Escherichia coli Vertebral Osteomyelitis Diagnosed According to Broad-range 16S rRNA Gene Polymerase Chain Reaction (PCR)
Identifying the causative agent of pyogenic osteomyelitis is often challenging, especially when antibiotics are administered before a biopsy. We herein present a case of osteomyelitis in the cervical vertebrae presenting with progressive paralytic symptoms, in which we successfully identified Escher...
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| Veröffentlicht in: | Internal Medicine 2015, Vol.54(24), pp.3237-3240 |
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| container_issue | 24 |
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| container_title | Internal Medicine |
| container_volume | 54 |
| creator | Shibata, Satoshi Tanizaki, Ryutaro Watanabe, Koji Makabe, Kenta Shoda, Naoki Kutsuna, Satoshi Nagamatsu, Maki Oka, Shinichi Ohmagari, Norio |
| description | Identifying the causative agent of pyogenic osteomyelitis is often challenging, especially when antibiotics are administered before a biopsy. We herein present a case of osteomyelitis in the cervical vertebrae presenting with progressive paralytic symptoms, in which we successfully identified Escherichia coli from a biopsy specimen using broad-range 16S rRNA gene polymerase chain reaction (PCR) even though sensitive antibiotics had been used for more than 50 days before the biopsy. Broad-range 16S rRNA gene PCR is a useful diagnostic method, especially when prebiopsy antibiotics are unavoidably used for a clinically unstable state. |
| doi_str_mv | 10.2169/internalmedicine.54.5066 |
| format | Article |
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Med.</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>54</volume><issue>24</issue><spage>3237</spage><epage>3240</epage><pages>3237-3240</pages><issn>0918-2918</issn><eissn>1349-7235</eissn><abstract>Identifying the causative agent of pyogenic osteomyelitis is often challenging, especially when antibiotics are administered before a biopsy. We herein present a case of osteomyelitis in the cervical vertebrae presenting with progressive paralytic symptoms, in which we successfully identified Escherichia coli from a biopsy specimen using broad-range 16S rRNA gene polymerase chain reaction (PCR) even though sensitive antibiotics had been used for more than 50 days before the biopsy. 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| ispartof | Internal Medicine, 2015, Vol.54(24), pp.3237-3240 |
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| source | J-STAGE Free; MEDLINE |
| subjects | a broad-range 16S rRNA gene PCR Anti-Bacterial Agents Cervical Vertebrae Enterobacteriaceae Infections - diagnosis Escherichia coli (E. coli) Escherichia coli - genetics Female Humans Middle Aged Osteomyelitis - diagnosis Polymerase Chain Reaction prebiopsy antibiotics exposure RNA, Ribosomal, 16S - genetics vertebral osteomyelitis |
| title | Escherichia coli Vertebral Osteomyelitis Diagnosed According to Broad-range 16S rRNA Gene Polymerase Chain Reaction (PCR) |
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