Cruciferous phytoalexins: antiproliferative effects in T-Jurkat leukemic cells
We tested antiproliferative activity of selected cruciferous phytoalexins including brassinin, 1-methoxybrassinin, (±)-spirobrassin, (±)-1-methoxyspirobrassinin and (±)-1-methoxyspirobrassinol, in leukemic Jurkat cell. The most effective of the tested phytoalexins was 1-methoxybrassinin with IC 50 1...
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| Veröffentlicht in: | Leukemia research 2005-04, Vol.29 (4), p.415-421 |
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| creator | Pilátová, M. Šarišský, M. Kutschy, P. Miroššay, A. Mezencev, R. Čurillová, Z. Suchý, M. Monde, K. Mirossay, L. Mojžiš, J. |
| description | We tested antiproliferative activity of selected cruciferous phytoalexins including brassinin, 1-methoxybrassinin, (±)-spirobrassin, (±)-1-methoxyspirobrassinin and (±)-1-methoxyspirobrassinol, in leukemic Jurkat cell. The most effective of the tested phytoalexins was 1-methoxybrassinin with IC
50 10
μmol
l
−1. However, significant effect of all phytoalexines was also determined at concentration 1
μmol
l
−1. In 1-methoxybrassinin-treated Jurkat cells, we found significant increase in the fraction of cells with a sub-G
0/G
1 DNA content, which is considered to be a marker of cell death by apoptosis. Apoptosis was also confirmed by the annexin V staining.
In summary, 1-methoxybrassinin exerted potent antiproliferative activity probably due to cell cycle arrest and apoptosis induction. |
| doi_str_mv | 10.1016/j.leukres.2004.09.003 |
| format | Article |
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50 10
μmol
l
−1. However, significant effect of all phytoalexines was also determined at concentration 1
μmol
l
−1. In 1-methoxybrassinin-treated Jurkat cells, we found significant increase in the fraction of cells with a sub-G
0/G
1 DNA content, which is considered to be a marker of cell death by apoptosis. Apoptosis was also confirmed by the annexin V staining.
In summary, 1-methoxybrassinin exerted potent antiproliferative activity probably due to cell cycle arrest and apoptosis induction.</description><identifier>ISSN: 0145-2126</identifier><identifier>EISSN: 1873-5835</identifier><identifier>DOI: 10.1016/j.leukres.2004.09.003</identifier><identifier>PMID: 15725476</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Antiproliferative effects ; Apoptosis ; Apoptosis - drug effects ; Brassicaceae ; Cell Death - drug effects ; Cruciferous phytoalexins ; Dose-Response Relationship, Drug ; Humans ; Jurkat Cells - drug effects ; Leukemia ; Phytotherapy ; Plant Extracts - toxicity ; Sesquiterpenes ; Terpenes</subject><ispartof>Leukemia research, 2005-04, Vol.29 (4), p.415-421</ispartof><rights>2004 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c394t-514d13d198c3a7d951b46761e427a1663d980419e9b616adfc27ae4dab6383cf3</citedby><cites>FETCH-LOGICAL-c394t-514d13d198c3a7d951b46761e427a1663d980419e9b616adfc27ae4dab6383cf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.leukres.2004.09.003$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15725476$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pilátová, M.</creatorcontrib><creatorcontrib>Šarišský, M.</creatorcontrib><creatorcontrib>Kutschy, P.</creatorcontrib><creatorcontrib>Miroššay, A.</creatorcontrib><creatorcontrib>Mezencev, R.</creatorcontrib><creatorcontrib>Čurillová, Z.</creatorcontrib><creatorcontrib>Suchý, M.</creatorcontrib><creatorcontrib>Monde, K.</creatorcontrib><creatorcontrib>Mirossay, L.</creatorcontrib><creatorcontrib>Mojžiš, J.</creatorcontrib><title>Cruciferous phytoalexins: antiproliferative effects in T-Jurkat leukemic cells</title><title>Leukemia research</title><addtitle>Leuk Res</addtitle><description>We tested antiproliferative activity of selected cruciferous phytoalexins including brassinin, 1-methoxybrassinin, (±)-spirobrassin, (±)-1-methoxyspirobrassinin and (±)-1-methoxyspirobrassinol, in leukemic Jurkat cell. The most effective of the tested phytoalexins was 1-methoxybrassinin with IC
50 10
μmol
l
−1. However, significant effect of all phytoalexines was also determined at concentration 1
μmol
l
−1. In 1-methoxybrassinin-treated Jurkat cells, we found significant increase in the fraction of cells with a sub-G
0/G
1 DNA content, which is considered to be a marker of cell death by apoptosis. Apoptosis was also confirmed by the annexin V staining.
In summary, 1-methoxybrassinin exerted potent antiproliferative activity probably due to cell cycle arrest and apoptosis induction.</description><subject>Antiproliferative effects</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Brassicaceae</subject><subject>Cell Death - drug effects</subject><subject>Cruciferous phytoalexins</subject><subject>Dose-Response Relationship, Drug</subject><subject>Humans</subject><subject>Jurkat Cells - drug effects</subject><subject>Leukemia</subject><subject>Phytotherapy</subject><subject>Plant Extracts - toxicity</subject><subject>Sesquiterpenes</subject><subject>Terpenes</subject><issn>0145-2126</issn><issn>1873-5835</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE9v2zAMxYWhw5ql_QgbfOrNnmj9sbVLUQTt2iHYLtlZkCUaU-LYmWQHzbefjATosScC5CP53o-QL0ALoCC_bYsOp13AWJSU8oKqglL2gSygrlguaiauyIICF3kJpbwmn2PcUkqFAvWJXIOoSsEruSC_VmGyvsUwTDE7_D2Ng-nw1ffxe2b60R_C0M1TM_ojZti2aMeY-T7b5D-nsDNjNrvAvbeZxa6LN-Rja7qIt5e6JH-eHjer53z9-8fL6mGdW6b4mAvgDpgDVVtmKqcENFxWEpCXlQEpmVM15aBQNRKkca1NfeTONJLVzLZsSe7Od5PBfxPGUe99nB2YHlMSDZVIWBKKJRFnoQ1DjAFbfQh-b8JJA9UzSL3VF5B6Bqmp0glk2vt6eTA1e3RvWxdySXB_FmCKefQYdLQee4vOhwRJu8G_8-I_zQ-H7Q</recordid><startdate>20050401</startdate><enddate>20050401</enddate><creator>Pilátová, M.</creator><creator>Šarišský, M.</creator><creator>Kutschy, P.</creator><creator>Miroššay, A.</creator><creator>Mezencev, R.</creator><creator>Čurillová, Z.</creator><creator>Suchý, M.</creator><creator>Monde, K.</creator><creator>Mirossay, L.</creator><creator>Mojžiš, J.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>20050401</creationdate><title>Cruciferous phytoalexins: antiproliferative effects in T-Jurkat leukemic cells</title><author>Pilátová, M. ; Šarišský, M. ; Kutschy, P. ; Miroššay, A. ; Mezencev, R. ; Čurillová, Z. ; Suchý, M. ; Monde, K. ; Mirossay, L. ; Mojžiš, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c394t-514d13d198c3a7d951b46761e427a1663d980419e9b616adfc27ae4dab6383cf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Antiproliferative effects</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Brassicaceae</topic><topic>Cell Death - drug effects</topic><topic>Cruciferous phytoalexins</topic><topic>Dose-Response Relationship, Drug</topic><topic>Humans</topic><topic>Jurkat Cells - drug effects</topic><topic>Leukemia</topic><topic>Phytotherapy</topic><topic>Plant Extracts - toxicity</topic><topic>Sesquiterpenes</topic><topic>Terpenes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pilátová, M.</creatorcontrib><creatorcontrib>Šarišský, M.</creatorcontrib><creatorcontrib>Kutschy, P.</creatorcontrib><creatorcontrib>Miroššay, A.</creatorcontrib><creatorcontrib>Mezencev, R.</creatorcontrib><creatorcontrib>Čurillová, Z.</creatorcontrib><creatorcontrib>Suchý, M.</creatorcontrib><creatorcontrib>Monde, K.</creatorcontrib><creatorcontrib>Mirossay, L.</creatorcontrib><creatorcontrib>Mojžiš, J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Leukemia research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pilátová, M.</au><au>Šarišský, M.</au><au>Kutschy, P.</au><au>Miroššay, A.</au><au>Mezencev, R.</au><au>Čurillová, Z.</au><au>Suchý, M.</au><au>Monde, K.</au><au>Mirossay, L.</au><au>Mojžiš, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cruciferous phytoalexins: antiproliferative effects in T-Jurkat leukemic cells</atitle><jtitle>Leukemia research</jtitle><addtitle>Leuk Res</addtitle><date>2005-04-01</date><risdate>2005</risdate><volume>29</volume><issue>4</issue><spage>415</spage><epage>421</epage><pages>415-421</pages><issn>0145-2126</issn><eissn>1873-5835</eissn><abstract>We tested antiproliferative activity of selected cruciferous phytoalexins including brassinin, 1-methoxybrassinin, (±)-spirobrassin, (±)-1-methoxyspirobrassinin and (±)-1-methoxyspirobrassinol, in leukemic Jurkat cell. The most effective of the tested phytoalexins was 1-methoxybrassinin with IC
50 10
μmol
l
−1. However, significant effect of all phytoalexines was also determined at concentration 1
μmol
l
−1. In 1-methoxybrassinin-treated Jurkat cells, we found significant increase in the fraction of cells with a sub-G
0/G
1 DNA content, which is considered to be a marker of cell death by apoptosis. Apoptosis was also confirmed by the annexin V staining.
In summary, 1-methoxybrassinin exerted potent antiproliferative activity probably due to cell cycle arrest and apoptosis induction.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>15725476</pmid><doi>10.1016/j.leukres.2004.09.003</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| subjects | Antiproliferative effects Apoptosis Apoptosis - drug effects Brassicaceae Cell Death - drug effects Cruciferous phytoalexins Dose-Response Relationship, Drug Humans Jurkat Cells - drug effects Leukemia Phytotherapy Plant Extracts - toxicity Sesquiterpenes Terpenes |
| title | Cruciferous phytoalexins: antiproliferative effects in T-Jurkat leukemic cells |
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