Changes in 16s RNA Gene Microbial Community Profiling by Concentration of Prokaryotic DNA

Microbial metagenomics are hindered in clinical tissue samples as a result of the large relative amount of human DNA in relation to microbial DNA acting as competitive inhibitors of downstream applications. We evaluated the LOOXSTER® Enrichment Kit to separate eukaryotic and prokaryotic DNA in submu...

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Veröffentlicht in:	Journal of microbiological methods 2015-12, Vol.119, p.239-242
Hauptverfasser:	Glassing, Angela, Dowd, Scot E., Galandiuk, Susan, Davis, Brian, Jorden, Jeffrey R., Chiodini, Rodrick J.
Format:	Artikel
Sprache:	eng
Schlagworte:	16s sequencing Bacteria - classification Bacteria - genetics Bacteria - isolation & purification DNA, Bacterial - genetics Eukaryotic human DNA Humans Ileum - microbiology Metagenomics Microbiome Microbiota Prokaryotic DNA Purification RNA, Ribosomal, 16S - genetics Separation
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