Enhanced Expression of miR-425 Promotes Esophageal Squamous Cell Carcinoma Tumorigenesis by Targeting SMAD2
Esophageal squamous cell carcinoma （ESCC） is one of the most common and deadly cancers in the world, Currently, clinical therapy of ESCC remains limited and the five-year survival rate is poor. The function of miR-425 has been reported in multiple human cancers. However. the tumorigenic role and cli...
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| Veröffentlicht in: | Journal of genetics and genomics 2015-11, Vol.42 (11), p.601-611 |
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| creator | Liu, Lingyan Zhao, Zitong Zhou, Wei Fan, Xinyi Zhan, Qimin Song, Yongmei |
| description | Esophageal squamous cell carcinoma （ESCC） is one of the most common and deadly cancers in the world, Currently, clinical therapy of ESCC remains limited and the five-year survival rate is poor. The function of miR-425 has been reported in multiple human cancers. However. the tumorigenic role and clinical significance of miR-425 in ESCC remains unclear. We found that enhanced expression of miR- 425 in ESCC cell lines not only promoted cell proliferation and colony formation, but also increased cellular metastasis. Furthermore, we revealed the mechanism that miR-425 inhibited the expression of SMAD2 by targeting the second binding site in the 3＇-untranslated region （3＇-UTR） in ESCC. This mode of action influenced not only SMAD2 rnRNA expression but also protein expression. In addition, we detected the expression of miR-425 in ESCC tissues and plasma. Moreover, we analyzed the relationship between miR-425 expression and SMAD2 mRNA expression. We found that miR-425 was overexpressed in ESCC tissues and the plasma relative to adjacent normal tissues and plasma of healthy individuals. Furthermore, there was a negative correlation between miR-425 expression and SMAD2, Taken together, our results show that miR-425 functions as an oncogene by targeting the 3＇-UTR of SMAD2 and indicate the potential utility of plasma miR-425 as a novel biomarker for ESCC diagnosis. |
| doi_str_mv | 10.1016/j.jgg.2015.09.010 |
| format | Article |
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The function of miR-425 has been reported in multiple human cancers. However. the tumorigenic role and clinical significance of miR-425 in ESCC remains unclear. We found that enhanced expression of miR- 425 in ESCC cell lines not only promoted cell proliferation and colony formation, but also increased cellular metastasis. Furthermore, we revealed the mechanism that miR-425 inhibited the expression of SMAD2 by targeting the second binding site in the 3＇-untranslated region （3＇-UTR） in ESCC. This mode of action influenced not only SMAD2 rnRNA expression but also protein expression. In addition, we detected the expression of miR-425 in ESCC tissues and plasma. Moreover, we analyzed the relationship between miR-425 expression and SMAD2 mRNA expression. We found that miR-425 was overexpressed in ESCC tissues and the plasma relative to adjacent normal tissues and plasma of healthy individuals. Furthermore, there was a negative correlation between miR-425 expression and SMAD2, Taken together, our results show that miR-425 functions as an oncogene by targeting the 3＇-UTR of SMAD2 and indicate the potential utility of plasma miR-425 as a novel biomarker for ESCC diagnosis.</description><identifier>ISSN: 1673-8527</identifier><identifier>DOI: 10.1016/j.jgg.2015.09.010</identifier><identifier>PMID: 26674378</identifier><language>eng</language><publisher>China: Elsevier Ltd</publisher><subject>3' Untranslated Regions ; Carcinogenesis ; Carcinoma, Squamous Cell - genetics ; Carcinoma, Squamous Cell - metabolism ; Carcinoma, Squamous Cell - pathology ; Carcinoma, Squamous Cell - physiopathology ; Cell Line, Tumor ; Cell Proliferation ; Esophageal Neoplasms - genetics ; Esophageal Neoplasms - metabolism ; Esophageal Neoplasms - pathology ; Esophageal Neoplasms - physiopathology ; Esophageal Squamous Cell Carcinoma ; Gene Expression Regulation, Neoplastic ; Humans ; MicroRNAs - genetics ; MicroRNAs - metabolism ; miR-425 ; mRNA表达 ; Plasma miRNA ; SMAD2 ; Smad2 Protein - genetics ; Smad2 Protein - metabolism ; Up-Regulation ; 临床意义 ; 临床治疗 ; 生物标志物 ; 癌组织 ; 食管癌 ; 鳞状细胞癌</subject><ispartof>Journal of genetics and genomics, 2015-11, Vol.42 (11), p.601-611</ispartof><rights>2015 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China</rights><rights>Copyright © 2015 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c380t-9e5240db38976e6700c04be4d10926e9ac208b53ca70b7965a7cab55359209573</citedby><cites>FETCH-LOGICAL-c380t-9e5240db38976e6700c04be4d10926e9ac208b53ca70b7965a7cab55359209573</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/95085X/95085X.jpg</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jgg.2015.09.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26674378$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Lingyan</creatorcontrib><creatorcontrib>Zhao, Zitong</creatorcontrib><creatorcontrib>Zhou, Wei</creatorcontrib><creatorcontrib>Fan, Xinyi</creatorcontrib><creatorcontrib>Zhan, Qimin</creatorcontrib><creatorcontrib>Song, Yongmei</creatorcontrib><title>Enhanced Expression of miR-425 Promotes Esophageal Squamous Cell Carcinoma Tumorigenesis by Targeting SMAD2</title><title>Journal of genetics and genomics</title><addtitle>Journal of Genetics and Genomics</addtitle><description>Esophageal squamous cell carcinoma （ESCC） is one of the most common and deadly cancers in the world, Currently, clinical therapy of ESCC remains limited and the five-year survival rate is poor. The function of miR-425 has been reported in multiple human cancers. However. the tumorigenic role and clinical significance of miR-425 in ESCC remains unclear. We found that enhanced expression of miR- 425 in ESCC cell lines not only promoted cell proliferation and colony formation, but also increased cellular metastasis. Furthermore, we revealed the mechanism that miR-425 inhibited the expression of SMAD2 by targeting the second binding site in the 3＇-untranslated region （3＇-UTR） in ESCC. This mode of action influenced not only SMAD2 rnRNA expression but also protein expression. In addition, we detected the expression of miR-425 in ESCC tissues and plasma. Moreover, we analyzed the relationship between miR-425 expression and SMAD2 mRNA expression. We found that miR-425 was overexpressed in ESCC tissues and the plasma relative to adjacent normal tissues and plasma of healthy individuals. Furthermore, there was a negative correlation between miR-425 expression and SMAD2, Taken together, our results show that miR-425 functions as an oncogene by targeting the 3＇-UTR of SMAD2 and indicate the potential utility of plasma miR-425 as a novel biomarker for ESCC diagnosis.</description><subject>3' Untranslated Regions</subject><subject>Carcinogenesis</subject><subject>Carcinoma, Squamous Cell - genetics</subject><subject>Carcinoma, Squamous Cell - metabolism</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Carcinoma, Squamous Cell - physiopathology</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation</subject><subject>Esophageal Neoplasms - genetics</subject><subject>Esophageal Neoplasms - metabolism</subject><subject>Esophageal Neoplasms - pathology</subject><subject>Esophageal Neoplasms - physiopathology</subject><subject>Esophageal Squamous Cell Carcinoma</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>miR-425</subject><subject>mRNA表达</subject><subject>Plasma miRNA</subject><subject>SMAD2</subject><subject>Smad2 Protein - genetics</subject><subject>Smad2 Protein - metabolism</subject><subject>Up-Regulation</subject><subject>临床意义</subject><subject>临床治疗</subject><subject>生物标志物</subject><subject>癌组织</subject><subject>食管癌</subject><subject>鳞状细胞癌</subject><issn>1673-8527</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1v0zAYx3MAsTH4AFyQxYlLwmM7tmNxmkp5kYY2sXK2HOdp6tLErZ2g7dvjqt2OnJ7L_-35FcU7ChUFKj9tq23fVwyoqEBXQOFFcUml4mUjmLooXqe0BRCNpuJVccGkVDVXzWXxZzlu7OiwI8uHfcSUfBhJWJPB_yprJshdDEOYMJFlCvuN7dHuyP1htkOYE1ngbkcWNjo_hsGS1TyE6HscMflE2keysrHHyY89uf95_YW9KV6u7S7h2_O9Kn5_Xa4W38ub228_Ftc3peMNTKVGwWroWt5oJVEqAAd1i3VHQTOJ2joGTSu4swpapaWwytlWCC40Ay0Uvyo-nnL3MRxmTJMZfHJ5qx0xzzZUCaiZ5LLOUnqSuhhSirg2--gHGx8NBXPkarYmczVHrga0yVyz5_05fm4H7J4dT1Cz4PNJgPnJvx6jSc7jEbKP6CbTBf_f-A_nSZsw9odM77khFwCtG835PyY0lKo</recordid><startdate>20151120</startdate><enddate>20151120</enddate><creator>Liu, Lingyan</creator><creator>Zhao, Zitong</creator><creator>Zhou, Wei</creator><creator>Fan, Xinyi</creator><creator>Zhan, Qimin</creator><creator>Song, Yongmei</creator><general>Elsevier Ltd</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20151120</creationdate><title>Enhanced Expression of miR-425 Promotes Esophageal Squamous Cell Carcinoma Tumorigenesis by Targeting SMAD2</title><author>Liu, Lingyan ; Zhao, Zitong ; Zhou, Wei ; Fan, Xinyi ; Zhan, Qimin ; Song, Yongmei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c380t-9e5240db38976e6700c04be4d10926e9ac208b53ca70b7965a7cab55359209573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>3' Untranslated Regions</topic><topic>Carcinogenesis</topic><topic>Carcinoma, Squamous Cell - genetics</topic><topic>Carcinoma, Squamous Cell - metabolism</topic><topic>Carcinoma, Squamous Cell - pathology</topic><topic>Carcinoma, Squamous Cell - physiopathology</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation</topic><topic>Esophageal Neoplasms - genetics</topic><topic>Esophageal Neoplasms - metabolism</topic><topic>Esophageal Neoplasms - pathology</topic><topic>Esophageal Neoplasms - physiopathology</topic><topic>Esophageal Squamous Cell Carcinoma</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>miR-425</topic><topic>mRNA表达</topic><topic>Plasma miRNA</topic><topic>SMAD2</topic><topic>Smad2 Protein - genetics</topic><topic>Smad2 Protein - metabolism</topic><topic>Up-Regulation</topic><topic>临床意义</topic><topic>临床治疗</topic><topic>生物标志物</topic><topic>癌组织</topic><topic>食管癌</topic><topic>鳞状细胞癌</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Lingyan</creatorcontrib><creatorcontrib>Zhao, Zitong</creatorcontrib><creatorcontrib>Zhou, Wei</creatorcontrib><creatorcontrib>Fan, Xinyi</creatorcontrib><creatorcontrib>Zhan, Qimin</creatorcontrib><creatorcontrib>Song, Yongmei</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库-自然科学-生物科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of genetics and genomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Lingyan</au><au>Zhao, Zitong</au><au>Zhou, Wei</au><au>Fan, Xinyi</au><au>Zhan, Qimin</au><au>Song, Yongmei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhanced Expression of miR-425 Promotes Esophageal Squamous Cell Carcinoma Tumorigenesis by Targeting SMAD2</atitle><jtitle>Journal of genetics and genomics</jtitle><addtitle>Journal of Genetics and Genomics</addtitle><date>2015-11-20</date><risdate>2015</risdate><volume>42</volume><issue>11</issue><spage>601</spage><epage>611</epage><pages>601-611</pages><issn>1673-8527</issn><abstract>Esophageal squamous cell carcinoma （ESCC） is one of the most common and deadly cancers in the world, Currently, clinical therapy of ESCC remains limited and the five-year survival rate is poor. The function of miR-425 has been reported in multiple human cancers. However. the tumorigenic role and clinical significance of miR-425 in ESCC remains unclear. We found that enhanced expression of miR- 425 in ESCC cell lines not only promoted cell proliferation and colony formation, but also increased cellular metastasis. Furthermore, we revealed the mechanism that miR-425 inhibited the expression of SMAD2 by targeting the second binding site in the 3＇-untranslated region （3＇-UTR） in ESCC. This mode of action influenced not only SMAD2 rnRNA expression but also protein expression. In addition, we detected the expression of miR-425 in ESCC tissues and plasma. Moreover, we analyzed the relationship between miR-425 expression and SMAD2 mRNA expression. We found that miR-425 was overexpressed in ESCC tissues and the plasma relative to adjacent normal tissues and plasma of healthy individuals. Furthermore, there was a negative correlation between miR-425 expression and SMAD2, Taken together, our results show that miR-425 functions as an oncogene by targeting the 3＇-UTR of SMAD2 and indicate the potential utility of plasma miR-425 as a novel biomarker for ESCC diagnosis.</abstract><cop>China</cop><pub>Elsevier Ltd</pub><pmid>26674378</pmid><doi>10.1016/j.jgg.2015.09.010</doi><tpages>11</tpages></addata></record> |
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| subjects | 3' Untranslated Regions Carcinogenesis Carcinoma, Squamous Cell - genetics Carcinoma, Squamous Cell - metabolism Carcinoma, Squamous Cell - pathology Carcinoma, Squamous Cell - physiopathology Cell Line, Tumor Cell Proliferation Esophageal Neoplasms - genetics Esophageal Neoplasms - metabolism Esophageal Neoplasms - pathology Esophageal Neoplasms - physiopathology Esophageal Squamous Cell Carcinoma Gene Expression Regulation, Neoplastic Humans MicroRNAs - genetics MicroRNAs - metabolism miR-425 mRNA表达 Plasma miRNA SMAD2 Smad2 Protein - genetics Smad2 Protein - metabolism Up-Regulation 临床意义 临床治疗 生物标志物 癌组织 食管癌 鳞状细胞癌 |
| title | Enhanced Expression of miR-425 Promotes Esophageal Squamous Cell Carcinoma Tumorigenesis by Targeting SMAD2 |
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