DNA Interaction with Hoechst 33258: Stretching Experiments Decouple the Different Binding Modes

By performing single molecule stretching experiments with optical tweezers, we have studied the DNA interaction with the ligand Hoechst 33258. The mechanical properties of the complexes formed as a function of ligand concentration were directly determined from these measurements by fitting the force...

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Veröffentlicht in:	The journal of physical chemistry. B 2013-06, Vol.117 (24), p.7292-7296
Hauptverfasser:	Silva, E. F, Ramos, E. B, Rocha, M. S
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriophage lambda - chemistry Binding Binding Sites Biological and medical sciences Bisbenzimidazole - chemistry Deoxyribonucleic acid DNA, Viral - chemistry Fundamental and applied biological sciences. Psychology Interactions. Associations Intermolecular phenomena Isotherms Ligands Mathematical models Molecular biophysics Quenching (cooling) Shape Stretching
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