The potential role of miRNAs 21 and 199-a in early diagnosis of hepatocellular carcinoma
Hepatocellular carcinoma (HCC) is regarded as one of the most common malignancies and among the leading causes of cancer death among the whole world. The most urgent needs are to find sensitive markers for early diagnosis for HCC. MicroRNAs (miRNAs) are reported as a group of small non-coding RNAs t...
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| Veröffentlicht in: | Gene 2016-01, Vol.575 (1), p.66-70 |
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| creator | Amr, Khalda Said Ezzat, Wafaa M. Elhosary, Yasser A. Hegazy, Abdelfattah E. Fahim, Hoda H. Kamel, Refaat R. |
| description | Hepatocellular carcinoma (HCC) is regarded as one of the most common malignancies and among the leading causes of cancer death among the whole world. The most urgent needs are to find sensitive markers for early diagnosis for HCC. MicroRNAs (miRNAs) are reported as a group of small non-coding RNAs that can function as endogenous RNA interference to regulate expression of the targeted genes. This study was conducted to detect the serum and tissue expression of miR 21 and miR 199-a to be applied as early detectors for HCC.
A total of 40 serum and tissue samples (17 samples from chronic hepatitis and 23 samples from HCC patients) were collected. The levels of the two mature miRNAs (miR-21 and miR-199-a) were detected by real time quantitative reverse-transcriptase PCR (RT-qPCR) in sera and tissues of chronic hepatitis and HCC patients. Besides, miR-21 and miR-199-a levels in relation to clinical and pathological factors were explored.
We found that the expression of serum miR-21 was distinctly increased in HCC compared with chronic hepatitis (P |
| doi_str_mv | 10.1016/j.gene.2015.08.038 |
| format | Article |
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A total of 40 serum and tissue samples (17 samples from chronic hepatitis and 23 samples from HCC patients) were collected. The levels of the two mature miRNAs (miR-21 and miR-199-a) were detected by real time quantitative reverse-transcriptase PCR (RT-qPCR) in sera and tissues of chronic hepatitis and HCC patients. Besides, miR-21 and miR-199-a levels in relation to clinical and pathological factors were explored.
We found that the expression of serum miR-21 was distinctly increased in HCC compared with chronic hepatitis (P<0.001). miR 199-a was distinctly decreased in HCC compared with chronic hepatitis (P<0.001). In addition, median of miR 21 was increased in malignant when compared to adjacent non-malignant tissues without significant differences (P=0.191) while miR 199-a was significantly decreased in malignant when compared to adjacent nonmalignant tissues (P<0.001). ROC analysis showed that miR-21 and miR-199-a might be potential biomarkers for HCC.
In conclusion, the expression of miR-21 was significantly up-regulated and miR-199-a was significantly down regulated in serum of patients with HCC. Due to their reasonable sensitivity and specificity for disease progression, miR-21 and miR-199-a could be used as potential circulating biomarkers for HCC.
•We investigated the expression of miRNAs21 199-a in sera of HCC patients•the expression of miR-21 was significantly up-regulated in HCC patients•miR-199-a was significantly down regulated in serum of patients with HCC•miR-21 and miR-199-a could be used as circulating biomarkers for HCC.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/j.gene.2015.08.038</identifier><identifier>PMID: 26302751</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adult ; Biomarkers, Tumor - biosynthesis ; Carcinoma, Hepatocellular - diagnosis ; Carcinoma, Hepatocellular - metabolism ; Carcinoma, Hepatocellular - pathology ; Female ; Gene Expression Regulation, Neoplastic ; HCC –mir 21-mir 199-a ; Hepatitis C, Chronic - diagnosis ; Hepatitis C, Chronic - metabolism ; Hepatitis C, Chronic - pathology ; Humans ; Liver Neoplasms - diagnosis ; Liver Neoplasms - metabolism ; Liver Neoplasms - pathology ; Male ; MicroRNAs - biosynthesis ; Middle Aged ; RNA, Neoplasm - biosynthesis</subject><ispartof>Gene, 2016-01, Vol.575 (1), p.66-70</ispartof><rights>2015 Elsevier B.V.</rights><rights>Copyright © 2015 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c525t-e24688ccffc8a26c252a305508e8d471f6b8da0e8f17fda63474d1788b4a9dd53</citedby><cites>FETCH-LOGICAL-c525t-e24688ccffc8a26c252a305508e8d471f6b8da0e8f17fda63474d1788b4a9dd53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.gene.2015.08.038$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26302751$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Amr, Khalda Said</creatorcontrib><creatorcontrib>Ezzat, Wafaa M.</creatorcontrib><creatorcontrib>Elhosary, Yasser A.</creatorcontrib><creatorcontrib>Hegazy, Abdelfattah E.</creatorcontrib><creatorcontrib>Fahim, Hoda H.</creatorcontrib><creatorcontrib>Kamel, Refaat R.</creatorcontrib><title>The potential role of miRNAs 21 and 199-a in early diagnosis of hepatocellular carcinoma</title><title>Gene</title><addtitle>Gene</addtitle><description>Hepatocellular carcinoma (HCC) is regarded as one of the most common malignancies and among the leading causes of cancer death among the whole world. The most urgent needs are to find sensitive markers for early diagnosis for HCC. MicroRNAs (miRNAs) are reported as a group of small non-coding RNAs that can function as endogenous RNA interference to regulate expression of the targeted genes. This study was conducted to detect the serum and tissue expression of miR 21 and miR 199-a to be applied as early detectors for HCC.
A total of 40 serum and tissue samples (17 samples from chronic hepatitis and 23 samples from HCC patients) were collected. The levels of the two mature miRNAs (miR-21 and miR-199-a) were detected by real time quantitative reverse-transcriptase PCR (RT-qPCR) in sera and tissues of chronic hepatitis and HCC patients. Besides, miR-21 and miR-199-a levels in relation to clinical and pathological factors were explored.
We found that the expression of serum miR-21 was distinctly increased in HCC compared with chronic hepatitis (P<0.001). miR 199-a was distinctly decreased in HCC compared with chronic hepatitis (P<0.001). In addition, median of miR 21 was increased in malignant when compared to adjacent non-malignant tissues without significant differences (P=0.191) while miR 199-a was significantly decreased in malignant when compared to adjacent nonmalignant tissues (P<0.001). ROC analysis showed that miR-21 and miR-199-a might be potential biomarkers for HCC.
In conclusion, the expression of miR-21 was significantly up-regulated and miR-199-a was significantly down regulated in serum of patients with HCC. Due to their reasonable sensitivity and specificity for disease progression, miR-21 and miR-199-a could be used as potential circulating biomarkers for HCC.
•We investigated the expression of miRNAs21 199-a in sera of HCC patients•the expression of miR-21 was significantly up-regulated in HCC patients•miR-199-a was significantly down regulated in serum of patients with HCC•miR-21 and miR-199-a could be used as circulating biomarkers for HCC.</description><subject>Adult</subject><subject>Biomarkers, Tumor - biosynthesis</subject><subject>Carcinoma, Hepatocellular - diagnosis</subject><subject>Carcinoma, Hepatocellular - metabolism</subject><subject>Carcinoma, Hepatocellular - pathology</subject><subject>Female</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>HCC –mir 21-mir 199-a</subject><subject>Hepatitis C, Chronic - diagnosis</subject><subject>Hepatitis C, Chronic - metabolism</subject><subject>Hepatitis C, Chronic - pathology</subject><subject>Humans</subject><subject>Liver Neoplasms - diagnosis</subject><subject>Liver Neoplasms - metabolism</subject><subject>Liver Neoplasms - pathology</subject><subject>Male</subject><subject>MicroRNAs - biosynthesis</subject><subject>Middle Aged</subject><subject>RNA, Neoplasm - biosynthesis</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1LxDAQhoMoun78AQ-So5fWJG3aFLyI-AWiIArewmwy1SxtsyZdwX9vyq4exbnMHJ55mXkIOeYs54xXZ4v8DQfMBeMyZypnhdoiM67qJmNp3iYzVtQq45w3e2Q_xgVLJaXYJXuiKpioJZ-R1-d3pEs_4jA66GjwHVLf0t49PVxEKjiFwVLeNBlQN1CE0H1R6-Bt8NHFiXzHJYzeYNetOgjUQDBu8D0ckp0WuohHm35AXq6vni9vs_vHm7vLi_vMSCHHDEVZKWVM2xoFojJCCijSlUyhsmXN22quLDBULa9bC1VR1qXltVLzEhprZXFATte5y-A_VhhH3bs4nQMD-lXUPL0pmCyl-gdayEKoqiwTKtaoCT7GgK1eBtdD-NKc6Um-XuhJvp7ka6Z0Ep6WTjb5q3mP9nflx3YCztcAJiGfDoOOxuFg0LqAZtTWu7_yvwHM8ZOu</recordid><startdate>20160101</startdate><enddate>20160101</enddate><creator>Amr, Khalda Said</creator><creator>Ezzat, Wafaa M.</creator><creator>Elhosary, Yasser A.</creator><creator>Hegazy, Abdelfattah E.</creator><creator>Fahim, Hoda H.</creator><creator>Kamel, Refaat R.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20160101</creationdate><title>The potential role of miRNAs 21 and 199-a in early diagnosis of hepatocellular carcinoma</title><author>Amr, Khalda Said ; Ezzat, Wafaa M. ; Elhosary, Yasser A. ; Hegazy, Abdelfattah E. ; Fahim, Hoda H. ; Kamel, Refaat R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c525t-e24688ccffc8a26c252a305508e8d471f6b8da0e8f17fda63474d1788b4a9dd53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adult</topic><topic>Biomarkers, Tumor - biosynthesis</topic><topic>Carcinoma, Hepatocellular - diagnosis</topic><topic>Carcinoma, Hepatocellular - metabolism</topic><topic>Carcinoma, Hepatocellular - pathology</topic><topic>Female</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>HCC –mir 21-mir 199-a</topic><topic>Hepatitis C, Chronic - diagnosis</topic><topic>Hepatitis C, Chronic - metabolism</topic><topic>Hepatitis C, Chronic - pathology</topic><topic>Humans</topic><topic>Liver Neoplasms - diagnosis</topic><topic>Liver Neoplasms - metabolism</topic><topic>Liver Neoplasms - pathology</topic><topic>Male</topic><topic>MicroRNAs - biosynthesis</topic><topic>Middle Aged</topic><topic>RNA, Neoplasm - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Amr, Khalda Said</creatorcontrib><creatorcontrib>Ezzat, Wafaa M.</creatorcontrib><creatorcontrib>Elhosary, Yasser A.</creatorcontrib><creatorcontrib>Hegazy, Abdelfattah E.</creatorcontrib><creatorcontrib>Fahim, Hoda H.</creatorcontrib><creatorcontrib>Kamel, Refaat R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Amr, Khalda Said</au><au>Ezzat, Wafaa M.</au><au>Elhosary, Yasser A.</au><au>Hegazy, Abdelfattah E.</au><au>Fahim, Hoda H.</au><au>Kamel, Refaat R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The potential role of miRNAs 21 and 199-a in early diagnosis of hepatocellular carcinoma</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>2016-01-01</date><risdate>2016</risdate><volume>575</volume><issue>1</issue><spage>66</spage><epage>70</epage><pages>66-70</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>Hepatocellular carcinoma (HCC) is regarded as one of the most common malignancies and among the leading causes of cancer death among the whole world. The most urgent needs are to find sensitive markers for early diagnosis for HCC. MicroRNAs (miRNAs) are reported as a group of small non-coding RNAs that can function as endogenous RNA interference to regulate expression of the targeted genes. This study was conducted to detect the serum and tissue expression of miR 21 and miR 199-a to be applied as early detectors for HCC.
A total of 40 serum and tissue samples (17 samples from chronic hepatitis and 23 samples from HCC patients) were collected. The levels of the two mature miRNAs (miR-21 and miR-199-a) were detected by real time quantitative reverse-transcriptase PCR (RT-qPCR) in sera and tissues of chronic hepatitis and HCC patients. Besides, miR-21 and miR-199-a levels in relation to clinical and pathological factors were explored.
We found that the expression of serum miR-21 was distinctly increased in HCC compared with chronic hepatitis (P<0.001). miR 199-a was distinctly decreased in HCC compared with chronic hepatitis (P<0.001). In addition, median of miR 21 was increased in malignant when compared to adjacent non-malignant tissues without significant differences (P=0.191) while miR 199-a was significantly decreased in malignant when compared to adjacent nonmalignant tissues (P<0.001). ROC analysis showed that miR-21 and miR-199-a might be potential biomarkers for HCC.
In conclusion, the expression of miR-21 was significantly up-regulated and miR-199-a was significantly down regulated in serum of patients with HCC. Due to their reasonable sensitivity and specificity for disease progression, miR-21 and miR-199-a could be used as potential circulating biomarkers for HCC.
•We investigated the expression of miRNAs21 199-a in sera of HCC patients•the expression of miR-21 was significantly up-regulated in HCC patients•miR-199-a was significantly down regulated in serum of patients with HCC•miR-21 and miR-199-a could be used as circulating biomarkers for HCC.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>26302751</pmid><doi>10.1016/j.gene.2015.08.038</doi><tpages>5</tpages></addata></record> |
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| subjects | Adult Biomarkers, Tumor - biosynthesis Carcinoma, Hepatocellular - diagnosis Carcinoma, Hepatocellular - metabolism Carcinoma, Hepatocellular - pathology Female Gene Expression Regulation, Neoplastic HCC –mir 21-mir 199-a Hepatitis C, Chronic - diagnosis Hepatitis C, Chronic - metabolism Hepatitis C, Chronic - pathology Humans Liver Neoplasms - diagnosis Liver Neoplasms - metabolism Liver Neoplasms - pathology Male MicroRNAs - biosynthesis Middle Aged RNA, Neoplasm - biosynthesis |
| title | The potential role of miRNAs 21 and 199-a in early diagnosis of hepatocellular carcinoma |
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