Development of a Sindbis virus expression system that efficiently expresses green fluorescent protein in midguts of Aedes aegypti following per os infection

A double subgenomic Sindbis (dsSIN) virus, MRE/3′2 J/GFP, was constructed to efficiently express green fluorescent protein (GFP) in the midgut of Aedes aegypti following per os infection. The MRE/3′2 J/GFP RNA genome contained the nonstructural genes and cis‐acting sequences of the dsSIN virus, TE/3...

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