Serum albumin coating of demineralized bone matrix results in stronger new bone formation
Blood serum fractions are hotly debated adjuvants in bone replacement therapies. In the present experiment, we coated demineralized bone matrices (DBM) with serum albumin and investigated stem cell attachment in vitro and bone formation in a rat calvaria defect model. In the in vitro experiments, we...
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| Veröffentlicht in: | Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2016-01, Vol.104 (1), p.126-132 |
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| creator | Horváthy, Dénes B. Vácz, Gabriella Szabó, Tamás Szigyártó, Imola C. Toró, Ildikó Vámos, Boglárka Hornyák, István Renner, Károly Klára, Tamás Szabó, Bence T. Dobó-Nagy, Csaba Doros, Attila Lacza, Zsombor |
| description | Blood serum fractions are hotly debated adjuvants in bone replacement therapies. In the present experiment, we coated demineralized bone matrices (DBM) with serum albumin and investigated stem cell attachment in vitro and bone formation in a rat calvaria defect model. In the in vitro experiments, we observed that significantly more cells adhere to the serum albumin coated DBMs at every time point. In vivo bone formation with albumin coated and uncoated DBM was monitored biweekly by computed tomography until 11 weeks postoperatively while empty defects served as controls. By the seventh week, the bone defect in the albumin group was almost completely closed (remaining defect 3.0 ± 2.3%), while uncoated DBM and unfilled control groups still had significant defects (uncoated: 40.2 ± 9.1%, control: 52.4 ± 8.9%). Higher density values were also observed in the albumin coated DBM group. In addition, the serum albumin enhanced group showed significantly higher volume of newly formed bone in the microCT analysis and produced significantly higher breaking force and stiffness compared to the uncoated grafts (peak breaking force: uncoated: 15.7 ± 4 N, albumin 46.1 ± 11 N). In conclusion, this investigation shows that implanting serum albumin coated DBM significantly reduces healing period in nonhealing defects and results in mechanically stronger bone. These results also support the idea that serum albumin coating provides a convenient milieu for stem cell function, and a much improved bone grafting success can be achieved without the use of exogenous stem cells. |
| doi_str_mv | 10.1002/jbm.b.33359 |
| format | Article |
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In the present experiment, we coated demineralized bone matrices (DBM) with serum albumin and investigated stem cell attachment in vitro and bone formation in a rat calvaria defect model. In the in vitro experiments, we observed that significantly more cells adhere to the serum albumin coated DBMs at every time point. In vivo bone formation with albumin coated and uncoated DBM was monitored biweekly by computed tomography until 11 weeks postoperatively while empty defects served as controls. By the seventh week, the bone defect in the albumin group was almost completely closed (remaining defect 3.0 ± 2.3%), while uncoated DBM and unfilled control groups still had significant defects (uncoated: 40.2 ± 9.1%, control: 52.4 ± 8.9%). Higher density values were also observed in the albumin coated DBM group. In addition, the serum albumin enhanced group showed significantly higher volume of newly formed bone in the microCT analysis and produced significantly higher breaking force and stiffness compared to the uncoated grafts (peak breaking force: uncoated: 15.7 ± 4 N, albumin 46.1 ± 11 N). In conclusion, this investigation shows that implanting serum albumin coated DBM significantly reduces healing period in nonhealing defects and results in mechanically stronger bone. These results also support the idea that serum albumin coating provides a convenient milieu for stem cell function, and a much improved bone grafting success can be achieved without the use of exogenous stem cells.</description><identifier>ISSN: 1552-4973</identifier><identifier>EISSN: 1552-4981</identifier><identifier>DOI: 10.1002/jbm.b.33359</identifier><identifier>PMID: 25677203</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Adjuvants ; Albumin ; Animals ; Biomedical materials ; bone ; Bone Demineralization Technique ; Bone grafts ; Bone growth ; Bone healing ; Bone matrix ; Breaking ; Calvaria ; calvaria defect ; Cell Adhesion ; Coated Materials, Biocompatible - chemistry ; Coated Materials, Biocompatible - pharmacology ; Coating ; Computed tomography ; DBM ; Defects ; Demineralizing ; Extracellular Matrix - chemistry ; Grafts ; Healing ; In vitro methods and tests ; Male ; Materials research ; Materials science ; Osteogenesis ; Osteogenesis - drug effects ; Rats ; Rats, Wistar ; Serum Albumin ; Skull - injuries ; Skull - metabolism ; Skull - pathology ; Stem cell transplantation ; Stem cells ; Stem Cells - metabolism ; Stiffness ; Surgical implants</subject><ispartof>Journal of biomedical materials research. 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Part B, Applied biomaterials</title><addtitle>J. Biomed. Mater. Res</addtitle><description>Blood serum fractions are hotly debated adjuvants in bone replacement therapies. In the present experiment, we coated demineralized bone matrices (DBM) with serum albumin and investigated stem cell attachment in vitro and bone formation in a rat calvaria defect model. In the in vitro experiments, we observed that significantly more cells adhere to the serum albumin coated DBMs at every time point. In vivo bone formation with albumin coated and uncoated DBM was monitored biweekly by computed tomography until 11 weeks postoperatively while empty defects served as controls. By the seventh week, the bone defect in the albumin group was almost completely closed (remaining defect 3.0 ± 2.3%), while uncoated DBM and unfilled control groups still had significant defects (uncoated: 40.2 ± 9.1%, control: 52.4 ± 8.9%). Higher density values were also observed in the albumin coated DBM group. In addition, the serum albumin enhanced group showed significantly higher volume of newly formed bone in the microCT analysis and produced significantly higher breaking force and stiffness compared to the uncoated grafts (peak breaking force: uncoated: 15.7 ± 4 N, albumin 46.1 ± 11 N). In conclusion, this investigation shows that implanting serum albumin coated DBM significantly reduces healing period in nonhealing defects and results in mechanically stronger bone. These results also support the idea that serum albumin coating provides a convenient milieu for stem cell function, and a much improved bone grafting success can be achieved without the use of exogenous stem cells.</description><subject>Adjuvants</subject><subject>Albumin</subject><subject>Animals</subject><subject>Biomedical materials</subject><subject>bone</subject><subject>Bone Demineralization Technique</subject><subject>Bone grafts</subject><subject>Bone growth</subject><subject>Bone healing</subject><subject>Bone matrix</subject><subject>Breaking</subject><subject>Calvaria</subject><subject>calvaria defect</subject><subject>Cell Adhesion</subject><subject>Coated Materials, Biocompatible - chemistry</subject><subject>Coated Materials, Biocompatible - pharmacology</subject><subject>Coating</subject><subject>Computed tomography</subject><subject>DBM</subject><subject>Defects</subject><subject>Demineralizing</subject><subject>Extracellular Matrix - chemistry</subject><subject>Grafts</subject><subject>Healing</subject><subject>In vitro methods and tests</subject><subject>Male</subject><subject>Materials research</subject><subject>Materials science</subject><subject>Osteogenesis</subject><subject>Osteogenesis - drug effects</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Serum Albumin</subject><subject>Skull - injuries</subject><subject>Skull - metabolism</subject><subject>Skull - pathology</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Stem Cells - metabolism</subject><subject>Stiffness</subject><subject>Surgical implants</subject><issn>1552-4973</issn><issn>1552-4981</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90UlLxDAYBuAgisvoybsEvAjSMcukmRzFZRREwRVPIUm_Sse2GZMWl19vdHQOHjwlJE9ePvIitE3JkBLCDqa2Gdoh51yoJbROhWDZSI3p8mIv-RraiHGacE4EX0VrTORSMsLX0eMNhL7BprZ9U7XYedNV7RP2JS4gHUAwdfUBBba-BdyYLlRvOEDs6y7i5GMXfPsEAbfwOjelD4lVvt1EK6WpI2z9rAN0d3pye3SWXVxNzo8OLzInqOoyqRQnghbKKVGOVcFkLhUDImWuSOmYLRRzDiQ1wljDlRo5w42A9GRMqCV8gPbmubPgX3qInW6q6KCuTQu-j5pKQXNJv75hgHb_0KnvQ5um01TRkZRiRMi_KmURxVieJ7U_Vy74GAOUehaqxoR3TYn-6kWnXrTV370kvfOT2dsGioX9LSKBbA6q2MHb4t6EZ51LLoV-uJzoe3Ys1Q2Z6Gv-CYLUlrc</recordid><startdate>20160101</startdate><enddate>20160101</enddate><creator>Horváthy, Dénes B.</creator><creator>Vácz, Gabriella</creator><creator>Szabó, Tamás</creator><creator>Szigyártó, Imola C.</creator><creator>Toró, Ildikó</creator><creator>Vámos, Boglárka</creator><creator>Hornyák, István</creator><creator>Renner, Károly</creator><creator>Klára, Tamás</creator><creator>Szabó, Bence T.</creator><creator>Dobó-Nagy, Csaba</creator><creator>Doros, Attila</creator><creator>Lacza, Zsombor</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20160101</creationdate><title>Serum albumin coating of demineralized bone matrix results in stronger new bone formation</title><author>Horváthy, Dénes B. ; Vácz, Gabriella ; Szabó, Tamás ; Szigyártó, Imola C. ; Toró, Ildikó ; Vámos, Boglárka ; Hornyák, István ; Renner, Károly ; Klára, Tamás ; Szabó, Bence T. ; Dobó-Nagy, Csaba ; Doros, Attila ; Lacza, Zsombor</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c519t-7993051d9c95f89d276792e077690fc2bd92cce71a5aba3994ca3a5e51d801b03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adjuvants</topic><topic>Albumin</topic><topic>Animals</topic><topic>Biomedical materials</topic><topic>bone</topic><topic>Bone Demineralization Technique</topic><topic>Bone grafts</topic><topic>Bone growth</topic><topic>Bone healing</topic><topic>Bone matrix</topic><topic>Breaking</topic><topic>Calvaria</topic><topic>calvaria defect</topic><topic>Cell Adhesion</topic><topic>Coated Materials, Biocompatible - chemistry</topic><topic>Coated Materials, Biocompatible - pharmacology</topic><topic>Coating</topic><topic>Computed tomography</topic><topic>DBM</topic><topic>Defects</topic><topic>Demineralizing</topic><topic>Extracellular Matrix - chemistry</topic><topic>Grafts</topic><topic>Healing</topic><topic>In vitro methods and tests</topic><topic>Male</topic><topic>Materials research</topic><topic>Materials science</topic><topic>Osteogenesis</topic><topic>Osteogenesis - drug effects</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Serum Albumin</topic><topic>Skull - injuries</topic><topic>Skull - metabolism</topic><topic>Skull - pathology</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>Stem Cells - metabolism</topic><topic>Stiffness</topic><topic>Surgical implants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Horváthy, Dénes B.</creatorcontrib><creatorcontrib>Vácz, Gabriella</creatorcontrib><creatorcontrib>Szabó, Tamás</creatorcontrib><creatorcontrib>Szigyártó, Imola C.</creatorcontrib><creatorcontrib>Toró, Ildikó</creatorcontrib><creatorcontrib>Vámos, Boglárka</creatorcontrib><creatorcontrib>Hornyák, István</creatorcontrib><creatorcontrib>Renner, Károly</creatorcontrib><creatorcontrib>Klára, Tamás</creatorcontrib><creatorcontrib>Szabó, Bence T.</creatorcontrib><creatorcontrib>Dobó-Nagy, Csaba</creatorcontrib><creatorcontrib>Doros, Attila</creatorcontrib><creatorcontrib>Lacza, Zsombor</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biomedical materials research. Part B, Applied biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Horváthy, Dénes B.</au><au>Vácz, Gabriella</au><au>Szabó, Tamás</au><au>Szigyártó, Imola C.</au><au>Toró, Ildikó</au><au>Vámos, Boglárka</au><au>Hornyák, István</au><au>Renner, Károly</au><au>Klára, Tamás</au><au>Szabó, Bence T.</au><au>Dobó-Nagy, Csaba</au><au>Doros, Attila</au><au>Lacza, Zsombor</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serum albumin coating of demineralized bone matrix results in stronger new bone formation</atitle><jtitle>Journal of biomedical materials research. Part B, Applied biomaterials</jtitle><addtitle>J. Biomed. Mater. Res</addtitle><date>2016-01-01</date><risdate>2016</risdate><volume>104</volume><issue>1</issue><spage>126</spage><epage>132</epage><pages>126-132</pages><issn>1552-4973</issn><eissn>1552-4981</eissn><abstract>Blood serum fractions are hotly debated adjuvants in bone replacement therapies. In the present experiment, we coated demineralized bone matrices (DBM) with serum albumin and investigated stem cell attachment in vitro and bone formation in a rat calvaria defect model. In the in vitro experiments, we observed that significantly more cells adhere to the serum albumin coated DBMs at every time point. In vivo bone formation with albumin coated and uncoated DBM was monitored biweekly by computed tomography until 11 weeks postoperatively while empty defects served as controls. By the seventh week, the bone defect in the albumin group was almost completely closed (remaining defect 3.0 ± 2.3%), while uncoated DBM and unfilled control groups still had significant defects (uncoated: 40.2 ± 9.1%, control: 52.4 ± 8.9%). Higher density values were also observed in the albumin coated DBM group. In addition, the serum albumin enhanced group showed significantly higher volume of newly formed bone in the microCT analysis and produced significantly higher breaking force and stiffness compared to the uncoated grafts (peak breaking force: uncoated: 15.7 ± 4 N, albumin 46.1 ± 11 N). In conclusion, this investigation shows that implanting serum albumin coated DBM significantly reduces healing period in nonhealing defects and results in mechanically stronger bone. These results also support the idea that serum albumin coating provides a convenient milieu for stem cell function, and a much improved bone grafting success can be achieved without the use of exogenous stem cells.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>25677203</pmid><doi>10.1002/jbm.b.33359</doi><tpages>7</tpages></addata></record> |
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| subjects | Adjuvants Albumin Animals Biomedical materials bone Bone Demineralization Technique Bone grafts Bone growth Bone healing Bone matrix Breaking Calvaria calvaria defect Cell Adhesion Coated Materials, Biocompatible - chemistry Coated Materials, Biocompatible - pharmacology Coating Computed tomography DBM Defects Demineralizing Extracellular Matrix - chemistry Grafts Healing In vitro methods and tests Male Materials research Materials science Osteogenesis Osteogenesis - drug effects Rats Rats, Wistar Serum Albumin Skull - injuries Skull - metabolism Skull - pathology Stem cell transplantation Stem cells Stem Cells - metabolism Stiffness Surgical implants |
| title | Serum albumin coating of demineralized bone matrix results in stronger new bone formation |
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