DNA Interaction with Diaminobenzidine Studied with Optical Tweezers and Dynamic Light Scattering
We have studied the interaction of the DNA molecule with the ligand 3,3′-diaminobenzidine (DAB) by performing single molecule stretching experiments with optical tweezers and dynamic light scattering (DLS) on the DNA–DAB complexes. In the stretching experiments, the persistence and contour lengths o...
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| Veröffentlicht in: | The journal of physical chemistry. B 2013-11, Vol.117 (46), p.14345-14350 |
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| container_title | The journal of physical chemistry. B |
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| creator | Reis, L. A Ramos, E. B Rocha, M. S |
| description | We have studied the interaction of the DNA molecule with the ligand 3,3′-diaminobenzidine (DAB) by performing single molecule stretching experiments with optical tweezers and dynamic light scattering (DLS) on the DNA–DAB complexes. In the stretching experiments, the persistence and contour lengths of the complexes were measured as a function of DAB concentration, allowing one to infer the main binding mechanism and also to determine the physicochemical parameters of the interaction. In the DLS experiments, the effective size of the complexes, measured as the hydrodynamic radius, was monitored as a function of DAB concentration. We found a qualitative agreement between the results obtained from the two techniques by comparing the behaviors of the hydrodynamics radius and the radius of gyration, since this last one can be expressed as a function of the persistence and contour lengths. |
| doi_str_mv | 10.1021/jp409544e |
| format | Article |
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A</creatorcontrib><creatorcontrib>Ramos, E. B</creatorcontrib><creatorcontrib>Rocha, M. S</creatorcontrib><title>DNA Interaction with Diaminobenzidine Studied with Optical Tweezers and Dynamic Light Scattering</title><title>The journal of physical chemistry. B</title><addtitle>J. Phys. Chem. B</addtitle><description>We have studied the interaction of the DNA molecule with the ligand 3,3′-diaminobenzidine (DAB) by performing single molecule stretching experiments with optical tweezers and dynamic light scattering (DLS) on the DNA–DAB complexes. In the stretching experiments, the persistence and contour lengths of the complexes were measured as a function of DAB concentration, allowing one to infer the main binding mechanism and also to determine the physicochemical parameters of the interaction. In the DLS experiments, the effective size of the complexes, measured as the hydrodynamic radius, was monitored as a function of DAB concentration. We found a qualitative agreement between the results obtained from the two techniques by comparing the behaviors of the hydrodynamics radius and the radius of gyration, since this last one can be expressed as a function of the persistence and contour lengths.</description><subject>Benzidines - chemistry</subject><subject>Biological and medical sciences</subject><subject>Deoxyribonucleic acid</subject><subject>DNA - chemistry</subject><subject>DNA - metabolism</subject><subject>Dynamic tests</subject><subject>Dynamics</subject><subject>Fluid dynamics</subject><subject>Fluid flow</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrodynamics</subject><subject>Intermolecular phenomena</subject><subject>Ligands</subject><subject>Light</subject><subject>Molecular biophysics</subject><subject>Optical Tweezers</subject><subject>Scattering, Radiation</subject><subject>Shape</subject><subject>Stretching</subject><issn>1520-6106</issn><issn>1520-5207</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU9PGzEQxa2qqFDooV-g8gWpPQRsr_9sjoi0BSmCA3DeztpjcJR4g-0Vgk9fVwlwQaIHayzNb95o3iPkK2dHnAl-vFhLNlVS4geyx5Vgk_rMx-1fc6Z3yeecF4wJJVr9iewKybVsmNgjf2YXJ_Q8FkxgSxgifQjljs4CrEIceoxPwYWI9KqMLqDbdC_XJVhY0usHxCdMmUJ0dPYY64yl83B7V-iVhVI1Q7w9IDselhm_bOs-ufn18_r0bDK__H1-ejKfgJS6TEQvW65QO14v0br1GqzzvBGyF8AUM41vUBqPrWyN170DUOh7zXqH3AE2--T7RnedhvsRc-lWIVtcLiHiMOaOG9UooVvD3kdVI1shjPgPVE6nnBvGTUV_bFCbhpwT-m6dwgrSY8dZ9y-m7iWmyn7byo79Ct0L-ZxLBQ63AORqtU8QbcivXFsNacz0lQObu8Uwplg9fmPhX1TFpXk</recordid><startdate>20131121</startdate><enddate>20131121</enddate><creator>Reis, L. 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S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a446t-2b4815e6d1095668f6acdf1324b2a05073f3e47fe8487f6bdaa5efb60bde1dae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Benzidines - chemistry</topic><topic>Biological and medical sciences</topic><topic>Deoxyribonucleic acid</topic><topic>DNA - chemistry</topic><topic>DNA - metabolism</topic><topic>Dynamic tests</topic><topic>Dynamics</topic><topic>Fluid dynamics</topic><topic>Fluid flow</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrodynamics</topic><topic>Intermolecular phenomena</topic><topic>Ligands</topic><topic>Light</topic><topic>Molecular biophysics</topic><topic>Optical Tweezers</topic><topic>Scattering, Radiation</topic><topic>Shape</topic><topic>Stretching</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Reis, L. 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B</au><au>Rocha, M. S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA Interaction with Diaminobenzidine Studied with Optical Tweezers and Dynamic Light Scattering</atitle><jtitle>The journal of physical chemistry. B</jtitle><addtitle>J. Phys. Chem. B</addtitle><date>2013-11-21</date><risdate>2013</risdate><volume>117</volume><issue>46</issue><spage>14345</spage><epage>14350</epage><pages>14345-14350</pages><issn>1520-6106</issn><eissn>1520-5207</eissn><abstract>We have studied the interaction of the DNA molecule with the ligand 3,3′-diaminobenzidine (DAB) by performing single molecule stretching experiments with optical tweezers and dynamic light scattering (DLS) on the DNA–DAB complexes. In the stretching experiments, the persistence and contour lengths of the complexes were measured as a function of DAB concentration, allowing one to infer the main binding mechanism and also to determine the physicochemical parameters of the interaction. In the DLS experiments, the effective size of the complexes, measured as the hydrodynamic radius, was monitored as a function of DAB concentration. We found a qualitative agreement between the results obtained from the two techniques by comparing the behaviors of the hydrodynamics radius and the radius of gyration, since this last one can be expressed as a function of the persistence and contour lengths.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>24164302</pmid><doi>10.1021/jp409544e</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Benzidines - chemistry Biological and medical sciences Deoxyribonucleic acid DNA - chemistry DNA - metabolism Dynamic tests Dynamics Fluid dynamics Fluid flow Fundamental and applied biological sciences. Psychology Hydrodynamics Intermolecular phenomena Ligands Light Molecular biophysics Optical Tweezers Scattering, Radiation Shape Stretching |
| title | DNA Interaction with Diaminobenzidine Studied with Optical Tweezers and Dynamic Light Scattering |
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