A plate assay for simultaneous screening of polysaccharide‐ and protein‐degrading micro‐organisms

Aims:  To develop a plate assay for simultaneous screening of polysaccharide‐degrading and protein‐degrading micro‐organisms. Methods and Results:  A plate assay, based on the visible solubilization of small substrate particles and the formation of haloes on Petri dishes, containing a mixture of div...

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Veröffentlicht in:Letters in applied microbiology 2005-01, Vol.40 (2), p.92-98
Hauptverfasser: Ten, L.N., Im, W.‐T., Kim, M.‐K., Lee, S.‐T.
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container_issue 2
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container_title Letters in applied microbiology
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creator Ten, L.N.
Im, W.‐T.
Kim, M.‐K.
Lee, S.‐T.
description Aims:  To develop a plate assay for simultaneous screening of polysaccharide‐degrading and protein‐degrading micro‐organisms. Methods and Results:  A plate assay, based on the visible solubilization of small substrate particles and the formation of haloes on Petri dishes, containing a mixture of diversely coloured insoluble polysaccharides and dye‐labelled collagen as chromogenic substrates, was developed. This method was successfully applied for isolating the diverse polysaccharide‐ and/or protein‐degrading bacteria from soil and sludge samples. Selected strains were identified using 16S rDNA partial sequencing; most of them belong to the genera Bacillus, Cellulomonas and Cellulosimicrobium. Conclusions:  This novel approach provides unique and valuable information for direct primary screening when the target of selection is micro‐organisms exhibiting protein‐degrading activity, polysaccharide‐degrading activity or a specific combination of them. Significance and Impact of the Study:  This plate assay is convenient and easy to perform, rapid, and more adaptable for screening of a large number of samples, compared with other existing methods in the literature.
doi_str_mv 10.1111/j.1472-765X.2004.01637.x
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Methods and Results:  A plate assay, based on the visible solubilization of small substrate particles and the formation of haloes on Petri dishes, containing a mixture of diversely coloured insoluble polysaccharides and dye‐labelled collagen as chromogenic substrates, was developed. This method was successfully applied for isolating the diverse polysaccharide‐ and/or protein‐degrading bacteria from soil and sludge samples. Selected strains were identified using 16S rDNA partial sequencing; most of them belong to the genera Bacillus, Cellulomonas and Cellulosimicrobium. Conclusions:  This novel approach provides unique and valuable information for direct primary screening when the target of selection is micro‐organisms exhibiting protein‐degrading activity, polysaccharide‐degrading activity or a specific combination of them. 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Methods and Results:  A plate assay, based on the visible solubilization of small substrate particles and the formation of haloes on Petri dishes, containing a mixture of diversely coloured insoluble polysaccharides and dye‐labelled collagen as chromogenic substrates, was developed. This method was successfully applied for isolating the diverse polysaccharide‐ and/or protein‐degrading bacteria from soil and sludge samples. Selected strains were identified using 16S rDNA partial sequencing; most of them belong to the genera Bacillus, Cellulomonas and Cellulosimicrobium. Conclusions:  This novel approach provides unique and valuable information for direct primary screening when the target of selection is micro‐organisms exhibiting protein‐degrading activity, polysaccharide‐degrading activity or a specific combination of them. Significance and Impact of the Study:  This plate assay is convenient and easy to perform, rapid, and more adaptable for screening of a large number of samples, compared with other existing methods in the literature.</description><subject>Actinomycetales - classification</subject><subject>Actinomycetales - genetics</subject><subject>Actinomycetales - isolation &amp; purification</subject><subject>Actinomycetales - metabolism</subject><subject>Bacillus</subject><subject>Bacillus - classification</subject><subject>Bacillus - genetics</subject><subject>Bacillus - isolation &amp; purification</subject><subject>Bacillus - metabolism</subject><subject>Bacteria - classification</subject><subject>Bacteria - enzymology</subject><subject>Bacteria - genetics</subject><subject>Bacteria - isolation &amp; purification</subject><subject>Bacteriological Techniques</subject><subject>Biodegradation, Environmental</subject><subject>Biological and medical sciences</subject><subject>Cellulomonas</subject><subject>Cellulomonas - classification</subject><subject>Cellulomonas - genetics</subject><subject>Cellulomonas - isolation &amp; purification</subject><subject>Cellulomonas - metabolism</subject><subject>Cellulosimicrobium</subject><subject>Chromogenic Compounds</subject><subject>chromogenic substrates</subject><subject>collagen</subject><subject>Collagen - metabolism</subject><subject>Coloring Agents - metabolism</subject><subject>Culture Media</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>microbial polysaccharases</subject><subject>microbial proteases</subject><subject>Microbiology</subject><subject>polysaccharides</subject><subject>Polysaccharides - metabolism</subject><subject>Proteins - metabolism</subject><subject>Sewage - microbiology</subject><subject>Soil Microbiology</subject><issn>0266-8254</issn><issn>1472-765X</issn><issn>1365-2673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMtuEzEUhi0EoqHtK1TelN0Mvo09s-giqqAgBbEBqTvrjO1JHc0l-CSi2fEIPCNPUk8T0S3e-PYd-z8fIZSzkufxYVNyZURhdHVfCsZUybiWpnx8RRb_Ll6TBRNaF7Wo1Bl5h7hhjNVcNG_JGa-0UpzpBVkv6baHXaCACAfaTYliHPb9DsYw7ZGiSyGMcVzTqaPbqT8gOPcAKfrw9_cfCqOn2zTtQhzz1od1Aj_DQ3RpyidTWsMYccAL8qaDHsPlaT4nPz59_H77uVh9u_tyu1wVTpnaFKHxJjcklfJd1UrhBHjJdWhASSkaUTvGfdeC8pq3jjslmWONMdpzLXxr5Dl5f3w3p_q5D7izQ0QX-v7Yj-VGNVxzlcH6COagiCl0dpviAOlgObOzZLuxs0s7u7SzZPss2T7m0qvTH_t2CP6l8GQ1A9cnANBB3yUYXcQXTleaMSMyd3PkfsU-HP47gF0tv84r-QTRV5xu</recordid><startdate>20050101</startdate><enddate>20050101</enddate><creator>Ten, L.N.</creator><creator>Im, W.‐T.</creator><creator>Kim, M.‐K.</creator><creator>Lee, S.‐T.</creator><general>Blackwell Science Ltd</general><general>Blackwell Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20050101</creationdate><title>A plate assay for simultaneous screening of polysaccharide‐ and protein‐degrading micro‐organisms</title><author>Ten, L.N. ; Im, W.‐T. ; Kim, M.‐K. ; Lee, S.‐T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4787-e9d7200344df5b32c2ad316e9a4332928c01dfba4d61bc1c430c09776d162db73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Actinomycetales - classification</topic><topic>Actinomycetales - genetics</topic><topic>Actinomycetales - isolation &amp; purification</topic><topic>Actinomycetales - metabolism</topic><topic>Bacillus</topic><topic>Bacillus - classification</topic><topic>Bacillus - genetics</topic><topic>Bacillus - isolation &amp; purification</topic><topic>Bacillus - metabolism</topic><topic>Bacteria - classification</topic><topic>Bacteria - enzymology</topic><topic>Bacteria - genetics</topic><topic>Bacteria - isolation &amp; purification</topic><topic>Bacteriological Techniques</topic><topic>Biodegradation, Environmental</topic><topic>Biological and medical sciences</topic><topic>Cellulomonas</topic><topic>Cellulomonas - classification</topic><topic>Cellulomonas - genetics</topic><topic>Cellulomonas - isolation &amp; purification</topic><topic>Cellulomonas - metabolism</topic><topic>Cellulosimicrobium</topic><topic>Chromogenic Compounds</topic><topic>chromogenic substrates</topic><topic>collagen</topic><topic>Collagen - metabolism</topic><topic>Coloring Agents - metabolism</topic><topic>Culture Media</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>microbial polysaccharases</topic><topic>microbial proteases</topic><topic>Microbiology</topic><topic>polysaccharides</topic><topic>Polysaccharides - metabolism</topic><topic>Proteins - metabolism</topic><topic>Sewage - microbiology</topic><topic>Soil Microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ten, L.N.</creatorcontrib><creatorcontrib>Im, W.‐T.</creatorcontrib><creatorcontrib>Kim, M.‐K.</creatorcontrib><creatorcontrib>Lee, S.‐T.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Letters in applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ten, L.N.</au><au>Im, W.‐T.</au><au>Kim, M.‐K.</au><au>Lee, S.‐T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A plate assay for simultaneous screening of polysaccharide‐ and protein‐degrading micro‐organisms</atitle><jtitle>Letters in applied microbiology</jtitle><addtitle>Lett Appl Microbiol</addtitle><date>2005-01-01</date><risdate>2005</risdate><volume>40</volume><issue>2</issue><spage>92</spage><epage>98</epage><pages>92-98</pages><issn>0266-8254</issn><eissn>1472-765X</eissn><eissn>1365-2673</eissn><coden>LAMIE7</coden><abstract>Aims:  To develop a plate assay for simultaneous screening of polysaccharide‐degrading and protein‐degrading micro‐organisms. Methods and Results:  A plate assay, based on the visible solubilization of small substrate particles and the formation of haloes on Petri dishes, containing a mixture of diversely coloured insoluble polysaccharides and dye‐labelled collagen as chromogenic substrates, was developed. This method was successfully applied for isolating the diverse polysaccharide‐ and/or protein‐degrading bacteria from soil and sludge samples. Selected strains were identified using 16S rDNA partial sequencing; most of them belong to the genera Bacillus, Cellulomonas and Cellulosimicrobium. Conclusions:  This novel approach provides unique and valuable information for direct primary screening when the target of selection is micro‐organisms exhibiting protein‐degrading activity, polysaccharide‐degrading activity or a specific combination of them. Significance and Impact of the Study:  This plate assay is convenient and easy to perform, rapid, and more adaptable for screening of a large number of samples, compared with other existing methods in the literature.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>15644106</pmid><doi>10.1111/j.1472-765X.2004.01637.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record>
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source MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals; Alma/SFX Local Collection
subjects Actinomycetales - classification
Actinomycetales - genetics
Actinomycetales - isolation & purification
Actinomycetales - metabolism
Bacillus
Bacillus - classification
Bacillus - genetics
Bacillus - isolation & purification
Bacillus - metabolism
Bacteria - classification
Bacteria - enzymology
Bacteria - genetics
Bacteria - isolation & purification
Bacteriological Techniques
Biodegradation, Environmental
Biological and medical sciences
Cellulomonas
Cellulomonas - classification
Cellulomonas - genetics
Cellulomonas - isolation & purification
Cellulomonas - metabolism
Cellulosimicrobium
Chromogenic Compounds
chromogenic substrates
collagen
Collagen - metabolism
Coloring Agents - metabolism
Culture Media
Fundamental and applied biological sciences. Psychology
microbial polysaccharases
microbial proteases
Microbiology
polysaccharides
Polysaccharides - metabolism
Proteins - metabolism
Sewage - microbiology
Soil Microbiology
title A plate assay for simultaneous screening of polysaccharide‐ and protein‐degrading micro‐organisms
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