Evidence for Vitamin D Receptor Expression and Direct Effects of 1α,25(OH)2D3 in Human Skeletal Muscle Precursor Cells
Presence of the vitamin D receptor and direct effects of vitamin D on the proliferation and differentiation of muscle precursor cells have been demonstrated in animal models. However, the effects and mechanisms of vitamin D actions in human skeletal muscle, and the presence of the vitamin D receptor...
Gespeichert in:
| Veröffentlicht in: | Endocrinology (Philadelphia) 2016-01, Vol.157 (1), p.98-111 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 111 |
|---|---|
| container_issue | 1 |
| container_start_page | 98 |
| container_title | Endocrinology (Philadelphia) |
| container_volume | 157 |
| creator | Olsson, Karl Saini, Amarjit Strömberg, Anna Alam, Seher Lilja, Mats Rullman, Eric Gustafsson, Thomas |
| description | Presence of the vitamin D receptor and direct effects of vitamin D on the proliferation and differentiation of muscle precursor cells have been demonstrated in animal models. However, the effects and mechanisms of vitamin D actions in human skeletal muscle, and the presence of the vitamin D receptor in human adult skeletal muscle, remain to be established. Here, we investigated the role of vitamin D in human muscle cells at various stages of differentiation. We demonstrate that the components of the vitamin D-endocrine system are readily detected in human muscle precursor cells but are low to nondetectable in adult skeletal muscle and that human muscle cells lack the ability to convert the inactive vitamin D-metabolite 25-hydroxy-vitamin D3 to the active 1α,25-dihydroxy-vitamin D3 (1α,25(OH)2D3). In addition, we show that 1α,25(OH)2D3 inhibits myoblast proliferation and differentiation by altering the expression of cell cycle regulators and myogenic regulatory factors, with associated changes in forkhead box O3 and Notch signaling pathways. The present data add novel information regarding the direct effects of vitamin D in human skeletal muscle and provide functional and mechanistic insight to the regulation of myoblast cell fate decisions by 1α,25(OH)2D3. |
| doi_str_mv | 10.1210/en.2015-1685 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1753009655</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1210/en.2015-1685</oup_id><sourcerecordid>3130631612</sourcerecordid><originalsourceid>FETCH-LOGICAL-c433t-6eb9d2984cb93feef2d7e12ac62b88aa94dbf62d987452c46e40b85e409285da3</originalsourceid><addsrcrecordid>eNp1kcuKFDEUhoMoTju6cy0BF44wNeZelaV0t7YwMuJtG1LJKaixKimTKi-P5Yv4TKbtVkF0k0MO3_n44UfoPiUXlFHyBMIFI1RWVDXyBlpRLWRV05rcRCtCKK9qxuoTdCfn6_IVQvDb6IQpoTTl9Qp93n7qPQQHuIsJv-9nO_YBb_BrcDDNZbX9MiXIuY8B2-Dxpk_gZrztujIyjh2m37-dM3l2tXvMNhyX490y2oDffIABZjvgl0t2A-BX5W5JuRjXMAz5LrrV2SHDveM8Re-ebd-ud9Xl1fMX66eXlROcz5WCVnumG-FazTuAjvkaKLNOsbZprNXCt51iXje1kMwJBYK0jSyvZo30lp-is4N3SvHjAnk2Y59dSWADxCUbWktOiFZSFvThX-h1XFIo6QynnChOFWWFOj9QLsWcE3RmSv1o01dDidkXYiCYfSFmX0jBHxylSzuC_w3_aqAAjw5AXKb_qaqjih9ICD661Af42cyflP8M8ANnIqHI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3130631612</pqid></control><display><type>article</type><title>Evidence for Vitamin D Receptor Expression and Direct Effects of 1α,25(OH)2D3 in Human Skeletal Muscle Precursor Cells</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><source>Journals@Ovid Complete</source><creator>Olsson, Karl ; Saini, Amarjit ; Strömberg, Anna ; Alam, Seher ; Lilja, Mats ; Rullman, Eric ; Gustafsson, Thomas</creator><creatorcontrib>Olsson, Karl ; Saini, Amarjit ; Strömberg, Anna ; Alam, Seher ; Lilja, Mats ; Rullman, Eric ; Gustafsson, Thomas</creatorcontrib><description>Presence of the vitamin D receptor and direct effects of vitamin D on the proliferation and differentiation of muscle precursor cells have been demonstrated in animal models. However, the effects and mechanisms of vitamin D actions in human skeletal muscle, and the presence of the vitamin D receptor in human adult skeletal muscle, remain to be established. Here, we investigated the role of vitamin D in human muscle cells at various stages of differentiation. We demonstrate that the components of the vitamin D-endocrine system are readily detected in human muscle precursor cells but are low to nondetectable in adult skeletal muscle and that human muscle cells lack the ability to convert the inactive vitamin D-metabolite 25-hydroxy-vitamin D3 to the active 1α,25-dihydroxy-vitamin D3 (1α,25(OH)2D3). In addition, we show that 1α,25(OH)2D3 inhibits myoblast proliferation and differentiation by altering the expression of cell cycle regulators and myogenic regulatory factors, with associated changes in forkhead box O3 and Notch signaling pathways. The present data add novel information regarding the direct effects of vitamin D in human skeletal muscle and provide functional and mechanistic insight to the regulation of myoblast cell fate decisions by 1α,25(OH)2D3.</description><identifier>ISSN: 0013-7227</identifier><identifier>EISSN: 1945-7170</identifier><identifier>DOI: 10.1210/en.2015-1685</identifier><identifier>PMID: 26469137</identifier><language>eng</language><publisher>United States: Endocrine Society</publisher><subject>Adult ; Animal models ; Biopsy, Needle ; Calcifediol - metabolism ; Calciferol ; Calcitriol - metabolism ; Cell culture ; Cell cycle ; Cell differentiation ; Cell fate ; Cell Line ; Cell Proliferation ; Cells, Cultured ; Differentiation ; Endocrine system ; Female ; Forkhead Box Protein O3 ; Forkhead protein ; Forkhead Transcription Factors - genetics ; Forkhead Transcription Factors - metabolism ; Gene Expression Profiling ; Gene Expression Regulation, Developmental ; Human performance ; Humans ; Hydroxylation ; Male ; Metabolites ; Monocytes - metabolism ; Muscle Development ; Muscle Fibers, Skeletal - cytology ; Muscle Fibers, Skeletal - metabolism ; Muscle regulatory factor ; Muscles ; Musculoskeletal system ; Myoblasts ; Myoblasts, Skeletal - cytology ; Myoblasts, Skeletal - metabolism ; Precursors ; Receptors ; Receptors, Calcitriol - agonists ; Receptors, Calcitriol - genetics ; Receptors, Calcitriol - metabolism ; Receptors, Notch - genetics ; Receptors, Notch - metabolism ; Signal Transduction ; Skeletal muscle ; Vitamin D ; Vitamin D receptors ; Vitamin D3 ; Young Adult</subject><ispartof>Endocrinology (Philadelphia), 2016-01, Vol.157 (1), p.98-111</ispartof><rights>Copyright © 2016 by the Endocrine Society</rights><rights>Copyright © 2016 by the Endocrine Society 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c433t-6eb9d2984cb93feef2d7e12ac62b88aa94dbf62d987452c46e40b85e409285da3</citedby><cites>FETCH-LOGICAL-c433t-6eb9d2984cb93feef2d7e12ac62b88aa94dbf62d987452c46e40b85e409285da3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26469137$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Olsson, Karl</creatorcontrib><creatorcontrib>Saini, Amarjit</creatorcontrib><creatorcontrib>Strömberg, Anna</creatorcontrib><creatorcontrib>Alam, Seher</creatorcontrib><creatorcontrib>Lilja, Mats</creatorcontrib><creatorcontrib>Rullman, Eric</creatorcontrib><creatorcontrib>Gustafsson, Thomas</creatorcontrib><title>Evidence for Vitamin D Receptor Expression and Direct Effects of 1α,25(OH)2D3 in Human Skeletal Muscle Precursor Cells</title><title>Endocrinology (Philadelphia)</title><addtitle>Endocrinology</addtitle><description>Presence of the vitamin D receptor and direct effects of vitamin D on the proliferation and differentiation of muscle precursor cells have been demonstrated in animal models. However, the effects and mechanisms of vitamin D actions in human skeletal muscle, and the presence of the vitamin D receptor in human adult skeletal muscle, remain to be established. Here, we investigated the role of vitamin D in human muscle cells at various stages of differentiation. We demonstrate that the components of the vitamin D-endocrine system are readily detected in human muscle precursor cells but are low to nondetectable in adult skeletal muscle and that human muscle cells lack the ability to convert the inactive vitamin D-metabolite 25-hydroxy-vitamin D3 to the active 1α,25-dihydroxy-vitamin D3 (1α,25(OH)2D3). In addition, we show that 1α,25(OH)2D3 inhibits myoblast proliferation and differentiation by altering the expression of cell cycle regulators and myogenic regulatory factors, with associated changes in forkhead box O3 and Notch signaling pathways. The present data add novel information regarding the direct effects of vitamin D in human skeletal muscle and provide functional and mechanistic insight to the regulation of myoblast cell fate decisions by 1α,25(OH)2D3.</description><subject>Adult</subject><subject>Animal models</subject><subject>Biopsy, Needle</subject><subject>Calcifediol - metabolism</subject><subject>Calciferol</subject><subject>Calcitriol - metabolism</subject><subject>Cell culture</subject><subject>Cell cycle</subject><subject>Cell differentiation</subject><subject>Cell fate</subject><subject>Cell Line</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>Differentiation</subject><subject>Endocrine system</subject><subject>Female</subject><subject>Forkhead Box Protein O3</subject><subject>Forkhead protein</subject><subject>Forkhead Transcription Factors - genetics</subject><subject>Forkhead Transcription Factors - metabolism</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Human performance</subject><subject>Humans</subject><subject>Hydroxylation</subject><subject>Male</subject><subject>Metabolites</subject><subject>Monocytes - metabolism</subject><subject>Muscle Development</subject><subject>Muscle Fibers, Skeletal - cytology</subject><subject>Muscle Fibers, Skeletal - metabolism</subject><subject>Muscle regulatory factor</subject><subject>Muscles</subject><subject>Musculoskeletal system</subject><subject>Myoblasts</subject><subject>Myoblasts, Skeletal - cytology</subject><subject>Myoblasts, Skeletal - metabolism</subject><subject>Precursors</subject><subject>Receptors</subject><subject>Receptors, Calcitriol - agonists</subject><subject>Receptors, Calcitriol - genetics</subject><subject>Receptors, Calcitriol - metabolism</subject><subject>Receptors, Notch - genetics</subject><subject>Receptors, Notch - metabolism</subject><subject>Signal Transduction</subject><subject>Skeletal muscle</subject><subject>Vitamin D</subject><subject>Vitamin D receptors</subject><subject>Vitamin D3</subject><subject>Young Adult</subject><issn>0013-7227</issn><issn>1945-7170</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kcuKFDEUhoMoTju6cy0BF44wNeZelaV0t7YwMuJtG1LJKaixKimTKi-P5Yv4TKbtVkF0k0MO3_n44UfoPiUXlFHyBMIFI1RWVDXyBlpRLWRV05rcRCtCKK9qxuoTdCfn6_IVQvDb6IQpoTTl9Qp93n7qPQQHuIsJv-9nO_YBb_BrcDDNZbX9MiXIuY8B2-Dxpk_gZrztujIyjh2m37-dM3l2tXvMNhyX490y2oDffIABZjvgl0t2A-BX5W5JuRjXMAz5LrrV2SHDveM8Re-ebd-ud9Xl1fMX66eXlROcz5WCVnumG-FazTuAjvkaKLNOsbZprNXCt51iXje1kMwJBYK0jSyvZo30lp-is4N3SvHjAnk2Y59dSWADxCUbWktOiFZSFvThX-h1XFIo6QynnChOFWWFOj9QLsWcE3RmSv1o01dDidkXYiCYfSFmX0jBHxylSzuC_w3_aqAAjw5AXKb_qaqjih9ICD661Af42cyflP8M8ANnIqHI</recordid><startdate>201601</startdate><enddate>201601</enddate><creator>Olsson, Karl</creator><creator>Saini, Amarjit</creator><creator>Strömberg, Anna</creator><creator>Alam, Seher</creator><creator>Lilja, Mats</creator><creator>Rullman, Eric</creator><creator>Gustafsson, Thomas</creator><general>Endocrine Society</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201601</creationdate><title>Evidence for Vitamin D Receptor Expression and Direct Effects of 1α,25(OH)2D3 in Human Skeletal Muscle Precursor Cells</title><author>Olsson, Karl ; Saini, Amarjit ; Strömberg, Anna ; Alam, Seher ; Lilja, Mats ; Rullman, Eric ; Gustafsson, Thomas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c433t-6eb9d2984cb93feef2d7e12ac62b88aa94dbf62d987452c46e40b85e409285da3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adult</topic><topic>Animal models</topic><topic>Biopsy, Needle</topic><topic>Calcifediol - metabolism</topic><topic>Calciferol</topic><topic>Calcitriol - metabolism</topic><topic>Cell culture</topic><topic>Cell cycle</topic><topic>Cell differentiation</topic><topic>Cell fate</topic><topic>Cell Line</topic><topic>Cell Proliferation</topic><topic>Cells, Cultured</topic><topic>Differentiation</topic><topic>Endocrine system</topic><topic>Female</topic><topic>Forkhead Box Protein O3</topic><topic>Forkhead protein</topic><topic>Forkhead Transcription Factors - genetics</topic><topic>Forkhead Transcription Factors - metabolism</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Human performance</topic><topic>Humans</topic><topic>Hydroxylation</topic><topic>Male</topic><topic>Metabolites</topic><topic>Monocytes - metabolism</topic><topic>Muscle Development</topic><topic>Muscle Fibers, Skeletal - cytology</topic><topic>Muscle Fibers, Skeletal - metabolism</topic><topic>Muscle regulatory factor</topic><topic>Muscles</topic><topic>Musculoskeletal system</topic><topic>Myoblasts</topic><topic>Myoblasts, Skeletal - cytology</topic><topic>Myoblasts, Skeletal - metabolism</topic><topic>Precursors</topic><topic>Receptors</topic><topic>Receptors, Calcitriol - agonists</topic><topic>Receptors, Calcitriol - genetics</topic><topic>Receptors, Calcitriol - metabolism</topic><topic>Receptors, Notch - genetics</topic><topic>Receptors, Notch - metabolism</topic><topic>Signal Transduction</topic><topic>Skeletal muscle</topic><topic>Vitamin D</topic><topic>Vitamin D receptors</topic><topic>Vitamin D3</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Olsson, Karl</creatorcontrib><creatorcontrib>Saini, Amarjit</creatorcontrib><creatorcontrib>Strömberg, Anna</creatorcontrib><creatorcontrib>Alam, Seher</creatorcontrib><creatorcontrib>Lilja, Mats</creatorcontrib><creatorcontrib>Rullman, Eric</creatorcontrib><creatorcontrib>Gustafsson, Thomas</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Endocrinology (Philadelphia)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Olsson, Karl</au><au>Saini, Amarjit</au><au>Strömberg, Anna</au><au>Alam, Seher</au><au>Lilja, Mats</au><au>Rullman, Eric</au><au>Gustafsson, Thomas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evidence for Vitamin D Receptor Expression and Direct Effects of 1α,25(OH)2D3 in Human Skeletal Muscle Precursor Cells</atitle><jtitle>Endocrinology (Philadelphia)</jtitle><addtitle>Endocrinology</addtitle><date>2016-01</date><risdate>2016</risdate><volume>157</volume><issue>1</issue><spage>98</spage><epage>111</epage><pages>98-111</pages><issn>0013-7227</issn><eissn>1945-7170</eissn><abstract>Presence of the vitamin D receptor and direct effects of vitamin D on the proliferation and differentiation of muscle precursor cells have been demonstrated in animal models. However, the effects and mechanisms of vitamin D actions in human skeletal muscle, and the presence of the vitamin D receptor in human adult skeletal muscle, remain to be established. Here, we investigated the role of vitamin D in human muscle cells at various stages of differentiation. We demonstrate that the components of the vitamin D-endocrine system are readily detected in human muscle precursor cells but are low to nondetectable in adult skeletal muscle and that human muscle cells lack the ability to convert the inactive vitamin D-metabolite 25-hydroxy-vitamin D3 to the active 1α,25-dihydroxy-vitamin D3 (1α,25(OH)2D3). In addition, we show that 1α,25(OH)2D3 inhibits myoblast proliferation and differentiation by altering the expression of cell cycle regulators and myogenic regulatory factors, with associated changes in forkhead box O3 and Notch signaling pathways. The present data add novel information regarding the direct effects of vitamin D in human skeletal muscle and provide functional and mechanistic insight to the regulation of myoblast cell fate decisions by 1α,25(OH)2D3.</abstract><cop>United States</cop><pub>Endocrine Society</pub><pmid>26469137</pmid><doi>10.1210/en.2015-1685</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0013-7227 |
| ispartof | Endocrinology (Philadelphia), 2016-01, Vol.157 (1), p.98-111 |
| issn | 0013-7227 1945-7170 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1753009655 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection; Journals@Ovid Complete |
| subjects | Adult Animal models Biopsy, Needle Calcifediol - metabolism Calciferol Calcitriol - metabolism Cell culture Cell cycle Cell differentiation Cell fate Cell Line Cell Proliferation Cells, Cultured Differentiation Endocrine system Female Forkhead Box Protein O3 Forkhead protein Forkhead Transcription Factors - genetics Forkhead Transcription Factors - metabolism Gene Expression Profiling Gene Expression Regulation, Developmental Human performance Humans Hydroxylation Male Metabolites Monocytes - metabolism Muscle Development Muscle Fibers, Skeletal - cytology Muscle Fibers, Skeletal - metabolism Muscle regulatory factor Muscles Musculoskeletal system Myoblasts Myoblasts, Skeletal - cytology Myoblasts, Skeletal - metabolism Precursors Receptors Receptors, Calcitriol - agonists Receptors, Calcitriol - genetics Receptors, Calcitriol - metabolism Receptors, Notch - genetics Receptors, Notch - metabolism Signal Transduction Skeletal muscle Vitamin D Vitamin D receptors Vitamin D3 Young Adult |
| title | Evidence for Vitamin D Receptor Expression and Direct Effects of 1α,25(OH)2D3 in Human Skeletal Muscle Precursor Cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-20T11%3A43%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evidence%20for%20Vitamin%20D%20Receptor%20Expression%20and%20Direct%20Effects%20of%201%CE%B1,25(OH)2D3%20in%20Human%20Skeletal%20Muscle%20Precursor%20Cells&rft.jtitle=Endocrinology%20(Philadelphia)&rft.au=Olsson,%20Karl&rft.date=2016-01&rft.volume=157&rft.issue=1&rft.spage=98&rft.epage=111&rft.pages=98-111&rft.issn=0013-7227&rft.eissn=1945-7170&rft_id=info:doi/10.1210/en.2015-1685&rft_dat=%3Cproquest_cross%3E3130631612%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3130631612&rft_id=info:pmid/26469137&rft_oup_id=10.1210/en.2015-1685&rfr_iscdi=true |