Use of cDNA microarrays to probe and understand the toxicological consequences of altered gene expression
Genomic sciences offer the ability to measure quantitative modulation of transcription in cells and tissues under a wide variety of conditions. We have developed a series of custom cDNA microarrays specifically to investigate toxicity processes. Around 600 marker genes for toxicity were selected and...
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| Veröffentlicht in: | Toxicology letters 2000-03, Vol.112 (1-3), p.473-477 |
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| container_end_page | 477 |
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| container_title | Toxicology letters |
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| creator | Pennie, William D. |
| description | Genomic sciences offer the ability to measure quantitative modulation of transcription in cells and tissues under a wide variety of conditions. We have developed a series of custom cDNA microarrays specifically to investigate toxicity processes. Around 600 marker genes for toxicity were selected and representative cDNA clones were obtained, amplified and purified by polymerase chain reaction (PCR), before being immobilised on nylon membranes. A detailed database on biochemical function, role in disease and allelic variation has been assembled for each gene. Applications in our laboratory include mechanistic investigation of a number of toxic endpoints such as hepatotoxicity and endocrine disruption. |
| doi_str_mv | 10.1016/S0378-4274(99)00199-X |
| format | Article |
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| ispartof | Toxicology letters, 2000-03, Vol.112 (1-3), p.473-477 |
| issn | 0378-4274 1879-3169 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Databases, Factual DNA, Complementary - genetics Endocrine disruption Gene Expression Regulation Gene regulation Genetic Markers Genomics Hepatotoxicity Humans In vitro testing micromethods Oligonucleotide Array Sequence Analysis Polymerase Chain Reaction Toxicology - methods Transcription, Genetic |
| title | Use of cDNA microarrays to probe and understand the toxicological consequences of altered gene expression |
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