Pulchellin, a highly toxic type 2 ribosome-inactivating protein from Abrus pulchellus. Cloning heterologous expression of A-chain and structural studies

Pulchellin is a type 2 ribosome-inactivating protein isolated from seeds of the Abrus pulchellus tenuiflorus plant. This study aims to obtain active and homogeneous protein for structural and biological studies that will clarify the functional aspects of this toxin. The DNA fragment encoding pulchel...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	The FEBS journal 2005-03, Vol.272 (5), p.1201-1210
Hauptverfasser:	Silva, André L C, Goto, Leandro S, Dinarte, Anemari R, Hansen, Daiane, Moreira, Renato A, Beltramini, Leila M, Araújo, Ana P U
Format:	Artikel
Sprache:	eng
Schlagworte:	Abrus - chemistry Abrus - genetics Abrus pulchellus Amino Acid Sequence Amino acids Animals Bacteria Circular Dichroism Cloning, Molecular DNA, Complementary - genetics DNA, Complementary - isolation & purification DNA, Plant - genetics DNA, Plant - metabolism Escherichia coli Injections, Intraperitoneal Mice Molecular Sequence Data N-Glycosyl Hydrolases - metabolism Plant Proteins - chemistry Plant Proteins - metabolism Plant Proteins - toxicity Protein Conformation Protein Subunits - chemistry Protein Subunits - metabolism Protein Subunits - toxicity Proteins Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - metabolism Recombinant Fusion Proteins - toxicity Ribosomes - metabolism RNA, Fungal - genetics RNA, Fungal - metabolism RNA, Plant - genetics RNA, Plant - metabolism RNA, Ribosomal - genetics RNA, Ribosomal - metabolism Saccharomyces cerevisiae - metabolism Seeds - chemistry Sequence Homology, Amino Acid Toxins Yeast
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!