nuclear localization signal and the C-terminal region of FHY1 are required for transmission of phytochrome A signals
Plants use the family of phytochrome photoreceptors to sense their light environment in the red/far-red region of the spectrum. Phytochrome A (phyA) is the primary photoreceptor that regulates germination and early seedling development. This phytochrome mediates seedling de-etiolation for the develo...
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| Veröffentlicht in: | The Plant journal : for cell and molecular biology 2004-11, Vol.40 (3), p.355-365 |
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| creator | Zeidler, M Zhou, Q Sarda, X Yau, C.P Chua, N.H |
| description | Plants use the family of phytochrome photoreceptors to sense their light environment in the red/far-red region of the spectrum. Phytochrome A (phyA) is the primary photoreceptor that regulates germination and early seedling development. This phytochrome mediates seedling de-etiolation for the developmental transition from heterotrophic to photoauxotrophic growth. High intensity far-red light provides a way to specifically assess the role of phyA in this process and was used to isolate phyA-signaling intermediates. fhy1 and pat3 (renamed fhy1-3) are independently isolated alleles of a gene encoding a phyA signal transduction component. FHY1 is a small 24 kDa protein that shows no homology to known functional motifs, besides a small conserved septin-related domain at the C-terminus, a putative nuclear localization signal (NLS) and a putative nuclear exclusion signal (NES). Here we demonstrate that the septin-related domain is important for FHY1 to transmit phyA signals. Moreover, the putative NLS and NES of FHY1 are indeed involved in its nuclear localization and exclusion. Nuclear localization of FHY1 is needed for it to execute responses downstream of phyA. Together with the results from global expression analysis, our findings point to an important role of FHY1 in phyA signaling through its nuclear translocation and induction of gene expression. |
| doi_str_mv | 10.1111/j.1365-313x.2004.02212.x |
| format | Article |
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Phytochrome A (phyA) is the primary photoreceptor that regulates germination and early seedling development. This phytochrome mediates seedling de-etiolation for the developmental transition from heterotrophic to photoauxotrophic growth. High intensity far-red light provides a way to specifically assess the role of phyA in this process and was used to isolate phyA-signaling intermediates. fhy1 and pat3 (renamed fhy1-3) are independently isolated alleles of a gene encoding a phyA signal transduction component. FHY1 is a small 24 kDa protein that shows no homology to known functional motifs, besides a small conserved septin-related domain at the C-terminus, a putative nuclear localization signal (NLS) and a putative nuclear exclusion signal (NES). Here we demonstrate that the septin-related domain is important for FHY1 to transmit phyA signals. Moreover, the putative NLS and NES of FHY1 are indeed involved in its nuclear localization and exclusion. Nuclear localization of FHY1 is needed for it to execute responses downstream of phyA. Together with the results from global expression analysis, our findings point to an important role of FHY1 in phyA signaling through its nuclear translocation and induction of gene expression.</description><identifier>ISSN: 0960-7412</identifier><identifier>EISSN: 1365-313X</identifier><identifier>DOI: 10.1111/j.1365-313x.2004.02212.x</identifier><identifier>PMID: 15469493</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Amino Acid Sequence ; amino acid sequences ; Arabidopsis ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis Proteins - chemistry ; Arabidopsis Proteins - metabolism ; Arabidopsis Proteins - physiology ; Arabidopsis thaliana ; Biological and medical sciences ; Cell biochemistry ; cell nucleus ; Cell Nucleus - metabolism ; Cell physiology ; Conserved Sequence ; conserved sequences ; Dose-Response Relationship, Radiation ; FHY1 ; Fundamental and applied biological sciences. Psychology ; Gene expression ; gene expression regulation ; Gene Expression Regulation, Plant ; Light ; localization ; Molecular Sequence Data ; nuclear localization signal ; Nuclear Localization Signals ; nuclear proteins ; nucleotide sequences ; PAT3 ; photoreceptors ; phytochrome ; Phytochrome - chemistry ; Phytochrome - metabolism ; Phytochrome - physiology ; Phytochrome A ; Plant growth ; Plant physiology and development ; Plant populations ; Plants, Genetically Modified ; Seeds ; Sequence Alignment ; Sequence Homology, Amino Acid ; Signal Transduction ; Species Specificity ; structure-activity relationships</subject><ispartof>The Plant journal : for cell and molecular biology, 2004-11, Vol.40 (3), p.355-365</ispartof><rights>2004 INIST-CNRS</rights><rights>2004 Blackwell Publishing Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6222-d63dc95337b89626b7425fdef80bbe307b435dab1fcf54f221d7c3488917b54b3</citedby><cites>FETCH-LOGICAL-c6222-d63dc95337b89626b7425fdef80bbe307b435dab1fcf54f221d7c3488917b54b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-313X.2004.02212.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-313X.2004.02212.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16188849$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15469493$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zeidler, M</creatorcontrib><creatorcontrib>Zhou, Q</creatorcontrib><creatorcontrib>Sarda, X</creatorcontrib><creatorcontrib>Yau, C.P</creatorcontrib><creatorcontrib>Chua, N.H</creatorcontrib><title>nuclear localization signal and the C-terminal region of FHY1 are required for transmission of phytochrome A signals</title><title>The Plant journal : for cell and molecular biology</title><addtitle>Plant J</addtitle><description>Plants use the family of phytochrome photoreceptors to sense their light environment in the red/far-red region of the spectrum. Phytochrome A (phyA) is the primary photoreceptor that regulates germination and early seedling development. This phytochrome mediates seedling de-etiolation for the developmental transition from heterotrophic to photoauxotrophic growth. High intensity far-red light provides a way to specifically assess the role of phyA in this process and was used to isolate phyA-signaling intermediates. fhy1 and pat3 (renamed fhy1-3) are independently isolated alleles of a gene encoding a phyA signal transduction component. FHY1 is a small 24 kDa protein that shows no homology to known functional motifs, besides a small conserved septin-related domain at the C-terminus, a putative nuclear localization signal (NLS) and a putative nuclear exclusion signal (NES). Here we demonstrate that the septin-related domain is important for FHY1 to transmit phyA signals. Moreover, the putative NLS and NES of FHY1 are indeed involved in its nuclear localization and exclusion. Nuclear localization of FHY1 is needed for it to execute responses downstream of phyA. Together with the results from global expression analysis, our findings point to an important role of FHY1 in phyA signaling through its nuclear translocation and induction of gene expression.</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Arabidopsis</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - chemistry</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Arabidopsis Proteins - physiology</subject><subject>Arabidopsis thaliana</subject><subject>Biological and medical sciences</subject><subject>Cell biochemistry</subject><subject>cell nucleus</subject><subject>Cell Nucleus - metabolism</subject><subject>Cell physiology</subject><subject>Conserved Sequence</subject><subject>conserved sequences</subject><subject>Dose-Response Relationship, Radiation</subject><subject>FHY1</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>gene expression regulation</subject><subject>Gene Expression Regulation, Plant</subject><subject>Light</subject><subject>localization</subject><subject>Molecular Sequence Data</subject><subject>nuclear localization signal</subject><subject>Nuclear Localization Signals</subject><subject>nuclear proteins</subject><subject>nucleotide sequences</subject><subject>PAT3</subject><subject>photoreceptors</subject><subject>phytochrome</subject><subject>Phytochrome - chemistry</subject><subject>Phytochrome - metabolism</subject><subject>Phytochrome - physiology</subject><subject>Phytochrome A</subject><subject>Plant growth</subject><subject>Plant physiology and development</subject><subject>Plant populations</subject><subject>Plants, Genetically Modified</subject><subject>Seeds</subject><subject>Sequence Alignment</subject><subject>Sequence Homology, Amino Acid</subject><subject>Signal Transduction</subject><subject>Species Specificity</subject><subject>structure-activity relationships</subject><issn>0960-7412</issn><issn>1365-313X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkV-L1DAUxYMo7jj6FTQI-taaf03SBx-WwXWVBQV3QZ9CkiYzHdpmNmlxxk9v6lQWfDIvN9z8zs3lHAAgRiXO592-xJRXBcX0WBKEWIkIwaQ8PgKrvw_fH4MVqjkqBMPkAjxLaY8QFpSzp-ACV4zXrKYrMA6T7ZyOsAtWd-0vPbZhgKndDrqDemjguHNwU4wu9u3cim47A8HDq-sfGOrocut-aqNroA8RjlEPqW9TWqjD7jQGu4uhd_BymZuegyc-F_diqWtwd_XhdnNd3Hz5-GlzeVNYTggpGk4bW1eUCiNrTrgRjFS-cV4iYxxFwjBaNdpgb33FfLagEZYyKWssTMUMXYO357mHGO4nl0aVN7Ou6_TgwpQUFkwKKVgGX_8D7sMU51UVwZTlg3iG5BmyMaQUnVeH2PY6nhRGao5F7dXsvprdV3Ms6k8s6pilL5f5k-ld8yBccsjAmwXQKefgs4u2TQ8cx1LKDK7B-zP3s-3c6b8XULdfP8-3rH911nsdlN7G_MfdN4IwRaiuePaC_gbee7Is</recordid><startdate>200411</startdate><enddate>200411</enddate><creator>Zeidler, M</creator><creator>Zhou, Q</creator><creator>Sarda, X</creator><creator>Yau, C.P</creator><creator>Chua, N.H</creator><general>Blackwell Science Ltd</general><general>Blackwell Science</general><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>200411</creationdate><title>nuclear localization signal and the C-terminal region of FHY1 are required for transmission of phytochrome A signals</title><author>Zeidler, M ; Zhou, Q ; Sarda, X ; Yau, C.P ; Chua, N.H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6222-d63dc95337b89626b7425fdef80bbe307b435dab1fcf54f221d7c3488917b54b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Arabidopsis</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins - chemistry</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Arabidopsis Proteins - physiology</topic><topic>Arabidopsis thaliana</topic><topic>Biological and medical sciences</topic><topic>Cell biochemistry</topic><topic>cell nucleus</topic><topic>Cell Nucleus - metabolism</topic><topic>Cell physiology</topic><topic>Conserved Sequence</topic><topic>conserved sequences</topic><topic>Dose-Response Relationship, Radiation</topic><topic>FHY1</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>gene expression regulation</topic><topic>Gene Expression Regulation, Plant</topic><topic>Light</topic><topic>localization</topic><topic>Molecular Sequence Data</topic><topic>nuclear localization signal</topic><topic>Nuclear Localization Signals</topic><topic>nuclear proteins</topic><topic>nucleotide sequences</topic><topic>PAT3</topic><topic>photoreceptors</topic><topic>phytochrome</topic><topic>Phytochrome - chemistry</topic><topic>Phytochrome - metabolism</topic><topic>Phytochrome - physiology</topic><topic>Phytochrome A</topic><topic>Plant growth</topic><topic>Plant physiology and development</topic><topic>Plant populations</topic><topic>Plants, Genetically Modified</topic><topic>Seeds</topic><topic>Sequence Alignment</topic><topic>Sequence Homology, Amino Acid</topic><topic>Signal Transduction</topic><topic>Species Specificity</topic><topic>structure-activity relationships</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zeidler, M</creatorcontrib><creatorcontrib>Zhou, Q</creatorcontrib><creatorcontrib>Sarda, X</creatorcontrib><creatorcontrib>Yau, C.P</creatorcontrib><creatorcontrib>Chua, N.H</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>The Plant journal : for cell and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zeidler, M</au><au>Zhou, Q</au><au>Sarda, X</au><au>Yau, C.P</au><au>Chua, N.H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>nuclear localization signal and the C-terminal region of FHY1 are required for transmission of phytochrome A signals</atitle><jtitle>The Plant journal : for cell and molecular biology</jtitle><addtitle>Plant J</addtitle><date>2004-11</date><risdate>2004</risdate><volume>40</volume><issue>3</issue><spage>355</spage><epage>365</epage><pages>355-365</pages><issn>0960-7412</issn><eissn>1365-313X</eissn><abstract>Plants use the family of phytochrome photoreceptors to sense their light environment in the red/far-red region of the spectrum. Phytochrome A (phyA) is the primary photoreceptor that regulates germination and early seedling development. This phytochrome mediates seedling de-etiolation for the developmental transition from heterotrophic to photoauxotrophic growth. High intensity far-red light provides a way to specifically assess the role of phyA in this process and was used to isolate phyA-signaling intermediates. fhy1 and pat3 (renamed fhy1-3) are independently isolated alleles of a gene encoding a phyA signal transduction component. FHY1 is a small 24 kDa protein that shows no homology to known functional motifs, besides a small conserved septin-related domain at the C-terminus, a putative nuclear localization signal (NLS) and a putative nuclear exclusion signal (NES). Here we demonstrate that the septin-related domain is important for FHY1 to transmit phyA signals. Moreover, the putative NLS and NES of FHY1 are indeed involved in its nuclear localization and exclusion. Nuclear localization of FHY1 is needed for it to execute responses downstream of phyA. Together with the results from global expression analysis, our findings point to an important role of FHY1 in phyA signaling through its nuclear translocation and induction of gene expression.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>15469493</pmid><doi>10.1111/j.1365-313x.2004.02212.x</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Sequence amino acid sequences Arabidopsis Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis Proteins - chemistry Arabidopsis Proteins - metabolism Arabidopsis Proteins - physiology Arabidopsis thaliana Biological and medical sciences Cell biochemistry cell nucleus Cell Nucleus - metabolism Cell physiology Conserved Sequence conserved sequences Dose-Response Relationship, Radiation FHY1 Fundamental and applied biological sciences. Psychology Gene expression gene expression regulation Gene Expression Regulation, Plant Light localization Molecular Sequence Data nuclear localization signal Nuclear Localization Signals nuclear proteins nucleotide sequences PAT3 photoreceptors phytochrome Phytochrome - chemistry Phytochrome - metabolism Phytochrome - physiology Phytochrome A Plant growth Plant physiology and development Plant populations Plants, Genetically Modified Seeds Sequence Alignment Sequence Homology, Amino Acid Signal Transduction Species Specificity structure-activity relationships |
| title | nuclear localization signal and the C-terminal region of FHY1 are required for transmission of phytochrome A signals |
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