β-Carotene and protein oxidation: effects of ascorbic acid and α-tocopherol
The effect of β-carotene on protein oxidation was examined under different oxygen (O 2) tensions and with other antioxidants: α-tocopherol, ascorbic acid, and mixtures of antioxidants. Human serum albumin (HSA) was incubated with 2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH) to induce protei...
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| Veröffentlicht in: | Toxicology (Amsterdam) 2000-04, Vol.146 (1), p.37-47 |
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| creator | Zhang, Peng Omaye, Stanley T. |
| description | The effect of β-carotene on protein oxidation was examined under different oxygen (O
2) tensions and with other antioxidants: α-tocopherol, ascorbic acid, and mixtures of antioxidants. Human serum albumin (HSA) was incubated with 2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH) to induce protein oxidation (carbonyl formation), under 15, 150, and 760 torr of O
2 tension. Antioxidant activity was related to O
2 tension, antioxidant concentrations and interaction between mixtures of antioxidants: (1) Under 15 torr of O
2, incubating HSA with AAPH, 1.6 μM β-carotene, 80 μM α-tocopherol, 160 μM ascorbic acid, and mixtures (0.1 μM β-carotene, 5.0 μM α-tocopherol and 10 μM ascorbic acid) resulted in 24, 29, 39, and 44% reduction of carbonyl formation, respectively. (2) Under 150 torr of O
2 tension, the antioxidant effect of β-carotene was decreased by 4% but increasing O
2 tension did not diminish the antioxidant effects of α-tocopherol, ascorbic acid, or antioxidant mixtures. (3). Under 760 torr of O
2 tension, adding 1.6 μM β-carotene resulted in 26% more carbonyl formation. (4) Under 760 torr of O
2 tension, the antioxidant effect of ascorbic acid was decreased 32% compared to what was observed at 150 torr of O
2 tension. Changes in O
2 tension had no effect on the antioxidant effect of α-tocopherol. The mixture of antioxidants inhibited carbonyl formation by 37% and was 7% less effective than that of 15 and 150 torr of O
2 tension. High concentration of β-carotene produces more protein oxidation in the presence of high O
2 tension by a prooxidant mechanism. Mixtures of β-carotene, α-tocopherol, and ascorbic acid provided better protective effects on protein oxidation than any single compound. |
| doi_str_mv | 10.1016/S0300-483X(00)00160-8 |
| format | Article |
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2) tensions and with other antioxidants: α-tocopherol, ascorbic acid, and mixtures of antioxidants. Human serum albumin (HSA) was incubated with 2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH) to induce protein oxidation (carbonyl formation), under 15, 150, and 760 torr of O
2 tension. Antioxidant activity was related to O
2 tension, antioxidant concentrations and interaction between mixtures of antioxidants: (1) Under 15 torr of O
2, incubating HSA with AAPH, 1.6 μM β-carotene, 80 μM α-tocopherol, 160 μM ascorbic acid, and mixtures (0.1 μM β-carotene, 5.0 μM α-tocopherol and 10 μM ascorbic acid) resulted in 24, 29, 39, and 44% reduction of carbonyl formation, respectively. (2) Under 150 torr of O
2 tension, the antioxidant effect of β-carotene was decreased by 4% but increasing O
2 tension did not diminish the antioxidant effects of α-tocopherol, ascorbic acid, or antioxidant mixtures. (3). Under 760 torr of O
2 tension, adding 1.6 μM β-carotene resulted in 26% more carbonyl formation. (4) Under 760 torr of O
2 tension, the antioxidant effect of ascorbic acid was decreased 32% compared to what was observed at 150 torr of O
2 tension. Changes in O
2 tension had no effect on the antioxidant effect of α-tocopherol. The mixture of antioxidants inhibited carbonyl formation by 37% and was 7% less effective than that of 15 and 150 torr of O
2 tension. High concentration of β-carotene produces more protein oxidation in the presence of high O
2 tension by a prooxidant mechanism. Mixtures of β-carotene, α-tocopherol, and ascorbic acid provided better protective effects on protein oxidation than any single compound.</description><identifier>ISSN: 0300-483X</identifier><identifier>EISSN: 1879-3185</identifier><identifier>DOI: 10.1016/S0300-483X(00)00160-8</identifier><identifier>PMID: 10773361</identifier><identifier>CODEN: TXICDD</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>a-Tocopherol ; Amidines - chemistry ; Antioxidant ; Antioxidants - chemistry ; ascorbic acid ; Ascorbic acid (vitamin C) ; Ascorbic Acid - chemistry ; b-Carotene ; beta Carotene - chemistry ; Biological and medical sciences ; Enzyme-Linked Immunosorbent Assay ; General and cellular metabolism. Vitamins ; Humans ; Medical sciences ; Oxidants - chemistry ; oxidation ; Oxidation-Reduction ; Oxygen - chemistry ; Pharmacology. Drug treatments ; Phenylhydrazines - chemistry ; Prooxidant ; Protein oxidation ; proteins ; Serum Albumin - chemistry ; Vitamin E - chemistry ; α-Tocopherol (vitamin E) ; β-Carotene</subject><ispartof>Toxicology (Amsterdam), 2000-04, Vol.146 (1), p.37-47</ispartof><rights>2000 Elsevier Science Ireland Ltd</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-79a44312e2f1221513150767251177d1ffefaa7385c135d7c883170157824d803</citedby><cites>FETCH-LOGICAL-c421t-79a44312e2f1221513150767251177d1ffefaa7385c135d7c883170157824d803</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0300-483X(00)00160-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1395926$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10773361$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Peng</creatorcontrib><creatorcontrib>Omaye, Stanley T.</creatorcontrib><title>β-Carotene and protein oxidation: effects of ascorbic acid and α-tocopherol</title><title>Toxicology (Amsterdam)</title><addtitle>Toxicology</addtitle><description>The effect of β-carotene on protein oxidation was examined under different oxygen (O
2) tensions and with other antioxidants: α-tocopherol, ascorbic acid, and mixtures of antioxidants. Human serum albumin (HSA) was incubated with 2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH) to induce protein oxidation (carbonyl formation), under 15, 150, and 760 torr of O
2 tension. Antioxidant activity was related to O
2 tension, antioxidant concentrations and interaction between mixtures of antioxidants: (1) Under 15 torr of O
2, incubating HSA with AAPH, 1.6 μM β-carotene, 80 μM α-tocopherol, 160 μM ascorbic acid, and mixtures (0.1 μM β-carotene, 5.0 μM α-tocopherol and 10 μM ascorbic acid) resulted in 24, 29, 39, and 44% reduction of carbonyl formation, respectively. (2) Under 150 torr of O
2 tension, the antioxidant effect of β-carotene was decreased by 4% but increasing O
2 tension did not diminish the antioxidant effects of α-tocopherol, ascorbic acid, or antioxidant mixtures. (3). Under 760 torr of O
2 tension, adding 1.6 μM β-carotene resulted in 26% more carbonyl formation. (4) Under 760 torr of O
2 tension, the antioxidant effect of ascorbic acid was decreased 32% compared to what was observed at 150 torr of O
2 tension. Changes in O
2 tension had no effect on the antioxidant effect of α-tocopherol. The mixture of antioxidants inhibited carbonyl formation by 37% and was 7% less effective than that of 15 and 150 torr of O
2 tension. High concentration of β-carotene produces more protein oxidation in the presence of high O
2 tension by a prooxidant mechanism. Mixtures of β-carotene, α-tocopherol, and ascorbic acid provided better protective effects on protein oxidation than any single compound.</description><subject>a-Tocopherol</subject><subject>Amidines - chemistry</subject><subject>Antioxidant</subject><subject>Antioxidants - chemistry</subject><subject>ascorbic acid</subject><subject>Ascorbic acid (vitamin C)</subject><subject>Ascorbic Acid - chemistry</subject><subject>b-Carotene</subject><subject>beta Carotene - chemistry</subject><subject>Biological and medical sciences</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>General and cellular metabolism. Vitamins</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Oxidants - chemistry</subject><subject>oxidation</subject><subject>Oxidation-Reduction</subject><subject>Oxygen - chemistry</subject><subject>Pharmacology. Drug treatments</subject><subject>Phenylhydrazines - chemistry</subject><subject>Prooxidant</subject><subject>Protein oxidation</subject><subject>proteins</subject><subject>Serum Albumin - chemistry</subject><subject>Vitamin E - chemistry</subject><subject>α-Tocopherol (vitamin E)</subject><subject>β-Carotene</subject><issn>0300-483X</issn><issn>1879-3185</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM9O3DAQhy1UBFvKI1DlUFXlEDpjx7HDBVWrUpCoemgrcbOM_wijbLy1sxU8VnkQnqnezar0xmlGo29mfvoIOUI4QcD243dgAHUj2fUHgGMoI6jlDpmhFF3NUPJXZPYP2Sevc74DAMqado_sIwjBWIsz8vXpsZ7rFEc3uEoPtlqu-zBU8T5YPYY4nFbOe2fGXEVf6Wxiugmm0ibYDf_0px6jictbl2L_hux63Wd3uK0H5Of55x_zi_rq25fL-aer2jQUx1p0umkYUkc9UoocGXIQraAcUQiL5Z_XWjDJDTJuhZGSoQDkQtLGSmAH5P10t6T9tXJ5VIuQjet7Pbi4ygoFZ7wYKSCfQJNizsl5tUxhodODQlBrj2rjUa0lqVI3HpUse2-3D1Y3C2f_25rEFeDdFihKdO-THkzIzxzreEfbgp1NmCs2fgeXVDbBDcbZkIpTZWN4IclfhO6OHw</recordid><startdate>20000420</startdate><enddate>20000420</enddate><creator>Zhang, Peng</creator><creator>Omaye, Stanley T.</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20000420</creationdate><title>β-Carotene and protein oxidation: effects of ascorbic acid and α-tocopherol</title><author>Zhang, Peng ; Omaye, Stanley T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c421t-79a44312e2f1221513150767251177d1ffefaa7385c135d7c883170157824d803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>a-Tocopherol</topic><topic>Amidines - chemistry</topic><topic>Antioxidant</topic><topic>Antioxidants - chemistry</topic><topic>ascorbic acid</topic><topic>Ascorbic acid (vitamin C)</topic><topic>Ascorbic Acid - chemistry</topic><topic>b-Carotene</topic><topic>beta Carotene - chemistry</topic><topic>Biological and medical sciences</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>General and cellular metabolism. Vitamins</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Oxidants - chemistry</topic><topic>oxidation</topic><topic>Oxidation-Reduction</topic><topic>Oxygen - chemistry</topic><topic>Pharmacology. Drug treatments</topic><topic>Phenylhydrazines - chemistry</topic><topic>Prooxidant</topic><topic>Protein oxidation</topic><topic>proteins</topic><topic>Serum Albumin - chemistry</topic><topic>Vitamin E - chemistry</topic><topic>α-Tocopherol (vitamin E)</topic><topic>β-Carotene</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Peng</creatorcontrib><creatorcontrib>Omaye, Stanley T.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Toxicology (Amsterdam)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Peng</au><au>Omaye, Stanley T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>β-Carotene and protein oxidation: effects of ascorbic acid and α-tocopherol</atitle><jtitle>Toxicology (Amsterdam)</jtitle><addtitle>Toxicology</addtitle><date>2000-04-20</date><risdate>2000</risdate><volume>146</volume><issue>1</issue><spage>37</spage><epage>47</epage><pages>37-47</pages><issn>0300-483X</issn><eissn>1879-3185</eissn><coden>TXICDD</coden><abstract>The effect of β-carotene on protein oxidation was examined under different oxygen (O
2) tensions and with other antioxidants: α-tocopherol, ascorbic acid, and mixtures of antioxidants. Human serum albumin (HSA) was incubated with 2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH) to induce protein oxidation (carbonyl formation), under 15, 150, and 760 torr of O
2 tension. Antioxidant activity was related to O
2 tension, antioxidant concentrations and interaction between mixtures of antioxidants: (1) Under 15 torr of O
2, incubating HSA with AAPH, 1.6 μM β-carotene, 80 μM α-tocopherol, 160 μM ascorbic acid, and mixtures (0.1 μM β-carotene, 5.0 μM α-tocopherol and 10 μM ascorbic acid) resulted in 24, 29, 39, and 44% reduction of carbonyl formation, respectively. (2) Under 150 torr of O
2 tension, the antioxidant effect of β-carotene was decreased by 4% but increasing O
2 tension did not diminish the antioxidant effects of α-tocopherol, ascorbic acid, or antioxidant mixtures. (3). Under 760 torr of O
2 tension, adding 1.6 μM β-carotene resulted in 26% more carbonyl formation. (4) Under 760 torr of O
2 tension, the antioxidant effect of ascorbic acid was decreased 32% compared to what was observed at 150 torr of O
2 tension. Changes in O
2 tension had no effect on the antioxidant effect of α-tocopherol. The mixture of antioxidants inhibited carbonyl formation by 37% and was 7% less effective than that of 15 and 150 torr of O
2 tension. High concentration of β-carotene produces more protein oxidation in the presence of high O
2 tension by a prooxidant mechanism. Mixtures of β-carotene, α-tocopherol, and ascorbic acid provided better protective effects on protein oxidation than any single compound.</abstract><cop>Shannon</cop><cop>Amsterdam</cop><pub>Elsevier Ireland Ltd</pub><pmid>10773361</pmid><doi>10.1016/S0300-483X(00)00160-8</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0300-483X |
| ispartof | Toxicology (Amsterdam), 2000-04, Vol.146 (1), p.37-47 |
| issn | 0300-483X 1879-3185 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | a-Tocopherol Amidines - chemistry Antioxidant Antioxidants - chemistry ascorbic acid Ascorbic acid (vitamin C) Ascorbic Acid - chemistry b-Carotene beta Carotene - chemistry Biological and medical sciences Enzyme-Linked Immunosorbent Assay General and cellular metabolism. Vitamins Humans Medical sciences Oxidants - chemistry oxidation Oxidation-Reduction Oxygen - chemistry Pharmacology. Drug treatments Phenylhydrazines - chemistry Prooxidant Protein oxidation proteins Serum Albumin - chemistry Vitamin E - chemistry α-Tocopherol (vitamin E) β-Carotene |
| title | β-Carotene and protein oxidation: effects of ascorbic acid and α-tocopherol |
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