization and Biological Activity Studies</title><author>Geinguenaud, Frederic ; Souissi, Ines ; Fagard, Remi ; Lalatonne, Yoann ; Motte, Laurence</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p663-ed60cc86fc173cb9c1a81b51e33188343d9101ccf304350bef9f77a0cba54f013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Biological</topic><topic>Colon</topic><topic>Density</topic><topic>Deoxyribonucleic acid</topic><topic>Nanoparticles</topic><topic>Oligonucleotides</topic><topic>Surface chemistry</topic><topic>Transducers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Geinguenaud, Frederic</creatorcontrib><creatorcontrib>Souissi, Ines</creatorcontrib><creatorcontrib>Fagard, Remi</creatorcontrib><creatorcontrib>Lalatonne, Yoann</creatorcontrib><creatorcontrib>Motte, Laurence</creatorcontrib><collection>Nucleic Acids Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>The journal of physical chemistry. B</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Geinguenaud, Frederic</au><au>Souissi, Ines</au><au>Fagard, Remi</au><au>Lalatonne, Yoann</au><au>Motte, Laurence</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Easily Controlled Grafting of Oligonucleotides on gamma Fe sub(2)O sub(3) Nanoparticles: Physicochemical Characterization of DNA Organization and Biological Activity Studies</atitle><jtitle>The journal of physical chemistry. B</jtitle><date>2014-02-03</date><risdate>2014</risdate><volume>118</volume><issue>6</issue><spage>1535</spage><epage>1544-1535-1544</epage><pages>1535-1544-1535-1544</pages><issn>1520-6106</issn><eissn>1520-5207</eissn><abstract>We report a one-step process to functionalize superparamagnetic iron oxide nanoparticle (SPIO-NP) surfaces with a controlled number of oligonucleotides. For this study, we use a specific oligonucleotide targeting the signal transducer and activator of transcription 3 (STAT3), a key regulator of cell survival and proliferation. This oligonucleotide is self-complementary and can adopt a hairpin structure. It is labeled with the fluorescein amidite group at the 3'-end. The polyanionic DNA is electrostatically attracted onto the positively charged surface of the bare SPIO-NPs. During synthesis, the molar ratio between the oligonucleotides and nanoparticles was varied from 17.5 to 175. For particles with a mean diameter of 10 nm, a nanoparticle surface saturation is observed corresponding to 70 DNA strands per particle. The increase of DNA density per nanoparticle is correlated to a transition from the hairpin structure adsorbed horizontally on the nanoparticle surface to a vertically ordered surface packing assembly. An in vitro study on human colon carcinoma cell line SW480 shows that the kinetics of internalization and biological activity of the NPs seem to be dependent on the oligonucleotide density. Cell death and the kinetics of internalization are favored by a low density of oligonucleotides.</abstract><doi>10.1021/jp410899a</doi></addata></record>
fulltext fulltext
identifier ISSN: 1520-6106
ispartof The journal of physical chemistry. B, 2014-02, Vol.118 (6), p.1535-1544-1535-1544
issn 1520-6106
1520-5207
language eng
recordid cdi_proquest_miscellaneous_1753529863
source American Chemical Society Journals
subjects Biological
Colon
Density
Deoxyribonucleic acid
Nanoparticles
Oligonucleotides
Surface chemistry
Transducers
title Easily Controlled Grafting of Oligonucleotides on gamma Fe sub(2)O sub(3) Nanoparticles: Physicochemical Characterization of DNA Organization and Biological Activity Studies
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T17%3A20%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Easily%20Controlled%20Grafting%20of%20Oligonucleotides%20on%20gamma%20Fe%20sub(2)O%20sub(3)%20Nanoparticles:%20Physicochemical%20Characterization%20of%20DNA%20Organization%20and%20Biological%20Activity%20Studies&rft.jtitle=The%20journal%20of%20physical%20chemistry.%20B&rft.au=Geinguenaud,%20Frederic&rft.date=2014-02-03&rft.volume=118&rft.issue=6&rft.spage=1535&rft.epage=1544-1535-1544&rft.pages=1535-1544-1535-1544&rft.issn=1520-6106&rft.eissn=1520-5207&rft_id=info:doi/10.1021/jp410899a&rft_dat=%3Cproquest%3E1753529863%3C/proquest%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1534813537&rft_id=info:pmid/&rfr_iscdi=true