Impact of Q139R substitution of MEB4-Cry2Aa toxin on its stability, accessibility and toxicity against Ephestia kuehniella

The Bacillus thuringiensis subsp. kurstaki strain MEB4 was previously found to be highly toxic to Ephestia kuehniella. SDS-PAGE analysis of the recombinant strain DH5α (pBS-cry2Aa-MEB4) showed that Cry2Aa-MEB4 delta-endotoxins were forming inclusion bodies, and were 2.75 fold more toxic towards E. k...

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Veröffentlicht in:	International journal of biological macromolecules 2015-11, Vol.81, p.701-709
Hauptverfasser:	Nouha, Abdelmalek, Sameh, Sellami, Fakher, Frikha, Slim, Tounsi, Souad, Rouis
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amino Acid Substitution Animals Antibiosis Bacillus thuringiensis - genetics Bacillus thuringiensis - metabolism Codon Cry2Aa Endotoxins - chemistry Endotoxins - genetics Endotoxins - toxicity Gene Expression Hydrophobic and Hydrophilic Interactions Hydrophobicity surface Insect Control Models, Molecular Molecular Sequence Data Moths - microbiology Mutation Protein Conformation Protein Stability Proteolysis Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - toxicity Sequence Alignment Thermostability
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