Characterization and gene cloning of monoclonal antibody specific for the hepatitis B virus X protein
The hepatis B virus X protein (HBx) has been thought to be implicated in the development of hepatocellular carcinoma. Although many functions of HBx have been reported, it is not clear which of HBx functions is important in hepatocellular carcinogenesis. To study HBx function, we produced a monoclon...
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| Veröffentlicht in: | Hybridoma 2000-02, Vol.19 (1), p.73-80 |
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| creator | OG YI PARK YOUNG HEE JIN LEE, M SHIN, H.-J KIM, H.-I CHO, H YUN, C.-W JUNG KOO YOUN PARK, S |
| description | The hepatis B virus X protein (HBx) has been thought to be implicated in the development of hepatocellular carcinoma. Although many functions of HBx have been reported, it is not clear which of HBx functions is important in hepatocellular carcinogenesis. To study HBx function, we produced a monoclonal anti-HBx Ab secreted by hybridoma cell clone H7 and mapped its epitope to a region of HBx between amino acids 29 and 48 by Western blot with truncated forms of HBx and by enzyme-linked immunoadsorbent assay (ELISA) with synthetic HBx peptides. The variable regions of H7 anti-HBx Ab were cloned by polymerase chain reaction using the degenerate-primers and by the 5' rapid amplification-cDNA end method. The sequence analyses revealed that the variable gene segments of the heavy and light chains are the members of mouse heavy chain variable gene 1 family and kappa light chain variable gene 2 family, respectively. In addition, J(H)2 or Jkappa4 gene segment at the end of the heavy-chain or light-chain variable region and DSP2.x gene segment in the CDR 3 of heavy chain were identified. |
| doi_str_mv | 10.1089/027245700315815 |
| format | Article |
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Although many functions of HBx have been reported, it is not clear which of HBx functions is important in hepatocellular carcinogenesis. To study HBx function, we produced a monoclonal anti-HBx Ab secreted by hybridoma cell clone H7 and mapped its epitope to a region of HBx between amino acids 29 and 48 by Western blot with truncated forms of HBx and by enzyme-linked immunoadsorbent assay (ELISA) with synthetic HBx peptides. The variable regions of H7 anti-HBx Ab were cloned by polymerase chain reaction using the degenerate-primers and by the 5' rapid amplification-cDNA end method. The sequence analyses revealed that the variable gene segments of the heavy and light chains are the members of mouse heavy chain variable gene 1 family and kappa light chain variable gene 2 family, respectively. In addition, J(H)2 or Jkappa4 gene segment at the end of the heavy-chain or light-chain variable region and DSP2.x gene segment in the CDR 3 of heavy chain were identified.</description><identifier>ISSN: 0272-457X</identifier><identifier>EISSN: 2168-7897</identifier><identifier>EISSN: 0272-457X</identifier><identifier>DOI: 10.1089/027245700315815</identifier><identifier>PMID: 10768843</identifier><identifier>CODEN: HYBRDY</identifier><language>eng</language><publisher>Larchmont, NY: Liebert</publisher><subject>Amino Acid Sequence ; Animals ; Antibodies, Monoclonal - biosynthesis ; Antibodies, Monoclonal - chemistry ; Antibodies, Monoclonal - genetics ; Antibody Specificity ; Antigen-Antibody Reactions ; AX protein ; Base Sequence ; Biological and medical sciences ; Biotechnology ; Cloning, Molecular ; Epitope Mapping ; Fundamental and applied biological sciences. Psychology ; Health. Pharmaceutical industry ; Hepatitis B Antibodies - biosynthesis ; Hepatitis B Antibodies - chemistry ; Hepatitis B Antibodies - genetics ; Hepatitis B virus ; Hybridomas ; Immunoglobulin Variable Region - genetics ; Industrial applications and implications. Economical aspects ; Liver - immunology ; Liver - metabolism ; Male ; Mice ; Mice, Inbred BALB C ; Molecular Sequence Data ; Monoclonal antibodies ; Production of active biomolecules ; Trans-Activators - immunology ; X protein</subject><ispartof>Hybridoma, 2000-02, Vol.19 (1), p.73-80</ispartof><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-ec344a640f008a6a9ed22c5c79d9645813ab3fc76fe7cc18fac1f4960938b903</citedby><cites>FETCH-LOGICAL-c353t-ec344a640f008a6a9ed22c5c79d9645813ab3fc76fe7cc18fac1f4960938b903</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3040,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1326897$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10768843$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>OG YI PARK</creatorcontrib><creatorcontrib>YOUNG HEE JIN</creatorcontrib><creatorcontrib>LEE, M</creatorcontrib><creatorcontrib>SHIN, H.-J</creatorcontrib><creatorcontrib>KIM, H.-I</creatorcontrib><creatorcontrib>CHO, H</creatorcontrib><creatorcontrib>YUN, C.-W</creatorcontrib><creatorcontrib>JUNG KOO YOUN</creatorcontrib><creatorcontrib>PARK, S</creatorcontrib><title>Characterization and gene cloning of monoclonal antibody specific for the hepatitis B virus X protein</title><title>Hybridoma</title><addtitle>Hybridoma</addtitle><description>The hepatis B virus X protein (HBx) has been thought to be implicated in the development of hepatocellular carcinoma. Although many functions of HBx have been reported, it is not clear which of HBx functions is important in hepatocellular carcinogenesis. To study HBx function, we produced a monoclonal anti-HBx Ab secreted by hybridoma cell clone H7 and mapped its epitope to a region of HBx between amino acids 29 and 48 by Western blot with truncated forms of HBx and by enzyme-linked immunoadsorbent assay (ELISA) with synthetic HBx peptides. The variable regions of H7 anti-HBx Ab were cloned by polymerase chain reaction using the degenerate-primers and by the 5' rapid amplification-cDNA end method. The sequence analyses revealed that the variable gene segments of the heavy and light chains are the members of mouse heavy chain variable gene 1 family and kappa light chain variable gene 2 family, respectively. In addition, J(H)2 or Jkappa4 gene segment at the end of the heavy-chain or light-chain variable region and DSP2.x gene segment in the CDR 3 of heavy chain were identified.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Monoclonal - biosynthesis</subject><subject>Antibodies, Monoclonal - chemistry</subject><subject>Antibodies, Monoclonal - genetics</subject><subject>Antibody Specificity</subject><subject>Antigen-Antibody Reactions</subject><subject>AX protein</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cloning, Molecular</subject><subject>Epitope Mapping</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Health. Pharmaceutical industry</subject><subject>Hepatitis B Antibodies - biosynthesis</subject><subject>Hepatitis B Antibodies - chemistry</subject><subject>Hepatitis B Antibodies - genetics</subject><subject>Hepatitis B virus</subject><subject>Hybridomas</subject><subject>Immunoglobulin Variable Region - genetics</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Liver - immunology</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Molecular Sequence Data</subject><subject>Monoclonal antibodies</subject><subject>Production of active biomolecules</subject><subject>Trans-Activators - immunology</subject><subject>X protein</subject><issn>0272-457X</issn><issn>2168-7897</issn><issn>0272-457X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc1LAzEQxYMotlbP3iQH8bY22ewm2aMWv6DgpYfeljQ7aSO7yZpshfrXm9KC4mkY3m_eMG8QuqbknhJZTUku8qIUhDBaSlqeoHFOucyErMQpGu_VLMnLEbqI8YMQUuaFOEcjSgSXsmBjBLONCkoPEOy3Gqx3WLkGr8EB1q131q2xN7jzzu9b1SZ5sCvf7HDsQVtjNTY-4GEDeAN9chhsxI_4y4ZtxEvcBz-AdZfozKg2wtWxTtDi-Wkxe83m7y9vs4d5plnJhgw0KwrFC2IIkYqrCpo816UWVVPxIh3I1IoZLbgBoTWVRmlqioqTislVRdgE3R1s09rPLcSh7mzU0LbKgd_GmoqScsZ5AqcHUAcfYwBT98F2KuxqSup9sPW_YNPEzdF6u-qg-cMfkkzA7RFQUavWBOW0jb8cy3l6CvsBPxGAow</recordid><startdate>20000201</startdate><enddate>20000201</enddate><creator>OG YI PARK</creator><creator>YOUNG HEE JIN</creator><creator>LEE, M</creator><creator>SHIN, H.-J</creator><creator>KIM, H.-I</creator><creator>CHO, H</creator><creator>YUN, C.-W</creator><creator>JUNG KOO YOUN</creator><creator>PARK, S</creator><general>Liebert</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>20000201</creationdate><title>Characterization and gene cloning of monoclonal antibody specific for the hepatitis B virus X protein</title><author>OG YI PARK ; YOUNG HEE JIN ; LEE, M ; SHIN, H.-J ; KIM, H.-I ; CHO, H ; YUN, C.-W ; JUNG KOO YOUN ; PARK, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-ec344a640f008a6a9ed22c5c79d9645813ab3fc76fe7cc18fac1f4960938b903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies, Monoclonal - biosynthesis</topic><topic>Antibodies, Monoclonal - chemistry</topic><topic>Antibodies, Monoclonal - genetics</topic><topic>Antibody Specificity</topic><topic>Antigen-Antibody Reactions</topic><topic>AX protein</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cloning, Molecular</topic><topic>Epitope Mapping</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Health. Pharmaceutical industry</topic><topic>Hepatitis B Antibodies - biosynthesis</topic><topic>Hepatitis B Antibodies - chemistry</topic><topic>Hepatitis B Antibodies - genetics</topic><topic>Hepatitis B virus</topic><topic>Hybridomas</topic><topic>Immunoglobulin Variable Region - genetics</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Liver - immunology</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Molecular Sequence Data</topic><topic>Monoclonal antibodies</topic><topic>Production of active biomolecules</topic><topic>Trans-Activators - immunology</topic><topic>X protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>OG YI PARK</creatorcontrib><creatorcontrib>YOUNG HEE JIN</creatorcontrib><creatorcontrib>LEE, M</creatorcontrib><creatorcontrib>SHIN, H.-J</creatorcontrib><creatorcontrib>KIM, H.-I</creatorcontrib><creatorcontrib>CHO, H</creatorcontrib><creatorcontrib>YUN, C.-W</creatorcontrib><creatorcontrib>JUNG KOO YOUN</creatorcontrib><creatorcontrib>PARK, S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Hybridoma</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>OG YI PARK</au><au>YOUNG HEE JIN</au><au>LEE, M</au><au>SHIN, H.-J</au><au>KIM, H.-I</au><au>CHO, H</au><au>YUN, C.-W</au><au>JUNG KOO YOUN</au><au>PARK, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization and gene cloning of monoclonal antibody specific for the hepatitis B virus X protein</atitle><jtitle>Hybridoma</jtitle><addtitle>Hybridoma</addtitle><date>2000-02-01</date><risdate>2000</risdate><volume>19</volume><issue>1</issue><spage>73</spage><epage>80</epage><pages>73-80</pages><issn>0272-457X</issn><eissn>2168-7897</eissn><eissn>0272-457X</eissn><coden>HYBRDY</coden><abstract>The hepatis B virus X protein (HBx) has been thought to be implicated in the development of hepatocellular carcinoma. Although many functions of HBx have been reported, it is not clear which of HBx functions is important in hepatocellular carcinogenesis. To study HBx function, we produced a monoclonal anti-HBx Ab secreted by hybridoma cell clone H7 and mapped its epitope to a region of HBx between amino acids 29 and 48 by Western blot with truncated forms of HBx and by enzyme-linked immunoadsorbent assay (ELISA) with synthetic HBx peptides. The variable regions of H7 anti-HBx Ab were cloned by polymerase chain reaction using the degenerate-primers and by the 5' rapid amplification-cDNA end method. The sequence analyses revealed that the variable gene segments of the heavy and light chains are the members of mouse heavy chain variable gene 1 family and kappa light chain variable gene 2 family, respectively. In addition, J(H)2 or Jkappa4 gene segment at the end of the heavy-chain or light-chain variable region and DSP2.x gene segment in the CDR 3 of heavy chain were identified.</abstract><cop>Larchmont, NY</cop><pub>Liebert</pub><pmid>10768843</pmid><doi>10.1089/027245700315815</doi><tpages>8</tpages></addata></record> |
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| subjects | Amino Acid Sequence Animals Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - genetics Antibody Specificity Antigen-Antibody Reactions AX protein Base Sequence Biological and medical sciences Biotechnology Cloning, Molecular Epitope Mapping Fundamental and applied biological sciences. Psychology Health. Pharmaceutical industry Hepatitis B Antibodies - biosynthesis Hepatitis B Antibodies - chemistry Hepatitis B Antibodies - genetics Hepatitis B virus Hybridomas Immunoglobulin Variable Region - genetics Industrial applications and implications. Economical aspects Liver - immunology Liver - metabolism Male Mice Mice, Inbred BALB C Molecular Sequence Data Monoclonal antibodies Production of active biomolecules Trans-Activators - immunology X protein |
| title | Characterization and gene cloning of monoclonal antibody specific for the hepatitis B virus X protein |
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