Synthesis and in vitro antifungal efficacy of oleoyl-chitosan nanoparticles against plant pathogenic fungi
An antifungal dispersion system was prepared by oleoyl-chitosan (O-chitosan) nanoparticles, and the antifungal activity against several plant pathogenic fungi was investigated. Under scanning electron microscopy, the nanoparticles formulation appeared to be uniform with almost spherical shape. The p...
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| Veröffentlicht in: | International journal of biological macromolecules 2016-01, Vol.82, p.830-836 |
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| container_title | International journal of biological macromolecules |
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| creator | Xing, Ke Shen, Xiaoqiang Zhu, Xiao Ju, Xiuyun Miao, Xiangmin Tian, Jun Feng, Zhaozhong Peng, Xue Jiang, Jihong Qin, Sheng |
| description | An antifungal dispersion system was prepared by oleoyl-chitosan (O-chitosan) nanoparticles, and the antifungal activity against several plant pathogenic fungi was investigated. Under scanning electron microscopy, the nanoparticles formulation appeared to be uniform with almost spherical shape. The particle size of nanoparticles was around 296.962nm. Transmission electron microscopy observation showed that nanoparticles could be well distributed in potato dextrose agar medium. Mycelium growth experiment demonstrated that Nigrospora sphaerica, Botryosphaeria dothidea, Nigrospora oryzae and Alternaria tenuissima were chitosan-sensitive, while Gibberella zeae and Fusarium culmorum were chitosan-resistant. The antifungal index was increased as the concentration of nanoparticles increased for chitosan-sensitive fungi. Fatty acid analyses revealed that plasma membranes of chitosan-sensitive fungi were shown to have lower levels of unsaturated fatty acid than chitosan-resistant fungi. Phylogenetic analysis based on ITS gene sequences indicated that two chitosan-resistant fungi had a near phylogenetic relationship. Results showed that O-chitosan nanoparticles could be a useful alternative for controlling pathogenic fungi in agriculture. |
| doi_str_mv | 10.1016/j.ijbiomac.2015.09.074 |
| format | Article |
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Under scanning electron microscopy, the nanoparticles formulation appeared to be uniform with almost spherical shape. The particle size of nanoparticles was around 296.962nm. Transmission electron microscopy observation showed that nanoparticles could be well distributed in potato dextrose agar medium. Mycelium growth experiment demonstrated that Nigrospora sphaerica, Botryosphaeria dothidea, Nigrospora oryzae and Alternaria tenuissima were chitosan-sensitive, while Gibberella zeae and Fusarium culmorum were chitosan-resistant. The antifungal index was increased as the concentration of nanoparticles increased for chitosan-sensitive fungi. Fatty acid analyses revealed that plasma membranes of chitosan-sensitive fungi were shown to have lower levels of unsaturated fatty acid than chitosan-resistant fungi. Phylogenetic analysis based on ITS gene sequences indicated that two chitosan-resistant fungi had a near phylogenetic relationship. 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All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-deef0f4ebaab3f2e6f47d9ffbab5fc56ce4c631242d69aec231e565773c30d173</citedby><cites>FETCH-LOGICAL-c434t-deef0f4ebaab3f2e6f47d9ffbab5fc56ce4c631242d69aec231e565773c30d173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijbiomac.2015.09.074$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26434526$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xing, Ke</creatorcontrib><creatorcontrib>Shen, Xiaoqiang</creatorcontrib><creatorcontrib>Zhu, Xiao</creatorcontrib><creatorcontrib>Ju, Xiuyun</creatorcontrib><creatorcontrib>Miao, Xiangmin</creatorcontrib><creatorcontrib>Tian, Jun</creatorcontrib><creatorcontrib>Feng, Zhaozhong</creatorcontrib><creatorcontrib>Peng, Xue</creatorcontrib><creatorcontrib>Jiang, Jihong</creatorcontrib><creatorcontrib>Qin, Sheng</creatorcontrib><title>Synthesis and in vitro antifungal efficacy of oleoyl-chitosan nanoparticles against plant pathogenic fungi</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>An antifungal dispersion system was prepared by oleoyl-chitosan (O-chitosan) nanoparticles, and the antifungal activity against several plant pathogenic fungi was investigated. Under scanning electron microscopy, the nanoparticles formulation appeared to be uniform with almost spherical shape. The particle size of nanoparticles was around 296.962nm. Transmission electron microscopy observation showed that nanoparticles could be well distributed in potato dextrose agar medium. Mycelium growth experiment demonstrated that Nigrospora sphaerica, Botryosphaeria dothidea, Nigrospora oryzae and Alternaria tenuissima were chitosan-sensitive, while Gibberella zeae and Fusarium culmorum were chitosan-resistant. The antifungal index was increased as the concentration of nanoparticles increased for chitosan-sensitive fungi. Fatty acid analyses revealed that plasma membranes of chitosan-sensitive fungi were shown to have lower levels of unsaturated fatty acid than chitosan-resistant fungi. Phylogenetic analysis based on ITS gene sequences indicated that two chitosan-resistant fungi had a near phylogenetic relationship. Results showed that O-chitosan nanoparticles could be a useful alternative for controlling pathogenic fungi in agriculture.</description><subject>Antifungal activity</subject><subject>Antifungal Agents - chemistry</subject><subject>Antifungal Agents - pharmacology</subject><subject>Chitosan</subject><subject>Chitosan - chemistry</subject><subject>Chitosan - pharmacology</subject><subject>DNA, Intergenic</subject><subject>Fatty Acids - chemistry</subject><subject>Fatty Acids - metabolism</subject><subject>Fungi - classification</subject><subject>Fungi - drug effects</subject><subject>Fungi - genetics</subject><subject>Fungi - metabolism</subject><subject>Microbial Sensitivity Tests</subject><subject>Mycelium - drug effects</subject><subject>Nanoparticles - chemistry</subject><subject>Nanoparticles - ultrastructure</subject><subject>Particle Size</subject><subject>Phylogeny</subject><subject>Plant Diseases - microbiology</subject><subject>Plant pathogenic fungi</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtu2zAQRYkiQeM8fiHgshsppEhR1q5FkD6AAF00WRMUNbRHkEmVpAP470PDSbZZDWZw752ZQ8gtZzVnXN1NNU4Dhp2xdcN4W7O-Zp38QlZ83fUVY0yckRXjkldrLtgFuUxpKlPV8vVXctEoKWTbqBWZ_h183kLCRI0fKXr6gjmG0mR0e78xMwXn0Bp7oMHRMEM4zJXdYg7JeOqND4uJGe0MJWFj0KdMl7nY6WLyNmzAo6XHJLwm587MCW7e6hV5_vnwdP-7evz768_9j8fKlqNyNQI45iQMxgzCNaCc7MbeucEMrbOtsiCtEryRzah6A7YRHFrVdp2wgo28E1fk2yl3ieH_HlLWO0wW5nIUhH3SvGu56phUTZGqk9TGkFIEp5eIOxMPmjN95Kwn_c5ZHzlr1uvCuRhv33bshx2MH7Z3sEXw_SSA8ukLQtTJIngLI0awWY8BP9vxCii_lXI</recordid><startdate>201601</startdate><enddate>201601</enddate><creator>Xing, Ke</creator><creator>Shen, Xiaoqiang</creator><creator>Zhu, Xiao</creator><creator>Ju, Xiuyun</creator><creator>Miao, Xiangmin</creator><creator>Tian, Jun</creator><creator>Feng, Zhaozhong</creator><creator>Peng, Xue</creator><creator>Jiang, Jihong</creator><creator>Qin, Sheng</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201601</creationdate><title>Synthesis and in vitro antifungal efficacy of oleoyl-chitosan nanoparticles against plant pathogenic fungi</title><author>Xing, Ke ; 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Under scanning electron microscopy, the nanoparticles formulation appeared to be uniform with almost spherical shape. The particle size of nanoparticles was around 296.962nm. Transmission electron microscopy observation showed that nanoparticles could be well distributed in potato dextrose agar medium. Mycelium growth experiment demonstrated that Nigrospora sphaerica, Botryosphaeria dothidea, Nigrospora oryzae and Alternaria tenuissima were chitosan-sensitive, while Gibberella zeae and Fusarium culmorum were chitosan-resistant. The antifungal index was increased as the concentration of nanoparticles increased for chitosan-sensitive fungi. Fatty acid analyses revealed that plasma membranes of chitosan-sensitive fungi were shown to have lower levels of unsaturated fatty acid than chitosan-resistant fungi. Phylogenetic analysis based on ITS gene sequences indicated that two chitosan-resistant fungi had a near phylogenetic relationship. 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| subjects | Antifungal activity Antifungal Agents - chemistry Antifungal Agents - pharmacology Chitosan Chitosan - chemistry Chitosan - pharmacology DNA, Intergenic Fatty Acids - chemistry Fatty Acids - metabolism Fungi - classification Fungi - drug effects Fungi - genetics Fungi - metabolism Microbial Sensitivity Tests Mycelium - drug effects Nanoparticles - chemistry Nanoparticles - ultrastructure Particle Size Phylogeny Plant Diseases - microbiology Plant pathogenic fungi |
| title | Synthesis and in vitro antifungal efficacy of oleoyl-chitosan nanoparticles against plant pathogenic fungi |
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