Introduction of the exogenous NADH coenzyme regeneration system and its influence on intracellular metabolic flux of Paenibacillus polymyxa

•pMA5 plasmid and P43 promoter provide a new tool for expression of exogenous genes in P. polymyxa.•The NAD+-dependent formate dehydrogenase was successfully expressed in P. polymyxa.•There is no need to supplement formic acid during fermentation.•The yield of R,R-2,3-butanediol was improved through...

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Veröffentlicht in:	Bioresource technology 2016-02, Vol.201, p.319-328
Hauptverfasser:	Zhang, Li, Xu, Youyong, Gao, Jian, Xu, Hong, Cao, Can, Xue, Feng, Ding, Ge, Peng, Yingyun
Format:	Artikel
Sprache:	eng
Schlagworte:	Batch Cell Culture Techniques Biosynthetic Pathways Butylene Glycols - metabolism Coenzymes - metabolism Fermentation Formate dehydrogenase Formate Dehydrogenases - genetics Formate Dehydrogenases - metabolism Genes, Bacterial Genetic Vectors - metabolism Intracellular Space - metabolism Metabolic flux Metabolic Flux Analysis NAD - metabolism NADH regeneration Paenibacillus - enzymology Paenibacillus - genetics Paenibacillus - growth & development Paenibacillus - metabolism Paenibacillus polymyxa R,R-2,3-butanediol
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