Rapid tea catechins and caffeine determination by HPLC using microwave-assisted extraction and silica monolithic column
•Development of a rapid HPLC procedure based on the use of monolithic column.•The use of microwave assisted extraction for the isolation of the catechin compounds.•Application of the methods for the determination of catechins in various types of teas. A rapid reversed-phase high performance liquid c...
Gespeichert in:
| Veröffentlicht in: | Food chemistry 2014-03, Vol.147, p.262-268 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 268 |
|---|---|
| container_issue | |
| container_start_page | 262 |
| container_title | Food chemistry |
| container_volume | 147 |
| creator | Rahim, A.A. Nofrizal, S. Saad, Bahruddin |
| description | •Development of a rapid HPLC procedure based on the use of monolithic column.•The use of microwave assisted extraction for the isolation of the catechin compounds.•Application of the methods for the determination of catechins in various types of teas.
A rapid reversed-phase high performance liquid chromatographic method using a monolithic column for the determination of eight catechin monomers and caffeine was developed. Using a mobile phase of water:acetonitrile:methanol (83:6:11) at a flow rate of 1.4mLmin−1, the catechins and caffeine were isocratically separated in about 7min. The limits of detection and quantification were in the range of 0.11–0.29 and 0.33–0.87mgL−1, respectively. Satisfactory recoveries were obtained (94.2–105.2±1.8%) for all samples when spiked at three concentrations (5, 40 and 70mgL−1). In combination with microwave-assisted extraction (MAE), the method was applied to the determination of the catechins and caffeine in eleven tea samples (6 green, 3 black and 2 oolong teas). Relatively high levels of caffeine were found in black tea, but higher levels of the catechins, especially epigallocatechin gallate (EGCG) were found in green teas. |
| doi_str_mv | 10.1016/j.foodchem.2013.09.131 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1753511531</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0308814613013964</els_id><sourcerecordid>1753511531</sourcerecordid><originalsourceid>FETCH-LOGICAL-c530t-174be123cf7e676cfcdf42b68413cbc0f8552cd40ed7356227cbf676ba4174743</originalsourceid><addsrcrecordid>eNqF0U1v1DAQBuAIgehS-AuVL0hckvojsZ0baFUo0kogBGfLGY9ZrxJniZ2W_nuy3W057ska6ZnxaN6iuGK0YpTJ613lx9HBFoeKUyYq2lZMsBfFimklSkUVf1msqKC61KyWF8WblHaU0sXq18UFrzmVislVcf_D7oMjGS0BmxG2ISZio1sq7zFEJA4zTkOINocxku6B3H7frMmcQvxNhgDTeG_vsLQphZTREfybJwuP9jAmhT6AJcMYxz7kbQACYz8P8W3xyts-4bvTe1n8-nzzc31bbr59-br-tCmhETSXTNUdMi7AK5RKggfna95JXTMBHVCvm4aDqyk6JRrJuYLOL7Cz9dKqanFZfDjO3U_jnxlTNkNIgH1vI45zMkw1omGsEew8XTZqW1nL9jytG800V1wvVB7pcqmUJvRmP4XBTg-GUXOI0uzMU5TmEKWhrWGP61yd_pi7Ad1z21N2C3h_AjaB7f1kI4T032nBuW744j4eHS53vgs4mQQBI6ALE0I2bgzndvkHgOHAeQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1458182728</pqid></control><display><type>article</type><title>Rapid tea catechins and caffeine determination by HPLC using microwave-assisted extraction and silica monolithic column</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Rahim, A.A. ; Nofrizal, S. ; Saad, Bahruddin</creator><creatorcontrib>Rahim, A.A. ; Nofrizal, S. ; Saad, Bahruddin</creatorcontrib><description>•Development of a rapid HPLC procedure based on the use of monolithic column.•The use of microwave assisted extraction for the isolation of the catechin compounds.•Application of the methods for the determination of catechins in various types of teas.
A rapid reversed-phase high performance liquid chromatographic method using a monolithic column for the determination of eight catechin monomers and caffeine was developed. Using a mobile phase of water:acetonitrile:methanol (83:6:11) at a flow rate of 1.4mLmin−1, the catechins and caffeine were isocratically separated in about 7min. The limits of detection and quantification were in the range of 0.11–0.29 and 0.33–0.87mgL−1, respectively. Satisfactory recoveries were obtained (94.2–105.2±1.8%) for all samples when spiked at three concentrations (5, 40 and 70mgL−1). In combination with microwave-assisted extraction (MAE), the method was applied to the determination of the catechins and caffeine in eleven tea samples (6 green, 3 black and 2 oolong teas). Relatively high levels of caffeine were found in black tea, but higher levels of the catechins, especially epigallocatechin gallate (EGCG) were found in green teas.</description><identifier>ISSN: 0308-8146</identifier><identifier>EISSN: 1873-7072</identifier><identifier>DOI: 10.1016/j.foodchem.2013.09.131</identifier><identifier>PMID: 24206716</identifier><identifier>CODEN: FOCHDJ</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Biological and medical sciences ; Black tea ; Caffeine ; Caffeine - analysis ; Caffeine - isolation & purification ; Camellia sinensis - chemistry ; Catechin ; Catechin - analysis ; Catechin - isolation & purification ; Catechins ; Chemical Fractionation - instrumentation ; Chemical Fractionation - methods ; Chromatography, Reverse-Phase - methods ; Extraction ; Foods ; Green tea ; High performance liquid chromatography ; Liquids ; Medical sciences ; Microwaves ; Monolithic column ; Monomers ; Neuropharmacology ; Oolong tea ; Pharmacology. Drug treatments ; Plant Extracts - analysis ; Plant Extracts - isolation & purification ; Psychoanaleptics: cns stimulant, antidepressant agent, nootropic agent, mood stabilizer ; Psychoanaleptics: cns stimulant, antidepressant agent, nootropic agent, mood stabilizer..., (alzheimer disease) ; Psychology. Psychoanalysis. Psychiatry ; Psychopharmacology ; Tea</subject><ispartof>Food chemistry, 2014-03, Vol.147, p.262-268</ispartof><rights>2013 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2013 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c530t-174be123cf7e676cfcdf42b68413cbc0f8552cd40ed7356227cbf676ba4174743</citedby><cites>FETCH-LOGICAL-c530t-174be123cf7e676cfcdf42b68413cbc0f8552cd40ed7356227cbf676ba4174743</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0308814613013964$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28322852$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24206716$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rahim, A.A.</creatorcontrib><creatorcontrib>Nofrizal, S.</creatorcontrib><creatorcontrib>Saad, Bahruddin</creatorcontrib><title>Rapid tea catechins and caffeine determination by HPLC using microwave-assisted extraction and silica monolithic column</title><title>Food chemistry</title><addtitle>Food Chem</addtitle><description>•Development of a rapid HPLC procedure based on the use of monolithic column.•The use of microwave assisted extraction for the isolation of the catechin compounds.•Application of the methods for the determination of catechins in various types of teas.
A rapid reversed-phase high performance liquid chromatographic method using a monolithic column for the determination of eight catechin monomers and caffeine was developed. Using a mobile phase of water:acetonitrile:methanol (83:6:11) at a flow rate of 1.4mLmin−1, the catechins and caffeine were isocratically separated in about 7min. The limits of detection and quantification were in the range of 0.11–0.29 and 0.33–0.87mgL−1, respectively. Satisfactory recoveries were obtained (94.2–105.2±1.8%) for all samples when spiked at three concentrations (5, 40 and 70mgL−1). In combination with microwave-assisted extraction (MAE), the method was applied to the determination of the catechins and caffeine in eleven tea samples (6 green, 3 black and 2 oolong teas). Relatively high levels of caffeine were found in black tea, but higher levels of the catechins, especially epigallocatechin gallate (EGCG) were found in green teas.</description><subject>Biological and medical sciences</subject><subject>Black tea</subject><subject>Caffeine</subject><subject>Caffeine - analysis</subject><subject>Caffeine - isolation & purification</subject><subject>Camellia sinensis - chemistry</subject><subject>Catechin</subject><subject>Catechin - analysis</subject><subject>Catechin - isolation & purification</subject><subject>Catechins</subject><subject>Chemical Fractionation - instrumentation</subject><subject>Chemical Fractionation - methods</subject><subject>Chromatography, Reverse-Phase - methods</subject><subject>Extraction</subject><subject>Foods</subject><subject>Green tea</subject><subject>High performance liquid chromatography</subject><subject>Liquids</subject><subject>Medical sciences</subject><subject>Microwaves</subject><subject>Monolithic column</subject><subject>Monomers</subject><subject>Neuropharmacology</subject><subject>Oolong tea</subject><subject>Pharmacology. Drug treatments</subject><subject>Plant Extracts - analysis</subject><subject>Plant Extracts - isolation & purification</subject><subject>Psychoanaleptics: cns stimulant, antidepressant agent, nootropic agent, mood stabilizer</subject><subject>Psychoanaleptics: cns stimulant, antidepressant agent, nootropic agent, mood stabilizer..., (alzheimer disease)</subject><subject>Psychology. Psychoanalysis. Psychiatry</subject><subject>Psychopharmacology</subject><subject>Tea</subject><issn>0308-8146</issn><issn>1873-7072</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U1v1DAQBuAIgehS-AuVL0hckvojsZ0baFUo0kogBGfLGY9ZrxJniZ2W_nuy3W057ska6ZnxaN6iuGK0YpTJ613lx9HBFoeKUyYq2lZMsBfFimklSkUVf1msqKC61KyWF8WblHaU0sXq18UFrzmVislVcf_D7oMjGS0BmxG2ISZio1sq7zFEJA4zTkOINocxku6B3H7frMmcQvxNhgDTeG_vsLQphZTREfybJwuP9jAmhT6AJcMYxz7kbQACYz8P8W3xyts-4bvTe1n8-nzzc31bbr59-br-tCmhETSXTNUdMi7AK5RKggfna95JXTMBHVCvm4aDqyk6JRrJuYLOL7Cz9dKqanFZfDjO3U_jnxlTNkNIgH1vI45zMkw1omGsEew8XTZqW1nL9jytG800V1wvVB7pcqmUJvRmP4XBTg-GUXOI0uzMU5TmEKWhrWGP61yd_pi7Ad1z21N2C3h_AjaB7f1kI4T032nBuW744j4eHS53vgs4mQQBI6ALE0I2bgzndvkHgOHAeQ</recordid><startdate>20140315</startdate><enddate>20140315</enddate><creator>Rahim, A.A.</creator><creator>Nofrizal, S.</creator><creator>Saad, Bahruddin</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U7</scope><scope>C1K</scope><scope>7QQ</scope><scope>7U5</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20140315</creationdate><title>Rapid tea catechins and caffeine determination by HPLC using microwave-assisted extraction and silica monolithic column</title><author>Rahim, A.A. ; Nofrizal, S. ; Saad, Bahruddin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c530t-174be123cf7e676cfcdf42b68413cbc0f8552cd40ed7356227cbf676ba4174743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Biological and medical sciences</topic><topic>Black tea</topic><topic>Caffeine</topic><topic>Caffeine - analysis</topic><topic>Caffeine - isolation & purification</topic><topic>Camellia sinensis - chemistry</topic><topic>Catechin</topic><topic>Catechin - analysis</topic><topic>Catechin - isolation & purification</topic><topic>Catechins</topic><topic>Chemical Fractionation - instrumentation</topic><topic>Chemical Fractionation - methods</topic><topic>Chromatography, Reverse-Phase - methods</topic><topic>Extraction</topic><topic>Foods</topic><topic>Green tea</topic><topic>High performance liquid chromatography</topic><topic>Liquids</topic><topic>Medical sciences</topic><topic>Microwaves</topic><topic>Monolithic column</topic><topic>Monomers</topic><topic>Neuropharmacology</topic><topic>Oolong tea</topic><topic>Pharmacology. Drug treatments</topic><topic>Plant Extracts - analysis</topic><topic>Plant Extracts - isolation & purification</topic><topic>Psychoanaleptics: cns stimulant, antidepressant agent, nootropic agent, mood stabilizer</topic><topic>Psychoanaleptics: cns stimulant, antidepressant agent, nootropic agent, mood stabilizer..., (alzheimer disease)</topic><topic>Psychology. Psychoanalysis. Psychiatry</topic><topic>Psychopharmacology</topic><topic>Tea</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rahim, A.A.</creatorcontrib><creatorcontrib>Nofrizal, S.</creatorcontrib><creatorcontrib>Saad, Bahruddin</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Ceramic Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rahim, A.A.</au><au>Nofrizal, S.</au><au>Saad, Bahruddin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid tea catechins and caffeine determination by HPLC using microwave-assisted extraction and silica monolithic column</atitle><jtitle>Food chemistry</jtitle><addtitle>Food Chem</addtitle><date>2014-03-15</date><risdate>2014</risdate><volume>147</volume><spage>262</spage><epage>268</epage><pages>262-268</pages><issn>0308-8146</issn><eissn>1873-7072</eissn><coden>FOCHDJ</coden><abstract>•Development of a rapid HPLC procedure based on the use of monolithic column.•The use of microwave assisted extraction for the isolation of the catechin compounds.•Application of the methods for the determination of catechins in various types of teas.
A rapid reversed-phase high performance liquid chromatographic method using a monolithic column for the determination of eight catechin monomers and caffeine was developed. Using a mobile phase of water:acetonitrile:methanol (83:6:11) at a flow rate of 1.4mLmin−1, the catechins and caffeine were isocratically separated in about 7min. The limits of detection and quantification were in the range of 0.11–0.29 and 0.33–0.87mgL−1, respectively. Satisfactory recoveries were obtained (94.2–105.2±1.8%) for all samples when spiked at three concentrations (5, 40 and 70mgL−1). In combination with microwave-assisted extraction (MAE), the method was applied to the determination of the catechins and caffeine in eleven tea samples (6 green, 3 black and 2 oolong teas). Relatively high levels of caffeine were found in black tea, but higher levels of the catechins, especially epigallocatechin gallate (EGCG) were found in green teas.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>24206716</pmid><doi>10.1016/j.foodchem.2013.09.131</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0308-8146 |
| ispartof | Food chemistry, 2014-03, Vol.147, p.262-268 |
| issn | 0308-8146 1873-7072 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1753511531 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Biological and medical sciences Black tea Caffeine Caffeine - analysis Caffeine - isolation & purification Camellia sinensis - chemistry Catechin Catechin - analysis Catechin - isolation & purification Catechins Chemical Fractionation - instrumentation Chemical Fractionation - methods Chromatography, Reverse-Phase - methods Extraction Foods Green tea High performance liquid chromatography Liquids Medical sciences Microwaves Monolithic column Monomers Neuropharmacology Oolong tea Pharmacology. Drug treatments Plant Extracts - analysis Plant Extracts - isolation & purification Psychoanaleptics: cns stimulant, antidepressant agent, nootropic agent, mood stabilizer Psychoanaleptics: cns stimulant, antidepressant agent, nootropic agent, mood stabilizer..., (alzheimer disease) Psychology. Psychoanalysis. Psychiatry Psychopharmacology Tea |
| title | Rapid tea catechins and caffeine determination by HPLC using microwave-assisted extraction and silica monolithic column |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T10%3A28%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Rapid%20tea%20catechins%20and%20caffeine%20determination%20by%20HPLC%20using%20microwave-assisted%20extraction%20and%20silica%20monolithic%20column&rft.jtitle=Food%20chemistry&rft.au=Rahim,%20A.A.&rft.date=2014-03-15&rft.volume=147&rft.spage=262&rft.epage=268&rft.pages=262-268&rft.issn=0308-8146&rft.eissn=1873-7072&rft.coden=FOCHDJ&rft_id=info:doi/10.1016/j.foodchem.2013.09.131&rft_dat=%3Cproquest_cross%3E1753511531%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1458182728&rft_id=info:pmid/24206716&rft_els_id=S0308814613013964&rfr_iscdi=true |