Ultrastructural changes induced in HeLa cells after phototoxic treatment with harmine
In the present work, we have continued our studies on harmine phototoxicity in human tumour cells. The toxicity of harmine in the dark was analysed by a quantitative neutral red uptake assay, and subcellular sensitive targets following harmine photosensitization were defined by electron microscopic a...
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| Veröffentlicht in: | Journal of applied toxicology 2004-05, Vol.24 (3), p.197-201 |
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| creator | Pérez Martín, J. M. Labrador, V. Fernández Freire, P. Molero, M. L. Hazen, M. J. |
| description | In the present work, we have continued our studies on harmine phototoxicity in human tumour cells. The toxicity of harmine in the dark was analysed by a quantitative neutral red uptake assay, and subcellular sensitive targets following harmine photosensitization were defined by electron microscopic analysis of HeLa cells. The results obtained indicated that this compound shows a clear dose‐dependent cytotoxic effect in the dark. The combined treatment with suitable doses of harmine and UV radiation was very effective at an early stage, although maximal cell killing appeared 48 h after photodynamic activation. Ultrastructural examination of HeLa cells immediately after the photodynamic treatment revealed lysosomal destabilization and profound cytoplasmic vacuolization that evolved to cytolysis, which is typical of necrotic cell death. It is concluded that harmine could be a valuable photosensitizer whose biological applications merit further evaluation. Copyright © 2004 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/jat.972 |
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M. ; Labrador, V. ; Fernández Freire, P. ; Molero, M. L. ; Hazen, M. J.</creator><creatorcontrib>Pérez Martín, J. M. ; Labrador, V. ; Fernández Freire, P. ; Molero, M. L. ; Hazen, M. J.</creatorcontrib><description>In the present work, we have continued our studies on harmine phototoxicity in human tumour cells. The toxicity of harmine in the dark was analysed by a quantitative neutral red uptake assay, and subcellular sensitive targets following harmine photosensitization were defined by electron microscopic analysis of HeLa cells. The results obtained indicated that this compound shows a clear dose‐dependent cytotoxic effect in the dark. The combined treatment with suitable doses of harmine and UV radiation was very effective at an early stage, although maximal cell killing appeared 48 h after photodynamic activation. Ultrastructural examination of HeLa cells immediately after the photodynamic treatment revealed lysosomal destabilization and profound cytoplasmic vacuolization that evolved to cytolysis, which is typical of necrotic cell death. It is concluded that harmine could be a valuable photosensitizer whose biological applications merit further evaluation. Copyright © 2004 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0260-437X</identifier><identifier>EISSN: 1099-1263</identifier><identifier>DOI: 10.1002/jat.972</identifier><identifier>PMID: 15211613</identifier><identifier>CODEN: JJATDK</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Biological and medical sciences ; cell death ; Cell Survival ; Hallucinogens - toxicity ; harmine ; Harmine - toxicity ; HeLa cells ; HeLa Cells - ultrastructure ; Humans ; lysosomes ; Medical sciences ; Microscopy, Electron, Transmission ; Photoradiation therapy and photosensitizing agent ; Photosensitivity Disorders - chemically induced ; photosensitization ; TEM ; Treatment with physical agents ; Treatment. 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M.</creatorcontrib><creatorcontrib>Labrador, V.</creatorcontrib><creatorcontrib>Fernández Freire, P.</creatorcontrib><creatorcontrib>Molero, M. L.</creatorcontrib><creatorcontrib>Hazen, M. J.</creatorcontrib><title>Ultrastructural changes induced in HeLa cells after phototoxic treatment with harmine</title><title>Journal of applied toxicology</title><addtitle>J. Appl. Toxicol</addtitle><description>In the present work, we have continued our studies on harmine phototoxicity in human tumour cells. The toxicity of harmine in the dark was analysed by a quantitative neutral red uptake assay, and subcellular sensitive targets following harmine photosensitization were defined by electron microscopic analysis of HeLa cells. The results obtained indicated that this compound shows a clear dose‐dependent cytotoxic effect in the dark. The combined treatment with suitable doses of harmine and UV radiation was very effective at an early stage, although maximal cell killing appeared 48 h after photodynamic activation. Ultrastructural examination of HeLa cells immediately after the photodynamic treatment revealed lysosomal destabilization and profound cytoplasmic vacuolization that evolved to cytolysis, which is typical of necrotic cell death. It is concluded that harmine could be a valuable photosensitizer whose biological applications merit further evaluation. Copyright © 2004 John Wiley & Sons, Ltd.</description><subject>Biological and medical sciences</subject><subject>cell death</subject><subject>Cell Survival</subject><subject>Hallucinogens - toxicity</subject><subject>harmine</subject><subject>Harmine - toxicity</subject><subject>HeLa cells</subject><subject>HeLa Cells - ultrastructure</subject><subject>Humans</subject><subject>lysosomes</subject><subject>Medical sciences</subject><subject>Microscopy, Electron, Transmission</subject><subject>Photoradiation therapy and photosensitizing agent</subject><subject>Photosensitivity Disorders - chemically induced</subject><subject>photosensitization</subject><subject>TEM</subject><subject>Treatment with physical agents</subject><subject>Treatment. 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J.</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>200405</creationdate><title>Ultrastructural changes induced in HeLa cells after phototoxic treatment with harmine</title><author>Pérez Martín, J. M. ; Labrador, V. ; Fernández Freire, P. ; Molero, M. L. ; Hazen, M. 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| subjects | Biological and medical sciences cell death Cell Survival Hallucinogens - toxicity harmine Harmine - toxicity HeLa cells HeLa Cells - ultrastructure Humans lysosomes Medical sciences Microscopy, Electron, Transmission Photoradiation therapy and photosensitizing agent Photosensitivity Disorders - chemically induced photosensitization TEM Treatment with physical agents Treatment. General aspects Tumors |
| title | Ultrastructural changes induced in HeLa cells after phototoxic treatment with harmine |
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