Expression of the long-chain fatty acid receptor GPR120 in the gonadotropes of the mouse anterior pituitary gland
G-protein-coupled receptor 120 (GPR120) has been known to be a receptor of long-chain fatty acids. Here, we investigated GPR120 expression in the mouse pituitary gland via real-time PCR, in situ hybridization, and immunohistochemistry. GPR120 mRNA was abundantly expressed in the pituitary gland of a...
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| Veröffentlicht in: | Histochemistry and cell biology 2015-01, Vol.143 (1), p.21-27 |
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| creator | Moriyama, Ryutaro Deura, Chikaya Imoto, Shingo Nose, Kazuhiro Fukushima, Nobuyuki |
| description | G-protein-coupled receptor 120 (GPR120) has been known to be a receptor of long-chain fatty acids. Here, we investigated GPR120 expression in the mouse pituitary gland via real-time PCR, in situ hybridization, and immunohistochemistry. GPR120 mRNA was abundantly expressed in the pituitary gland of ad-lib fed animals. In situ hybridization and immunohistochemistry revealed GPR120 expression in the gonadotropes of the anterior pituitary gland, but not in thyrotropes, somatotropes, lactotropes, corticotropes, melanotropes, and the posterior pituitary gland. Furthermore, 24 h of fasting induced an increase in GPR120 mRNA expression in the pituitary gland. These results demonstrate that GPR120 in mouse pituitary gonadotropes is upregulated by fasting and that it may play a role in controlling gonadotropin secretion. |
| doi_str_mv | 10.1007/s00418-014-1257-3 |
| format | Article |
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Here, we investigated GPR120 expression in the mouse pituitary gland via real-time PCR, in situ hybridization, and immunohistochemistry. GPR120 mRNA was abundantly expressed in the pituitary gland of ad-lib fed animals. In situ hybridization and immunohistochemistry revealed GPR120 expression in the gonadotropes of the anterior pituitary gland, but not in thyrotropes, somatotropes, lactotropes, corticotropes, melanotropes, and the posterior pituitary gland. Furthermore, 24 h of fasting induced an increase in GPR120 mRNA expression in the pituitary gland. 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Here, we investigated GPR120 expression in the mouse pituitary gland via real-time PCR, in situ hybridization, and immunohistochemistry. GPR120 mRNA was abundantly expressed in the pituitary gland of ad-lib fed animals. In situ hybridization and immunohistochemistry revealed GPR120 expression in the gonadotropes of the anterior pituitary gland, but not in thyrotropes, somatotropes, lactotropes, corticotropes, melanotropes, and the posterior pituitary gland. Furthermore, 24 h of fasting induced an increase in GPR120 mRNA expression in the pituitary gland. These results demonstrate that GPR120 in mouse pituitary gonadotropes is upregulated by fasting and that it may play a role in controlling gonadotropin secretion.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>25112963</pmid><doi>10.1007/s00418-014-1257-3</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Biochemistry Biomedical and Life Sciences Biomedicine Cell Biology Developmental Biology Fatty acids Gene expression Gonadotrophs - cytology Gonadotrophs - metabolism Immunohistochemistry In Situ Hybridization Male Mice Mice, Inbred ICR Neurons Original Paper Pituitary gland Real-Time Polymerase Chain Reaction Receptors, G-Protein-Coupled - biosynthesis Receptors, G-Protein-Coupled - genetics RNA, Messenger - biosynthesis RNA, Messenger - genetics |
| title | Expression of the long-chain fatty acid receptor GPR120 in the gonadotropes of the mouse anterior pituitary gland |
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