Analysis of type 2 metallothionein gene from mangrove species (Kandelia candel)
Metallothioneins are Cys-rich, low-molecular weight, metal-binding proteins. In this paper, we aimed to evaluate the roles of type 2 metallothionein in Kandelia candel (KcMT2) by analyzing its mRNA expression in leaves in response to heavy metals, as well as the metal-binding properties of its prote...
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| Veröffentlicht in: | Trees (Berlin, West) West), 2012-10, Vol.26 (5), p.1537-1544 |
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| creator | Huang, Guo-Yong Wang, You-Shao Ying, Guang-Guo Dang, Ai-Cui |
| description | Metallothioneins are Cys-rich, low-molecular weight, metal-binding proteins. In this paper, we aimed to evaluate the roles of type 2 metallothionein in Kandelia candel (KcMT2) by analyzing its mRNA expression in leaves in response to heavy metals, as well as the metal-binding properties of its protein. The transcriptional response of KcMT2 gene to different heavy metals (Zn, Cu, Pb and Cd) was measured using real-time quantitative PCR. The results showed that the gene was regulated by the four metals tested. Significant increase in the expression of KcMT2 mRNA was also found in response to Zn, Cu, Pb and Cd. To analyze further the possible roles of KcMT2 against heavy metals, it was expressed in Escherichia coli BL21 as a carboxy-terminal extension of glutathione-S-transferase (GST). The E. coli cells expressing GST-KcMT2 fusion protein showed higher growth compared to the control cells under Zn, Cu, Pb or Cd exposure, and the GST-KcMT2 fusion protein had a stronger binding affinity for Zn, Cu, Pb and Cd than GST alone. Results presented here reveal that KcMT2 may play important roles in the heavy metal homeostasis or detoxification. |
| doi_str_mv | 10.1007/s00468-012-0727-2 |
| format | Article |
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In this paper, we aimed to evaluate the roles of type 2 metallothionein in Kandelia candel (KcMT2) by analyzing its mRNA expression in leaves in response to heavy metals, as well as the metal-binding properties of its protein. The transcriptional response of KcMT2 gene to different heavy metals (Zn, Cu, Pb and Cd) was measured using real-time quantitative PCR. The results showed that the gene was regulated by the four metals tested. Significant increase in the expression of KcMT2 mRNA was also found in response to Zn, Cu, Pb and Cd. To analyze further the possible roles of KcMT2 against heavy metals, it was expressed in Escherichia coli BL21 as a carboxy-terminal extension of glutathione-S-transferase (GST). The E. coli cells expressing GST-KcMT2 fusion protein showed higher growth compared to the control cells under Zn, Cu, Pb or Cd exposure, and the GST-KcMT2 fusion protein had a stronger binding affinity for Zn, Cu, Pb and Cd than GST alone. Results presented here reveal that KcMT2 may play important roles in the heavy metal homeostasis or detoxification.</description><identifier>ISSN: 0931-1890</identifier><identifier>EISSN: 1432-2285</identifier><identifier>DOI: 10.1007/s00468-012-0727-2</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Agriculture ; Biomedical and Life Sciences ; Cadmium ; Copper ; Detoxification ; E coli ; Escherichia coli ; Forestry ; gene expression ; genes ; Heavy metals ; homeostasis ; Kandelia candel ; Lead ; leaves ; Life Sciences ; messenger RNA ; metallothionein ; Metallothioneins ; Original Paper ; Plant Anatomy/Development ; Plant Pathology ; Plant Physiology ; Plant Sciences ; polymerase chain reaction ; Zinc</subject><ispartof>Trees (Berlin, West), 2012-10, Vol.26 (5), p.1537-1544</ispartof><rights>Springer-Verlag 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c373t-cb26c74ff5c4f5059f758893c1fdf3359717933955525670ba0470cbc91033183</citedby><cites>FETCH-LOGICAL-c373t-cb26c74ff5c4f5059f758893c1fdf3359717933955525670ba0470cbc91033183</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00468-012-0727-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00468-012-0727-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids></links><search><creatorcontrib>Huang, Guo-Yong</creatorcontrib><creatorcontrib>Wang, You-Shao</creatorcontrib><creatorcontrib>Ying, Guang-Guo</creatorcontrib><creatorcontrib>Dang, Ai-Cui</creatorcontrib><title>Analysis of type 2 metallothionein gene from mangrove species (Kandelia candel)</title><title>Trees (Berlin, West)</title><addtitle>Trees</addtitle><description>Metallothioneins are Cys-rich, low-molecular weight, metal-binding proteins. In this paper, we aimed to evaluate the roles of type 2 metallothionein in Kandelia candel (KcMT2) by analyzing its mRNA expression in leaves in response to heavy metals, as well as the metal-binding properties of its protein. The transcriptional response of KcMT2 gene to different heavy metals (Zn, Cu, Pb and Cd) was measured using real-time quantitative PCR. The results showed that the gene was regulated by the four metals tested. Significant increase in the expression of KcMT2 mRNA was also found in response to Zn, Cu, Pb and Cd. To analyze further the possible roles of KcMT2 against heavy metals, it was expressed in Escherichia coli BL21 as a carboxy-terminal extension of glutathione-S-transferase (GST). The E. coli cells expressing GST-KcMT2 fusion protein showed higher growth compared to the control cells under Zn, Cu, Pb or Cd exposure, and the GST-KcMT2 fusion protein had a stronger binding affinity for Zn, Cu, Pb and Cd than GST alone. Results presented here reveal that KcMT2 may play important roles in the heavy metal homeostasis or detoxification.</description><subject>Agriculture</subject><subject>Biomedical and Life Sciences</subject><subject>Cadmium</subject><subject>Copper</subject><subject>Detoxification</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Forestry</subject><subject>gene expression</subject><subject>genes</subject><subject>Heavy metals</subject><subject>homeostasis</subject><subject>Kandelia candel</subject><subject>Lead</subject><subject>leaves</subject><subject>Life Sciences</subject><subject>messenger RNA</subject><subject>metallothionein</subject><subject>Metallothioneins</subject><subject>Original Paper</subject><subject>Plant Anatomy/Development</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>polymerase chain reaction</subject><subject>Zinc</subject><issn>0931-1890</issn><issn>1432-2285</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kcFKJDEQhoO44Oj6AJ4MeHEP7VZSySQ5iugqCh52PYdMJhlbujtj0iPM22_G9iCCp6rD91dRXxFywuCCAajfBUDMdQOMN6C4avgemTGBvOFcy30yA4OsYdrAATks5QUAcM74jDxeDq7blrbQFOm4XQfKaR9G13VpfG7TENqBrsIQaMypp70bVjm9BVrWwbeh0PN7NyxD1zrq35tfP8mP6LoSjj_qEXm6uf53dds8PP65u7p8aDwqHBu_4HOvRIzSiyhBmqik1gY9i8uIKI1iyiAaKSWXcwULB0KBX3jDAJFpPCLn09x1Tq-bUEbbt8WHrnNDSJtimZIolNCIFT37gr6kTa5nVwqwbkEtoFJsonxOpeQQ7Tq3vcvbCtmdYjsptlWx3Sm2vGb4lCmVHVYhf578feh0CkWXrFvlttinvxyYqD8RxhiN_wEsA4TW</recordid><startdate>20121001</startdate><enddate>20121001</enddate><creator>Huang, Guo-Yong</creator><creator>Wang, You-Shao</creator><creator>Ying, Guang-Guo</creator><creator>Dang, Ai-Cui</creator><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7ST</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>SOI</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20121001</creationdate><title>Analysis of type 2 metallothionein gene from mangrove species (Kandelia candel)</title><author>Huang, Guo-Yong ; 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In this paper, we aimed to evaluate the roles of type 2 metallothionein in Kandelia candel (KcMT2) by analyzing its mRNA expression in leaves in response to heavy metals, as well as the metal-binding properties of its protein. The transcriptional response of KcMT2 gene to different heavy metals (Zn, Cu, Pb and Cd) was measured using real-time quantitative PCR. The results showed that the gene was regulated by the four metals tested. Significant increase in the expression of KcMT2 mRNA was also found in response to Zn, Cu, Pb and Cd. To analyze further the possible roles of KcMT2 against heavy metals, it was expressed in Escherichia coli BL21 as a carboxy-terminal extension of glutathione-S-transferase (GST). The E. coli cells expressing GST-KcMT2 fusion protein showed higher growth compared to the control cells under Zn, Cu, Pb or Cd exposure, and the GST-KcMT2 fusion protein had a stronger binding affinity for Zn, Cu, Pb and Cd than GST alone. Results presented here reveal that KcMT2 may play important roles in the heavy metal homeostasis or detoxification.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><doi>10.1007/s00468-012-0727-2</doi><tpages>8</tpages></addata></record> |
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| subjects | Agriculture Biomedical and Life Sciences Cadmium Copper Detoxification E coli Escherichia coli Forestry gene expression genes Heavy metals homeostasis Kandelia candel Lead leaves Life Sciences messenger RNA metallothionein Metallothioneins Original Paper Plant Anatomy/Development Plant Pathology Plant Physiology Plant Sciences polymerase chain reaction Zinc |
| title | Analysis of type 2 metallothionein gene from mangrove species (Kandelia candel) |
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