Virulence-related DNA sequences and adherence patterns in strains of enteropathogenic Escherichia coli
The presence of the genes for Escherichia coli adherence factor (EAF), attaching and effacing lesion ( eae) and bundle-forming pili ( bfp) in 72 strains identified as enteropathogenic E. coli (EPEC) by slide agglutination was evaluated using hybridization and PCR. The adherence property of these str...
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| Veröffentlicht in: | FEMS microbiology letters 2000-04, Vol.185 (1), p.89-93 |
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| creator | Bouzari, Saeid Jafari, Mohammad N Shokouhi, Forough Parsi, Mostafa Jafari, Anis |
| description | The presence of the genes for
Escherichia coli adherence factor (EAF), attaching and effacing lesion (
eae) and bundle-forming pili (
bfp) in 72 strains identified as enteropathogenic
E. coli (EPEC) by slide agglutination was evaluated using hybridization and PCR. The adherence property of these strains was assayed using 3h HeLa cells adherence assay. The results obtained indicated that virulence-associated genes were present in 65% of the strains but only ten (13.9%) isolates were positive for all the three markers (typical EPEC), 37 (51.4%) isolates carried either one or two of these determinants (atypical EPEC) and the remaining 25 (34.7%) were negative for all these genes. In vitro adherence assay showed that 44 (61.1%) strains adhered to HeLa cells with a defined pattern, 13 (18.1%) isolates adhered loosely with no definite pattern and the remaining 15 (20.8%) were non-adherent. Analysis of the results showed a statistically significant association between the presence of the virulence-related genes with adherence of the strains with a defined pattern (
P≤0.0001). These results indicated that since over 60% of the strains identified by serogrouping carried at least one of the putative virulence markers, it therefore seems that this simple test is still of value in our setting although the need for a confirmatory test is also indicated. |
| doi_str_mv | 10.1016/S0378-1097(00)00076-8 |
| format | Article |
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Escherichia coli adherence factor (EAF), attaching and effacing lesion (
eae) and bundle-forming pili (
bfp) in 72 strains identified as enteropathogenic
E. coli (EPEC) by slide agglutination was evaluated using hybridization and PCR. The adherence property of these strains was assayed using 3h HeLa cells adherence assay. The results obtained indicated that virulence-associated genes were present in 65% of the strains but only ten (13.9%) isolates were positive for all the three markers (typical EPEC), 37 (51.4%) isolates carried either one or two of these determinants (atypical EPEC) and the remaining 25 (34.7%) were negative for all these genes. In vitro adherence assay showed that 44 (61.1%) strains adhered to HeLa cells with a defined pattern, 13 (18.1%) isolates adhered loosely with no definite pattern and the remaining 15 (20.8%) were non-adherent. Analysis of the results showed a statistically significant association between the presence of the virulence-related genes with adherence of the strains with a defined pattern (
P≤0.0001). These results indicated that since over 60% of the strains identified by serogrouping carried at least one of the putative virulence markers, it therefore seems that this simple test is still of value in our setting although the need for a confirmatory test is also indicated.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1016/S0378-1097(00)00076-8</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>adherence factor ; Adhesion ; bfp gene ; eae gene ; Enteropathogenic Escherichia coli ; Escherichia coli ; Virulence marker</subject><ispartof>FEMS microbiology letters, 2000-04, Vol.185 (1), p.89-93</ispartof><rights>2000</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids></links><search><creatorcontrib>Bouzari, Saeid</creatorcontrib><creatorcontrib>Jafari, Mohammad N</creatorcontrib><creatorcontrib>Shokouhi, Forough</creatorcontrib><creatorcontrib>Parsi, Mostafa</creatorcontrib><creatorcontrib>Jafari, Anis</creatorcontrib><title>Virulence-related DNA sequences and adherence patterns in strains of enteropathogenic Escherichia coli</title><title>FEMS microbiology letters</title><description>The presence of the genes for
Escherichia coli adherence factor (EAF), attaching and effacing lesion (
eae) and bundle-forming pili (
bfp) in 72 strains identified as enteropathogenic
E. coli (EPEC) by slide agglutination was evaluated using hybridization and PCR. The adherence property of these strains was assayed using 3h HeLa cells adherence assay. The results obtained indicated that virulence-associated genes were present in 65% of the strains but only ten (13.9%) isolates were positive for all the three markers (typical EPEC), 37 (51.4%) isolates carried either one or two of these determinants (atypical EPEC) and the remaining 25 (34.7%) were negative for all these genes. In vitro adherence assay showed that 44 (61.1%) strains adhered to HeLa cells with a defined pattern, 13 (18.1%) isolates adhered loosely with no definite pattern and the remaining 15 (20.8%) were non-adherent. Analysis of the results showed a statistically significant association between the presence of the virulence-related genes with adherence of the strains with a defined pattern (
P≤0.0001). These results indicated that since over 60% of the strains identified by serogrouping carried at least one of the putative virulence markers, it therefore seems that this simple test is still of value in our setting although the need for a confirmatory test is also indicated.</description><subject>adherence factor</subject><subject>Adhesion</subject><subject>bfp gene</subject><subject>eae gene</subject><subject>Enteropathogenic Escherichia coli</subject><subject>Escherichia coli</subject><subject>Virulence marker</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNo9UMtOwzAQtBBIlMInIPmE4BBYO_EjJ1SV8pAqOPC4Wq69oUYhKXbK9-NQxGl3Z2dXM0PIKYNLBkxePUOpdMGgVucAFwCgZKH3yIQJVRWylnqfTP4ph-QopY9MqjjICWneQty22DksIrZ2QE9vHmc04dd2BBO1nafWrzGOI93YYcDYJRo6moZoQ277hmKX0T4v1_07dsHRRXL5JLh1sNT1bTgmB41tE5781Sl5vV28zO-L5dPdw3y2LJAD14VfgWM2S_Oi4UJZiXYlmC4r6RnWEhhjmqtVxRV60FqIBiss0SPn5crWopySs93fTeyzgzSYz5Actq3tsN8mw1RVC6jLTLzeETGr-Q4YTXJhtOhDRDcY3wfDwIz5mt98zRieATC_-Rpd_gDXYG-3</recordid><startdate>20000401</startdate><enddate>20000401</enddate><creator>Bouzari, Saeid</creator><creator>Jafari, Mohammad N</creator><creator>Shokouhi, Forough</creator><creator>Parsi, Mostafa</creator><creator>Jafari, Anis</creator><general>Elsevier B.V</general><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20000401</creationdate><title>Virulence-related DNA sequences and adherence patterns in strains of enteropathogenic Escherichia coli</title><author>Bouzari, Saeid ; Jafari, Mohammad N ; Shokouhi, Forough ; Parsi, Mostafa ; Jafari, Anis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e2028-db0c1a042d5f257a6eab518346d1e960111827b427ed08855fe4e3ede223ba953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>adherence factor</topic><topic>Adhesion</topic><topic>bfp gene</topic><topic>eae gene</topic><topic>Enteropathogenic Escherichia coli</topic><topic>Escherichia coli</topic><topic>Virulence marker</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bouzari, Saeid</creatorcontrib><creatorcontrib>Jafari, Mohammad N</creatorcontrib><creatorcontrib>Shokouhi, Forough</creatorcontrib><creatorcontrib>Parsi, Mostafa</creatorcontrib><creatorcontrib>Jafari, Anis</creatorcontrib><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bouzari, Saeid</au><au>Jafari, Mohammad N</au><au>Shokouhi, Forough</au><au>Parsi, Mostafa</au><au>Jafari, Anis</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Virulence-related DNA sequences and adherence patterns in strains of enteropathogenic Escherichia coli</atitle><jtitle>FEMS microbiology letters</jtitle><date>2000-04-01</date><risdate>2000</risdate><volume>185</volume><issue>1</issue><spage>89</spage><epage>93</epage><pages>89-93</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><abstract>The presence of the genes for
Escherichia coli adherence factor (EAF), attaching and effacing lesion (
eae) and bundle-forming pili (
bfp) in 72 strains identified as enteropathogenic
E. coli (EPEC) by slide agglutination was evaluated using hybridization and PCR. The adherence property of these strains was assayed using 3h HeLa cells adherence assay. The results obtained indicated that virulence-associated genes were present in 65% of the strains but only ten (13.9%) isolates were positive for all the three markers (typical EPEC), 37 (51.4%) isolates carried either one or two of these determinants (atypical EPEC) and the remaining 25 (34.7%) were negative for all these genes. In vitro adherence assay showed that 44 (61.1%) strains adhered to HeLa cells with a defined pattern, 13 (18.1%) isolates adhered loosely with no definite pattern and the remaining 15 (20.8%) were non-adherent. Analysis of the results showed a statistically significant association between the presence of the virulence-related genes with adherence of the strains with a defined pattern (
P≤0.0001). These results indicated that since over 60% of the strains identified by serogrouping carried at least one of the putative virulence markers, it therefore seems that this simple test is still of value in our setting although the need for a confirmatory test is also indicated.</abstract><pub>Elsevier B.V</pub><doi>10.1016/S0378-1097(00)00076-8</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | FEMS microbiology letters, 2000-04, Vol.185 (1), p.89-93 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17495093 |
| source | Wiley Online Library Journals Frontfile Complete; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | adherence factor Adhesion bfp gene eae gene Enteropathogenic Escherichia coli Escherichia coli Virulence marker |
| title | Virulence-related DNA sequences and adherence patterns in strains of enteropathogenic Escherichia coli |
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