Development of Polymorphic Microsatellite Markers for the Killarney Fern (Vandenboschia speciosa, Hymenophyllaceae)
Premise of the study: We characterize 10 microsatellite loci in the endangered fern Vandenboschia speciosa (Hymenophyllaceae), enabling studies on the genetic population structure of this Macaronesian-European species using DNA hypervariable markers. Methods and Results: Ten primer sets were develop...
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| Veröffentlicht in: | Applications in plant sciences 2015-11, Vol.3 (11), p.n/a |
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| creator | García-López, M. del Carmen Schuler, Samira Ben-Menni López-Flores, Inmaculada Nieto-Lugilde, Marta Terrón-Camero, Laura Aguilera, Ismael Mazuecos Suárez-Santiago, Víctor N |
| description | Premise of the study: We characterize 10 microsatellite loci in the endangered fern Vandenboschia speciosa (Hymenophyllaceae), enabling studies on the genetic population structure of this Macaronesian-European species using DNA hypervariable markers. Methods and Results: Ten primer sets were developed and tested on 47 individuals in a total of two Iberian populations of V. speciosa. The primers amplified di- and hexanucelotide repeats. The number of alleles ranged from two to eight, and the expected heterozygosity ranged from 0.107 to 0.807 among the populations analyzed. Conclusions: The 10 microsatellite markers developed will be useful in characterizing the genetic diversity of V. speciosa and understanding its population structure (including the possible structure between sporophyte and gametophyte phases) and biogeographic history, and will provide important genetic data for the conservation of this species. |
| doi_str_mv | 10.3732/apps.1500067 |
| format | Article |
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Methods and Results: Ten primer sets were developed and tested on 47 individuals in a total of two Iberian populations of V. speciosa. The primers amplified di- and hexanucelotide repeats. The number of alleles ranged from two to eight, and the expected heterozygosity ranged from 0.107 to 0.807 among the populations analyzed. Conclusions: The 10 microsatellite markers developed will be useful in characterizing the genetic diversity of V. speciosa and understanding its population structure (including the possible structure between sporophyte and gametophyte phases) and biogeographic history, and will provide important genetic data for the conservation of this species.</description><identifier>ISSN: 2168-0450</identifier><identifier>EISSN: 2168-0450</identifier><identifier>DOI: 10.3732/apps.1500067</identifier><identifier>PMID: 26649267</identifier><language>eng</language><publisher>United States: Botanical Society of America</publisher><subject>Biogeography ; Cloning ; Deoxyribonucleic acid ; DNA ; endangered fern ; Ferns ; Genetic diversity ; Genetic markers ; Genetic testing ; Heterozygosity ; Hymenophyllaceae ; Laboratories ; microsatellite markers ; Microsatellites ; Plant sciences ; Population genetics ; Population structure ; Population studies ; PRIMER NOTES ; Primers ; Vandenboschia speciosa</subject><ispartof>Applications in plant sciences, 2015-11, Vol.3 (11), p.n/a</ispartof><rights>2015 The Author(s)</rights><rights>2015. This work is published under http://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b4387-cc9faed0c1cebaf06605e741d9cef0e2e4565e20d42b2ecd3067e65b622697a93</citedby><cites>FETCH-LOGICAL-b4387-cc9faed0c1cebaf06605e741d9cef0e2e4565e20d42b2ecd3067e65b622697a93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.3732/apps.1500067$$EPDF$$P50$$Gbioone$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.3732%2Fapps.1500067$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>109,314,780,784,864,1417,11562,27924,27925,45574,45575,46052,46476,52719</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26649267$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>García-López, M. del Carmen</creatorcontrib><creatorcontrib>Schuler, Samira Ben-Menni</creatorcontrib><creatorcontrib>López-Flores, Inmaculada</creatorcontrib><creatorcontrib>Nieto-Lugilde, Marta</creatorcontrib><creatorcontrib>Terrón-Camero, Laura</creatorcontrib><creatorcontrib>Aguilera, Ismael Mazuecos</creatorcontrib><creatorcontrib>Suárez-Santiago, Víctor N</creatorcontrib><title>Development of Polymorphic Microsatellite Markers for the Killarney Fern (Vandenboschia speciosa, Hymenophyllaceae)</title><title>Applications in plant sciences</title><addtitle>Appl Plant Sci</addtitle><description>Premise of the study: We characterize 10 microsatellite loci in the endangered fern Vandenboschia speciosa (Hymenophyllaceae), enabling studies on the genetic population structure of this Macaronesian-European species using DNA hypervariable markers. Methods and Results: Ten primer sets were developed and tested on 47 individuals in a total of two Iberian populations of V. speciosa. The primers amplified di- and hexanucelotide repeats. The number of alleles ranged from two to eight, and the expected heterozygosity ranged from 0.107 to 0.807 among the populations analyzed. Conclusions: The 10 microsatellite markers developed will be useful in characterizing the genetic diversity of V. speciosa and understanding its population structure (including the possible structure between sporophyte and gametophyte phases) and biogeographic history, and will provide important genetic data for the conservation of this species.</description><subject>Biogeography</subject><subject>Cloning</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>endangered fern</subject><subject>Ferns</subject><subject>Genetic diversity</subject><subject>Genetic markers</subject><subject>Genetic testing</subject><subject>Heterozygosity</subject><subject>Hymenophyllaceae</subject><subject>Laboratories</subject><subject>microsatellite markers</subject><subject>Microsatellites</subject><subject>Plant sciences</subject><subject>Population genetics</subject><subject>Population structure</subject><subject>Population studies</subject><subject>PRIMER NOTES</subject><subject>Primers</subject><subject>Vandenboschia speciosa</subject><issn>2168-0450</issn><issn>2168-0450</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kUtLxDAURoMoKurOtQTcqDiapmnSLAffqCj42IY0vWWibVOTjtJ_b4YZH7hwlRDOPdx8H0LbCTlKRUqPddeFoyQjhHCxhNZpwvMRYRlZ_nVfQ1shvESE5PFJslW0RjlnknKxjsIpvEPtugbaHrsK37t6aJzvJtbgW2u8C7qHurY94FvtX8EHXDmP-wnga1vX2rcw4HPwLd571m0JbeGCmViNQwfGxulDfDlEuesmQ8QNaNjfRCuVrgNsLc4N9HR-9nhyObq5u7g6Gd-MCpbmYmSMrDSUxCQGCl0RzkkGgiWlNFARoMAyngElJaMFBVOmMQPgWcEp5VJomW6gvbm38-5tCqFXjQ0m_ka34KZBJYJJTiiTIqK7f9AXN_Vt3E5RKonIuUjySB3OqVkuwUOlOm8b7QeVEDXrQ836UIs-Ir6zkE6LBspv-Cv9CGRz4MPWMPwrU-P7h_RHfDCfK6xzLfy_xSewvKVG</recordid><startdate>201511</startdate><enddate>201511</enddate><creator>García-López, M. del Carmen</creator><creator>Schuler, Samira Ben-Menni</creator><creator>López-Flores, Inmaculada</creator><creator>Nieto-Lugilde, Marta</creator><creator>Terrón-Camero, Laura</creator><creator>Aguilera, Ismael Mazuecos</creator><creator>Suárez-Santiago, Víctor N</creator><general>Botanical Society of America</general><general>John Wiley & Sons, Inc</general><scope>24P</scope><scope>WIN</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>201511</creationdate><title>Development of Polymorphic Microsatellite Markers for the Killarney Fern (Vandenboschia speciosa, Hymenophyllaceae)</title><author>García-López, M. del Carmen ; Schuler, Samira Ben-Menni ; López-Flores, Inmaculada ; Nieto-Lugilde, Marta ; Terrón-Camero, Laura ; Aguilera, Ismael Mazuecos ; Suárez-Santiago, Víctor N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b4387-cc9faed0c1cebaf06605e741d9cef0e2e4565e20d42b2ecd3067e65b622697a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Biogeography</topic><topic>Cloning</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>endangered fern</topic><topic>Ferns</topic><topic>Genetic diversity</topic><topic>Genetic markers</topic><topic>Genetic testing</topic><topic>Heterozygosity</topic><topic>Hymenophyllaceae</topic><topic>Laboratories</topic><topic>microsatellite markers</topic><topic>Microsatellites</topic><topic>Plant sciences</topic><topic>Population genetics</topic><topic>Population structure</topic><topic>Population studies</topic><topic>PRIMER NOTES</topic><topic>Primers</topic><topic>Vandenboschia speciosa</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>García-López, M. del Carmen</creatorcontrib><creatorcontrib>Schuler, Samira Ben-Menni</creatorcontrib><creatorcontrib>López-Flores, Inmaculada</creatorcontrib><creatorcontrib>Nieto-Lugilde, Marta</creatorcontrib><creatorcontrib>Terrón-Camero, Laura</creatorcontrib><creatorcontrib>Aguilera, Ismael Mazuecos</creatorcontrib><creatorcontrib>Suárez-Santiago, Víctor N</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>Wiley Online Library Free Content</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Applications in plant sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>García-López, M. del Carmen</au><au>Schuler, Samira Ben-Menni</au><au>López-Flores, Inmaculada</au><au>Nieto-Lugilde, Marta</au><au>Terrón-Camero, Laura</au><au>Aguilera, Ismael Mazuecos</au><au>Suárez-Santiago, Víctor N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of Polymorphic Microsatellite Markers for the Killarney Fern (Vandenboschia speciosa, Hymenophyllaceae)</atitle><jtitle>Applications in plant sciences</jtitle><addtitle>Appl Plant Sci</addtitle><date>2015-11</date><risdate>2015</risdate><volume>3</volume><issue>11</issue><epage>n/a</epage><issn>2168-0450</issn><eissn>2168-0450</eissn><abstract>Premise of the study: We characterize 10 microsatellite loci in the endangered fern Vandenboschia speciosa (Hymenophyllaceae), enabling studies on the genetic population structure of this Macaronesian-European species using DNA hypervariable markers. 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| subjects | Biogeography Cloning Deoxyribonucleic acid DNA endangered fern Ferns Genetic diversity Genetic markers Genetic testing Heterozygosity Hymenophyllaceae Laboratories microsatellite markers Microsatellites Plant sciences Population genetics Population structure Population studies PRIMER NOTES Primers Vandenboschia speciosa |
| title | Development of Polymorphic Microsatellite Markers for the Killarney Fern (Vandenboschia speciosa, Hymenophyllaceae) |
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