Glutamine deamidation: an indicator of antiquity, or preservational quality?
Rationale Much credence has been given in the paleoproteomic community to glutamine deamidation as a proxy for the age of proteins derived from fossil and subfossil material, and this modification has been invoked as a means for determining the endogeneity of molecules recovered from very old fossil...
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| Veröffentlicht in: | Rapid communications in mass spectrometry 2016-01, Vol.30 (2), p.251-255 |
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| creator | Schroeter, Elena R. Cleland, Timothy P. |
| description | Rationale
Much credence has been given in the paleoproteomic community to glutamine deamidation as a proxy for the age of proteins derived from fossil and subfossil material, and this modification has been invoked as a means for determining the endogeneity of molecules recovered from very old fossil specimens.
Methods
We re‐evaluated the relationship between glutamine deamidation and geologic time by examining previously published data from five recent mass spectrometry studies of archeaological fossils. Deamidation values recovered for fossils were graphed against their reported chronologic age using WebPlotDigitizer.
Results
The experimental data that has been produced from fossil material to date show that the extent of glutamine deamidation does not correspond to the absolute age of the specimens being examined, but rather show extreme variation between specimens of similar age and taxonomic affinity.
Conclusions
Because deamidation rates and levels can be greatly affected by numerous chemical and environmental factors, we propose that glutamine deamidation is better suited as an indicator of preservational quality and/or environmental conditions than a mark of the endogeneity or authenticity of ancient proteins. Copyright © 2015 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/rcm.7445 |
| format | Article |
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Much credence has been given in the paleoproteomic community to glutamine deamidation as a proxy for the age of proteins derived from fossil and subfossil material, and this modification has been invoked as a means for determining the endogeneity of molecules recovered from very old fossil specimens.
Methods
We re‐evaluated the relationship between glutamine deamidation and geologic time by examining previously published data from five recent mass spectrometry studies of archeaological fossils. Deamidation values recovered for fossils were graphed against their reported chronologic age using WebPlotDigitizer.
Results
The experimental data that has been produced from fossil material to date show that the extent of glutamine deamidation does not correspond to the absolute age of the specimens being examined, but rather show extreme variation between specimens of similar age and taxonomic affinity.
Conclusions
Because deamidation rates and levels can be greatly affected by numerous chemical and environmental factors, we propose that glutamine deamidation is better suited as an indicator of preservational quality and/or environmental conditions than a mark of the endogeneity or authenticity of ancient proteins. Copyright © 2015 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0951-4198</identifier><identifier>EISSN: 1097-0231</identifier><identifier>DOI: 10.1002/rcm.7445</identifier><identifier>PMID: 26689157</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Amides - chemistry ; Fossils ; Glutamine - chemistry ; Proteins - chemistry</subject><ispartof>Rapid communications in mass spectrometry, 2016-01, Vol.30 (2), p.251-255</ispartof><rights>Copyright © 2015 John Wiley & Sons, Ltd.</rights><rights>Copyright © 2016 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4875-3851f6889676741142dd0542895236e12f0816e81eb929a04b7ef33dab6337d53</citedby><cites>FETCH-LOGICAL-c4875-3851f6889676741142dd0542895236e12f0816e81eb929a04b7ef33dab6337d53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Frcm.7445$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Frcm.7445$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26689157$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schroeter, Elena R.</creatorcontrib><creatorcontrib>Cleland, Timothy P.</creatorcontrib><title>Glutamine deamidation: an indicator of antiquity, or preservational quality?</title><title>Rapid communications in mass spectrometry</title><addtitle>Rapid Commun. Mass Spectrom</addtitle><description>Rationale
Much credence has been given in the paleoproteomic community to glutamine deamidation as a proxy for the age of proteins derived from fossil and subfossil material, and this modification has been invoked as a means for determining the endogeneity of molecules recovered from very old fossil specimens.
Methods
We re‐evaluated the relationship between glutamine deamidation and geologic time by examining previously published data from five recent mass spectrometry studies of archeaological fossils. Deamidation values recovered for fossils were graphed against their reported chronologic age using WebPlotDigitizer.
Results
The experimental data that has been produced from fossil material to date show that the extent of glutamine deamidation does not correspond to the absolute age of the specimens being examined, but rather show extreme variation between specimens of similar age and taxonomic affinity.
Conclusions
Because deamidation rates and levels can be greatly affected by numerous chemical and environmental factors, we propose that glutamine deamidation is better suited as an indicator of preservational quality and/or environmental conditions than a mark of the endogeneity or authenticity of ancient proteins. Copyright © 2015 John Wiley & Sons, Ltd.</description><subject>Amides - chemistry</subject><subject>Fossils</subject><subject>Glutamine - chemistry</subject><subject>Proteins - chemistry</subject><issn>0951-4198</issn><issn>1097-0231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10MtqGzEUBmBRWmrnAn2CMtBNFplUR3dlE4LTOgUngZDgpZBnNCB3LrY0k8RvXzl2Uwh0dZD08evwI_QF8BlgTL6HojmTjPEPaAxYyxwTCh_RGGsOOQOtRuggxiXGAJzgz2hEhFAauByj2bQeetv41mWlS7O0ve_a88y2mW9LX9i-C1lXpXPv14PvN6dZulgFF114erW2ztaDrdPTxRH6VNk6uuP9PESPP388TK7z2d301-RylhdMSZ5TxaESSmkhhWQAjJQl5owozQkVDkiFFQinwC000RazhXQVpaVdCEplyekhOtnlrkK3HlzsTeNj4eratq4bogHJIf2kNE302zu67IaQln5VWBBgUv4LLEIXY3CVWQXf2LAxgM22YZMaNtuGE_26DxwWjSvf4N9KE8h34NnXbvPfIHM_udkH7r2PvXt58zb8NkJSyc38dmqu54rw-RU1jP4BPnmRVA</recordid><startdate>20160130</startdate><enddate>20160130</enddate><creator>Schroeter, Elena R.</creator><creator>Cleland, Timothy P.</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>JQ2</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20160130</creationdate><title>Glutamine deamidation: an indicator of antiquity, or preservational quality?</title><author>Schroeter, Elena R. ; Cleland, Timothy P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4875-3851f6889676741142dd0542895236e12f0816e81eb929a04b7ef33dab6337d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Amides - chemistry</topic><topic>Fossils</topic><topic>Glutamine - chemistry</topic><topic>Proteins - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schroeter, Elena R.</creatorcontrib><creatorcontrib>Cleland, Timothy P.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Rapid communications in mass spectrometry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schroeter, Elena R.</au><au>Cleland, Timothy P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glutamine deamidation: an indicator of antiquity, or preservational quality?</atitle><jtitle>Rapid communications in mass spectrometry</jtitle><addtitle>Rapid Commun. Mass Spectrom</addtitle><date>2016-01-30</date><risdate>2016</risdate><volume>30</volume><issue>2</issue><spage>251</spage><epage>255</epage><pages>251-255</pages><issn>0951-4198</issn><eissn>1097-0231</eissn><abstract>Rationale
Much credence has been given in the paleoproteomic community to glutamine deamidation as a proxy for the age of proteins derived from fossil and subfossil material, and this modification has been invoked as a means for determining the endogeneity of molecules recovered from very old fossil specimens.
Methods
We re‐evaluated the relationship between glutamine deamidation and geologic time by examining previously published data from five recent mass spectrometry studies of archeaological fossils. Deamidation values recovered for fossils were graphed against their reported chronologic age using WebPlotDigitizer.
Results
The experimental data that has been produced from fossil material to date show that the extent of glutamine deamidation does not correspond to the absolute age of the specimens being examined, but rather show extreme variation between specimens of similar age and taxonomic affinity.
Conclusions
Because deamidation rates and levels can be greatly affected by numerous chemical and environmental factors, we propose that glutamine deamidation is better suited as an indicator of preservational quality and/or environmental conditions than a mark of the endogeneity or authenticity of ancient proteins. Copyright © 2015 John Wiley & Sons, Ltd.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>26689157</pmid><doi>10.1002/rcm.7445</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Rapid communications in mass spectrometry, 2016-01, Vol.30 (2), p.251-255 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Amides - chemistry Fossils Glutamine - chemistry Proteins - chemistry |
| title | Glutamine deamidation: an indicator of antiquity, or preservational quality? |
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