A simple method for monitoring chromatography column eluates for laccase activity during enzyme purification

A simple and economical method is described that allows rapid detection of laccase activity in chromatography column fractions during enzyme purification. Aliquots of column eluants are applied to filter paper coated with 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) containing a...

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Veröffentlicht in:	Biotechnology letters 2000-01, Vol.22 (2), p.105-108
Hauptverfasser:	DEKKER, R. F. H, KHU YEE LING, BARBOSA, A. M
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Enzyme engineering Fundamental and applied biological sciences. Psychology Improved methods for extraction and purification of enzymes Methods. Procedures. Technologies
Online-Zugang:	Volltext
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