pH-Sensitive polymer assisted self-aggregation of bis(pyrene) in living cells in situ with turn-on fluorescence
Supramolecular self-assemblies with various nanostructures in organic and aqueous solutions have been prepared with desired functions. However, in situ construction of self-assembled superstructures in physiological conditions to achieve expected biological functions remains a challenge. Here, we re...
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| Veröffentlicht in: | Nanotechnology 2015-09, Vol.26 (35), p.355703-11 |
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| container_title | Nanotechnology |
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| creator | Duan, Zhongyu Gao, Yu-Juan Qiao, Zeng-Ying Qiao, Shenglin Wang, Yongmei Hou, Chunyuan Wang, Lei Wang, Hao |
| description | Supramolecular self-assemblies with various nanostructures in organic and aqueous solutions have been prepared with desired functions. However, in situ construction of self-assembled superstructures in physiological conditions to achieve expected biological functions remains a challenge. Here, we report a supramolecular system to realize the in situ formation of nanoaggregates in living cells. The bis(pyrene) monomers were dispersed inside of hydrophobic domains of pH-sensitive polymeric micelles and delivered to the lysosomes of cells. In the acidic lysosomes, the bis(pyrene) monomers were released and self-aggregated with turn-on fluorescence. We envision this strategy for in situ construction of supramolecular nanostructures in living cells will pave the way for molecular diagnostics in the future. |
| doi_str_mv | 10.1088/0957-4484/26/35/355703 |
| format | Article |
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However, in situ construction of self-assembled superstructures in physiological conditions to achieve expected biological functions remains a challenge. Here, we report a supramolecular system to realize the in situ formation of nanoaggregates in living cells. The bis(pyrene) monomers were dispersed inside of hydrophobic domains of pH-sensitive polymeric micelles and delivered to the lysosomes of cells. In the acidic lysosomes, the bis(pyrene) monomers were released and self-aggregated with turn-on fluorescence. We envision this strategy for in situ construction of supramolecular nanostructures in living cells will pave the way for molecular diagnostics in the future.</description><identifier>ISSN: 0957-4484</identifier><identifier>EISSN: 1361-6528</identifier><identifier>DOI: 10.1088/0957-4484/26/35/355703</identifier><identifier>PMID: 26245834</identifier><identifier>CODEN: NNOTER</identifier><language>eng</language><publisher>England: IOP Publishing</publisher><subject>bis(pyrene) ; Construction ; Cytological Techniques - methods ; Fluorescence ; Fluorescent Dyes - chemistry ; Fluorescent Dyes - metabolism ; HeLa Cells ; Humans ; Hydrogen-Ion Concentration ; living cells ; Lysosomes ; Monomers ; Nanostructure ; Nanostructures - chemistry ; Polymers - chemistry ; Polymers - metabolism ; Pyrenes - chemistry ; Pyrenes - metabolism ; Self assembly ; Strategy ; Superstructures</subject><ispartof>Nanotechnology, 2015-09, Vol.26 (35), p.355703-11</ispartof><rights>2015 IOP Publishing Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-a66b561271a20bb562d78f1c4351bddab6e2fee6cfae26e42c3d924d9bc257e03</citedby><cites>FETCH-LOGICAL-c421t-a66b561271a20bb562d78f1c4351bddab6e2fee6cfae26e42c3d924d9bc257e03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://iopscience.iop.org/article/10.1088/0957-4484/26/35/355703/pdf$$EPDF$$P50$$Giop$$H</linktopdf><link.rule.ids>314,780,784,27924,27925,53846,53893</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26245834$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Duan, Zhongyu</creatorcontrib><creatorcontrib>Gao, Yu-Juan</creatorcontrib><creatorcontrib>Qiao, Zeng-Ying</creatorcontrib><creatorcontrib>Qiao, Shenglin</creatorcontrib><creatorcontrib>Wang, Yongmei</creatorcontrib><creatorcontrib>Hou, Chunyuan</creatorcontrib><creatorcontrib>Wang, Lei</creatorcontrib><creatorcontrib>Wang, Hao</creatorcontrib><title>pH-Sensitive polymer assisted self-aggregation of bis(pyrene) in living cells in situ with turn-on fluorescence</title><title>Nanotechnology</title><addtitle>NANO</addtitle><addtitle>Nanotechnology</addtitle><description>Supramolecular self-assemblies with various nanostructures in organic and aqueous solutions have been prepared with desired functions. However, in situ construction of self-assembled superstructures in physiological conditions to achieve expected biological functions remains a challenge. Here, we report a supramolecular system to realize the in situ formation of nanoaggregates in living cells. The bis(pyrene) monomers were dispersed inside of hydrophobic domains of pH-sensitive polymeric micelles and delivered to the lysosomes of cells. In the acidic lysosomes, the bis(pyrene) monomers were released and self-aggregated with turn-on fluorescence. We envision this strategy for in situ construction of supramolecular nanostructures in living cells will pave the way for molecular diagnostics in the future.</description><subject>bis(pyrene)</subject><subject>Construction</subject><subject>Cytological Techniques - methods</subject><subject>Fluorescence</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Fluorescent Dyes - metabolism</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>living cells</subject><subject>Lysosomes</subject><subject>Monomers</subject><subject>Nanostructure</subject><subject>Nanostructures - chemistry</subject><subject>Polymers - chemistry</subject><subject>Polymers - metabolism</subject><subject>Pyrenes - chemistry</subject><subject>Pyrenes - metabolism</subject><subject>Self assembly</subject><subject>Strategy</subject><subject>Superstructures</subject><issn>0957-4484</issn><issn>1361-6528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkF1rFDEUhoModl39CyWX7cW4ycnHzF5KsVYoeKFeh0zmZE2ZTcZkprL_3gxbC4IgBJLA8745eQi55Ow9Z123Y3vVNlJ2cgd6J1RdqmXiBdlwoXmjFXQvyeYZuiBvSnlgjPMO-GtyARqk6oTckDTdNV8xljCHR6RTGk9HzNSWEsqMAy04-sYeDhkPdg4p0uRpH8rVdMoY8ZqGSMfwGOKBOhzHst5r1UJ_hfkHnZccm5rx45IyFofR4Vvyytux4LunfUu-3378dnPX3H_59Pnmw33jJPC5sVr3SnNouQXW1yMMbee5k0LxfhhsrxE8onbeImiU4MSwBznseweqRSa25OrcO-X0c8Eym2Mo64w2YlqK4a0SCrSqFrZEn1GXUykZvZlyONp8MpyZVbZZPZrVowFthDJn2TV4-fTG0h9xeI79sVuB6zMQ0mQeUtVRv2yijemvIjMNvrLwD_Y_E_wGCcqZBA</recordid><startdate>20150904</startdate><enddate>20150904</enddate><creator>Duan, Zhongyu</creator><creator>Gao, Yu-Juan</creator><creator>Qiao, Zeng-Ying</creator><creator>Qiao, Shenglin</creator><creator>Wang, Yongmei</creator><creator>Hou, Chunyuan</creator><creator>Wang, Lei</creator><creator>Wang, Hao</creator><general>IOP Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20150904</creationdate><title>pH-Sensitive polymer assisted self-aggregation of bis(pyrene) in living cells in situ with turn-on fluorescence</title><author>Duan, Zhongyu ; Gao, Yu-Juan ; Qiao, Zeng-Ying ; Qiao, Shenglin ; Wang, Yongmei ; Hou, Chunyuan ; Wang, Lei ; Wang, Hao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c421t-a66b561271a20bb562d78f1c4351bddab6e2fee6cfae26e42c3d924d9bc257e03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>bis(pyrene)</topic><topic>Construction</topic><topic>Cytological Techniques - methods</topic><topic>Fluorescence</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Fluorescent Dyes - metabolism</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>living cells</topic><topic>Lysosomes</topic><topic>Monomers</topic><topic>Nanostructure</topic><topic>Nanostructures - chemistry</topic><topic>Polymers - chemistry</topic><topic>Polymers - metabolism</topic><topic>Pyrenes - chemistry</topic><topic>Pyrenes - metabolism</topic><topic>Self assembly</topic><topic>Strategy</topic><topic>Superstructures</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Duan, Zhongyu</creatorcontrib><creatorcontrib>Gao, Yu-Juan</creatorcontrib><creatorcontrib>Qiao, Zeng-Ying</creatorcontrib><creatorcontrib>Qiao, Shenglin</creatorcontrib><creatorcontrib>Wang, Yongmei</creatorcontrib><creatorcontrib>Hou, Chunyuan</creatorcontrib><creatorcontrib>Wang, Lei</creatorcontrib><creatorcontrib>Wang, Hao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Nanotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Duan, Zhongyu</au><au>Gao, Yu-Juan</au><au>Qiao, Zeng-Ying</au><au>Qiao, Shenglin</au><au>Wang, Yongmei</au><au>Hou, Chunyuan</au><au>Wang, Lei</au><au>Wang, Hao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>pH-Sensitive polymer assisted self-aggregation of bis(pyrene) in living cells in situ with turn-on fluorescence</atitle><jtitle>Nanotechnology</jtitle><stitle>NANO</stitle><addtitle>Nanotechnology</addtitle><date>2015-09-04</date><risdate>2015</risdate><volume>26</volume><issue>35</issue><spage>355703</spage><epage>11</epage><pages>355703-11</pages><issn>0957-4484</issn><eissn>1361-6528</eissn><coden>NNOTER</coden><abstract>Supramolecular self-assemblies with various nanostructures in organic and aqueous solutions have been prepared with desired functions. However, in situ construction of self-assembled superstructures in physiological conditions to achieve expected biological functions remains a challenge. Here, we report a supramolecular system to realize the in situ formation of nanoaggregates in living cells. The bis(pyrene) monomers were dispersed inside of hydrophobic domains of pH-sensitive polymeric micelles and delivered to the lysosomes of cells. In the acidic lysosomes, the bis(pyrene) monomers were released and self-aggregated with turn-on fluorescence. We envision this strategy for in situ construction of supramolecular nanostructures in living cells will pave the way for molecular diagnostics in the future.</abstract><cop>England</cop><pub>IOP Publishing</pub><pmid>26245834</pmid><doi>10.1088/0957-4484/26/35/355703</doi><tpages>11</tpages></addata></record> |
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| subjects | bis(pyrene) Construction Cytological Techniques - methods Fluorescence Fluorescent Dyes - chemistry Fluorescent Dyes - metabolism HeLa Cells Humans Hydrogen-Ion Concentration living cells Lysosomes Monomers Nanostructure Nanostructures - chemistry Polymers - chemistry Polymers - metabolism Pyrenes - chemistry Pyrenes - metabolism Self assembly Strategy Superstructures |
| title | pH-Sensitive polymer assisted self-aggregation of bis(pyrene) in living cells in situ with turn-on fluorescence |
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