Isolation and physiological characterization of the pentachlorophenol degrading bacterium Sphingomonas chlorophenolica
Many chlorophenols tend to persist in the environment, and they may become public health hazards. Among chlorophenols, pentachlorophenol (PCP) is a priority pollutant that has been used widely as a general biocide in commercial wood treatment. Owing to the rapid industrial growth, serious soil and w...
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| Veröffentlicht in: | Chemosphere (Oxford) 2006-02, Vol.62 (5), p.709-714 |
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| creator | Yang, Chu-Fang Lee, Chi-Mei Wang, Chun-Chin |
| description | Many chlorophenols tend to persist in the environment, and they may become public health hazards. Among chlorophenols, pentachlorophenol (PCP) is a priority pollutant that has been used widely as a general biocide in commercial wood treatment. Owing to the rapid industrial growth, serious soil and water pollutions by chlorophenols has been reported in Taiwan. In this study, 10 indigenous PCP-degrading bacterial strains were isolated from a PCP-degrading mixed culture, and the potential of both the pure and mixed cultures for PCP degradation compared. Moreover, the physiological characteristics and optimum growth conditions of the PCP-degrading bacteria were investigated. One of the isolated bacterial strains with good potential for PCP degradation was characterized and identified as
Sphingomonas chlorophenolica by 16S rDNA gene analysis. The result of the optimum growth temperatures revealed that this organism was a mesophile. The optimum pH for PCP removal by
S. chlorophenolica was between 6.9 and 7.6. Increase in concentration of PCP has a negative effect on the biodegradation potential of
S. chlorophenolica and PCP concentration above 600
mg
l
−1 was inhibitory to its growth. The results of this study indicate that this
S. chlorophenolica strain has a better potential for PCP degradation compared to the enriched mixed culture. The physiological characterization of the isolates also indicates the possible application of this strain for bioremediation of sites contaminated with PCP. |
| doi_str_mv | 10.1016/j.chemosphere.2005.05.012 |
| format | Article |
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Sphingomonas chlorophenolica by 16S rDNA gene analysis. The result of the optimum growth temperatures revealed that this organism was a mesophile. The optimum pH for PCP removal by
S. chlorophenolica was between 6.9 and 7.6. Increase in concentration of PCP has a negative effect on the biodegradation potential of
S. chlorophenolica and PCP concentration above 600
mg
l
−1 was inhibitory to its growth. The results of this study indicate that this
S. chlorophenolica strain has a better potential for PCP degradation compared to the enriched mixed culture. The physiological characterization of the isolates also indicates the possible application of this strain for bioremediation of sites contaminated with PCP.</description><identifier>ISSN: 0045-6535</identifier><identifier>EISSN: 1879-1298</identifier><identifier>DOI: 10.1016/j.chemosphere.2005.05.012</identifier><identifier>PMID: 16005492</identifier><identifier>CODEN: CMSHAF</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Applied sciences ; Biodegradation ; Biodegradation of pollutants ; Biolog ; Biological and medical sciences ; Biological and physicochemical phenomena ; Biotechnology ; Decontamination. Miscellaneous ; DNA, Ribosomal - genetics ; Earth sciences ; Earth, ocean, space ; Engineering and environment geology. Geothermics ; Environment and pollution ; Environmental Pollutants - metabolism ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; genes ; Growth physiology ; Hydrogen-Ion Concentration ; Industrial applications and implications. Economical aspects ; molecular sequence data ; Natural water pollution ; nucleotide sequences ; Pentachlorophenol ; Pentachlorophenol - metabolism ; Pollution ; Pollution, environment geology ; ribosomal DNA ; ribosomal RNA ; Soil and sediments pollution ; Soil Microbiology ; Sphingomonas - isolation & purification ; Sphingomonas - physiology ; Sphingomonas chlorophenolica ; Taiwan ; Temperature ; Water treatment and pollution</subject><ispartof>Chemosphere (Oxford), 2006-02, Vol.62 (5), p.709-714</ispartof><rights>2005 Elsevier Ltd</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c557t-c5ec6687a26c304c97f5644a9f7c879a94ada54e29ec868d466a0fbdc55f4fd53</citedby><cites>FETCH-LOGICAL-c557t-c5ec6687a26c304c97f5644a9f7c879a94ada54e29ec868d466a0fbdc55f4fd53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.chemosphere.2005.05.012$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17509234$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16005492$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Chu-Fang</creatorcontrib><creatorcontrib>Lee, Chi-Mei</creatorcontrib><creatorcontrib>Wang, Chun-Chin</creatorcontrib><title>Isolation and physiological characterization of the pentachlorophenol degrading bacterium Sphingomonas chlorophenolica</title><title>Chemosphere (Oxford)</title><addtitle>Chemosphere</addtitle><description>Many chlorophenols tend to persist in the environment, and they may become public health hazards. Among chlorophenols, pentachlorophenol (PCP) is a priority pollutant that has been used widely as a general biocide in commercial wood treatment. Owing to the rapid industrial growth, serious soil and water pollutions by chlorophenols has been reported in Taiwan. In this study, 10 indigenous PCP-degrading bacterial strains were isolated from a PCP-degrading mixed culture, and the potential of both the pure and mixed cultures for PCP degradation compared. Moreover, the physiological characteristics and optimum growth conditions of the PCP-degrading bacteria were investigated. One of the isolated bacterial strains with good potential for PCP degradation was characterized and identified as
Sphingomonas chlorophenolica by 16S rDNA gene analysis. The result of the optimum growth temperatures revealed that this organism was a mesophile. The optimum pH for PCP removal by
S. chlorophenolica was between 6.9 and 7.6. Increase in concentration of PCP has a negative effect on the biodegradation potential of
S. chlorophenolica and PCP concentration above 600
mg
l
−1 was inhibitory to its growth. The results of this study indicate that this
S. chlorophenolica strain has a better potential for PCP degradation compared to the enriched mixed culture. The physiological characterization of the isolates also indicates the possible application of this strain for bioremediation of sites contaminated with PCP.</description><subject>Applied sciences</subject><subject>Biodegradation</subject><subject>Biodegradation of pollutants</subject><subject>Biolog</subject><subject>Biological and medical sciences</subject><subject>Biological and physicochemical phenomena</subject><subject>Biotechnology</subject><subject>Decontamination. Miscellaneous</subject><subject>DNA, Ribosomal - genetics</subject><subject>Earth sciences</subject><subject>Earth, ocean, space</subject><subject>Engineering and environment geology. Geothermics</subject><subject>Environment and pollution</subject><subject>Environmental Pollutants - metabolism</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genes</subject><subject>Growth physiology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>molecular sequence data</subject><subject>Natural water pollution</subject><subject>nucleotide sequences</subject><subject>Pentachlorophenol</subject><subject>Pentachlorophenol - metabolism</subject><subject>Pollution</subject><subject>Pollution, environment geology</subject><subject>ribosomal DNA</subject><subject>ribosomal RNA</subject><subject>Soil and sediments pollution</subject><subject>Soil Microbiology</subject><subject>Sphingomonas - isolation & purification</subject><subject>Sphingomonas - physiology</subject><subject>Sphingomonas chlorophenolica</subject><subject>Taiwan</subject><subject>Temperature</subject><subject>Water treatment and pollution</subject><issn>0045-6535</issn><issn>1879-1298</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhi0EokvhL0A40NsuduKP-IhWpVSqxKH0bM3a441XSRzsbKXy6_EqK7U3kEa2ZD3PeOyXkM-Mbhhl8uthYzscYp46TLipKRWbU7H6FVmxVuk1q3X7mqwo5WItRSMuyLucD5QWWei35ILJ4nBdr8jjbY49zCGOFYyumrqnHGIf98FCX9kOEtgZU_izINFXc4fVhOMMtutjimWEMfaVw30CF8Z9tVuE41DdT105iEMcIVcv6dL7PXnjoc_44bxfkofv17-2P9Z3P29ut9_u1lYINZcVrZStglrahnKrlReSc9Be2fJO0BwcCI61RtvK1nEpgfqdK7bn3onmklwtfacUfx8xz2YI2WLfw4jxmA1TXDVatP8GuZKNaFgB9QLaFHNO6M2UwgDpyTBqTumYg3mRjjmlY07F6uJ-PF9y3A3ons1zHAX4cgYglwB8gtGG_MwpQQvFC_dp4TxEA_tUmIf7mrKGMtpyrXQhtguB5XcfAyaTbcDRogsJ7WxcDP8x8F_iwcDw</recordid><startdate>20060201</startdate><enddate>20060201</enddate><creator>Yang, Chu-Fang</creator><creator>Lee, Chi-Mei</creator><creator>Wang, Chun-Chin</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TV</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20060201</creationdate><title>Isolation and physiological characterization of the pentachlorophenol degrading bacterium Sphingomonas chlorophenolica</title><author>Yang, Chu-Fang ; Lee, Chi-Mei ; Wang, Chun-Chin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c557t-c5ec6687a26c304c97f5644a9f7c879a94ada54e29ec868d466a0fbdc55f4fd53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Applied sciences</topic><topic>Biodegradation</topic><topic>Biodegradation of pollutants</topic><topic>Biolog</topic><topic>Biological and medical sciences</topic><topic>Biological and physicochemical phenomena</topic><topic>Biotechnology</topic><topic>Decontamination. Miscellaneous</topic><topic>DNA, Ribosomal - genetics</topic><topic>Earth sciences</topic><topic>Earth, ocean, space</topic><topic>Engineering and environment geology. Geothermics</topic><topic>Environment and pollution</topic><topic>Environmental Pollutants - metabolism</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genes</topic><topic>Growth physiology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>molecular sequence data</topic><topic>Natural water pollution</topic><topic>nucleotide sequences</topic><topic>Pentachlorophenol</topic><topic>Pentachlorophenol - metabolism</topic><topic>Pollution</topic><topic>Pollution, environment geology</topic><topic>ribosomal DNA</topic><topic>ribosomal RNA</topic><topic>Soil and sediments pollution</topic><topic>Soil Microbiology</topic><topic>Sphingomonas - isolation & purification</topic><topic>Sphingomonas - physiology</topic><topic>Sphingomonas chlorophenolica</topic><topic>Taiwan</topic><topic>Temperature</topic><topic>Water treatment and pollution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Chu-Fang</creatorcontrib><creatorcontrib>Lee, Chi-Mei</creatorcontrib><creatorcontrib>Wang, Chun-Chin</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Pollution Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Chemosphere (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Chu-Fang</au><au>Lee, Chi-Mei</au><au>Wang, Chun-Chin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and physiological characterization of the pentachlorophenol degrading bacterium Sphingomonas chlorophenolica</atitle><jtitle>Chemosphere (Oxford)</jtitle><addtitle>Chemosphere</addtitle><date>2006-02-01</date><risdate>2006</risdate><volume>62</volume><issue>5</issue><spage>709</spage><epage>714</epage><pages>709-714</pages><issn>0045-6535</issn><eissn>1879-1298</eissn><coden>CMSHAF</coden><abstract>Many chlorophenols tend to persist in the environment, and they may become public health hazards. Among chlorophenols, pentachlorophenol (PCP) is a priority pollutant that has been used widely as a general biocide in commercial wood treatment. Owing to the rapid industrial growth, serious soil and water pollutions by chlorophenols has been reported in Taiwan. In this study, 10 indigenous PCP-degrading bacterial strains were isolated from a PCP-degrading mixed culture, and the potential of both the pure and mixed cultures for PCP degradation compared. Moreover, the physiological characteristics and optimum growth conditions of the PCP-degrading bacteria were investigated. One of the isolated bacterial strains with good potential for PCP degradation was characterized and identified as
Sphingomonas chlorophenolica by 16S rDNA gene analysis. The result of the optimum growth temperatures revealed that this organism was a mesophile. The optimum pH for PCP removal by
S. chlorophenolica was between 6.9 and 7.6. Increase in concentration of PCP has a negative effect on the biodegradation potential of
S. chlorophenolica and PCP concentration above 600
mg
l
−1 was inhibitory to its growth. The results of this study indicate that this
S. chlorophenolica strain has a better potential for PCP degradation compared to the enriched mixed culture. The physiological characterization of the isolates also indicates the possible application of this strain for bioremediation of sites contaminated with PCP.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>16005492</pmid><doi>10.1016/j.chemosphere.2005.05.012</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Chemosphere (Oxford), 2006-02, Vol.62 (5), p.709-714 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Applied sciences Biodegradation Biodegradation of pollutants Biolog Biological and medical sciences Biological and physicochemical phenomena Biotechnology Decontamination. Miscellaneous DNA, Ribosomal - genetics Earth sciences Earth, ocean, space Engineering and environment geology. Geothermics Environment and pollution Environmental Pollutants - metabolism Exact sciences and technology Fundamental and applied biological sciences. Psychology genes Growth physiology Hydrogen-Ion Concentration Industrial applications and implications. Economical aspects molecular sequence data Natural water pollution nucleotide sequences Pentachlorophenol Pentachlorophenol - metabolism Pollution Pollution, environment geology ribosomal DNA ribosomal RNA Soil and sediments pollution Soil Microbiology Sphingomonas - isolation & purification Sphingomonas - physiology Sphingomonas chlorophenolica Taiwan Temperature Water treatment and pollution |
| title | Isolation and physiological characterization of the pentachlorophenol degrading bacterium Sphingomonas chlorophenolica |
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