Curcumin-containing diet inhibits diethylnitrosamine-induced murine hepatocarcinogenesis
Curcumin has been widely used as a spice and coloring agent in foods. Recently, curcumin was found to possess chemopreventive effects against skin cancer, forestomach cancer, colon cancer and oral cancer in mice. Clinical trials of curcumin for prevention of human cancers are currently ongoing. In t...
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| Veröffentlicht in: | Carcinogenesis (New York) 2000-02, Vol.21 (2), p.331-335 |
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| creator | Chuang, S.E. Kuo, M.L. Hsu, C.H. Chen, C.R. Lin, J.K. Lai, G.M. Hsieh, C.Y. Cheng, A.L. |
| description | Curcumin has been widely used as a spice and coloring agent in foods. Recently, curcumin was found to possess chemopreventive effects against skin cancer, forestomach cancer, colon cancer and oral cancer in mice. Clinical trials of curcumin for prevention of human cancers are currently ongoing. In this study, we examine the chemopreventive effect of curcumin on murine hepatocarcinogenesis. C3H/HeN mice were injected i.p. with N-diethylnitrosamine (DEN) at the age of 5 weeks. The curcumin group started eating 0.2% curcumin-containing diet 4 days before DEN injection until death. The mice were then serially killed at the scheduled times to examine the development of hepatocellular carcinoma (HCC) and changes in intermediate biological markers. At the age of 42 weeks, the curcumin group, as compared with the control group (DEN alone), had an 81% reduction in multiplicity (0.5 versus 2.57) and a 62% reduction in incidence (38 versus 100%) of development of HCC. A series of intermediate biological markers were examined by western blot. While hepatic tissues obtained from the DEN-treated mice showed a remarkable increase in the levels of p21ras, PCNA and CDC2 proteins, eating a curcumin-containing diet reversed the levels to normal values. These results indicate that curcumin effectively inhibits DEN-induced hepatocarcinogenesis in the mouse. The underlying mechanisms of the phenomenon and the feasibility of using curcumin in the chemoprevention of human HCC should be further explored. |
| doi_str_mv | 10.1093/carcin/21.2.331 |
| format | Article |
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Recently, curcumin was found to possess chemopreventive effects against skin cancer, forestomach cancer, colon cancer and oral cancer in mice. Clinical trials of curcumin for prevention of human cancers are currently ongoing. In this study, we examine the chemopreventive effect of curcumin on murine hepatocarcinogenesis. C3H/HeN mice were injected i.p. with N-diethylnitrosamine (DEN) at the age of 5 weeks. The curcumin group started eating 0.2% curcumin-containing diet 4 days before DEN injection until death. The mice were then serially killed at the scheduled times to examine the development of hepatocellular carcinoma (HCC) and changes in intermediate biological markers. At the age of 42 weeks, the curcumin group, as compared with the control group (DEN alone), had an 81% reduction in multiplicity (0.5 versus 2.57) and a 62% reduction in incidence (38 versus 100%) of development of HCC. A series of intermediate biological markers were examined by western blot. While hepatic tissues obtained from the DEN-treated mice showed a remarkable increase in the levels of p21ras, PCNA and CDC2 proteins, eating a curcumin-containing diet reversed the levels to normal values. These results indicate that curcumin effectively inhibits DEN-induced hepatocarcinogenesis in the mouse. The underlying mechanisms of the phenomenon and the feasibility of using curcumin in the chemoprevention of human HCC should be further explored.</description><identifier>ISSN: 0143-3334</identifier><identifier>ISSN: 1460-2180</identifier><identifier>EISSN: 1460-2180</identifier><identifier>DOI: 10.1093/carcin/21.2.331</identifier><identifier>PMID: 10657978</identifier><identifier>CODEN: CRNGDP</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>12-O-tetradecanoylphorbol-13-acetate ; Animals ; Anti-Inflammatory Agents, Non-Steroidal - pharmacology ; Anti-Inflammatory Agents, Non-Steroidal - therapeutic use ; Anticarcinogenic Agents - therapeutic use ; Biological and medical sciences ; Biomarkers ; Carcinogenesis, carcinogens and anticarcinogens ; Carcinogens - toxicity ; CDC2 Protein Kinase - analysis ; Chemical and Drug Induced Liver Injury - prevention & control ; curcumin ; Curcumin - pharmacology ; Curcumin - therapeutic use ; DEN ; Diet ; Diethylnitrosamine - toxicity ; Foods and miscellaneous ; HCC ; hepatocellular carcinoma ; Liver - chemistry ; Liver - drug effects ; Liver Neoplasms, Experimental - chemically induced ; Liver Neoplasms, Experimental - prevention & control ; Male ; Medical sciences ; Mice ; Mice, Inbred C3H ; N-diethylnitrosamine ; Neoplasm Proteins - analysis ; PCNA ; PKC ; proliferating cell nuclear antigen ; Proliferating Cell Nuclear Antigen - analysis ; protein kinase C ; Proto-Oncogene Proteins p21(ras) - analysis ; Spices ; TPA ; Tumors</subject><ispartof>Carcinogenesis (New York), 2000-02, Vol.21 (2), p.331-335</ispartof><rights>2000 INIST-CNRS</rights><rights>Copyright Oxford University Press(England) Feb 2000</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c525t-78188a86ffc8b9ab843644b3a3a1997db9266561926af3eb204fc62f12fe553d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1339426$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10657978$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chuang, S.E.</creatorcontrib><creatorcontrib>Kuo, M.L.</creatorcontrib><creatorcontrib>Hsu, C.H.</creatorcontrib><creatorcontrib>Chen, C.R.</creatorcontrib><creatorcontrib>Lin, J.K.</creatorcontrib><creatorcontrib>Lai, G.M.</creatorcontrib><creatorcontrib>Hsieh, C.Y.</creatorcontrib><creatorcontrib>Cheng, A.L.</creatorcontrib><title>Curcumin-containing diet inhibits diethylnitrosamine-induced murine hepatocarcinogenesis</title><title>Carcinogenesis (New York)</title><addtitle>Carcinogenesis</addtitle><description>Curcumin has been widely used as a spice and coloring agent in foods. Recently, curcumin was found to possess chemopreventive effects against skin cancer, forestomach cancer, colon cancer and oral cancer in mice. Clinical trials of curcumin for prevention of human cancers are currently ongoing. In this study, we examine the chemopreventive effect of curcumin on murine hepatocarcinogenesis. C3H/HeN mice were injected i.p. with N-diethylnitrosamine (DEN) at the age of 5 weeks. The curcumin group started eating 0.2% curcumin-containing diet 4 days before DEN injection until death. The mice were then serially killed at the scheduled times to examine the development of hepatocellular carcinoma (HCC) and changes in intermediate biological markers. At the age of 42 weeks, the curcumin group, as compared with the control group (DEN alone), had an 81% reduction in multiplicity (0.5 versus 2.57) and a 62% reduction in incidence (38 versus 100%) of development of HCC. A series of intermediate biological markers were examined by western blot. While hepatic tissues obtained from the DEN-treated mice showed a remarkable increase in the levels of p21ras, PCNA and CDC2 proteins, eating a curcumin-containing diet reversed the levels to normal values. These results indicate that curcumin effectively inhibits DEN-induced hepatocarcinogenesis in the mouse. The underlying mechanisms of the phenomenon and the feasibility of using curcumin in the chemoprevention of human HCC should be further explored.</description><subject>12-O-tetradecanoylphorbol-13-acetate</subject><subject>Animals</subject><subject>Anti-Inflammatory Agents, Non-Steroidal - pharmacology</subject><subject>Anti-Inflammatory Agents, Non-Steroidal - therapeutic use</subject><subject>Anticarcinogenic Agents - therapeutic use</subject><subject>Biological and medical sciences</subject><subject>Biomarkers</subject><subject>Carcinogenesis, carcinogens and anticarcinogens</subject><subject>Carcinogens - toxicity</subject><subject>CDC2 Protein Kinase - analysis</subject><subject>Chemical and Drug Induced Liver Injury - prevention & control</subject><subject>curcumin</subject><subject>Curcumin - pharmacology</subject><subject>Curcumin - therapeutic use</subject><subject>DEN</subject><subject>Diet</subject><subject>Diethylnitrosamine - toxicity</subject><subject>Foods and miscellaneous</subject><subject>HCC</subject><subject>hepatocellular carcinoma</subject><subject>Liver - chemistry</subject><subject>Liver - drug effects</subject><subject>Liver Neoplasms, Experimental - chemically induced</subject><subject>Liver Neoplasms, Experimental - prevention & control</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred C3H</subject><subject>N-diethylnitrosamine</subject><subject>Neoplasm Proteins - analysis</subject><subject>PCNA</subject><subject>PKC</subject><subject>proliferating cell nuclear antigen</subject><subject>Proliferating Cell Nuclear Antigen - analysis</subject><subject>protein kinase C</subject><subject>Proto-Oncogene Proteins p21(ras) - analysis</subject><subject>Spices</subject><subject>TPA</subject><subject>Tumors</subject><issn>0143-3334</issn><issn>1460-2180</issn><issn>1460-2180</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0N1rFDEQAPAgir1Wn32TQ8S3vUsy-XyUo7VCQQWFoy8hm016qbvZM9kF-98bu4eKT8MwvxlmBqFXBG8I1rB1NruYtpRs6AaAPEErwgRuKFH4KVphwqABAHaGzku5x5gI4Po5OiNYcKmlWqH9bs5uHmJq3JgmG1NMd-su-mkd0yG2cSqP2eGhT3HKY7GV-iambna-Ww9zrun64I92GpddxjuffInlBXoWbF_8y1O8QN-uLr_urpubTx8-7t7fNI5TPjVSEaWsEiE41WrbKgaCsRYsWKK17FpNheCC1GAD-JZiFpyggdDgOYcOLtC7Ze4xjz9mXyYzxOJ839vkx7kYIjllQrMK3_wH78c5p7qboXW8kgrTirYLcvXWkn0wxxwHmx8Mweb3x81yZW0x1NSP147Xp7FzO_juH7-8uIK3J2CLs33INrlY_joAzaiorFlYLJP_-ads83cjJEhurve3Zv9Fks-3-yuj4BfJ65q4</recordid><startdate>20000201</startdate><enddate>20000201</enddate><creator>Chuang, S.E.</creator><creator>Kuo, M.L.</creator><creator>Hsu, C.H.</creator><creator>Chen, C.R.</creator><creator>Lin, J.K.</creator><creator>Lai, G.M.</creator><creator>Hsieh, C.Y.</creator><creator>Cheng, A.L.</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20000201</creationdate><title>Curcumin-containing diet inhibits diethylnitrosamine-induced murine hepatocarcinogenesis</title><author>Chuang, S.E. ; Kuo, M.L. ; Hsu, C.H. ; Chen, C.R. ; Lin, J.K. ; Lai, G.M. ; Hsieh, C.Y. ; Cheng, A.L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c525t-78188a86ffc8b9ab843644b3a3a1997db9266561926af3eb204fc62f12fe553d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>12-O-tetradecanoylphorbol-13-acetate</topic><topic>Animals</topic><topic>Anti-Inflammatory Agents, Non-Steroidal - pharmacology</topic><topic>Anti-Inflammatory Agents, Non-Steroidal - therapeutic use</topic><topic>Anticarcinogenic Agents - therapeutic use</topic><topic>Biological and medical sciences</topic><topic>Biomarkers</topic><topic>Carcinogenesis, carcinogens and anticarcinogens</topic><topic>Carcinogens - toxicity</topic><topic>CDC2 Protein Kinase - analysis</topic><topic>Chemical and Drug Induced Liver Injury - prevention & control</topic><topic>curcumin</topic><topic>Curcumin - pharmacology</topic><topic>Curcumin - therapeutic use</topic><topic>DEN</topic><topic>Diet</topic><topic>Diethylnitrosamine - toxicity</topic><topic>Foods and miscellaneous</topic><topic>HCC</topic><topic>hepatocellular carcinoma</topic><topic>Liver - chemistry</topic><topic>Liver - drug effects</topic><topic>Liver Neoplasms, Experimental - chemically induced</topic><topic>Liver Neoplasms, Experimental - prevention & control</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred C3H</topic><topic>N-diethylnitrosamine</topic><topic>Neoplasm Proteins - analysis</topic><topic>PCNA</topic><topic>PKC</topic><topic>proliferating cell nuclear antigen</topic><topic>Proliferating Cell Nuclear Antigen - analysis</topic><topic>protein kinase C</topic><topic>Proto-Oncogene Proteins p21(ras) - analysis</topic><topic>Spices</topic><topic>TPA</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chuang, S.E.</creatorcontrib><creatorcontrib>Kuo, M.L.</creatorcontrib><creatorcontrib>Hsu, C.H.</creatorcontrib><creatorcontrib>Chen, C.R.</creatorcontrib><creatorcontrib>Lin, J.K.</creatorcontrib><creatorcontrib>Lai, G.M.</creatorcontrib><creatorcontrib>Hsieh, C.Y.</creatorcontrib><creatorcontrib>Cheng, A.L.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Carcinogenesis (New York)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chuang, S.E.</au><au>Kuo, M.L.</au><au>Hsu, C.H.</au><au>Chen, C.R.</au><au>Lin, J.K.</au><au>Lai, G.M.</au><au>Hsieh, C.Y.</au><au>Cheng, A.L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Curcumin-containing diet inhibits diethylnitrosamine-induced murine hepatocarcinogenesis</atitle><jtitle>Carcinogenesis (New York)</jtitle><addtitle>Carcinogenesis</addtitle><date>2000-02-01</date><risdate>2000</risdate><volume>21</volume><issue>2</issue><spage>331</spage><epage>335</epage><pages>331-335</pages><issn>0143-3334</issn><issn>1460-2180</issn><eissn>1460-2180</eissn><coden>CRNGDP</coden><abstract>Curcumin has been widely used as a spice and coloring agent in foods. Recently, curcumin was found to possess chemopreventive effects against skin cancer, forestomach cancer, colon cancer and oral cancer in mice. Clinical trials of curcumin for prevention of human cancers are currently ongoing. In this study, we examine the chemopreventive effect of curcumin on murine hepatocarcinogenesis. C3H/HeN mice were injected i.p. with N-diethylnitrosamine (DEN) at the age of 5 weeks. The curcumin group started eating 0.2% curcumin-containing diet 4 days before DEN injection until death. The mice were then serially killed at the scheduled times to examine the development of hepatocellular carcinoma (HCC) and changes in intermediate biological markers. At the age of 42 weeks, the curcumin group, as compared with the control group (DEN alone), had an 81% reduction in multiplicity (0.5 versus 2.57) and a 62% reduction in incidence (38 versus 100%) of development of HCC. A series of intermediate biological markers were examined by western blot. While hepatic tissues obtained from the DEN-treated mice showed a remarkable increase in the levels of p21ras, PCNA and CDC2 proteins, eating a curcumin-containing diet reversed the levels to normal values. These results indicate that curcumin effectively inhibits DEN-induced hepatocarcinogenesis in the mouse. The underlying mechanisms of the phenomenon and the feasibility of using curcumin in the chemoprevention of human HCC should be further explored.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>10657978</pmid><doi>10.1093/carcin/21.2.331</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0143-3334 |
| ispartof | Carcinogenesis (New York), 2000-02, Vol.21 (2), p.331-335 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | 12-O-tetradecanoylphorbol-13-acetate Animals Anti-Inflammatory Agents, Non-Steroidal - pharmacology Anti-Inflammatory Agents, Non-Steroidal - therapeutic use Anticarcinogenic Agents - therapeutic use Biological and medical sciences Biomarkers Carcinogenesis, carcinogens and anticarcinogens Carcinogens - toxicity CDC2 Protein Kinase - analysis Chemical and Drug Induced Liver Injury - prevention & control curcumin Curcumin - pharmacology Curcumin - therapeutic use DEN Diet Diethylnitrosamine - toxicity Foods and miscellaneous HCC hepatocellular carcinoma Liver - chemistry Liver - drug effects Liver Neoplasms, Experimental - chemically induced Liver Neoplasms, Experimental - prevention & control Male Medical sciences Mice Mice, Inbred C3H N-diethylnitrosamine Neoplasm Proteins - analysis PCNA PKC proliferating cell nuclear antigen Proliferating Cell Nuclear Antigen - analysis protein kinase C Proto-Oncogene Proteins p21(ras) - analysis Spices TPA Tumors |
| title | Curcumin-containing diet inhibits diethylnitrosamine-induced murine hepatocarcinogenesis |
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