Improved method for transport of living cell cultures
An easy and cost-effective method for transport of living cell cultures which avoids the use of dry ice and prevents bacterial contamination is described. Cells are suspended in buffered culture medium in sealed and insulated 2 ml cryovials and are able to grow and survive in substantial numbers dur...
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Veröffentlicht in: | Biotechnology letters 2000-03, Vol.22 (5), p.383-385 |
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container_title | Biotechnology letters |
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creator | RAINOV, N. G TRÜMPLER, C QUINONES, A SPEAR, M. A KRAMM, C. M |
description | An easy and cost-effective method for transport of living cell cultures which avoids the use of dry ice and prevents bacterial contamination is described. Cells are suspended in buffered culture medium in sealed and insulated 2 ml cryovials and are able to grow and survive in substantial numbers during several days of storage and shipment at ambient temperature. Replating results in an identical repopulation in all cell lines. Not only tumor cells but also fibroblasts seem to tolerate well this improved method for shipment.[PUBLICATION ABSTRACT] |
doi_str_mv | 10.1023/A:1005624718463 |
format | Article |
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subjects | Ambient temperature Animal cells Bacteria Biological and medical sciences Biotechnology Cell culture Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Miscellaneous Repopulation |
title | Improved method for transport of living cell cultures |
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