TRPC1 is required for survival and proliferation of cochlear spiral ganglion stem/progenitor cells
Abstract Objective The present studies were designed to test the hypothesis that canonical transient receptor potential channel 1 (TRPC1) is required for the proliferation of cochlear spiral ganglion stem/progenitor cells (SPCs). Methods and materials TRPC1 were detected and evaluated in postnatal d...
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| Veröffentlicht in: | International journal of pediatric otorhinolaryngology 2015-12, Vol.79 (12), p.2290-2294 |
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| container_title | International journal of pediatric otorhinolaryngology |
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| creator | Chen, Hsin-Chien Wang, Chih-Hung Shih, Cheng-Ping Chueh, Sheau-Huei Liu, Shu-Fan Chen, Hang-Kang Lin, Yi-Chun |
| description | Abstract Objective The present studies were designed to test the hypothesis that canonical transient receptor potential channel 1 (TRPC1) is required for the proliferation of cochlear spiral ganglion stem/progenitor cells (SPCs). Methods and materials TRPC1 were detected and evaluated in postnatal day 1 CBA/CaJ mice pups derived-cochlear spiral ganglion SPCs by reverse transcription-polymerase chain reaction, Western blot, immunocytochemistry, and calcium imaging. The cell viability and proliferation of the spiral ganglion SPCs following si-RNA mediated knockdown of TRPC1 or addition of TRPC channel blocker SKF9635 were compared to controls. Results In spiral ganglion SPCs, TRPC1 was found to be the most abundantly expressed TRPC subunit and shown to contribute to store-operated calcium entry. Silencing of TRPC1 or addition of TRPC channel blockers significantly decreased the rate of cell proliferation. Conclusion The results suggest that TRPC1 might serve as an essential molecule in regulating the proliferation of spiral ganglion SPCs. |
| doi_str_mv | 10.1016/j.ijporl.2015.10.027 |
| format | Article |
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Methods and materials TRPC1 were detected and evaluated in postnatal day 1 CBA/CaJ mice pups derived-cochlear spiral ganglion SPCs by reverse transcription-polymerase chain reaction, Western blot, immunocytochemistry, and calcium imaging. The cell viability and proliferation of the spiral ganglion SPCs following si-RNA mediated knockdown of TRPC1 or addition of TRPC channel blocker SKF9635 were compared to controls. Results In spiral ganglion SPCs, TRPC1 was found to be the most abundantly expressed TRPC subunit and shown to contribute to store-operated calcium entry. Silencing of TRPC1 or addition of TRPC channel blockers significantly decreased the rate of cell proliferation. Conclusion The results suggest that TRPC1 might serve as an essential molecule in regulating the proliferation of spiral ganglion SPCs.</description><identifier>ISSN: 0165-5876</identifier><identifier>EISSN: 1872-8464</identifier><identifier>DOI: 10.1016/j.ijporl.2015.10.027</identifier><identifier>PMID: 26531006</identifier><language>eng</language><publisher>Ireland: Elsevier B.V</publisher><subject>Animals ; Animals, Newborn ; Canonical transient receptor potential channel ; Cell Proliferation - physiology ; Cell Survival - physiology ; Cochlea ; Cochlea - cytology ; Gene Silencing ; Mice, Inbred CBA ; Otolaryngology ; Pediatrics ; Proliferation ; Reverse Transcriptase Polymerase Chain Reaction ; Spiral Ganglion - cytology ; Spiral ganglion neuron ; Stem Cells - physiology ; Stem/progenitor cell ; TRPC Cation Channels - genetics ; TRPC Cation Channels - physiology</subject><ispartof>International journal of pediatric otorhinolaryngology, 2015-12, Vol.79 (12), p.2290-2294</ispartof><rights>Elsevier Ireland Ltd</rights><rights>2015 Elsevier Ireland Ltd</rights><rights>Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-3c741d8b9e58b0f9306fd5ad769d5f665fc3dedf3ad3342be749d8cb24affba23</citedby><cites>FETCH-LOGICAL-c417t-3c741d8b9e58b0f9306fd5ad769d5f665fc3dedf3ad3342be749d8cb24affba23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0165587615005340$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26531006$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Hsin-Chien</creatorcontrib><creatorcontrib>Wang, Chih-Hung</creatorcontrib><creatorcontrib>Shih, Cheng-Ping</creatorcontrib><creatorcontrib>Chueh, Sheau-Huei</creatorcontrib><creatorcontrib>Liu, Shu-Fan</creatorcontrib><creatorcontrib>Chen, Hang-Kang</creatorcontrib><creatorcontrib>Lin, Yi-Chun</creatorcontrib><title>TRPC1 is required for survival and proliferation of cochlear spiral ganglion stem/progenitor cells</title><title>International journal of pediatric otorhinolaryngology</title><addtitle>Int J Pediatr Otorhinolaryngol</addtitle><description>Abstract Objective The present studies were designed to test the hypothesis that canonical transient receptor potential channel 1 (TRPC1) is required for the proliferation of cochlear spiral ganglion stem/progenitor cells (SPCs). Methods and materials TRPC1 were detected and evaluated in postnatal day 1 CBA/CaJ mice pups derived-cochlear spiral ganglion SPCs by reverse transcription-polymerase chain reaction, Western blot, immunocytochemistry, and calcium imaging. The cell viability and proliferation of the spiral ganglion SPCs following si-RNA mediated knockdown of TRPC1 or addition of TRPC channel blocker SKF9635 were compared to controls. Results In spiral ganglion SPCs, TRPC1 was found to be the most abundantly expressed TRPC subunit and shown to contribute to store-operated calcium entry. Silencing of TRPC1 or addition of TRPC channel blockers significantly decreased the rate of cell proliferation. Conclusion The results suggest that TRPC1 might serve as an essential molecule in regulating the proliferation of spiral ganglion SPCs.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Canonical transient receptor potential channel</subject><subject>Cell Proliferation - physiology</subject><subject>Cell Survival - physiology</subject><subject>Cochlea</subject><subject>Cochlea - cytology</subject><subject>Gene Silencing</subject><subject>Mice, Inbred CBA</subject><subject>Otolaryngology</subject><subject>Pediatrics</subject><subject>Proliferation</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Spiral Ganglion - cytology</subject><subject>Spiral ganglion neuron</subject><subject>Stem Cells - physiology</subject><subject>Stem/progenitor cell</subject><subject>TRPC Cation Channels - genetics</subject><subject>TRPC Cation Channels - physiology</subject><issn>0165-5876</issn><issn>1872-8464</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1rFDEYgIMo7Vr7D0Tm6GW2-c7sRZClaqGgtPUcMsmbNWN2sk1mFvrvzbC1By-eAm-e9-t5EXpP8JpgIq-GdRgOKcc1xUTU0BpT9QqtSKdo23HJX6NVxUQrOiXP0dtSBoyJwkKcoXMqBSMYyxXqH-5-bEkTSpPhcQ4ZXONTbsqcj-FoYmNG1xxyisFDNlNIY5N8Y5P9FcFU7BByhXZm3MXlr0ywv6r4DsYw1TIWYizv0BtvYoHL5_cC_fxy_bD91t5-_3qz_XzbWk7U1DKrOHFdvwHR9dhvGJbeCeOU3DjhpRTeMgfOM-MY47QHxTeusz3lxvveUHaBPp7q1gEeZyiT3oeyTGBGSHPRRAkqOsYIqSg_oTanUjJ4fchhb_KTJlgvdvWgT3b1YneJVrs17cNzh7nfg3tJ-quzAp9OANQ9jwGyLjbAaMFVs3bSLoX_dfi3gI1hDNbE3_AEZUhzHqtDTXShGuv75cLLgYnAWDCO2R8Mn6QX</recordid><startdate>20151201</startdate><enddate>20151201</enddate><creator>Chen, Hsin-Chien</creator><creator>Wang, Chih-Hung</creator><creator>Shih, Cheng-Ping</creator><creator>Chueh, Sheau-Huei</creator><creator>Liu, Shu-Fan</creator><creator>Chen, Hang-Kang</creator><creator>Lin, Yi-Chun</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20151201</creationdate><title>TRPC1 is required for survival and proliferation of cochlear spiral ganglion stem/progenitor cells</title><author>Chen, Hsin-Chien ; Wang, Chih-Hung ; Shih, Cheng-Ping ; Chueh, Sheau-Huei ; Liu, Shu-Fan ; Chen, Hang-Kang ; Lin, Yi-Chun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-3c741d8b9e58b0f9306fd5ad769d5f665fc3dedf3ad3342be749d8cb24affba23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Canonical transient receptor potential channel</topic><topic>Cell Proliferation - physiology</topic><topic>Cell Survival - physiology</topic><topic>Cochlea</topic><topic>Cochlea - cytology</topic><topic>Gene Silencing</topic><topic>Mice, Inbred CBA</topic><topic>Otolaryngology</topic><topic>Pediatrics</topic><topic>Proliferation</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Spiral Ganglion - cytology</topic><topic>Spiral ganglion neuron</topic><topic>Stem Cells - physiology</topic><topic>Stem/progenitor cell</topic><topic>TRPC Cation Channels - genetics</topic><topic>TRPC Cation Channels - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Hsin-Chien</creatorcontrib><creatorcontrib>Wang, Chih-Hung</creatorcontrib><creatorcontrib>Shih, Cheng-Ping</creatorcontrib><creatorcontrib>Chueh, Sheau-Huei</creatorcontrib><creatorcontrib>Liu, Shu-Fan</creatorcontrib><creatorcontrib>Chen, Hang-Kang</creatorcontrib><creatorcontrib>Lin, Yi-Chun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of pediatric otorhinolaryngology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Hsin-Chien</au><au>Wang, Chih-Hung</au><au>Shih, Cheng-Ping</au><au>Chueh, Sheau-Huei</au><au>Liu, Shu-Fan</au><au>Chen, Hang-Kang</au><au>Lin, Yi-Chun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>TRPC1 is required for survival and proliferation of cochlear spiral ganglion stem/progenitor cells</atitle><jtitle>International journal of pediatric otorhinolaryngology</jtitle><addtitle>Int J Pediatr Otorhinolaryngol</addtitle><date>2015-12-01</date><risdate>2015</risdate><volume>79</volume><issue>12</issue><spage>2290</spage><epage>2294</epage><pages>2290-2294</pages><issn>0165-5876</issn><eissn>1872-8464</eissn><abstract>Abstract Objective The present studies were designed to test the hypothesis that canonical transient receptor potential channel 1 (TRPC1) is required for the proliferation of cochlear spiral ganglion stem/progenitor cells (SPCs). Methods and materials TRPC1 were detected and evaluated in postnatal day 1 CBA/CaJ mice pups derived-cochlear spiral ganglion SPCs by reverse transcription-polymerase chain reaction, Western blot, immunocytochemistry, and calcium imaging. The cell viability and proliferation of the spiral ganglion SPCs following si-RNA mediated knockdown of TRPC1 or addition of TRPC channel blocker SKF9635 were compared to controls. Results In spiral ganglion SPCs, TRPC1 was found to be the most abundantly expressed TRPC subunit and shown to contribute to store-operated calcium entry. Silencing of TRPC1 or addition of TRPC channel blockers significantly decreased the rate of cell proliferation. Conclusion The results suggest that TRPC1 might serve as an essential molecule in regulating the proliferation of spiral ganglion SPCs.</abstract><cop>Ireland</cop><pub>Elsevier B.V</pub><pmid>26531006</pmid><doi>10.1016/j.ijporl.2015.10.027</doi><tpages>5</tpages></addata></record> |
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| subjects | Animals Animals, Newborn Canonical transient receptor potential channel Cell Proliferation - physiology Cell Survival - physiology Cochlea Cochlea - cytology Gene Silencing Mice, Inbred CBA Otolaryngology Pediatrics Proliferation Reverse Transcriptase Polymerase Chain Reaction Spiral Ganglion - cytology Spiral ganglion neuron Stem Cells - physiology Stem/progenitor cell TRPC Cation Channels - genetics TRPC Cation Channels - physiology |
| title | TRPC1 is required for survival and proliferation of cochlear spiral ganglion stem/progenitor cells |
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