ROLE OF LRP5 GENE IN THE OCCURENCE OF TYPE 2 DIABETES MELLITUS IN OFFSRINGS OF PATIENTS WITH TYPE 2 DIABETES MELLITUS

Aim: The aim of the present study was to examine whether low-density lipoprotein receptor related protein 5 gene (LRP5) variants are associated with Type 2 Diabetes Mellitus in Indian population. Background: LRP5 gene is a member of LDL receptor family consisting of 23 exons and spacing 136.6 Kb locates on 11q13.4chromosome.LRP5is essential for normal cholesterol metabolism and glucose-induced insulin secretion. The study was approved by the institute ethics committee as per ICMR guidelines. Methods: Twenty-seven subjects with family history of Type 2 Diabetes Mellitus volunteered for the study and twenty-seven matched controls without family history of Type 2 Diabetes Mellitus were enrolled as controls for this study. 3 ml whole blood was taken and assayed.DNA was extracted from peripheral blood by phenol-chloroform method.All coding exons of LRP5 were amplified by Polymerase Chain Reaction (PCR) using specific primers. Results: All 23 exons of LRP5 gene were sequenced from patient's DNA sample. A silent, point mutation was observed in exon 8 of the LRP5 gene in cases whereas no such polymorphism was observed in controls. Other exons showed no such mutations. Conclusion: Various observations indicate that LRP5 is a potential Type 2 Diabetes Mellitus susceptibility gene. The pathogeneses of Type 2 Diabetes Mellitus is complex and influenced by genetic and environmental factors. Multiple genes have been found to be associated with Type 2 Diabetes Mellitus. LRP5 gene may have an effect in the occurrence of Type 2 Diabetes Mellitus