Genome-Wide Microarray Analysis of Long Non-Coding RNAs in Eutopic Secretory Endometrium with Endometriosis
Background/Aims: Dysregulated long non-coding RNAs (lncRNAs) can lead to the occurrence of various diseases; however, reports of the function of lncRNAs in endometriosis and related studies are scarce. The pathogenesis of endometriosis is still poorly understood. Methods: Dysregulated lncRNAs and mR...
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| Veröffentlicht in: | Cellular physiology and biochemistry 2015-01, Vol.37 (6), p.2231-2245 |
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| creator | Wang, Yang Li, Yan Yang, Zhuo Liu, Kuiran Wang, Danbo |
| description | Background/Aims: Dysregulated long non-coding RNAs (lncRNAs) can lead to the occurrence of various diseases; however, reports of the function of lncRNAs in endometriosis and related studies are scarce. The pathogenesis of endometriosis is still poorly understood. Methods: Dysregulated lncRNAs and mRNAs between eutopic and normal endometrium (both are late secretory) were analyzed by lncRNA microarray. Eight lncRNAs and mRNA CDK6 were validated using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Bioinformatics prediction was used to investigate the potential function of these differentially expressed lncRNAs. Results: Microarray expression profiling suggests 1277 lncRNAs (488 up- and 789 down-regulated) and 1216 mRNAs (578 up- and 638 down-regulated) were expressed differentially between eutopic and normal endometrium. Pathway analysis and gene ontology (GO) analysis found differently expressed lncRNAs associated with the cell cycle and immune regulation. The relative level of expression of CDK6 and AC002454.1 were obtained by qRT-PCR and the Pearson correlation coefficient was 0.747 (p |
| doi_str_mv | 10.1159/000438579 |
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The pathogenesis of endometriosis is still poorly understood. Methods: Dysregulated lncRNAs and mRNAs between eutopic and normal endometrium (both are late secretory) were analyzed by lncRNA microarray. Eight lncRNAs and mRNA CDK6 were validated using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Bioinformatics prediction was used to investigate the potential function of these differentially expressed lncRNAs. Results: Microarray expression profiling suggests 1277 lncRNAs (488 up- and 789 down-regulated) and 1216 mRNAs (578 up- and 638 down-regulated) were expressed differentially between eutopic and normal endometrium. Pathway analysis and gene ontology (GO) analysis found differently expressed lncRNAs associated with the cell cycle and immune regulation. The relative level of expression of CDK6 and AC002454.1 were obtained by qRT-PCR and the Pearson correlation coefficient was 0.747 (p<0.0001). A coding-noncoding gene co-expression (CNC) network was constructed for these validated lncRNAs. Conclusion: These dysregulated lncRNAs might provide information for new biomarkers or novel therapeutic targets of endometriosis. AC002454.1 might induce cell cycle disorder by regulating CDK6 to participate in the pathogenesis of endometriosis.</description><identifier>ISSN: 1015-8987</identifier><identifier>EISSN: 1421-9778</identifier><identifier>DOI: 10.1159/000438579</identifier><identifier>PMID: 26618670</identifier><language>eng</language><publisher>Basel, Switzerland: Cell Physiol Biochem Press GmbH & Co KG</publisher><subject>Adult ; CDK6 ; Cell cycle ; Endometriosis ; Endometriosis - genetics ; Endometrium - metabolism ; Endometrium - pathology ; Eutopic endometrium ; Female ; Genome-Wide Association Study ; Humans ; LncRNA ; Microarray ; Middle Aged ; Oligonucleotide Array Sequence Analysis ; Original Paper ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Long Noncoding - genetics</subject><ispartof>Cellular physiology and biochemistry, 2015-01, Vol.37 (6), p.2231-2245</ispartof><rights>2015 The Author(s) Published by S. Karger AG, Basel</rights><rights>2015 The Author(s) Published by S. Karger AG, Basel.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c407t-66f77019d5189a7791e389d89f700623386e576b011db3e40077fb797ef078b23</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,2102,27635,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26618670$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Yang</creatorcontrib><creatorcontrib>Li, Yan</creatorcontrib><creatorcontrib>Yang, Zhuo</creatorcontrib><creatorcontrib>Liu, Kuiran</creatorcontrib><creatorcontrib>Wang, Danbo</creatorcontrib><title>Genome-Wide Microarray Analysis of Long Non-Coding RNAs in Eutopic Secretory Endometrium with Endometriosis</title><title>Cellular physiology and biochemistry</title><addtitle>Cell Physiol Biochem</addtitle><description>Background/Aims: Dysregulated long non-coding RNAs (lncRNAs) can lead to the occurrence of various diseases; however, reports of the function of lncRNAs in endometriosis and related studies are scarce. The pathogenesis of endometriosis is still poorly understood. Methods: Dysregulated lncRNAs and mRNAs between eutopic and normal endometrium (both are late secretory) were analyzed by lncRNA microarray. Eight lncRNAs and mRNA CDK6 were validated using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Bioinformatics prediction was used to investigate the potential function of these differentially expressed lncRNAs. Results: Microarray expression profiling suggests 1277 lncRNAs (488 up- and 789 down-regulated) and 1216 mRNAs (578 up- and 638 down-regulated) were expressed differentially between eutopic and normal endometrium. Pathway analysis and gene ontology (GO) analysis found differently expressed lncRNAs associated with the cell cycle and immune regulation. The relative level of expression of CDK6 and AC002454.1 were obtained by qRT-PCR and the Pearson correlation coefficient was 0.747 (p<0.0001). A coding-noncoding gene co-expression (CNC) network was constructed for these validated lncRNAs. Conclusion: These dysregulated lncRNAs might provide information for new biomarkers or novel therapeutic targets of endometriosis. AC002454.1 might induce cell cycle disorder by regulating CDK6 to participate in the pathogenesis of endometriosis.</description><subject>Adult</subject><subject>CDK6</subject><subject>Cell cycle</subject><subject>Endometriosis</subject><subject>Endometriosis - genetics</subject><subject>Endometrium - metabolism</subject><subject>Endometrium - pathology</subject><subject>Eutopic endometrium</subject><subject>Female</subject><subject>Genome-Wide Association Study</subject><subject>Humans</subject><subject>LncRNA</subject><subject>Microarray</subject><subject>Middle Aged</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Original Paper</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Long Noncoding - genetics</subject><issn>1015-8987</issn><issn>1421-9778</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>M--</sourceid><sourceid>EIF</sourceid><sourceid>DOA</sourceid><recordid>eNpFkU2P0zAQhiMEYpeFA3eEfIRDwE5sj32sqrKsVBaJD3G0nHhcvJvEXTsR6r_H0FJOHo-eeUaat6peMvqOMaHfU0p5qwToR9Ul4w2rNYB6XGrKRK20govqWc53tHxBN0-ri0ZKpiTQy-r-Gqc4Yv0jOCSfQp-iTckeyGqywyGHTKIn2zjtyG2c6nV0oZRfbleZhIlsljnuQ0--Yp9wjulANpMrsjmFZSS_wvzzfyMW1_PqibdDxhen96r6_mHzbf2x3n6-vlmvtnXPKcy1lB6AMu0EU9oCaIat0k5pD5TKpm2VRAGyo4y5rkVOKYDvQAN6Cqpr2qvq5uh10d6ZfQqjTQcTbTB_GzHtjE1z6Ac0nkuuyska6S3vJNeO9Rw60aIGbQUW15uja5_iw4J5NmPIPQ6DnTAu2TAQTSsYcF7Qt0e0HDHnhP68mlHzJydzzqmwr0_apRvRncl_wRTg1RG4t2mH6Qyc5n8DUKSUQw</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Wang, Yang</creator><creator>Li, Yan</creator><creator>Yang, Zhuo</creator><creator>Liu, Kuiran</creator><creator>Wang, Danbo</creator><general>Cell Physiol Biochem Press GmbH & Co KG</general><scope>M--</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>DOA</scope></search><sort><creationdate>20150101</creationdate><title>Genome-Wide Microarray Analysis of Long Non-Coding RNAs in Eutopic Secretory Endometrium with Endometriosis</title><author>Wang, Yang ; Li, Yan ; Yang, Zhuo ; Liu, Kuiran ; Wang, Danbo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c407t-66f77019d5189a7791e389d89f700623386e576b011db3e40077fb797ef078b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adult</topic><topic>CDK6</topic><topic>Cell cycle</topic><topic>Endometriosis</topic><topic>Endometriosis - genetics</topic><topic>Endometrium - metabolism</topic><topic>Endometrium - pathology</topic><topic>Eutopic endometrium</topic><topic>Female</topic><topic>Genome-Wide Association Study</topic><topic>Humans</topic><topic>LncRNA</topic><topic>Microarray</topic><topic>Middle Aged</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Original Paper</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Long Noncoding - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Yang</creatorcontrib><creatorcontrib>Li, Yan</creatorcontrib><creatorcontrib>Yang, Zhuo</creatorcontrib><creatorcontrib>Liu, Kuiran</creatorcontrib><creatorcontrib>Wang, Danbo</creatorcontrib><collection>Karger Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Cellular physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Yang</au><au>Li, Yan</au><au>Yang, Zhuo</au><au>Liu, Kuiran</au><au>Wang, Danbo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genome-Wide Microarray Analysis of Long Non-Coding RNAs in Eutopic Secretory Endometrium with Endometriosis</atitle><jtitle>Cellular physiology and biochemistry</jtitle><addtitle>Cell Physiol Biochem</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>37</volume><issue>6</issue><spage>2231</spage><epage>2245</epage><pages>2231-2245</pages><issn>1015-8987</issn><eissn>1421-9778</eissn><abstract>Background/Aims: Dysregulated long non-coding RNAs (lncRNAs) can lead to the occurrence of various diseases; however, reports of the function of lncRNAs in endometriosis and related studies are scarce. The pathogenesis of endometriosis is still poorly understood. Methods: Dysregulated lncRNAs and mRNAs between eutopic and normal endometrium (both are late secretory) were analyzed by lncRNA microarray. Eight lncRNAs and mRNA CDK6 were validated using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Bioinformatics prediction was used to investigate the potential function of these differentially expressed lncRNAs. Results: Microarray expression profiling suggests 1277 lncRNAs (488 up- and 789 down-regulated) and 1216 mRNAs (578 up- and 638 down-regulated) were expressed differentially between eutopic and normal endometrium. Pathway analysis and gene ontology (GO) analysis found differently expressed lncRNAs associated with the cell cycle and immune regulation. The relative level of expression of CDK6 and AC002454.1 were obtained by qRT-PCR and the Pearson correlation coefficient was 0.747 (p<0.0001). A coding-noncoding gene co-expression (CNC) network was constructed for these validated lncRNAs. Conclusion: These dysregulated lncRNAs might provide information for new biomarkers or novel therapeutic targets of endometriosis. AC002454.1 might induce cell cycle disorder by regulating CDK6 to participate in the pathogenesis of endometriosis.</abstract><cop>Basel, Switzerland</cop><pub>Cell Physiol Biochem Press GmbH & Co KG</pub><pmid>26618670</pmid><doi>10.1159/000438579</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult CDK6 Cell cycle Endometriosis Endometriosis - genetics Endometrium - metabolism Endometrium - pathology Eutopic endometrium Female Genome-Wide Association Study Humans LncRNA Microarray Middle Aged Oligonucleotide Array Sequence Analysis Original Paper Reverse Transcriptase Polymerase Chain Reaction RNA, Long Noncoding - genetics |
| title | Genome-Wide Microarray Analysis of Long Non-Coding RNAs in Eutopic Secretory Endometrium with Endometriosis |
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