Effect of differentiation on the regulation of indole alkaloid production in Catharanthus roseus hairy roots
In vitro cultures of hairy root derived from Catharanthus roseus accumulate higher levels of indole alkaloids than cell suspension cultures. Hairy roots were interconverted to undifferentiated cells by manipulation of the culture medium. When the concentration of micronutrients in the culture medium...
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| Veröffentlicht in: | Plant cell reports 1998-11, Vol.18 (1/2), p.99-104 |
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| creator | Moreno-Valenzuela, O.A Galaz-Avalos, R.M Minero-Garcia, Y Loyola-Vargas, V.M |
| description | In vitro cultures of hairy root derived from Catharanthus roseus accumulate higher levels of indole alkaloids than cell suspension cultures. Hairy roots were interconverted to undifferentiated cells by manipulation of the culture medium. When the concentration of micronutrients in the culture medium was five times that of Phillips and Collins (1979) medium, cell suspensions formed from the hairy roots. The alkaloid content was five times lower in the cell suspensions than in the control, but upon regeneration of the roots the alkaloid content regained its original level. The formation of cell suspensions from hairy roots was also accompanied by a reduction in tryptophan decarboxylase and the strictosidine synthase activity to less than 5% and 30%, respectively. 3-Hydroxymethylglutaryl coenzyme A reductase activity was the same in the cell suspension and in the regenerated line. |
| doi_str_mv | 10.1007/s002990050539 |
| format | Article |
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Hairy roots were interconverted to undifferentiated cells by manipulation of the culture medium. When the concentration of micronutrients in the culture medium was five times that of Phillips and Collins (1979) medium, cell suspensions formed from the hairy roots. The alkaloid content was five times lower in the cell suspensions than in the control, but upon regeneration of the roots the alkaloid content regained its original level. The formation of cell suspensions from hairy roots was also accompanied by a reduction in tryptophan decarboxylase and the strictosidine synthase activity to less than 5% and 30%, respectively. 3-Hydroxymethylglutaryl coenzyme A reductase activity was the same in the cell suspension and in the regenerated line.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s002990050539</identifier><identifier>CODEN: PCRPD8</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Alkaloids ; Bacteria ; Biological and medical sciences ; biosynthesis ; Biotechnology ; carboxy-lyases ; Catharanthus roseus ; Cell culture ; cell differentiation ; cell suspension culture ; Cell suspensions ; Coenzyme A ; culture media ; developmental stages ; enzyme activity ; Establishment of new cell lines, improvement of cultural methods, mass culture ; Eukaryotic cell cultures ; Fundamental and applied biological sciences. Psychology ; genetic transformation ; Hairy root ; Hydroxymethylglutaryl-CoA reductase ; in vitro culture ; indole alkaloids ; Indoles ; Methods. Procedures. Technologies ; Micronutrients ; pathogenesis ; phillips and collins medium ; plant anatomy ; Plant cells and fungal cells ; Regeneration ; regenerative ability ; regulation ; Rhizobium rhizogenes ; Roots ; secondary metabolites ; Strictosidine synthase ; timing ; Tryptophan</subject><ispartof>Plant cell reports, 1998-11, Vol.18 (1/2), p.99-104</ispartof><rights>1999 INIST-CNRS</rights><rights>Plant Cell Reports is a copyright of Springer, (1998). All Rights Reserved.</rights><rights>Springer-Verlag Berlin Heidelberg 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c374t-3e8fa044203e2fb1b8ff46620bd65eca61b2443737a186abebac0318f978de553</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1695884$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Moreno-Valenzuela, O.A</creatorcontrib><creatorcontrib>Galaz-Avalos, R.M</creatorcontrib><creatorcontrib>Minero-Garcia, Y</creatorcontrib><creatorcontrib>Loyola-Vargas, V.M</creatorcontrib><title>Effect of differentiation on the regulation of indole alkaloid production in Catharanthus roseus hairy roots</title><title>Plant cell reports</title><description>In vitro cultures of hairy root derived from Catharanthus roseus accumulate higher levels of indole alkaloids than cell suspension cultures. Hairy roots were interconverted to undifferentiated cells by manipulation of the culture medium. When the concentration of micronutrients in the culture medium was five times that of Phillips and Collins (1979) medium, cell suspensions formed from the hairy roots. The alkaloid content was five times lower in the cell suspensions than in the control, but upon regeneration of the roots the alkaloid content regained its original level. The formation of cell suspensions from hairy roots was also accompanied by a reduction in tryptophan decarboxylase and the strictosidine synthase activity to less than 5% and 30%, respectively. 3-Hydroxymethylglutaryl coenzyme A reductase activity was the same in the cell suspension and in the regenerated line.</description><subject>Alkaloids</subject><subject>Bacteria</subject><subject>Biological and medical sciences</subject><subject>biosynthesis</subject><subject>Biotechnology</subject><subject>carboxy-lyases</subject><subject>Catharanthus roseus</subject><subject>Cell culture</subject><subject>cell differentiation</subject><subject>cell suspension culture</subject><subject>Cell suspensions</subject><subject>Coenzyme A</subject><subject>culture media</subject><subject>developmental stages</subject><subject>enzyme activity</subject><subject>Establishment of new cell lines, improvement of cultural methods, mass culture</subject><subject>Eukaryotic cell cultures</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genetic transformation</subject><subject>Hairy root</subject><subject>Hydroxymethylglutaryl-CoA reductase</subject><subject>in vitro culture</subject><subject>indole alkaloids</subject><subject>Indoles</subject><subject>Methods. Procedures. Technologies</subject><subject>Micronutrients</subject><subject>pathogenesis</subject><subject>phillips and collins medium</subject><subject>plant anatomy</subject><subject>Plant cells and fungal cells</subject><subject>Regeneration</subject><subject>regenerative ability</subject><subject>regulation</subject><subject>Rhizobium rhizogenes</subject><subject>Roots</subject><subject>secondary metabolites</subject><subject>Strictosidine synthase</subject><subject>timing</subject><subject>Tryptophan</subject><issn>0721-7714</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kc9rFTEQgINY8Nl69GxQ6W3r5Ncme5RHrUKhBy14W2azSV9quqlJ9tD_3jzfA1EQApNkvhm-ZAh5zeCCAegPBYAPA4ACJYZnZMOk4B0H8f052YDmrNOayRfkZSn3AC2p-w2Jl947W2nydA5tm91SA9aQFtpW3Tma3d0ajzeehmVO0VGMPzCmMNPHnObV_s6GhW6x7jDjUndroTkV18IOQ35qh1TLGTnxGIt7dYyn5PbT5bft5-765urL9uN1Z4WWtRPOeAQpm7rjfmKT8V72PYdp7pWz2LOJy6YvNDLT4-QmtCCY8YM2s1NKnJLzQ99m93N1pY4PoVgXIy4urWVkWjHFhz349h_wPq15aW6jMdIo0cs99O5_EOesUcoMplHdgbLt3SU7Pz7m8ID5aWQw7scz_jWexr8_dsViMfr2azaUP0X9oJpCw94cMI9pxLvckNuvHJgAboa9n_gFi1-YdA</recordid><startdate>19981101</startdate><enddate>19981101</enddate><creator>Moreno-Valenzuela, O.A</creator><creator>Galaz-Avalos, R.M</creator><creator>Minero-Garcia, Y</creator><creator>Loyola-Vargas, V.M</creator><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7QO</scope></search><sort><creationdate>19981101</creationdate><title>Effect of differentiation on the regulation of indole alkaloid production in Catharanthus roseus hairy roots</title><author>Moreno-Valenzuela, O.A ; Galaz-Avalos, R.M ; Minero-Garcia, Y ; Loyola-Vargas, V.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c374t-3e8fa044203e2fb1b8ff46620bd65eca61b2443737a186abebac0318f978de553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Alkaloids</topic><topic>Bacteria</topic><topic>Biological and medical sciences</topic><topic>biosynthesis</topic><topic>Biotechnology</topic><topic>carboxy-lyases</topic><topic>Catharanthus roseus</topic><topic>Cell culture</topic><topic>cell differentiation</topic><topic>cell suspension culture</topic><topic>Cell suspensions</topic><topic>Coenzyme A</topic><topic>culture media</topic><topic>developmental stages</topic><topic>enzyme activity</topic><topic>Establishment of new cell lines, improvement of cultural methods, mass culture</topic><topic>Eukaryotic cell cultures</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genetic transformation</topic><topic>Hairy root</topic><topic>Hydroxymethylglutaryl-CoA reductase</topic><topic>in vitro culture</topic><topic>indole alkaloids</topic><topic>Indoles</topic><topic>Methods. Procedures. Technologies</topic><topic>Micronutrients</topic><topic>pathogenesis</topic><topic>phillips and collins medium</topic><topic>plant anatomy</topic><topic>Plant cells and fungal cells</topic><topic>Regeneration</topic><topic>regenerative ability</topic><topic>regulation</topic><topic>Rhizobium rhizogenes</topic><topic>Roots</topic><topic>secondary metabolites</topic><topic>Strictosidine synthase</topic><topic>timing</topic><topic>Tryptophan</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moreno-Valenzuela, O.A</creatorcontrib><creatorcontrib>Galaz-Avalos, R.M</creatorcontrib><creatorcontrib>Minero-Garcia, Y</creatorcontrib><creatorcontrib>Loyola-Vargas, V.M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moreno-Valenzuela, O.A</au><au>Galaz-Avalos, R.M</au><au>Minero-Garcia, Y</au><au>Loyola-Vargas, V.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of differentiation on the regulation of indole alkaloid production in Catharanthus roseus hairy roots</atitle><jtitle>Plant cell reports</jtitle><date>1998-11-01</date><risdate>1998</risdate><volume>18</volume><issue>1/2</issue><spage>99</spage><epage>104</epage><pages>99-104</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><coden>PCRPD8</coden><abstract>In vitro cultures of hairy root derived from Catharanthus roseus accumulate higher levels of indole alkaloids than cell suspension cultures. Hairy roots were interconverted to undifferentiated cells by manipulation of the culture medium. When the concentration of micronutrients in the culture medium was five times that of Phillips and Collins (1979) medium, cell suspensions formed from the hairy roots. The alkaloid content was five times lower in the cell suspensions than in the control, but upon regeneration of the roots the alkaloid content regained its original level. The formation of cell suspensions from hairy roots was also accompanied by a reduction in tryptophan decarboxylase and the strictosidine synthase activity to less than 5% and 30%, respectively. 3-Hydroxymethylglutaryl coenzyme A reductase activity was the same in the cell suspension and in the regenerated line.</abstract><cop>Berlin</cop><pub>Springer</pub><doi>10.1007/s002990050539</doi><tpages>6</tpages></addata></record> |
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| ispartof | Plant cell reports, 1998-11, Vol.18 (1/2), p.99-104 |
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| subjects | Alkaloids Bacteria Biological and medical sciences biosynthesis Biotechnology carboxy-lyases Catharanthus roseus Cell culture cell differentiation cell suspension culture Cell suspensions Coenzyme A culture media developmental stages enzyme activity Establishment of new cell lines, improvement of cultural methods, mass culture Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology genetic transformation Hairy root Hydroxymethylglutaryl-CoA reductase in vitro culture indole alkaloids Indoles Methods. Procedures. Technologies Micronutrients pathogenesis phillips and collins medium plant anatomy Plant cells and fungal cells Regeneration regenerative ability regulation Rhizobium rhizogenes Roots secondary metabolites Strictosidine synthase timing Tryptophan |
| title | Effect of differentiation on the regulation of indole alkaloid production in Catharanthus roseus hairy roots |
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