Stabilization of Chromobacterium viscosum Lipase (CVL) Against Ultrasound Inactivation by the Pretreatment with Polyethylene Glycol (PEG)
Although ultrasound has been used to accelerate many enzymatic reactions, the low stability of enzymes in such a system still remains a critical issue, limiting its industrial application. Here, we have reported that polyethylene glycol (PEG) pretreatment stabilized Chromobacterium viscosum lipase (...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2015-12, Vol.177 (8), p.1742-1752 |
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| creator | Talukder, Md . Mahabubur Rahman Shiong, Simon Choo Sze |
| description | Although ultrasound has been used to accelerate many enzymatic reactions, the low stability of enzymes in such a system still remains a critical issue, limiting its industrial application. Here, we have reported that polyethylene glycol (PEG) pretreatment stabilized Chromobacterium viscosum lipase (CVL) in ultrasound-assisted water-isooctane emulsion. PEGs of different molecular weights and concentrations were used to pretreat CVL, and the pretreated lipase activities for olive oil hydrolysis were investigated at different ultrasonic powers. The best result was attained with PEG400 at 100 mg/ml for a lipase concentration of 0.02 mg/ml and an ultrasonic power of 106 W. The half-life time of PEG400-treated lipase at 106 W was 54 min, a 27-fold higher than that attained using untreated lipase. Circular dichroism (CD) spectra suggested that PEG increased the rigidity of CVL structure, which favored the lipase stability against ultrasound inactivation. These results have important implications for the exploitation of ultrasound in biocatalytic process. |
| doi_str_mv | 10.1007/s12010-015-1850-5 |
| format | Article |
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Mahabubur Rahman ; Shiong, Simon Choo Sze</creator><creatorcontrib>Talukder, Md . Mahabubur Rahman ; Shiong, Simon Choo Sze</creatorcontrib><description>Although ultrasound has been used to accelerate many enzymatic reactions, the low stability of enzymes in such a system still remains a critical issue, limiting its industrial application. Here, we have reported that polyethylene glycol (PEG) pretreatment stabilized Chromobacterium viscosum lipase (CVL) in ultrasound-assisted water-isooctane emulsion. PEGs of different molecular weights and concentrations were used to pretreat CVL, and the pretreated lipase activities for olive oil hydrolysis were investigated at different ultrasonic powers. The best result was attained with PEG400 at 100 mg/ml for a lipase concentration of 0.02 mg/ml and an ultrasonic power of 106 W. The half-life time of PEG400-treated lipase at 106 W was 54 min, a 27-fold higher than that attained using untreated lipase. Circular dichroism (CD) spectra suggested that PEG increased the rigidity of CVL structure, which favored the lipase stability against ultrasound inactivation. These results have important implications for the exploitation of ultrasound in biocatalytic process.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/s12010-015-1850-5</identifier><identifier>PMID: 26373941</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>biocatalysis ; Biocatalysts ; Biochemistry ; Biotechnology ; Chemistry ; Chemistry and Materials Science ; Chromobacterium viscosum ; Emulsions ; enzymatic reactions ; Enzyme Stability ; Enzymes ; half life ; hydrolysis ; Inactivation ; industrial applications ; Lipase - chemistry ; Lipase - metabolism ; molecular weight ; Olive oil ; Polyethylene glycol ; Polyethylene Glycols - pharmacology ; Rigidity ; Solvents - chemistry ; Ultrasonic Waves ; ultrasonics</subject><ispartof>Applied biochemistry and biotechnology, 2015-12, Vol.177 (8), p.1742-1752</ispartof><rights>Springer Science+Business Media New York 2015</rights><rights>Applied Biochemistry and Biotechnology is a copyright of Springer, 2015.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c536t-b99d3e231f5f86bb7f8030a926a508ce8d0c93524fb9b25e8e8b491a487416dd3</citedby><cites>FETCH-LOGICAL-c536t-b99d3e231f5f86bb7f8030a926a508ce8d0c93524fb9b25e8e8b491a487416dd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12010-015-1850-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12010-015-1850-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26373941$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Talukder, Md . Mahabubur Rahman</creatorcontrib><creatorcontrib>Shiong, Simon Choo Sze</creatorcontrib><title>Stabilization of Chromobacterium viscosum Lipase (CVL) Against Ultrasound Inactivation by the Pretreatment with Polyethylene Glycol (PEG)</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><addtitle>Appl Biochem Biotechnol</addtitle><description>Although ultrasound has been used to accelerate many enzymatic reactions, the low stability of enzymes in such a system still remains a critical issue, limiting its industrial application. Here, we have reported that polyethylene glycol (PEG) pretreatment stabilized Chromobacterium viscosum lipase (CVL) in ultrasound-assisted water-isooctane emulsion. PEGs of different molecular weights and concentrations were used to pretreat CVL, and the pretreated lipase activities for olive oil hydrolysis were investigated at different ultrasonic powers. The best result was attained with PEG400 at 100 mg/ml for a lipase concentration of 0.02 mg/ml and an ultrasonic power of 106 W. The half-life time of PEG400-treated lipase at 106 W was 54 min, a 27-fold higher than that attained using untreated lipase. Circular dichroism (CD) spectra suggested that PEG increased the rigidity of CVL structure, which favored the lipase stability against ultrasound inactivation. These results have important implications for the exploitation of ultrasound in biocatalytic process.</description><subject>biocatalysis</subject><subject>Biocatalysts</subject><subject>Biochemistry</subject><subject>Biotechnology</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chromobacterium viscosum</subject><subject>Emulsions</subject><subject>enzymatic reactions</subject><subject>Enzyme Stability</subject><subject>Enzymes</subject><subject>half life</subject><subject>hydrolysis</subject><subject>Inactivation</subject><subject>industrial applications</subject><subject>Lipase - chemistry</subject><subject>Lipase - metabolism</subject><subject>molecular weight</subject><subject>Olive oil</subject><subject>Polyethylene glycol</subject><subject>Polyethylene Glycols - pharmacology</subject><subject>Rigidity</subject><subject>Solvents - chemistry</subject><subject>Ultrasonic Waves</subject><subject>ultrasonics</subject><issn>0273-2289</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkcFu1DAURSMEotPCB7ABS91MF4FnO3bsZTUqQ6WRGKkMW8tOnBlXSTzYTlH4A_66GaUgxAKx8pN87n16Oln2BsN7DFB-iJgAhhwwy7FgkLNn2QIzJnMgEj_PFkBKmhMi5Fl2HuM9ACaClS-zM8JpSWWBF9nPu6SNa90PnZzvkW_Q6hB8542ukg1u6NCDi5WP07BxRx0tWq6-bq7Q9V67Pia0a1PQ0Q99jW77KeMe5iIzonSwaBtsClanzvYJfXfpgLa-HW06jK3tLVq3Y-VbtNzerK9eZS8a3Ub7-um9yHYfb76sPuWbz-vb1fUmrxjlKTdS1tQSihvWCG5M2QigoCXhmoGorKihkpSRojHSEGaFFaaQWBeiLDCva3qRLefeY_DfBhuT6qYLbdvq3vohKlwyTDBnXPwHShklsizphF7-hd77IfTTIQoLXkpKgbCJwjNVBR9jsI06BtfpMCoM6qRUzUrVpFSdlKpT5u1T82A6W_9O_HI4AWQG4vTV7234Y_U_Wt_NoUZ7pffBRbW7myAOAAQoB_oI1fi04Q</recordid><startdate>20151201</startdate><enddate>20151201</enddate><creator>Talukder, Md . 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Mahabubur Rahman ; Shiong, Simon Choo Sze</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c536t-b99d3e231f5f86bb7f8030a926a508ce8d0c93524fb9b25e8e8b491a487416dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>biocatalysis</topic><topic>Biocatalysts</topic><topic>Biochemistry</topic><topic>Biotechnology</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chromobacterium viscosum</topic><topic>Emulsions</topic><topic>enzymatic reactions</topic><topic>Enzyme Stability</topic><topic>Enzymes</topic><topic>half life</topic><topic>hydrolysis</topic><topic>Inactivation</topic><topic>industrial applications</topic><topic>Lipase - chemistry</topic><topic>Lipase - metabolism</topic><topic>molecular weight</topic><topic>Olive oil</topic><topic>Polyethylene glycol</topic><topic>Polyethylene Glycols - pharmacology</topic><topic>Rigidity</topic><topic>Solvents - chemistry</topic><topic>Ultrasonic Waves</topic><topic>ultrasonics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Talukder, Md . Mahabubur Rahman</creatorcontrib><creatorcontrib>Shiong, Simon Choo Sze</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><jtitle>Applied biochemistry and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Talukder, Md . Mahabubur Rahman</au><au>Shiong, Simon Choo Sze</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stabilization of Chromobacterium viscosum Lipase (CVL) Against Ultrasound Inactivation by the Pretreatment with Polyethylene Glycol (PEG)</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><stitle>Appl Biochem Biotechnol</stitle><addtitle>Appl Biochem Biotechnol</addtitle><date>2015-12-01</date><risdate>2015</risdate><volume>177</volume><issue>8</issue><spage>1742</spage><epage>1752</epage><pages>1742-1752</pages><issn>0273-2289</issn><eissn>1559-0291</eissn><abstract>Although ultrasound has been used to accelerate many enzymatic reactions, the low stability of enzymes in such a system still remains a critical issue, limiting its industrial application. Here, we have reported that polyethylene glycol (PEG) pretreatment stabilized Chromobacterium viscosum lipase (CVL) in ultrasound-assisted water-isooctane emulsion. PEGs of different molecular weights and concentrations were used to pretreat CVL, and the pretreated lipase activities for olive oil hydrolysis were investigated at different ultrasonic powers. The best result was attained with PEG400 at 100 mg/ml for a lipase concentration of 0.02 mg/ml and an ultrasonic power of 106 W. The half-life time of PEG400-treated lipase at 106 W was 54 min, a 27-fold higher than that attained using untreated lipase. Circular dichroism (CD) spectra suggested that PEG increased the rigidity of CVL structure, which favored the lipase stability against ultrasound inactivation. These results have important implications for the exploitation of ultrasound in biocatalytic process.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>26373941</pmid><doi>10.1007/s12010-015-1850-5</doi><tpages>11</tpages></addata></record> |
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| subjects | biocatalysis Biocatalysts Biochemistry Biotechnology Chemistry Chemistry and Materials Science Chromobacterium viscosum Emulsions enzymatic reactions Enzyme Stability Enzymes half life hydrolysis Inactivation industrial applications Lipase - chemistry Lipase - metabolism molecular weight Olive oil Polyethylene glycol Polyethylene Glycols - pharmacology Rigidity Solvents - chemistry Ultrasonic Waves ultrasonics |
| title | Stabilization of Chromobacterium viscosum Lipase (CVL) Against Ultrasound Inactivation by the Pretreatment with Polyethylene Glycol (PEG) |
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