Regenerated bacterial cellulose microfluidic column for glycoproteins separation
•A simple strategy to prepare regenerated bacterial cellulose column with concanavalin A lectin immobilized in microfluidic system was applied.•This research represents a significant step to prepare bacterial cellulose as column packing material in microfluidic system.•The microfluidic system presen...
Gespeichert in:
| Veröffentlicht in: | Carbohydrate polymers 2016-02, Vol.137, p.271-276 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 276 |
|---|---|
| container_issue | |
| container_start_page | 271 |
| container_title | Carbohydrate polymers |
| container_volume | 137 |
| creator | Chen, Chuntao Zhu, Chunlin Huang, Yang Nie, Ying Yang, Jiazhi Shen, Ruiqi Sun, Dongping |
| description | •A simple strategy to prepare regenerated bacterial cellulose column with concanavalin A lectin immobilized in microfluidic system was applied.•This research represents a significant step to prepare bacterial cellulose as column packing material in microfluidic system.•The microfluidic system presents great potential application in affinity chromatography of glycoproteins analysis.
To analysis and separate glycoproteins, a simple strategy to prepare regenerated bacterial cellulose (RBC) column with concanavalin A (Con A) lectin immobilized in microfluidic system was applied. RBC was filled into microchannel to fabricate RBC microcolumn after bacterial cellulose dissolved in NaOH–sulfourea water solution. Lectin Con A was covalently connected onto RBC matrix surface via Schiff-base formation. Lysozyme (non-glycoprotein) and transferrin (glycoprotein) were successfully separated based on their different affinities toward the immobilized Con A. Overall, the RBC microfluidic system presents great potential application in affinity chromatography of glycoproteins analysis, and this research represents a significant step to prepare bacterial cellulose (BC) as column packing material in microfluidic system. What is more, troublesome operations for lectin affinity chromatography were simplified by integrating the microfluidic chip onto a HPLC (High Performance Liquid Chromatography) system. |
| doi_str_mv | 10.1016/j.carbpol.2015.10.081 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1751194794</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0144861715010619</els_id><sourcerecordid>1751194794</sourcerecordid><originalsourceid>FETCH-LOGICAL-c468t-efb63e7ee6ad8bb41a7df437e8fd0f6766128a9423b632c1cc027acf980880733</originalsourceid><addsrcrecordid>eNqFkMtKxDAUhoMoOl4eQenSTcecNpOkKxHxBgOK6Dqk6YlkSJsxaQXf3gwzujWbwM93bh8h50DnQIFfreZGx3Yd_LyisMjZnErYIzOQoimhZmyfzCgwVkoO4ogcp7Si-XGgh-So4jzHNZ2Rl1f8wAGjHrErWm1GjE77wqD3kw8Ji96ZGKyfXOdMYYKf-qGwIRYf_tuEdQwjuiEVCdc693BhOCUHVvuEZ7v_hLzf373dPpbL54en25tlaRiXY4m25TUKRK472bYMtOgsqwVK21HLBedQSd2wqs5cZcAYWgltbCOplFTU9Qm53PbNO3xOmEbVu7RZWw8YpqRALAAaJhqW0cUWzZekFNGqdXS9jt8KqNrIVCu1k6k2MjdxlpnrLnYjprbH7q_q114GrrcA5kO_HEaVjMPBYOcimlF1wf0z4gcRions</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1751194794</pqid></control><display><type>article</type><title>Regenerated bacterial cellulose microfluidic column for glycoproteins separation</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Chen, Chuntao ; Zhu, Chunlin ; Huang, Yang ; Nie, Ying ; Yang, Jiazhi ; Shen, Ruiqi ; Sun, Dongping</creator><creatorcontrib>Chen, Chuntao ; Zhu, Chunlin ; Huang, Yang ; Nie, Ying ; Yang, Jiazhi ; Shen, Ruiqi ; Sun, Dongping</creatorcontrib><description>•A simple strategy to prepare regenerated bacterial cellulose column with concanavalin A lectin immobilized in microfluidic system was applied.•This research represents a significant step to prepare bacterial cellulose as column packing material in microfluidic system.•The microfluidic system presents great potential application in affinity chromatography of glycoproteins analysis.
To analysis and separate glycoproteins, a simple strategy to prepare regenerated bacterial cellulose (RBC) column with concanavalin A (Con A) lectin immobilized in microfluidic system was applied. RBC was filled into microchannel to fabricate RBC microcolumn after bacterial cellulose dissolved in NaOH–sulfourea water solution. Lectin Con A was covalently connected onto RBC matrix surface via Schiff-base formation. Lysozyme (non-glycoprotein) and transferrin (glycoprotein) were successfully separated based on their different affinities toward the immobilized Con A. Overall, the RBC microfluidic system presents great potential application in affinity chromatography of glycoproteins analysis, and this research represents a significant step to prepare bacterial cellulose (BC) as column packing material in microfluidic system. What is more, troublesome operations for lectin affinity chromatography were simplified by integrating the microfluidic chip onto a HPLC (High Performance Liquid Chromatography) system.</description><identifier>ISSN: 0144-8617</identifier><identifier>EISSN: 1879-1344</identifier><identifier>DOI: 10.1016/j.carbpol.2015.10.081</identifier><identifier>PMID: 26686130</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Cellulose - chemistry ; Chromatography, High Pressure Liquid ; Column packing ; Concanavalin A - chemistry ; Glycoproteins - chemistry ; Glycoproteins separation ; Microfluidic ; Microfluidics - methods ; Muramidase - chemistry ; Regenerated bacterial cellulose ; Transferrin - chemistry</subject><ispartof>Carbohydrate polymers, 2016-02, Vol.137, p.271-276</ispartof><rights>2015 Elsevier Ltd</rights><rights>Copyright © 2015 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c468t-efb63e7ee6ad8bb41a7df437e8fd0f6766128a9423b632c1cc027acf980880733</citedby><cites>FETCH-LOGICAL-c468t-efb63e7ee6ad8bb41a7df437e8fd0f6766128a9423b632c1cc027acf980880733</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.carbpol.2015.10.081$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26686130$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Chuntao</creatorcontrib><creatorcontrib>Zhu, Chunlin</creatorcontrib><creatorcontrib>Huang, Yang</creatorcontrib><creatorcontrib>Nie, Ying</creatorcontrib><creatorcontrib>Yang, Jiazhi</creatorcontrib><creatorcontrib>Shen, Ruiqi</creatorcontrib><creatorcontrib>Sun, Dongping</creatorcontrib><title>Regenerated bacterial cellulose microfluidic column for glycoproteins separation</title><title>Carbohydrate polymers</title><addtitle>Carbohydr Polym</addtitle><description>•A simple strategy to prepare regenerated bacterial cellulose column with concanavalin A lectin immobilized in microfluidic system was applied.•This research represents a significant step to prepare bacterial cellulose as column packing material in microfluidic system.•The microfluidic system presents great potential application in affinity chromatography of glycoproteins analysis.
To analysis and separate glycoproteins, a simple strategy to prepare regenerated bacterial cellulose (RBC) column with concanavalin A (Con A) lectin immobilized in microfluidic system was applied. RBC was filled into microchannel to fabricate RBC microcolumn after bacterial cellulose dissolved in NaOH–sulfourea water solution. Lectin Con A was covalently connected onto RBC matrix surface via Schiff-base formation. Lysozyme (non-glycoprotein) and transferrin (glycoprotein) were successfully separated based on their different affinities toward the immobilized Con A. Overall, the RBC microfluidic system presents great potential application in affinity chromatography of glycoproteins analysis, and this research represents a significant step to prepare bacterial cellulose (BC) as column packing material in microfluidic system. What is more, troublesome operations for lectin affinity chromatography were simplified by integrating the microfluidic chip onto a HPLC (High Performance Liquid Chromatography) system.</description><subject>Cellulose - chemistry</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Column packing</subject><subject>Concanavalin A - chemistry</subject><subject>Glycoproteins - chemistry</subject><subject>Glycoproteins separation</subject><subject>Microfluidic</subject><subject>Microfluidics - methods</subject><subject>Muramidase - chemistry</subject><subject>Regenerated bacterial cellulose</subject><subject>Transferrin - chemistry</subject><issn>0144-8617</issn><issn>1879-1344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKxDAUhoMoOl4eQenSTcecNpOkKxHxBgOK6Dqk6YlkSJsxaQXf3gwzujWbwM93bh8h50DnQIFfreZGx3Yd_LyisMjZnErYIzOQoimhZmyfzCgwVkoO4ogcp7Si-XGgh-So4jzHNZ2Rl1f8wAGjHrErWm1GjE77wqD3kw8Ji96ZGKyfXOdMYYKf-qGwIRYf_tuEdQwjuiEVCdc693BhOCUHVvuEZ7v_hLzf373dPpbL54en25tlaRiXY4m25TUKRK472bYMtOgsqwVK21HLBedQSd2wqs5cZcAYWgltbCOplFTU9Qm53PbNO3xOmEbVu7RZWw8YpqRALAAaJhqW0cUWzZekFNGqdXS9jt8KqNrIVCu1k6k2MjdxlpnrLnYjprbH7q_q114GrrcA5kO_HEaVjMPBYOcimlF1wf0z4gcRions</recordid><startdate>20160210</startdate><enddate>20160210</enddate><creator>Chen, Chuntao</creator><creator>Zhu, Chunlin</creator><creator>Huang, Yang</creator><creator>Nie, Ying</creator><creator>Yang, Jiazhi</creator><creator>Shen, Ruiqi</creator><creator>Sun, Dongping</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20160210</creationdate><title>Regenerated bacterial cellulose microfluidic column for glycoproteins separation</title><author>Chen, Chuntao ; Zhu, Chunlin ; Huang, Yang ; Nie, Ying ; Yang, Jiazhi ; Shen, Ruiqi ; Sun, Dongping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c468t-efb63e7ee6ad8bb41a7df437e8fd0f6766128a9423b632c1cc027acf980880733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Cellulose - chemistry</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Column packing</topic><topic>Concanavalin A - chemistry</topic><topic>Glycoproteins - chemistry</topic><topic>Glycoproteins separation</topic><topic>Microfluidic</topic><topic>Microfluidics - methods</topic><topic>Muramidase - chemistry</topic><topic>Regenerated bacterial cellulose</topic><topic>Transferrin - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Chuntao</creatorcontrib><creatorcontrib>Zhu, Chunlin</creatorcontrib><creatorcontrib>Huang, Yang</creatorcontrib><creatorcontrib>Nie, Ying</creatorcontrib><creatorcontrib>Yang, Jiazhi</creatorcontrib><creatorcontrib>Shen, Ruiqi</creatorcontrib><creatorcontrib>Sun, Dongping</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Carbohydrate polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Chuntao</au><au>Zhu, Chunlin</au><au>Huang, Yang</au><au>Nie, Ying</au><au>Yang, Jiazhi</au><au>Shen, Ruiqi</au><au>Sun, Dongping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regenerated bacterial cellulose microfluidic column for glycoproteins separation</atitle><jtitle>Carbohydrate polymers</jtitle><addtitle>Carbohydr Polym</addtitle><date>2016-02-10</date><risdate>2016</risdate><volume>137</volume><spage>271</spage><epage>276</epage><pages>271-276</pages><issn>0144-8617</issn><eissn>1879-1344</eissn><abstract>•A simple strategy to prepare regenerated bacterial cellulose column with concanavalin A lectin immobilized in microfluidic system was applied.•This research represents a significant step to prepare bacterial cellulose as column packing material in microfluidic system.•The microfluidic system presents great potential application in affinity chromatography of glycoproteins analysis.
To analysis and separate glycoproteins, a simple strategy to prepare regenerated bacterial cellulose (RBC) column with concanavalin A (Con A) lectin immobilized in microfluidic system was applied. RBC was filled into microchannel to fabricate RBC microcolumn after bacterial cellulose dissolved in NaOH–sulfourea water solution. Lectin Con A was covalently connected onto RBC matrix surface via Schiff-base formation. Lysozyme (non-glycoprotein) and transferrin (glycoprotein) were successfully separated based on their different affinities toward the immobilized Con A. Overall, the RBC microfluidic system presents great potential application in affinity chromatography of glycoproteins analysis, and this research represents a significant step to prepare bacterial cellulose (BC) as column packing material in microfluidic system. What is more, troublesome operations for lectin affinity chromatography were simplified by integrating the microfluidic chip onto a HPLC (High Performance Liquid Chromatography) system.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>26686130</pmid><doi>10.1016/j.carbpol.2015.10.081</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0144-8617 |
| ispartof | Carbohydrate polymers, 2016-02, Vol.137, p.271-276 |
| issn | 0144-8617 1879-1344 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1751194794 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Cellulose - chemistry Chromatography, High Pressure Liquid Column packing Concanavalin A - chemistry Glycoproteins - chemistry Glycoproteins separation Microfluidic Microfluidics - methods Muramidase - chemistry Regenerated bacterial cellulose Transferrin - chemistry |
| title | Regenerated bacterial cellulose microfluidic column for glycoproteins separation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-14T12%3A34%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Regenerated%20bacterial%20cellulose%20microfluidic%20column%20for%20glycoproteins%20separation&rft.jtitle=Carbohydrate%20polymers&rft.au=Chen,%20Chuntao&rft.date=2016-02-10&rft.volume=137&rft.spage=271&rft.epage=276&rft.pages=271-276&rft.issn=0144-8617&rft.eissn=1879-1344&rft_id=info:doi/10.1016/j.carbpol.2015.10.081&rft_dat=%3Cproquest_cross%3E1751194794%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1751194794&rft_id=info:pmid/26686130&rft_els_id=S0144861715010619&rfr_iscdi=true |