Gestational form of Selenium in Free-Choice Mineral Mixes Affects Transcriptome Profiles of the Neonatal Calf Testis, Including those of Steroidogenic and Spermatogenic Pathways
In areas where soils are deficient in Selenium (Se), dietary supplementation of this trace mineral directly to cattle is recommended. Because Se status affects testosterone synthesis and frequency of sperm abnormalities, and the form of Se supplemented to cows affects tissue-specific gene expression...
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| Veröffentlicht in: | Biological trace element research 2016-01, Vol.169 (1), p.56-68 |
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| creator | Cerny, K. L Garbacik, S Skees, C Burris, W. R Matthews, J. C Bridges, P. J |
| description | In areas where soils are deficient in Selenium (Se), dietary supplementation of this trace mineral directly to cattle is recommended. Because Se status affects testosterone synthesis and frequency of sperm abnormalities, and the form of Se supplemented to cows affects tissue-specific gene expression, the objective of this study was to determine whether the form of Se consumed by cows during gestation would affect the expression of mRNAs that regulate steroidogenesis and/or spermatogenesis in the neonatal calf testis. Twenty-four predominantly Angus cows were assigned randomly to have individual, ad libitum, access of a mineral mix containing 35 ppm of Se in free-choice vitamin-mineral mixes as either inorganic (ISe), organic (OSe), or a 50/50 mix of ISe and OSe (MIX), starting 4 months prior to breeding and continuing throughout gestation. Thirteen male calves were born over a 3-month period (ISe, n = 5; OSe, n = 4; MIX, n = 4), castrated within 2 days of birth, and extracted testis RNA subjected to transcriptomal analysis by microarray (Affymetrix Bovine 1.0 ST arrays) and targeted gene expression analysis by real-time reverse-transcription PCR (RT-PCR) of mRNAs encoding proteins known to affect steroidogenesis and/or spermatogenesis. The form of dam Se affected (P |
| doi_str_mv | 10.1007/s12011-015-0386-4 |
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L ; Garbacik, S ; Skees, C ; Burris, W. R ; Matthews, J. C ; Bridges, P. J</creator><creatorcontrib>Cerny, K. L ; Garbacik, S ; Skees, C ; Burris, W. R ; Matthews, J. C ; Bridges, P. J</creatorcontrib><description>In areas where soils are deficient in Selenium (Se), dietary supplementation of this trace mineral directly to cattle is recommended. Because Se status affects testosterone synthesis and frequency of sperm abnormalities, and the form of Se supplemented to cows affects tissue-specific gene expression, the objective of this study was to determine whether the form of Se consumed by cows during gestation would affect the expression of mRNAs that regulate steroidogenesis and/or spermatogenesis in the neonatal calf testis. Twenty-four predominantly Angus cows were assigned randomly to have individual, ad libitum, access of a mineral mix containing 35 ppm of Se in free-choice vitamin-mineral mixes as either inorganic (ISe), organic (OSe), or a 50/50 mix of ISe and OSe (MIX), starting 4 months prior to breeding and continuing throughout gestation. Thirteen male calves were born over a 3-month period (ISe, n = 5; OSe, n = 4; MIX, n = 4), castrated within 2 days of birth, and extracted testis RNA subjected to transcriptomal analysis by microarray (Affymetrix Bovine 1.0 ST arrays) and targeted gene expression analysis by real-time reverse-transcription PCR (RT-PCR) of mRNAs encoding proteins known to affect steroidogenesis and/or spermatogenesis. The form of dam Se affected (P < 0.05) the expression of 853 annotated genes, including 17 mRNAs putatively regulating steroidogenesis and/or spermatogenesis. Targeted RT-PCR analysis indicated that the expression of mRNA encoding proteins CYP2S1 (cytochrome P450, family 2, subfamily S, polypeptide 1), HSD17B7 (hydroxysteroid (17β) dehydrogenase 7), SULT1E1 (sulfotransferase family 1E, estrogen preferring, member 1), LDHA (lactate dehydrogenase A), CDK5R1 (cyclin-dependent kinase 5, regulatory subunit 1), and LEP (leptin) was affected (P < 0.05) by form of Se consumed by dams of developing bull calves, while AKR1C4 (aldo-keto reductase family 1, member C4) and CCND2 (cyclin D2) tended (P < 0.09) to be affected. Our results indicate that form of Se fed to dams during gestation affected the transcriptome of the neonatal calf testis. If these profiles are maintained throughout maturation, then the form of Se fed to dams may impact bull fertility and the development of Se form-dependent mineral mixes that target gestational development of the testis are warranted.</description><identifier>ISSN: 0163-4984</identifier><identifier>EISSN: 1559-0720</identifier><identifier>DOI: 10.1007/s12011-015-0386-4</identifier><identifier>PMID: 26043916</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Angus ; Animals ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; breeding ; calves ; Cattle ; cows ; Cyclin D2 - genetics ; cyclin-dependent kinase ; cyclins ; cytochrome P-450 ; Dehydrogenase ; Dietary Supplements ; Estrogens ; Female ; Fertility ; gene expression ; genes ; lactate dehydrogenase ; leptin ; Life Sciences ; Male ; males ; messenger RNA ; microarray technology ; Nutrition ; Oncology ; Oxidoreductases - genetics ; polypeptides ; Pregnancy ; Random Allocation ; reverse transcriptase polymerase chain reaction ; Selenium ; soil ; Spermatogenesis ; Spermatogenesis - genetics ; spermatozoa ; steroidogenesis ; Steroids ; testes ; Testis - metabolism ; testosterone ; transcriptome ; Transcriptome - genetics</subject><ispartof>Biological trace element research, 2016-01, Vol.169 (1), p.56-68</ispartof><rights>Springer Science+Business Media New York 2015</rights><rights>Springer Science+Business Media New York 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-182507a699c291b728c7d44eaea7dde6dc135551db1571ee1ff63f698745b5913</citedby><cites>FETCH-LOGICAL-c466t-182507a699c291b728c7d44eaea7dde6dc135551db1571ee1ff63f698745b5913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12011-015-0386-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12011-015-0386-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26043916$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cerny, K. L</creatorcontrib><creatorcontrib>Garbacik, S</creatorcontrib><creatorcontrib>Skees, C</creatorcontrib><creatorcontrib>Burris, W. R</creatorcontrib><creatorcontrib>Matthews, J. C</creatorcontrib><creatorcontrib>Bridges, P. J</creatorcontrib><title>Gestational form of Selenium in Free-Choice Mineral Mixes Affects Transcriptome Profiles of the Neonatal Calf Testis, Including those of Steroidogenic and Spermatogenic Pathways</title><title>Biological trace element research</title><addtitle>Biol Trace Elem Res</addtitle><addtitle>Biol Trace Elem Res</addtitle><description>In areas where soils are deficient in Selenium (Se), dietary supplementation of this trace mineral directly to cattle is recommended. Because Se status affects testosterone synthesis and frequency of sperm abnormalities, and the form of Se supplemented to cows affects tissue-specific gene expression, the objective of this study was to determine whether the form of Se consumed by cows during gestation would affect the expression of mRNAs that regulate steroidogenesis and/or spermatogenesis in the neonatal calf testis. Twenty-four predominantly Angus cows were assigned randomly to have individual, ad libitum, access of a mineral mix containing 35 ppm of Se in free-choice vitamin-mineral mixes as either inorganic (ISe), organic (OSe), or a 50/50 mix of ISe and OSe (MIX), starting 4 months prior to breeding and continuing throughout gestation. Thirteen male calves were born over a 3-month period (ISe, n = 5; OSe, n = 4; MIX, n = 4), castrated within 2 days of birth, and extracted testis RNA subjected to transcriptomal analysis by microarray (Affymetrix Bovine 1.0 ST arrays) and targeted gene expression analysis by real-time reverse-transcription PCR (RT-PCR) of mRNAs encoding proteins known to affect steroidogenesis and/or spermatogenesis. The form of dam Se affected (P < 0.05) the expression of 853 annotated genes, including 17 mRNAs putatively regulating steroidogenesis and/or spermatogenesis. Targeted RT-PCR analysis indicated that the expression of mRNA encoding proteins CYP2S1 (cytochrome P450, family 2, subfamily S, polypeptide 1), HSD17B7 (hydroxysteroid (17β) dehydrogenase 7), SULT1E1 (sulfotransferase family 1E, estrogen preferring, member 1), LDHA (lactate dehydrogenase A), CDK5R1 (cyclin-dependent kinase 5, regulatory subunit 1), and LEP (leptin) was affected (P < 0.05) by form of Se consumed by dams of developing bull calves, while AKR1C4 (aldo-keto reductase family 1, member C4) and CCND2 (cyclin D2) tended (P < 0.09) to be affected. Our results indicate that form of Se fed to dams during gestation affected the transcriptome of the neonatal calf testis. If these profiles are maintained throughout maturation, then the form of Se fed to dams may impact bull fertility and the development of Se form-dependent mineral mixes that target gestational development of the testis are warranted.</description><subject>Angus</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>breeding</subject><subject>calves</subject><subject>Cattle</subject><subject>cows</subject><subject>Cyclin D2 - genetics</subject><subject>cyclin-dependent kinase</subject><subject>cyclins</subject><subject>cytochrome P-450</subject><subject>Dehydrogenase</subject><subject>Dietary Supplements</subject><subject>Estrogens</subject><subject>Female</subject><subject>Fertility</subject><subject>gene expression</subject><subject>genes</subject><subject>lactate dehydrogenase</subject><subject>leptin</subject><subject>Life Sciences</subject><subject>Male</subject><subject>males</subject><subject>messenger RNA</subject><subject>microarray technology</subject><subject>Nutrition</subject><subject>Oncology</subject><subject>Oxidoreductases - genetics</subject><subject>polypeptides</subject><subject>Pregnancy</subject><subject>Random Allocation</subject><subject>reverse transcriptase polymerase chain reaction</subject><subject>Selenium</subject><subject>soil</subject><subject>Spermatogenesis</subject><subject>Spermatogenesis - genetics</subject><subject>spermatozoa</subject><subject>steroidogenesis</subject><subject>Steroids</subject><subject>testes</subject><subject>Testis - metabolism</subject><subject>testosterone</subject><subject>transcriptome</subject><subject>Transcriptome - genetics</subject><issn>0163-4984</issn><issn>1559-0720</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kc1u1DAUhSMEokPhAdiAJTYsCPgm_hkvqxEtlVqoNNO15XGuZ1wl9mAngj4Wb4hLBoRYsLrSvd85x_KpqpdA3wOl8kOGhgLUFHhN26Wo2aNqAZyrmsqGPq4WFERbM7VkJ9WznO8oBdmo9ml10gjKWgViUf24wDya0cdgeuJiGkh0ZI09Bj8NxAdynhDr1T56i-TaB0yFu_bfMZMz59COmWySCdkmfxjjgOQmRef7ci4-4x7JZyzWYxGtTO_IpqT5_I5cBttPnQ-7wsSMv0JHTNF3cVeiLTGhI-sDpsGMx82NGfffzH1-Xj1xps_44jhPq9vzj5vVp_rqy8Xl6uyqtkyIsYZlw6k0QinbKNjKZmllxxgaNLLrUHQWWs45dFvgEhDBOdE6oZaS8S1X0J5Wb2ffQ4pfp_JuPfhsse9NwDhlDZKDkEAFL-ibf9C7OKXyozMFrJGMFgpmyqaYc0KnD8kPJt1roPqhTz33qUuf-qFPzYrm1dF52g7Y_VH8LrAAzQzkcgo7TH9F_8f19SxyJmqzSz7r23WBBC28AqXan9hQtcI</recordid><startdate>20160101</startdate><enddate>20160101</enddate><creator>Cerny, K. 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L ; Garbacik, S ; Skees, C ; Burris, W. R ; Matthews, J. C ; Bridges, P. 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L</au><au>Garbacik, S</au><au>Skees, C</au><au>Burris, W. R</au><au>Matthews, J. C</au><au>Bridges, P. J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gestational form of Selenium in Free-Choice Mineral Mixes Affects Transcriptome Profiles of the Neonatal Calf Testis, Including those of Steroidogenic and Spermatogenic Pathways</atitle><jtitle>Biological trace element research</jtitle><stitle>Biol Trace Elem Res</stitle><addtitle>Biol Trace Elem Res</addtitle><date>2016-01-01</date><risdate>2016</risdate><volume>169</volume><issue>1</issue><spage>56</spage><epage>68</epage><pages>56-68</pages><issn>0163-4984</issn><eissn>1559-0720</eissn><abstract>In areas where soils are deficient in Selenium (Se), dietary supplementation of this trace mineral directly to cattle is recommended. Because Se status affects testosterone synthesis and frequency of sperm abnormalities, and the form of Se supplemented to cows affects tissue-specific gene expression, the objective of this study was to determine whether the form of Se consumed by cows during gestation would affect the expression of mRNAs that regulate steroidogenesis and/or spermatogenesis in the neonatal calf testis. Twenty-four predominantly Angus cows were assigned randomly to have individual, ad libitum, access of a mineral mix containing 35 ppm of Se in free-choice vitamin-mineral mixes as either inorganic (ISe), organic (OSe), or a 50/50 mix of ISe and OSe (MIX), starting 4 months prior to breeding and continuing throughout gestation. Thirteen male calves were born over a 3-month period (ISe, n = 5; OSe, n = 4; MIX, n = 4), castrated within 2 days of birth, and extracted testis RNA subjected to transcriptomal analysis by microarray (Affymetrix Bovine 1.0 ST arrays) and targeted gene expression analysis by real-time reverse-transcription PCR (RT-PCR) of mRNAs encoding proteins known to affect steroidogenesis and/or spermatogenesis. The form of dam Se affected (P < 0.05) the expression of 853 annotated genes, including 17 mRNAs putatively regulating steroidogenesis and/or spermatogenesis. Targeted RT-PCR analysis indicated that the expression of mRNA encoding proteins CYP2S1 (cytochrome P450, family 2, subfamily S, polypeptide 1), HSD17B7 (hydroxysteroid (17β) dehydrogenase 7), SULT1E1 (sulfotransferase family 1E, estrogen preferring, member 1), LDHA (lactate dehydrogenase A), CDK5R1 (cyclin-dependent kinase 5, regulatory subunit 1), and LEP (leptin) was affected (P < 0.05) by form of Se consumed by dams of developing bull calves, while AKR1C4 (aldo-keto reductase family 1, member C4) and CCND2 (cyclin D2) tended (P < 0.09) to be affected. Our results indicate that form of Se fed to dams during gestation affected the transcriptome of the neonatal calf testis. If these profiles are maintained throughout maturation, then the form of Se fed to dams may impact bull fertility and the development of Se form-dependent mineral mixes that target gestational development of the testis are warranted.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>26043916</pmid><doi>10.1007/s12011-015-0386-4</doi><tpages>13</tpages></addata></record> |
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| subjects | Angus Animals Biochemistry Biomedical and Life Sciences Biotechnology breeding calves Cattle cows Cyclin D2 - genetics cyclin-dependent kinase cyclins cytochrome P-450 Dehydrogenase Dietary Supplements Estrogens Female Fertility gene expression genes lactate dehydrogenase leptin Life Sciences Male males messenger RNA microarray technology Nutrition Oncology Oxidoreductases - genetics polypeptides Pregnancy Random Allocation reverse transcriptase polymerase chain reaction Selenium soil Spermatogenesis Spermatogenesis - genetics spermatozoa steroidogenesis Steroids testes Testis - metabolism testosterone transcriptome Transcriptome - genetics |
| title | Gestational form of Selenium in Free-Choice Mineral Mixes Affects Transcriptome Profiles of the Neonatal Calf Testis, Including those of Steroidogenic and Spermatogenic Pathways |
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