Improved method for transport of living cell cultures

An easy and cost-effective method for transport of living cell cultures which avoids the use of dry ice and prevents bacterial contamination is described. Cells are suspended in buffered culture medium in sealed and insulated 2 ml cryovials and are able to grow and survive in substantial numbers dur...

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Veröffentlicht in:Biotechnology letters 2000-03, Vol.22 (5), p.383-385
Hauptverfasser: RAINOV, N. G, TRÜMPLER, C, QUINONES, A, SPEAR, M. A, KRAMM, C. M
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container_end_page 385
container_issue 5
container_start_page 383
container_title Biotechnology letters
container_volume 22
creator RAINOV, N. G
TRÜMPLER, C
QUINONES, A
SPEAR, M. A
KRAMM, C. M
description An easy and cost-effective method for transport of living cell cultures which avoids the use of dry ice and prevents bacterial contamination is described. Cells are suspended in buffered culture medium in sealed and insulated 2 ml cryovials and are able to grow and survive in substantial numbers during several days of storage and shipment at ambient temperature. Replating results in an identical repopulation in all cell lines. Not only tumor cells but also fibroblasts seem to tolerate well this improved method for shipment.[PUBLICATION ABSTRACT]
doi_str_mv 10.1023/A:1005624718463
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ispartof Biotechnology letters, 2000-03, Vol.22 (5), p.383-385
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subjects Ambient temperature
Animal cells
Bacteria
Biological and medical sciences
Biotechnology
Cell culture
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Miscellaneous
Repopulation
title Improved method for transport of living cell cultures
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