Functionally active NKG2A-expressing natural killer cells are elevated in rheumatoid arthritis patients compared to psoriatic arthritis patients and healthy donors
Natural killer cell receptors (NKR) have been implicated in rheumatoid (RA) and psoriatic arthritis (PsA) pathogenesis. To gain more insight into their role, we characterised NKR (co-)expression patterns on NK and T cells and NK cell function in RA and PsA. The frequency of NK and T cells expressing...
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| Veröffentlicht in: | Clinical and experimental rheumatology 2015-11, Vol.33 (6), p.795-804 |
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| creator | van Bijnen, S T A Cossu, M Roeven, M W H Jansen, T L Preijers, F Spanholtz, J Dolstra, H Radstake, T R D J |
| description | Natural killer cell receptors (NKR) have been implicated in rheumatoid (RA) and psoriatic arthritis (PsA) pathogenesis. To gain more insight into their role, we characterised NKR (co-)expression patterns on NK and T cells and NK cell function in RA and PsA.
The frequency of NK and T cells expressing killer like immunoglobulin (KIR) and NKG2 receptors and natural cytotoxicity receptors was assessed by 10-colour flow cytometry in peripheral blood of 23 RA, 12 PsA patients and 18 healthy donors (HD). NK cell cytotoxicity and IFN-gamma production was assessed in 8 RA patients and 8 HD.
In RA but not PsA, the frequency of NK cells (median; range) expressing NKG2A (42%; 14-81%) was elevated compared to HD (23%; 9-58%). NKG2A⁺ NK cells predominantly lack KIR, but display normal cytotoxicity and IFN-γ production. In contrast, RA patients with normal NKG2A⁺ NK cell frequency have less functional NK cells compared to HD. T cells expressing Fc-gamma receptor CD16 were elevated in RA (median 0.75%) versus HD (0.3%). Furthermore, T cells expressing the KIRs CD158ah in both RA (0.7%) and PsA (0.3%), and CD158e1e2 in RA (1.5%) were elevated compared to HD (0.2% and 0.4%, respectively). In RA, CD4⁺ T cells expressing the KIRs CD158ah, CD158b1b2j and CD158e1e2 were low ( |
| format | Article |
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The frequency of NK and T cells expressing killer like immunoglobulin (KIR) and NKG2 receptors and natural cytotoxicity receptors was assessed by 10-colour flow cytometry in peripheral blood of 23 RA, 12 PsA patients and 18 healthy donors (HD). NK cell cytotoxicity and IFN-gamma production was assessed in 8 RA patients and 8 HD.
In RA but not PsA, the frequency of NK cells (median; range) expressing NKG2A (42%; 14-81%) was elevated compared to HD (23%; 9-58%). NKG2A⁺ NK cells predominantly lack KIR, but display normal cytotoxicity and IFN-γ production. In contrast, RA patients with normal NKG2A⁺ NK cell frequency have less functional NK cells compared to HD. T cells expressing Fc-gamma receptor CD16 were elevated in RA (median 0.75%) versus HD (0.3%). Furthermore, T cells expressing the KIRs CD158ah in both RA (0.7%) and PsA (0.3%), and CD158e1e2 in RA (1.5%) were elevated compared to HD (0.2% and 0.4%, respectively). In RA, CD4⁺ T cells expressing the KIRs CD158ah, CD158b1b2j and CD158e1e2 were low (<2%) but significantly elevated compared to HD.
This study demonstrates the presence of an elevated, functionally active NKG2A⁺ KIR- NK cell population in RA. Together with an elevated frequency of NKR-expressing T cells, these changes may reflect differential pathogenetic involvement.</description><identifier>ISSN: 0392-856X</identifier><identifier>PMID: 26411696</identifier><language>eng</language><publisher>Italy</publisher><subject>Adult ; Aged ; Arthritis, Psoriatic - immunology ; Arthritis, Rheumatoid - immunology ; Female ; Humans ; Immunity, Cellular ; Killer Cells, Natural - immunology ; Lymphocyte Count ; Male ; Middle Aged ; NK Cell Lectin-Like Receptor Subfamily C - immunology ; Receptors, KIR - immunology ; Receptors, Natural Cytotoxicity Triggering - immunology</subject><ispartof>Clinical and experimental rheumatology, 2015-11, Vol.33 (6), p.795-804</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26411696$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>van Bijnen, S T A</creatorcontrib><creatorcontrib>Cossu, M</creatorcontrib><creatorcontrib>Roeven, M W H</creatorcontrib><creatorcontrib>Jansen, T L</creatorcontrib><creatorcontrib>Preijers, F</creatorcontrib><creatorcontrib>Spanholtz, J</creatorcontrib><creatorcontrib>Dolstra, H</creatorcontrib><creatorcontrib>Radstake, T R D J</creatorcontrib><title>Functionally active NKG2A-expressing natural killer cells are elevated in rheumatoid arthritis patients compared to psoriatic arthritis patients and healthy donors</title><title>Clinical and experimental rheumatology</title><addtitle>Clin Exp Rheumatol</addtitle><description>Natural killer cell receptors (NKR) have been implicated in rheumatoid (RA) and psoriatic arthritis (PsA) pathogenesis. To gain more insight into their role, we characterised NKR (co-)expression patterns on NK and T cells and NK cell function in RA and PsA.
The frequency of NK and T cells expressing killer like immunoglobulin (KIR) and NKG2 receptors and natural cytotoxicity receptors was assessed by 10-colour flow cytometry in peripheral blood of 23 RA, 12 PsA patients and 18 healthy donors (HD). NK cell cytotoxicity and IFN-gamma production was assessed in 8 RA patients and 8 HD.
In RA but not PsA, the frequency of NK cells (median; range) expressing NKG2A (42%; 14-81%) was elevated compared to HD (23%; 9-58%). NKG2A⁺ NK cells predominantly lack KIR, but display normal cytotoxicity and IFN-γ production. In contrast, RA patients with normal NKG2A⁺ NK cell frequency have less functional NK cells compared to HD. T cells expressing Fc-gamma receptor CD16 were elevated in RA (median 0.75%) versus HD (0.3%). Furthermore, T cells expressing the KIRs CD158ah in both RA (0.7%) and PsA (0.3%), and CD158e1e2 in RA (1.5%) were elevated compared to HD (0.2% and 0.4%, respectively). In RA, CD4⁺ T cells expressing the KIRs CD158ah, CD158b1b2j and CD158e1e2 were low (<2%) but significantly elevated compared to HD.
This study demonstrates the presence of an elevated, functionally active NKG2A⁺ KIR- NK cell population in RA. Together with an elevated frequency of NKR-expressing T cells, these changes may reflect differential pathogenetic involvement.</description><subject>Adult</subject><subject>Aged</subject><subject>Arthritis, Psoriatic - immunology</subject><subject>Arthritis, Rheumatoid - immunology</subject><subject>Female</subject><subject>Humans</subject><subject>Immunity, Cellular</subject><subject>Killer Cells, Natural - immunology</subject><subject>Lymphocyte Count</subject><subject>Male</subject><subject>Middle Aged</subject><subject>NK Cell Lectin-Like Receptor Subfamily C - immunology</subject><subject>Receptors, KIR - immunology</subject><subject>Receptors, Natural Cytotoxicity Triggering - immunology</subject><issn>0392-856X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkLFOwzAQhjOAaCm8AvLIEsl2YjsZq4oWRAULSGyRY1-JwbGD7VT0eXhRgigb0530ff9J959kc1zUNK8Yf5ll5zG-YUw54-Ism1FeEsJrPs--1qNTyXgnrT0gOa17QA_3G7rM4XMIEKNxr8jJNAZp0buxFgJSYG1EMgACC3uZQCPjUOhg7GXyRk8odcEkE9EgkwGXIlK-H6aERsmjIfpgJqD-E6XTqANpU3dA2jsf4kV2upM2wuVxLrLn9c3T6jbfPm7uVsttPlBCUi5YWYtd2VIiNAOhKlLLCmNRAFBFOdc1xjWtRE0KVRVspxlrW0IAcMmwUFAssuvfu0PwHyPE1PQm_vwqHfgxNkQwUlaMCj6pV0d1bHvQzRBML8Oh-Su2-AZcrneB</recordid><startdate>20151101</startdate><enddate>20151101</enddate><creator>van Bijnen, S T A</creator><creator>Cossu, M</creator><creator>Roeven, M W H</creator><creator>Jansen, T L</creator><creator>Preijers, F</creator><creator>Spanholtz, J</creator><creator>Dolstra, H</creator><creator>Radstake, T R D J</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20151101</creationdate><title>Functionally active NKG2A-expressing natural killer cells are elevated in rheumatoid arthritis patients compared to psoriatic arthritis patients and healthy donors</title><author>van Bijnen, S T A ; Cossu, M ; Roeven, M W H ; Jansen, T L ; Preijers, F ; Spanholtz, J ; Dolstra, H ; Radstake, T R D J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p211t-75497f4b217d5e7c819a80073ee2c266d9009287913c835fd55bb11ee04507ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Arthritis, Psoriatic - immunology</topic><topic>Arthritis, Rheumatoid - immunology</topic><topic>Female</topic><topic>Humans</topic><topic>Immunity, Cellular</topic><topic>Killer Cells, Natural - immunology</topic><topic>Lymphocyte Count</topic><topic>Male</topic><topic>Middle Aged</topic><topic>NK Cell Lectin-Like Receptor Subfamily C - immunology</topic><topic>Receptors, KIR - immunology</topic><topic>Receptors, Natural Cytotoxicity Triggering - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>van Bijnen, S T A</creatorcontrib><creatorcontrib>Cossu, M</creatorcontrib><creatorcontrib>Roeven, M W H</creatorcontrib><creatorcontrib>Jansen, T L</creatorcontrib><creatorcontrib>Preijers, F</creatorcontrib><creatorcontrib>Spanholtz, J</creatorcontrib><creatorcontrib>Dolstra, H</creatorcontrib><creatorcontrib>Radstake, T R D J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical and experimental rheumatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>van Bijnen, S T A</au><au>Cossu, M</au><au>Roeven, M W H</au><au>Jansen, T L</au><au>Preijers, F</au><au>Spanholtz, J</au><au>Dolstra, H</au><au>Radstake, T R D J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functionally active NKG2A-expressing natural killer cells are elevated in rheumatoid arthritis patients compared to psoriatic arthritis patients and healthy donors</atitle><jtitle>Clinical and experimental rheumatology</jtitle><addtitle>Clin Exp Rheumatol</addtitle><date>2015-11-01</date><risdate>2015</risdate><volume>33</volume><issue>6</issue><spage>795</spage><epage>804</epage><pages>795-804</pages><issn>0392-856X</issn><abstract>Natural killer cell receptors (NKR) have been implicated in rheumatoid (RA) and psoriatic arthritis (PsA) pathogenesis. To gain more insight into their role, we characterised NKR (co-)expression patterns on NK and T cells and NK cell function in RA and PsA.
The frequency of NK and T cells expressing killer like immunoglobulin (KIR) and NKG2 receptors and natural cytotoxicity receptors was assessed by 10-colour flow cytometry in peripheral blood of 23 RA, 12 PsA patients and 18 healthy donors (HD). NK cell cytotoxicity and IFN-gamma production was assessed in 8 RA patients and 8 HD.
In RA but not PsA, the frequency of NK cells (median; range) expressing NKG2A (42%; 14-81%) was elevated compared to HD (23%; 9-58%). NKG2A⁺ NK cells predominantly lack KIR, but display normal cytotoxicity and IFN-γ production. In contrast, RA patients with normal NKG2A⁺ NK cell frequency have less functional NK cells compared to HD. T cells expressing Fc-gamma receptor CD16 were elevated in RA (median 0.75%) versus HD (0.3%). Furthermore, T cells expressing the KIRs CD158ah in both RA (0.7%) and PsA (0.3%), and CD158e1e2 in RA (1.5%) were elevated compared to HD (0.2% and 0.4%, respectively). In RA, CD4⁺ T cells expressing the KIRs CD158ah, CD158b1b2j and CD158e1e2 were low (<2%) but significantly elevated compared to HD.
This study demonstrates the presence of an elevated, functionally active NKG2A⁺ KIR- NK cell population in RA. Together with an elevated frequency of NKR-expressing T cells, these changes may reflect differential pathogenetic involvement.</abstract><cop>Italy</cop><pmid>26411696</pmid><tpages>10</tpages></addata></record> |
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| subjects | Adult Aged Arthritis, Psoriatic - immunology Arthritis, Rheumatoid - immunology Female Humans Immunity, Cellular Killer Cells, Natural - immunology Lymphocyte Count Male Middle Aged NK Cell Lectin-Like Receptor Subfamily C - immunology Receptors, KIR - immunology Receptors, Natural Cytotoxicity Triggering - immunology |
| title | Functionally active NKG2A-expressing natural killer cells are elevated in rheumatoid arthritis patients compared to psoriatic arthritis patients and healthy donors |
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