Near infrared Raman spectroscopic characterization of blood plasma of normal, oral premalignant and malignant conditions-a pilot study

The metabolic end products from cells/tissues that are released into the circulating blood stream and any changes in their level because of pathological conditions may be used as markers in disease diagnosis. Raman spectroscopy has been exploited to characterize the biomolecules present in the blood...

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Veröffentlicht in:Journal of Raman spectroscopy 2015-09, Vol.46 (9), p.735-743
Hauptverfasser: Rekha, Pachaiappan, Aruna, Prakasarao, Bharanidharan, Ganesan, Koteeswaran, Dornadula, Baludavid, Munusamy, Ganesan, Singaravelu
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container_end_page 743
container_issue 9
container_start_page 735
container_title Journal of Raman spectroscopy
container_volume 46
creator Rekha, Pachaiappan
Aruna, Prakasarao
Bharanidharan, Ganesan
Koteeswaran, Dornadula
Baludavid, Munusamy
Ganesan, Singaravelu
description The metabolic end products from cells/tissues that are released into the circulating blood stream and any changes in their level because of pathological conditions may be used as markers in disease diagnosis. Raman spectroscopy has been exploited to characterize the biomolecules present in the blood plasma of clinically confirmed normal group, premalignant (Oral Sub Mucous Fibrosis) and malignant (Oral Squamous Cell Carcinoma) at 784.15 nm. Raman spectral signatures show relatively less intense Raman bands of phenylalanine, lipid and antioxidant beta carotene but higher intense bands for proteins, DNA base components and amino acids (tyrosine and tryptophan) for malignant group than that of normal group. However premalignant group possess high intense Raman bands for amino acids (tyrosine and tryptophan) at 830, 1020 and 1620 cm−1 and protein peaks at 913, 978 and 1646 cm−1 when compared to that of malignant and normal group. Principal component analysis coupled with linear discriminant analysis (PCA‐LDA) yielded a diagnostic sensitivity of 96.3% and 91.2%, and a specificity of 80.0% and 96.7% in the classification of normal from premalignant and normal from malignant, respectively. This indicates that Raman spectroscopy of blood plasma has the potential in classifying normal and oral malignancy conditions. Copyright © 2015 John Wiley & Sons, Ltd. In this study, Raman spectroscopy has been exploited to characterize the biomolecules present in the blood plasma of normal group, premalignant (Oral Sub Mucous Fibrosis) and malignant (Oral Squamous Cell Carcinoma) at 784.15 nm. Raman bands of phenylalanine, lipid, antioxidant beta carotene, DNA base components and amino acids (tyrosine and trytophan) played a major role in the discrimination between the groups. The classification results of PCA‐LDA for normal versus abnormal group yielded an efficiency of 78%.
doi_str_mv 10.1002/jrs.4693
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Raman spectroscopy has been exploited to characterize the biomolecules present in the blood plasma of clinically confirmed normal group, premalignant (Oral Sub Mucous Fibrosis) and malignant (Oral Squamous Cell Carcinoma) at 784.15 nm. Raman spectral signatures show relatively less intense Raman bands of phenylalanine, lipid and antioxidant beta carotene but higher intense bands for proteins, DNA base components and amino acids (tyrosine and tryptophan) for malignant group than that of normal group. However premalignant group possess high intense Raman bands for amino acids (tyrosine and tryptophan) at 830, 1020 and 1620 cm−1 and protein peaks at 913, 978 and 1646 cm−1 when compared to that of malignant and normal group. Principal component analysis coupled with linear discriminant analysis (PCA‐LDA) yielded a diagnostic sensitivity of 96.3% and 91.2%, and a specificity of 80.0% and 96.7% in the classification of normal from premalignant and normal from malignant, respectively. This indicates that Raman spectroscopy of blood plasma has the potential in classifying normal and oral malignancy conditions. Copyright © 2015 John Wiley &amp; Sons, Ltd. In this study, Raman spectroscopy has been exploited to characterize the biomolecules present in the blood plasma of normal group, premalignant (Oral Sub Mucous Fibrosis) and malignant (Oral Squamous Cell Carcinoma) at 784.15 nm. Raman bands of phenylalanine, lipid, antioxidant beta carotene, DNA base components and amino acids (tyrosine and trytophan) played a major role in the discrimination between the groups. 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However premalignant group possess high intense Raman bands for amino acids (tyrosine and tryptophan) at 830, 1020 and 1620 cm−1 and protein peaks at 913, 978 and 1646 cm−1 when compared to that of malignant and normal group. Principal component analysis coupled with linear discriminant analysis (PCA‐LDA) yielded a diagnostic sensitivity of 96.3% and 91.2%, and a specificity of 80.0% and 96.7% in the classification of normal from premalignant and normal from malignant, respectively. This indicates that Raman spectroscopy of blood plasma has the potential in classifying normal and oral malignancy conditions. Copyright © 2015 John Wiley &amp; Sons, Ltd. In this study, Raman spectroscopy has been exploited to characterize the biomolecules present in the blood plasma of normal group, premalignant (Oral Sub Mucous Fibrosis) and malignant (Oral Squamous Cell Carcinoma) at 784.15 nm. 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source Wiley Journals
subjects Amino acids
Bands
betacarotene
Biomolecules
Blood plasma
cancer diagnosis
Carotene
Classification
guanine
principal component-linear discriminant analysis
Raman spectroscopy
Tryptophan
Tyrosine
title Near infrared Raman spectroscopic characterization of blood plasma of normal, oral premalignant and malignant conditions-a pilot study
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