Over-expression of Gastrodia anti-fungal protein enhances Verticillium wilt resistance in coloured cotton
Currently there are no adequate control measures for the cotton fungal diseases Verticillium wilt and Fusarium wilt, which are important factors limiting yield under certain conditions. In this study the gene encoding a Gastrodia anti-fungal protein was introduced into three cultivars of coloured co...
Gespeichert in:
| Veröffentlicht in: | Plant breeding 2004-10, Vol.123 (5), p.454-459 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 459 |
|---|---|
| container_issue | 5 |
| container_start_page | 454 |
| container_title | Plant breeding |
| container_volume | 123 |
| creator | Wang, Y.Q Chen, D.J Wang, D.M Huang, Q.S Yao, Z.P Liu, F.J Wei, X.W Li, R.J Zhang, Z.N Sun, Y.R |
| description | Currently there are no adequate control measures for the cotton fungal diseases Verticillium wilt and Fusarium wilt, which are important factors limiting yield under certain conditions. In this study the gene encoding a Gastrodia anti-fungal protein was introduced into three cultivars of coloured cotton using the method of pollen-tube pathway transformation, with the purpose of obtaining transgenic plants with improved resistance to wilt. Of the 121 herbicide-resistant cotton plants two, LB-5-8 and ZB-1-49, were scored as transgenic based on Southern blot, RT-PCR analysis and in vitro anti-fungal activity assay. Field analysis demonstrated that the transgenic lines LB-5-8 and ZB-1-49 possess an increased resistance to wilt. After 2 years of breeding, the progeny of LB-5-8 and ZB-1-49 lines still showed a stable and strong resistance to Verticillium wilt. Lines with high levels of resistance to Verticillium wilt obtained from the present study may be widely planted and help to reduce the future impact of cotton wilt on cotton production resulting in increased yields. |
| doi_str_mv | 10.1111/j.1439-0523.2004.01005.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_17504094</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17504094</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4334-b586aa1ef711276eb3d5e34123e85855d7ecd8c72916c835310b10f9788adc023</originalsourceid><addsrcrecordid>eNqNkMFu1DAQhiMEEkvhGfAFbkln4jh2JC5QlQVU0YpSys3yOpPiJRsvdkK3b49DqnLFF9ua7x-PvyxjCAWmdbwtsOJNDqLkRQlQFYAAojg8ylYPhcfZClA2eSMqfJo9i3EL853LVebOf1PI6bAPFKPzA_MdW5s4Bt86w8wwurybhhvTs33wI7mB0fDDDJYi-0ZhdNb1vZt27Nb1I0s9XBznKkug9b2fArXpMI5-eJ496Uwf6cX9fpRdvT_9evIhPztffzx5e5bbivMq3whVG4PUScRS1rThrSBeYclJCSVEK8m2ysqywdoqLjjCBqFrpFKmtVDyo-z10jcN_GuiOOqdi5b63gzkp6hRCqigqRKoFtAGH2OgTu-D25lwpxH07FZv9axQzwr17Fb_dasPKfrq_g0Trem7kP7s4r98jQpBYOLeLFzSQ3f_3V9fvPtSiXnCfIknq3R4iJvwU9eSS6GvP6_1RV1ff6_FJ32Z-JcL3xmvzU1II11dloAcoBFKyZL_Adq3pfU</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17504094</pqid></control><display><type>article</type><title>Over-expression of Gastrodia anti-fungal protein enhances Verticillium wilt resistance in coloured cotton</title><source>Wiley Online Library Journals Frontfile Complete</source><creator>Wang, Y.Q ; Chen, D.J ; Wang, D.M ; Huang, Q.S ; Yao, Z.P ; Liu, F.J ; Wei, X.W ; Li, R.J ; Zhang, Z.N ; Sun, Y.R</creator><creatorcontrib>Wang, Y.Q ; Chen, D.J ; Wang, D.M ; Huang, Q.S ; Yao, Z.P ; Liu, F.J ; Wei, X.W ; Li, R.J ; Zhang, Z.N ; Sun, Y.R</creatorcontrib><description>Currently there are no adequate control measures for the cotton fungal diseases Verticillium wilt and Fusarium wilt, which are important factors limiting yield under certain conditions. In this study the gene encoding a Gastrodia anti-fungal protein was introduced into three cultivars of coloured cotton using the method of pollen-tube pathway transformation, with the purpose of obtaining transgenic plants with improved resistance to wilt. Of the 121 herbicide-resistant cotton plants two, LB-5-8 and ZB-1-49, were scored as transgenic based on Southern blot, RT-PCR analysis and in vitro anti-fungal activity assay. Field analysis demonstrated that the transgenic lines LB-5-8 and ZB-1-49 possess an increased resistance to wilt. After 2 years of breeding, the progeny of LB-5-8 and ZB-1-49 lines still showed a stable and strong resistance to Verticillium wilt. Lines with high levels of resistance to Verticillium wilt obtained from the present study may be widely planted and help to reduce the future impact of cotton wilt on cotton production resulting in increased yields.</description><identifier>ISSN: 0179-9541</identifier><identifier>EISSN: 1439-0523</identifier><identifier>DOI: 10.1111/j.1439-0523.2004.01005.x</identifier><identifier>CODEN: PLABED</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Agronomy. Soil science and plant productions ; antifungal properties ; antifungal proteins ; Biological and medical sciences ; colored cotton cultivars ; cotton ; cultivars ; disease resistance ; Fundamental and applied biological sciences. Psychology ; Fusarium ; Gastrodia ; Gastrodia anti-fungal protein ; Gastrodia elata ; gene overexpression ; Genetic engineering applications ; genetic resistance ; Genetics and breeding of economic plants ; Gossypium hirsutum ; Plant breeding: fundamental aspects and methodology ; plant pathogenic fungi ; pollen-tube pathway transformation ; transgenes ; transgenic coloured cotton ; transgenic plants ; Varietal selection. Specialized plant breeding, plant breeding aims ; Verticillium ; Verticillium dahliae ; Verticillium wilt</subject><ispartof>Plant breeding, 2004-10, Vol.123 (5), p.454-459</ispartof><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4334-b586aa1ef711276eb3d5e34123e85855d7ecd8c72916c835310b10f9788adc023</citedby><cites>FETCH-LOGICAL-c4334-b586aa1ef711276eb3d5e34123e85855d7ecd8c72916c835310b10f9788adc023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1439-0523.2004.01005.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1439-0523.2004.01005.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16181051$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Y.Q</creatorcontrib><creatorcontrib>Chen, D.J</creatorcontrib><creatorcontrib>Wang, D.M</creatorcontrib><creatorcontrib>Huang, Q.S</creatorcontrib><creatorcontrib>Yao, Z.P</creatorcontrib><creatorcontrib>Liu, F.J</creatorcontrib><creatorcontrib>Wei, X.W</creatorcontrib><creatorcontrib>Li, R.J</creatorcontrib><creatorcontrib>Zhang, Z.N</creatorcontrib><creatorcontrib>Sun, Y.R</creatorcontrib><title>Over-expression of Gastrodia anti-fungal protein enhances Verticillium wilt resistance in coloured cotton</title><title>Plant breeding</title><description>Currently there are no adequate control measures for the cotton fungal diseases Verticillium wilt and Fusarium wilt, which are important factors limiting yield under certain conditions. In this study the gene encoding a Gastrodia anti-fungal protein was introduced into three cultivars of coloured cotton using the method of pollen-tube pathway transformation, with the purpose of obtaining transgenic plants with improved resistance to wilt. Of the 121 herbicide-resistant cotton plants two, LB-5-8 and ZB-1-49, were scored as transgenic based on Southern blot, RT-PCR analysis and in vitro anti-fungal activity assay. Field analysis demonstrated that the transgenic lines LB-5-8 and ZB-1-49 possess an increased resistance to wilt. After 2 years of breeding, the progeny of LB-5-8 and ZB-1-49 lines still showed a stable and strong resistance to Verticillium wilt. Lines with high levels of resistance to Verticillium wilt obtained from the present study may be widely planted and help to reduce the future impact of cotton wilt on cotton production resulting in increased yields.</description><subject>Agronomy. Soil science and plant productions</subject><subject>antifungal properties</subject><subject>antifungal proteins</subject><subject>Biological and medical sciences</subject><subject>colored cotton cultivars</subject><subject>cotton</subject><subject>cultivars</subject><subject>disease resistance</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fusarium</subject><subject>Gastrodia</subject><subject>Gastrodia anti-fungal protein</subject><subject>Gastrodia elata</subject><subject>gene overexpression</subject><subject>Genetic engineering applications</subject><subject>genetic resistance</subject><subject>Genetics and breeding of economic plants</subject><subject>Gossypium hirsutum</subject><subject>Plant breeding: fundamental aspects and methodology</subject><subject>plant pathogenic fungi</subject><subject>pollen-tube pathway transformation</subject><subject>transgenes</subject><subject>transgenic coloured cotton</subject><subject>transgenic plants</subject><subject>Varietal selection. Specialized plant breeding, plant breeding aims</subject><subject>Verticillium</subject><subject>Verticillium dahliae</subject><subject>Verticillium wilt</subject><issn>0179-9541</issn><issn>1439-0523</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNqNkMFu1DAQhiMEEkvhGfAFbkln4jh2JC5QlQVU0YpSys3yOpPiJRsvdkK3b49DqnLFF9ua7x-PvyxjCAWmdbwtsOJNDqLkRQlQFYAAojg8ylYPhcfZClA2eSMqfJo9i3EL853LVebOf1PI6bAPFKPzA_MdW5s4Bt86w8wwurybhhvTs33wI7mB0fDDDJYi-0ZhdNb1vZt27Nb1I0s9XBznKkug9b2fArXpMI5-eJ496Uwf6cX9fpRdvT_9evIhPztffzx5e5bbivMq3whVG4PUScRS1rThrSBeYclJCSVEK8m2ysqywdoqLjjCBqFrpFKmtVDyo-z10jcN_GuiOOqdi5b63gzkp6hRCqigqRKoFtAGH2OgTu-D25lwpxH07FZv9axQzwr17Fb_dasPKfrq_g0Trem7kP7s4r98jQpBYOLeLFzSQ3f_3V9fvPtSiXnCfIknq3R4iJvwU9eSS6GvP6_1RV1ff6_FJ32Z-JcL3xmvzU1II11dloAcoBFKyZL_Adq3pfU</recordid><startdate>200410</startdate><enddate>200410</enddate><creator>Wang, Y.Q</creator><creator>Chen, D.J</creator><creator>Wang, D.M</creator><creator>Huang, Q.S</creator><creator>Yao, Z.P</creator><creator>Liu, F.J</creator><creator>Wei, X.W</creator><creator>Li, R.J</creator><creator>Zhang, Z.N</creator><creator>Sun, Y.R</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>200410</creationdate><title>Over-expression of Gastrodia anti-fungal protein enhances Verticillium wilt resistance in coloured cotton</title><author>Wang, Y.Q ; Chen, D.J ; Wang, D.M ; Huang, Q.S ; Yao, Z.P ; Liu, F.J ; Wei, X.W ; Li, R.J ; Zhang, Z.N ; Sun, Y.R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4334-b586aa1ef711276eb3d5e34123e85855d7ecd8c72916c835310b10f9788adc023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>antifungal properties</topic><topic>antifungal proteins</topic><topic>Biological and medical sciences</topic><topic>colored cotton cultivars</topic><topic>cotton</topic><topic>cultivars</topic><topic>disease resistance</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fusarium</topic><topic>Gastrodia</topic><topic>Gastrodia anti-fungal protein</topic><topic>Gastrodia elata</topic><topic>gene overexpression</topic><topic>Genetic engineering applications</topic><topic>genetic resistance</topic><topic>Genetics and breeding of economic plants</topic><topic>Gossypium hirsutum</topic><topic>Plant breeding: fundamental aspects and methodology</topic><topic>plant pathogenic fungi</topic><topic>pollen-tube pathway transformation</topic><topic>transgenes</topic><topic>transgenic coloured cotton</topic><topic>transgenic plants</topic><topic>Varietal selection. Specialized plant breeding, plant breeding aims</topic><topic>Verticillium</topic><topic>Verticillium dahliae</topic><topic>Verticillium wilt</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Y.Q</creatorcontrib><creatorcontrib>Chen, D.J</creatorcontrib><creatorcontrib>Wang, D.M</creatorcontrib><creatorcontrib>Huang, Q.S</creatorcontrib><creatorcontrib>Yao, Z.P</creatorcontrib><creatorcontrib>Liu, F.J</creatorcontrib><creatorcontrib>Wei, X.W</creatorcontrib><creatorcontrib>Li, R.J</creatorcontrib><creatorcontrib>Zhang, Z.N</creatorcontrib><creatorcontrib>Sun, Y.R</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Plant breeding</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Y.Q</au><au>Chen, D.J</au><au>Wang, D.M</au><au>Huang, Q.S</au><au>Yao, Z.P</au><au>Liu, F.J</au><au>Wei, X.W</au><au>Li, R.J</au><au>Zhang, Z.N</au><au>Sun, Y.R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Over-expression of Gastrodia anti-fungal protein enhances Verticillium wilt resistance in coloured cotton</atitle><jtitle>Plant breeding</jtitle><date>2004-10</date><risdate>2004</risdate><volume>123</volume><issue>5</issue><spage>454</spage><epage>459</epage><pages>454-459</pages><issn>0179-9541</issn><eissn>1439-0523</eissn><coden>PLABED</coden><abstract>Currently there are no adequate control measures for the cotton fungal diseases Verticillium wilt and Fusarium wilt, which are important factors limiting yield under certain conditions. In this study the gene encoding a Gastrodia anti-fungal protein was introduced into three cultivars of coloured cotton using the method of pollen-tube pathway transformation, with the purpose of obtaining transgenic plants with improved resistance to wilt. Of the 121 herbicide-resistant cotton plants two, LB-5-8 and ZB-1-49, were scored as transgenic based on Southern blot, RT-PCR analysis and in vitro anti-fungal activity assay. Field analysis demonstrated that the transgenic lines LB-5-8 and ZB-1-49 possess an increased resistance to wilt. After 2 years of breeding, the progeny of LB-5-8 and ZB-1-49 lines still showed a stable and strong resistance to Verticillium wilt. Lines with high levels of resistance to Verticillium wilt obtained from the present study may be widely planted and help to reduce the future impact of cotton wilt on cotton production resulting in increased yields.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/j.1439-0523.2004.01005.x</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0179-9541 |
| ispartof | Plant breeding, 2004-10, Vol.123 (5), p.454-459 |
| issn | 0179-9541 1439-0523 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17504094 |
| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Agronomy. Soil science and plant productions antifungal properties antifungal proteins Biological and medical sciences colored cotton cultivars cotton cultivars disease resistance Fundamental and applied biological sciences. Psychology Fusarium Gastrodia Gastrodia anti-fungal protein Gastrodia elata gene overexpression Genetic engineering applications genetic resistance Genetics and breeding of economic plants Gossypium hirsutum Plant breeding: fundamental aspects and methodology plant pathogenic fungi pollen-tube pathway transformation transgenes transgenic coloured cotton transgenic plants Varietal selection. Specialized plant breeding, plant breeding aims Verticillium Verticillium dahliae Verticillium wilt |
| title | Over-expression of Gastrodia anti-fungal protein enhances Verticillium wilt resistance in coloured cotton |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T02%3A00%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Over-expression%20of%20Gastrodia%20anti-fungal%20protein%20enhances%20Verticillium%20wilt%20resistance%20in%20coloured%20cotton&rft.jtitle=Plant%20breeding&rft.au=Wang,%20Y.Q&rft.date=2004-10&rft.volume=123&rft.issue=5&rft.spage=454&rft.epage=459&rft.pages=454-459&rft.issn=0179-9541&rft.eissn=1439-0523&rft.coden=PLABED&rft_id=info:doi/10.1111/j.1439-0523.2004.01005.x&rft_dat=%3Cproquest_cross%3E17504094%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17504094&rft_id=info:pmid/&rfr_iscdi=true |