Xanthine oxidoreductase activity assay in tissues using stable isotope-labeled substrate and liquid chromatography high-resolution mass spectrometry

•New XOR activity assay was developed using [15N2]-xanthine as substrate.•[15N2]-UA production was measured by LC/HRMS.•[15N2]-UA determination was validated with good linearity, accuracy, and precision.•HRMS full-scan mode was used to examine endogenous inhibitor effects.•XOR assay results showed g...

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Veröffentlicht in:	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2016-01, Vol.1008, p.189-197
Hauptverfasser:	Murase, Takayo, Nampei, Mai, Oka, Mitsuru, Ashizawa, Naoki, Matsumoto, Koji, Miyachi, Atsushi, Nakamura, Takashi
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Chromatography, Liquid - methods High-resolution mass spectrometry Isotope Labeling Mass Spectrometry - methods Mice, Inbred ICR Stable isotope-labeled substrate Xanthine Dehydrogenase - metabolism Xanthine oxidoreductase activity Xanthine oxidoreductase inhibitor
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