Short communication: Identification of iron-binding peptides from whey protein hydrolysates using iron (III)-immobilized metal ion affinity chromatography and reversed phase-HPLC-tandem mass spectrometry

Peptides with iron-binding capacity obtained by hydrolysis of whey protein with Alcalase (Novozymes, Araucaria, PR, Brazil), pancreatin, and Flavourzyme (Novozymes) were identified. Hydrolysates were subjected to iron (III)-immobilized metal ion affinity chromatography, and the bound peptides were s...

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Veröffentlicht in:	Journal of dairy science 2016-01, Vol.99 (1), p.77-82
Hauptverfasser:	Cruz-Huerta, Elvia, Martínez Maqueda, Daniel, de la Hoz, Lucia, da Silva, Vera S Nunes, Pacheco, Maria Teresa Bertoldo, Amigo, Lourdes, Recio, Isidra
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acids - analysis Chromatography, Affinity Chromatography, High Pressure Liquid Chromatography, Reverse-Phase Iron-Binding Proteins - analysis Protein Hydrolysates - analysis Tandem Mass Spectrometry Whey Proteins - analysis
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