Effect of cellular products of potential probiotic bacteria on the immune response of Labeo rohita and susceptibility to Aeromonas hydrophila infection
In the present study, the immunological efficacy of cellular components from the potential probiotic bacteria Bacillus subtilis VSG1, Pseudomonas aeruginosa VSG2, and Lactobacillus plantarum VSG3 was evaluated in Labeo rohita fingerlings. Fish were immunized intraperitoneally with 0.1 mL phosphate-b...
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| Veröffentlicht in: | Fish & shellfish immunology 2015-10, Vol.46 (2), p.716-722 |
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| creator | Giri, Sib Sankar Sen, Shib Sankar Chi, Cheng Kim, Hyoun Joong Yun, Saekil Park, Se Chang Sukumaran, V. |
| description | In the present study, the immunological efficacy of cellular components from the potential probiotic bacteria Bacillus subtilis VSG1, Pseudomonas aeruginosa VSG2, and Lactobacillus plantarum VSG3 was evaluated in Labeo rohita fingerlings. Fish were immunized intraperitoneally with 0.1 mL phosphate-buffer solution (PBS) containing 0.1 mg of any of the following cellular components: intercellular products (ICPs) of VSG1 (BS-ICPs), heat-killed whole cell products of VSG2 (PA-HKWCPs), or ICPs of VSG3 (LP-ICPs). Fish injected with 0.1 mL PBS served as the control. Various immunological parameters, including the expression of immune-related genes, were measured 14 and 21 days post-immunization. The fish were challenged with Aeromonas hydrophila and mortality was recorded up to 21 days post-infection. The results revealed that administration of cellular components significantly increased the activity of serum lysozyme and the alternative complement pathway, phagocytosis, and respiratory bursts throughout the experimental period. Total serum protein, albumin, and globulin levels were significantly higher in experimental groups than in the control up to 14 days post-immunization, and decreased thereafter. With respect to immune-related genes, IL-1β, COX-2, iNOS, and IL-10 were highly (P |
| doi_str_mv | 10.1016/j.fsi.2015.08.012 |
| format | Article |
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•Subcellular components of probiotic bacteria enhanced immunity in Labeo rohita.•Subcellular components can induce the expression of immune-related genes.•Subcellular components enhanced resistant against Aeromonas hydrophila infection.•Components of Lactobacillus plantarum and Pseudomonas aeruginosa are potential vaccine adjuvants.</description><identifier>ISSN: 1050-4648</identifier><identifier>EISSN: 1095-9947</identifier><identifier>DOI: 10.1016/j.fsi.2015.08.012</identifier><identifier>PMID: 26282681</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Aeromonas hydrophila ; Aeromonas hydrophila - physiology ; Animal Feed - analysis ; Animals ; Bacillus subtilis ; Bacillus subtilis - chemistry ; Cellular components ; Cyprinidae ; Cytokines - genetics ; Cytokines - metabolism ; Diet - veterinary ; Disease protection ; Disease Susceptibility - immunology ; Disease Susceptibility - microbiology ; Disease Susceptibility - veterinary ; Fish Diseases - immunology ; Fish Diseases - microbiology ; Fish Proteins - genetics ; Fish Proteins - metabolism ; Gene expression ; Gene Expression Regulation ; Gram-Negative Bacterial Infections - immunology ; Gram-Negative Bacterial Infections - microbiology ; Gram-Negative Bacterial Infections - veterinary ; Immune response ; Immunity, Innate ; Labeo rohita ; Lactobacillus plantarum ; Lactobacillus plantarum - chemistry ; Probiotic bacteria ; Probiotics - administration & dosage ; Probiotics - pharmacology ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - chemistry</subject><ispartof>Fish & shellfish immunology, 2015-10, Vol.46 (2), p.716-722</ispartof><rights>2015 Elsevier Ltd</rights><rights>Copyright © 2015 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-ba929d28987afec592ebeed0efada798feb6cb69238ba37232106cdb73b470c63</citedby><cites>FETCH-LOGICAL-c386t-ba929d28987afec592ebeed0efada798feb6cb69238ba37232106cdb73b470c63</cites><orcidid>0000-0002-2383-1937</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.fsi.2015.08.012$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26282681$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Giri, Sib Sankar</creatorcontrib><creatorcontrib>Sen, Shib Sankar</creatorcontrib><creatorcontrib>Chi, Cheng</creatorcontrib><creatorcontrib>Kim, Hyoun Joong</creatorcontrib><creatorcontrib>Yun, Saekil</creatorcontrib><creatorcontrib>Park, Se Chang</creatorcontrib><creatorcontrib>Sukumaran, V.</creatorcontrib><title>Effect of cellular products of potential probiotic bacteria on the immune response of Labeo rohita and susceptibility to Aeromonas hydrophila infection</title><title>Fish & shellfish immunology</title><addtitle>Fish Shellfish Immunol</addtitle><description>In the present study, the immunological efficacy of cellular components from the potential probiotic bacteria Bacillus subtilis VSG1, Pseudomonas aeruginosa VSG2, and Lactobacillus plantarum VSG3 was evaluated in Labeo rohita fingerlings. Fish were immunized intraperitoneally with 0.1 mL phosphate-buffer solution (PBS) containing 0.1 mg of any of the following cellular components: intercellular products (ICPs) of VSG1 (BS-ICPs), heat-killed whole cell products of VSG2 (PA-HKWCPs), or ICPs of VSG3 (LP-ICPs). Fish injected with 0.1 mL PBS served as the control. Various immunological parameters, including the expression of immune-related genes, were measured 14 and 21 days post-immunization. The fish were challenged with Aeromonas hydrophila and mortality was recorded up to 21 days post-infection. The results revealed that administration of cellular components significantly increased the activity of serum lysozyme and the alternative complement pathway, phagocytosis, and respiratory bursts throughout the experimental period. Total serum protein, albumin, and globulin levels were significantly higher in experimental groups than in the control up to 14 days post-immunization, and decreased thereafter. With respect to immune-related genes, IL-1β, COX-2, iNOS, and IL-10 were highly (P < 0.05) up-regulated in fish immunized with cellular components, compared to the control. The expression of TNF-α and NF-κB was up-regulated in immunized fish up to 14 days post-immunization. Interestingly, fish immunized with LP-ICPs exhibited a significantly higher post–challenge relative percent survival (83.32%), followed by PA-HKWCPs (66.66%), and BS-ICPs (50%). These results indicate that cellular components of probiotic bacteria can influence immune responses, enhance disease protection, and stimulate immune-related gene expression in rohu. Hence, these cellular components may be useful as adjuvants for vaccines in aquaculture.
•Subcellular components of probiotic bacteria enhanced immunity in Labeo rohita.•Subcellular components can induce the expression of immune-related genes.•Subcellular components enhanced resistant against Aeromonas hydrophila infection.•Components of Lactobacillus plantarum and Pseudomonas aeruginosa are potential vaccine adjuvants.</description><subject>Aeromonas hydrophila</subject><subject>Aeromonas hydrophila - physiology</subject><subject>Animal Feed - analysis</subject><subject>Animals</subject><subject>Bacillus subtilis</subject><subject>Bacillus subtilis - chemistry</subject><subject>Cellular components</subject><subject>Cyprinidae</subject><subject>Cytokines - genetics</subject><subject>Cytokines - metabolism</subject><subject>Diet - veterinary</subject><subject>Disease protection</subject><subject>Disease Susceptibility - immunology</subject><subject>Disease Susceptibility - microbiology</subject><subject>Disease Susceptibility - veterinary</subject><subject>Fish Diseases - immunology</subject><subject>Fish Diseases - microbiology</subject><subject>Fish Proteins - genetics</subject><subject>Fish Proteins - metabolism</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Gram-Negative Bacterial Infections - immunology</subject><subject>Gram-Negative Bacterial Infections - microbiology</subject><subject>Gram-Negative Bacterial Infections - veterinary</subject><subject>Immune response</subject><subject>Immunity, Innate</subject><subject>Labeo rohita</subject><subject>Lactobacillus plantarum</subject><subject>Lactobacillus plantarum - chemistry</subject><subject>Probiotic bacteria</subject><subject>Probiotics - administration & dosage</subject><subject>Probiotics - pharmacology</subject><subject>Pseudomonas aeruginosa</subject><subject>Pseudomonas aeruginosa - chemistry</subject><issn>1050-4648</issn><issn>1095-9947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1q3DAUhU1JaX7aB-imaNmNHUkeyxJdhZC2gYFs2rXQzzVzB1tyJbkwT9LXjc2kXZasrrh853B1TlV9ZLRhlInbYzNkbDhlXUNlQxl_U10xqrpaqV1_sb07Wu_ETl5W1zkfKaWiFfRddckFl1xIdlX9eRgGcIXEgTgYx2U0icwp-sWVvC3nWCAUNOO2tRgLOmKNK5DQkBhIOQDBaVoCkAR5jiHDJtsbC5GkeMBiiAme5CU7mAtaHLGcSInkDlKcYjCZHE4-xfmAoyEYtmswhvfV28GMGT68zJvq59eHH_ff6_3Tt8f7u33tWilKbY3iynOpZG9WZac4WABPYTDe9EoOYIWzQvFWWtP2vOWMCudt39pdT51ob6rPZ9_1e78WyEVPmLckTIC4ZM36jnHacy5fgTKpqFCUryg7oy7FnBMMek44mXTSjOqtOn3Ua3V6q05TqdfqVs2nF_vFTuD_Kf52tQJfzgCsefxGSDo7hODAY1pD0z7if-yfAZ5vrZQ</recordid><startdate>201510</startdate><enddate>201510</enddate><creator>Giri, Sib Sankar</creator><creator>Sen, Shib Sankar</creator><creator>Chi, Cheng</creator><creator>Kim, Hyoun Joong</creator><creator>Yun, Saekil</creator><creator>Park, Se Chang</creator><creator>Sukumaran, V.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>F1W</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>L.G</scope><orcidid>https://orcid.org/0000-0002-2383-1937</orcidid></search><sort><creationdate>201510</creationdate><title>Effect of cellular products of potential probiotic bacteria on the immune response of Labeo rohita and susceptibility to Aeromonas hydrophila infection</title><author>Giri, Sib Sankar ; Sen, Shib Sankar ; Chi, Cheng ; Kim, Hyoun Joong ; Yun, Saekil ; Park, Se Chang ; Sukumaran, V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-ba929d28987afec592ebeed0efada798feb6cb69238ba37232106cdb73b470c63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Aeromonas hydrophila</topic><topic>Aeromonas hydrophila - physiology</topic><topic>Animal Feed - analysis</topic><topic>Animals</topic><topic>Bacillus subtilis</topic><topic>Bacillus subtilis - chemistry</topic><topic>Cellular components</topic><topic>Cyprinidae</topic><topic>Cytokines - genetics</topic><topic>Cytokines - metabolism</topic><topic>Diet - veterinary</topic><topic>Disease protection</topic><topic>Disease Susceptibility - immunology</topic><topic>Disease Susceptibility - microbiology</topic><topic>Disease Susceptibility - veterinary</topic><topic>Fish Diseases - immunology</topic><topic>Fish Diseases - microbiology</topic><topic>Fish Proteins - genetics</topic><topic>Fish Proteins - metabolism</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Gram-Negative Bacterial Infections - immunology</topic><topic>Gram-Negative Bacterial Infections - microbiology</topic><topic>Gram-Negative Bacterial Infections - veterinary</topic><topic>Immune response</topic><topic>Immunity, Innate</topic><topic>Labeo rohita</topic><topic>Lactobacillus plantarum</topic><topic>Lactobacillus plantarum - chemistry</topic><topic>Probiotic bacteria</topic><topic>Probiotics - administration & dosage</topic><topic>Probiotics - pharmacology</topic><topic>Pseudomonas aeruginosa</topic><topic>Pseudomonas aeruginosa - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Giri, Sib Sankar</creatorcontrib><creatorcontrib>Sen, Shib Sankar</creatorcontrib><creatorcontrib>Chi, Cheng</creatorcontrib><creatorcontrib>Kim, Hyoun Joong</creatorcontrib><creatorcontrib>Yun, Saekil</creatorcontrib><creatorcontrib>Park, Se Chang</creatorcontrib><creatorcontrib>Sukumaran, V.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Fish & shellfish immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Giri, Sib Sankar</au><au>Sen, Shib Sankar</au><au>Chi, Cheng</au><au>Kim, Hyoun Joong</au><au>Yun, Saekil</au><au>Park, Se Chang</au><au>Sukumaran, V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of cellular products of potential probiotic bacteria on the immune response of Labeo rohita and susceptibility to Aeromonas hydrophila infection</atitle><jtitle>Fish & shellfish immunology</jtitle><addtitle>Fish Shellfish Immunol</addtitle><date>2015-10</date><risdate>2015</risdate><volume>46</volume><issue>2</issue><spage>716</spage><epage>722</epage><pages>716-722</pages><issn>1050-4648</issn><eissn>1095-9947</eissn><abstract>In the present study, the immunological efficacy of cellular components from the potential probiotic bacteria Bacillus subtilis VSG1, Pseudomonas aeruginosa VSG2, and Lactobacillus plantarum VSG3 was evaluated in Labeo rohita fingerlings. Fish were immunized intraperitoneally with 0.1 mL phosphate-buffer solution (PBS) containing 0.1 mg of any of the following cellular components: intercellular products (ICPs) of VSG1 (BS-ICPs), heat-killed whole cell products of VSG2 (PA-HKWCPs), or ICPs of VSG3 (LP-ICPs). Fish injected with 0.1 mL PBS served as the control. Various immunological parameters, including the expression of immune-related genes, were measured 14 and 21 days post-immunization. The fish were challenged with Aeromonas hydrophila and mortality was recorded up to 21 days post-infection. The results revealed that administration of cellular components significantly increased the activity of serum lysozyme and the alternative complement pathway, phagocytosis, and respiratory bursts throughout the experimental period. Total serum protein, albumin, and globulin levels were significantly higher in experimental groups than in the control up to 14 days post-immunization, and decreased thereafter. With respect to immune-related genes, IL-1β, COX-2, iNOS, and IL-10 were highly (P < 0.05) up-regulated in fish immunized with cellular components, compared to the control. The expression of TNF-α and NF-κB was up-regulated in immunized fish up to 14 days post-immunization. Interestingly, fish immunized with LP-ICPs exhibited a significantly higher post–challenge relative percent survival (83.32%), followed by PA-HKWCPs (66.66%), and BS-ICPs (50%). These results indicate that cellular components of probiotic bacteria can influence immune responses, enhance disease protection, and stimulate immune-related gene expression in rohu. Hence, these cellular components may be useful as adjuvants for vaccines in aquaculture.
•Subcellular components of probiotic bacteria enhanced immunity in Labeo rohita.•Subcellular components can induce the expression of immune-related genes.•Subcellular components enhanced resistant against Aeromonas hydrophila infection.•Components of Lactobacillus plantarum and Pseudomonas aeruginosa are potential vaccine adjuvants.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>26282681</pmid><doi>10.1016/j.fsi.2015.08.012</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-2383-1937</orcidid></addata></record> |
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| ispartof | Fish & shellfish immunology, 2015-10, Vol.46 (2), p.716-722 |
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| subjects | Aeromonas hydrophila Aeromonas hydrophila - physiology Animal Feed - analysis Animals Bacillus subtilis Bacillus subtilis - chemistry Cellular components Cyprinidae Cytokines - genetics Cytokines - metabolism Diet - veterinary Disease protection Disease Susceptibility - immunology Disease Susceptibility - microbiology Disease Susceptibility - veterinary Fish Diseases - immunology Fish Diseases - microbiology Fish Proteins - genetics Fish Proteins - metabolism Gene expression Gene Expression Regulation Gram-Negative Bacterial Infections - immunology Gram-Negative Bacterial Infections - microbiology Gram-Negative Bacterial Infections - veterinary Immune response Immunity, Innate Labeo rohita Lactobacillus plantarum Lactobacillus plantarum - chemistry Probiotic bacteria Probiotics - administration & dosage Probiotics - pharmacology Pseudomonas aeruginosa Pseudomonas aeruginosa - chemistry |
| title | Effect of cellular products of potential probiotic bacteria on the immune response of Labeo rohita and susceptibility to Aeromonas hydrophila infection |
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