Co-localization of Patched and activated Sonic hedgehog to lysosomes in neurons
We demonstrate co-localization of the Patched 1 (Ptcl) receptor and its ligand sonic hedgehog (Shh) in lysosomes suggests an intracellular sorting mechanism for this receptor and its ligand. Treatment of murine brain primary cultures and a human teratoma cell line with the N-terminal activated form...
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| Veröffentlicht in: | Neuroreport 2000-02, Vol.11 (3), p.581-585 |
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| creator | Mastronardi, Fabrizio G Dimitroulakos, Jim Kamel-Reid, Suzanne Manoukian, Armen S |
| description | We demonstrate co-localization of the Patched 1 (Ptcl) receptor and its ligand sonic hedgehog (Shh) in lysosomes suggests an intracellular sorting mechanism for this receptor and its ligand. Treatment of murine brain primary cultures and a human teratoma cell line with the N-terminal activated form of Shh (ShhNT), a Ptc1-Shh complex was observed in lysosomes. Consistent with this interaction, Western immuno-blot analysis revealed intracellular localization of native Ptc1 and ShhNT. Examination of the topological model of the Ptc1 receptor revealed a number of Yxx lysosomal targeting sequences consistent with our observations for Ptc1 sorting. |
| doi_str_mv | 10.1097/00001756-200002280-00030 |
| format | Article |
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Psychology</subject><subject>Hedgehog Proteins</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Intracellular Signaling Peptides and Proteins</subject><subject>Membrane Proteins - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Neurons - metabolism</subject><subject>Patched protein</subject><subject>Patched Receptors</subject><subject>Patched-1 Receptor</subject><subject>protein trafficking</subject><subject>Proteins - metabolism</subject><subject>Receptors, Cell Surface</subject><subject>sonic hedgehog protein</subject><subject>Subcellular Fractions - metabolism</subject><subject>teratoma cells</subject><subject>Tissue Distribution</subject><subject>Trans-Activators</subject><subject>Tumor Cells, Cultured</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0959-4965</issn><issn>1473-558X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kd9rFDEQgINY7Fn9FyQP4ttqssluJo9yWBUKFargW5jLTnqruU1Ndi31r2_OO3-8dCDMZPhmAl8Y41K8lsKaN6KGNF3ftPuqbUE0NSvxiK2kNqrpOvj6mK2E7Wyjbd-dsqelfKuIFRKesFMpjAQlYcUu16mJyWMcf-E8pomnwD_h7Lc0cJzq8fP4E-d6u0rT6HntX9M2XfM58XhXUkk7Knyc-ERLTlN5xk4CxkLPj_mMfTl_93n9obm4fP9x_fai8Vr2ouk9amUCdgLQyxAIhlaDAtTdRotNq2gIuLFArUUwQ4_QkmnBQDBWe-jVGXt12HuT04-Fyux2Y_EUI06UluKk0WCl3INwAH1OpWQK7iaPO8x3Tgq3l-n-yHR_ZbrfMuvoi-Mby2ZHw3-DB3sVeHkEsFSFIePkx_KPU8KCMBXTB-w2xZly-R6XW8puSxjnrXvoL9U9FaGLlQ</recordid><startdate>20000228</startdate><enddate>20000228</enddate><creator>Mastronardi, Fabrizio G</creator><creator>Dimitroulakos, Jim</creator><creator>Kamel-Reid, Suzanne</creator><creator>Manoukian, Armen S</creator><general>Lippincott Williams & Wilkins, Inc</general><general>Lippincott Williams and Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>20000228</creationdate><title>Co-localization of Patched and activated Sonic hedgehog to lysosomes in neurons</title><author>Mastronardi, Fabrizio G ; Dimitroulakos, Jim ; Kamel-Reid, Suzanne ; Manoukian, Armen S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4160-6ca437fa508ac1ffe8d24838a45b40b23edfab98e29a87d6a82e72878f794c863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Biochemistry and metabolism</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Brain - metabolism</topic><topic>Cell Membrane - metabolism</topic><topic>Central nervous system</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hedgehog Proteins</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Intracellular Signaling Peptides and Proteins</topic><topic>Membrane Proteins - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Neurons - metabolism</topic><topic>Patched protein</topic><topic>Patched Receptors</topic><topic>Patched-1 Receptor</topic><topic>protein trafficking</topic><topic>Proteins - metabolism</topic><topic>Receptors, Cell Surface</topic><topic>sonic hedgehog protein</topic><topic>Subcellular Fractions - metabolism</topic><topic>teratoma cells</topic><topic>Tissue Distribution</topic><topic>Trans-Activators</topic><topic>Tumor Cells, Cultured</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mastronardi, Fabrizio G</creatorcontrib><creatorcontrib>Dimitroulakos, Jim</creatorcontrib><creatorcontrib>Kamel-Reid, Suzanne</creatorcontrib><creatorcontrib>Manoukian, Armen S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Neuroreport</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mastronardi, Fabrizio G</au><au>Dimitroulakos, Jim</au><au>Kamel-Reid, Suzanne</au><au>Manoukian, Armen S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Co-localization of Patched and activated Sonic hedgehog to lysosomes in neurons</atitle><jtitle>Neuroreport</jtitle><addtitle>Neuroreport</addtitle><date>2000-02-28</date><risdate>2000</risdate><volume>11</volume><issue>3</issue><spage>581</spage><epage>585</epage><pages>581-585</pages><issn>0959-4965</issn><eissn>1473-558X</eissn><abstract>We demonstrate co-localization of the Patched 1 (Ptcl) receptor and its ligand sonic hedgehog (Shh) in lysosomes suggests an intracellular sorting mechanism for this receptor and its ligand. Treatment of murine brain primary cultures and a human teratoma cell line with the N-terminal activated form of Shh (ShhNT), a Ptc1-Shh complex was observed in lysosomes. Consistent with this interaction, Western immuno-blot analysis revealed intracellular localization of native Ptc1 and ShhNT. Examination of the topological model of the Ptc1 receptor revealed a number of Yxx lysosomal targeting sequences consistent with our observations for Ptc1 sorting.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins, Inc</pub><pmid>10718318</pmid><doi>10.1097/00001756-200002280-00030</doi><tpages>5</tpages></addata></record> |
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| subjects | Animals Biochemistry and metabolism Biological and medical sciences Blotting, Western Brain - metabolism Cell Membrane - metabolism Central nervous system Fundamental and applied biological sciences. Psychology Hedgehog Proteins Humans Immunohistochemistry Intracellular Signaling Peptides and Proteins Membrane Proteins - metabolism Mice Mice, Inbred Strains Neurons - metabolism Patched protein Patched Receptors Patched-1 Receptor protein trafficking Proteins - metabolism Receptors, Cell Surface sonic hedgehog protein Subcellular Fractions - metabolism teratoma cells Tissue Distribution Trans-Activators Tumor Cells, Cultured Vertebrates: nervous system and sense organs |
| title | Co-localization of Patched and activated Sonic hedgehog to lysosomes in neurons |
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