Electroacupuncture ameliorates cognitive impairment and regulates the expression of apoptosis-related genes Bcl-2 and Bax in rats with cerebral ischaemia-reperfusion injury
BackgroundPost-stroke cognitive impairment seriously affects the quality of life and functional rehabilitation of patients with stroke.ObjectiveTo examine the effects of electroacupuncture (EA) at GV20 and GV24 on cognitive impairment and apoptosis including expression of apoptosis-related genes Bcl...
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| Veröffentlicht in: | Acupuncture in medicine : journal of the British Medical Acupuncture Society 2015-12, Vol.33 (6), p.478-484 |
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| container_title | Acupuncture in medicine : journal of the British Medical Acupuncture Society |
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| creator | Liu, Fang Jiang, Yi-Jing Zhao, Hong-Jia Yao, Li-Qun Chen, Li-Dian |
| description | BackgroundPost-stroke cognitive impairment seriously affects the quality of life and functional rehabilitation of patients with stroke.ObjectiveTo examine the effects of electroacupuncture (EA) at GV20 and GV24 on cognitive impairment and apoptosis including expression of apoptosis-related genes Bcl-2 and Bax in a rat model of cerebral ischaemia-reperfusion (IR) induced by middle cerebral artery occlusion (MCAO).MethodsThirty-five Sprague-Dawley rats were allocated to a sham operation control group (SC group, n=10) or underwent surgery and MCAO (n=25). Postoperatively the latter group was randomly subdivided into EA or untreated (IR) groups. Cognitive impairment was assessed using the Morris water maze (MWM). Apoptosis was examined by detection of Bcl-2 and Bax expression in the cerebral cortex.ResultsThe EA group had significantly decreased neurological deficit scores compared to the IR group (p |
| doi_str_mv | 10.1136/acupmed-2014-010728 |
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Postoperatively the latter group was randomly subdivided into EA or untreated (IR) groups. Cognitive impairment was assessed using the Morris water maze (MWM). Apoptosis was examined by detection of Bcl-2 and Bax expression in the cerebral cortex.ResultsThe EA group had significantly decreased neurological deficit scores compared to the IR group (p<0.05). In the MWM test, significant differences in escape latency and route were observed between the EA and IR groups (p<0.05). Rats in the EA group performed better in the probe trial than those in the IR group (p<0.05). EA treatment markedly reduced the number of TUNEL-positive cells compared to the IR group (20.13±4.30% vs 38.40±3.38%; p<0.001). Reverse transcription-polymerase chain reaction (RT-PCR) results showed the Bcl-2/Bax ratio was significantly increased in the EA group compared to the IR group (1.61±0.19 vs 0.50±0.05, p<0.01).ConclusionsThese findings suggest that EA ameliorates cognitive impairment of rats with IR injury by modulating Bcl-2 and Bax expression.</description><identifier>ISSN: 0964-5284</identifier><identifier>EISSN: 1759-9873</identifier><identifier>DOI: 10.1136/acupmed-2014-010728</identifier><identifier>PMID: 26376847</identifier><language>eng</language><publisher>England: Sage Publications Ltd</publisher><subject>Acupuncture Points ; Animals ; Apoptosis - genetics ; bcl-2-Associated X Protein - genetics ; Brain Ischemia - complications ; Brain Ischemia - therapy ; Cognition Disorders - etiology ; Cognition Disorders - therapy ; Disease Models, Animal ; Electroacupuncture ; Gene Expression Regulation ; Genes, bcl-2 ; Neurons - pathology ; Rats ; Rats, Sprague-Dawley ; Reperfusion Injury - complications ; Reperfusion Injury - therapy</subject><ispartof>Acupuncture in medicine : journal of the British Medical Acupuncture Society, 2015-12, Vol.33 (6), p.478-484</ispartof><rights>Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing</rights><rights>Copyright: 2015 Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b442t-87e553ae594673fe19743e7313bbdf297564cc2938dd1e5a954a19b2991c817b3</citedby><cites>FETCH-LOGICAL-b442t-87e553ae594673fe19743e7313bbdf297564cc2938dd1e5a954a19b2991c817b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26376847$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Fang</creatorcontrib><creatorcontrib>Jiang, Yi-Jing</creatorcontrib><creatorcontrib>Zhao, Hong-Jia</creatorcontrib><creatorcontrib>Yao, Li-Qun</creatorcontrib><creatorcontrib>Chen, Li-Dian</creatorcontrib><title>Electroacupuncture ameliorates cognitive impairment and regulates the expression of apoptosis-related genes Bcl-2 and Bax in rats with cerebral ischaemia-reperfusion injury</title><title>Acupuncture in medicine : journal of the British Medical Acupuncture Society</title><addtitle>Acupunct Med</addtitle><description>BackgroundPost-stroke cognitive impairment seriously affects the quality of life and functional rehabilitation of patients with stroke.ObjectiveTo examine the effects of electroacupuncture (EA) at GV20 and GV24 on cognitive impairment and apoptosis including expression of apoptosis-related genes Bcl-2 and Bax in a rat model of cerebral ischaemia-reperfusion (IR) induced by middle cerebral artery occlusion (MCAO).MethodsThirty-five Sprague-Dawley rats were allocated to a sham operation control group (SC group, n=10) or underwent surgery and MCAO (n=25). Postoperatively the latter group was randomly subdivided into EA or untreated (IR) groups. Cognitive impairment was assessed using the Morris water maze (MWM). Apoptosis was examined by detection of Bcl-2 and Bax expression in the cerebral cortex.ResultsThe EA group had significantly decreased neurological deficit scores compared to the IR group (p<0.05). In the MWM test, significant differences in escape latency and route were observed between the EA and IR groups (p<0.05). Rats in the EA group performed better in the probe trial than those in the IR group (p<0.05). EA treatment markedly reduced the number of TUNEL-positive cells compared to the IR group (20.13±4.30% vs 38.40±3.38%; p<0.001). Reverse transcription-polymerase chain reaction (RT-PCR) results showed the Bcl-2/Bax ratio was significantly increased in the EA group compared to the IR group (1.61±0.19 vs 0.50±0.05, p<0.01).ConclusionsThese findings suggest that EA ameliorates cognitive impairment of rats with IR injury by modulating Bcl-2 and Bax expression.</description><subject>Acupuncture Points</subject><subject>Animals</subject><subject>Apoptosis - genetics</subject><subject>bcl-2-Associated X Protein - genetics</subject><subject>Brain Ischemia - complications</subject><subject>Brain Ischemia - therapy</subject><subject>Cognition Disorders - etiology</subject><subject>Cognition Disorders - therapy</subject><subject>Disease Models, Animal</subject><subject>Electroacupuncture</subject><subject>Gene Expression Regulation</subject><subject>Genes, bcl-2</subject><subject>Neurons - pathology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reperfusion Injury - complications</subject><subject>Reperfusion Injury - therapy</subject><issn>0964-5284</issn><issn>1759-9873</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqNkc1u3CAURlHVqpkmfYJKFVI33TgBAwaWTZT-SJGyadYWxtczjGxwATfJO_Uhi2cmm666YsH5vnt1D0IfKLmklDVXxi7zBH1VE8orQoms1Su0oVLoSivJXqMN0Q2vRK34GXqX0p4QoqTgb9FZ3TDZKC436M_tCDbHsJYt3uYlAjYTjC5EkyFhG7beZfcbsJtm4-IEPmPjexxhu4wHJO8Aw9McISUXPA4DNnOYc0guVRFWpsdb8IW8tmNVH9LX5gk7j8uMhB9d3mELEbpoRuyS3RmYnCnZGeKwHEqd3y_x-QK9GcyY4P3pPUcPX29_3nyv7u6__bj5cld1nNe5UhKEYAaE5o1kA1AtOQPJKOu6fqi1FA23ttZM9T0FYbTghuqu1ppaRWXHztHnY-8cw68FUm6nshaMo_EQltRSySUrNxSsoJ_-Qfdhib5s11JFhFRl3EqxI2VjSCnC0M7RTSY-t5S0q8z2JLNdZbZHmSX18dS9dOvfS-bFXgEuj0A37f-r8S-Lna6v</recordid><startdate>20151201</startdate><enddate>20151201</enddate><creator>Liu, Fang</creator><creator>Jiang, Yi-Jing</creator><creator>Zhao, Hong-Jia</creator><creator>Yao, Li-Qun</creator><creator>Chen, Li-Dian</creator><general>Sage Publications Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>BTHHO</scope><scope>CCPQU</scope><scope>EHMNL</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20151201</creationdate><title>Electroacupuncture ameliorates cognitive impairment and regulates the expression of apoptosis-related genes Bcl-2 and Bax in rats with cerebral ischaemia-reperfusion injury</title><author>Liu, Fang ; Jiang, Yi-Jing ; Zhao, Hong-Jia ; Yao, Li-Qun ; Chen, Li-Dian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b442t-87e553ae594673fe19743e7313bbdf297564cc2938dd1e5a954a19b2991c817b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Acupuncture Points</topic><topic>Animals</topic><topic>Apoptosis - genetics</topic><topic>bcl-2-Associated X Protein - genetics</topic><topic>Brain Ischemia - complications</topic><topic>Brain Ischemia - therapy</topic><topic>Cognition Disorders - etiology</topic><topic>Cognition Disorders - therapy</topic><topic>Disease Models, Animal</topic><topic>Electroacupuncture</topic><topic>Gene Expression Regulation</topic><topic>Genes, bcl-2</topic><topic>Neurons - pathology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reperfusion Injury - complications</topic><topic>Reperfusion Injury - therapy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Fang</creatorcontrib><creatorcontrib>Jiang, Yi-Jing</creatorcontrib><creatorcontrib>Zhao, Hong-Jia</creatorcontrib><creatorcontrib>Yao, Li-Qun</creatorcontrib><creatorcontrib>Chen, Li-Dian</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Nursing and Allied Health Journals</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>British Nursing Database</collection><collection>ProQuest Central</collection><collection>BMJ Journals</collection><collection>ProQuest One Community College</collection><collection>UK & Ireland Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Acupuncture in medicine : journal of the British Medical Acupuncture Society</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Fang</au><au>Jiang, Yi-Jing</au><au>Zhao, Hong-Jia</au><au>Yao, Li-Qun</au><au>Chen, Li-Dian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electroacupuncture ameliorates cognitive impairment and regulates the expression of apoptosis-related genes Bcl-2 and Bax in rats with cerebral ischaemia-reperfusion injury</atitle><jtitle>Acupuncture in medicine : journal of the British Medical Acupuncture Society</jtitle><addtitle>Acupunct Med</addtitle><date>2015-12-01</date><risdate>2015</risdate><volume>33</volume><issue>6</issue><spage>478</spage><epage>484</epage><pages>478-484</pages><issn>0964-5284</issn><eissn>1759-9873</eissn><abstract>BackgroundPost-stroke cognitive impairment seriously affects the quality of life and functional rehabilitation of patients with stroke.ObjectiveTo examine the effects of electroacupuncture (EA) at GV20 and GV24 on cognitive impairment and apoptosis including expression of apoptosis-related genes Bcl-2 and Bax in a rat model of cerebral ischaemia-reperfusion (IR) induced by middle cerebral artery occlusion (MCAO).MethodsThirty-five Sprague-Dawley rats were allocated to a sham operation control group (SC group, n=10) or underwent surgery and MCAO (n=25). Postoperatively the latter group was randomly subdivided into EA or untreated (IR) groups. Cognitive impairment was assessed using the Morris water maze (MWM). Apoptosis was examined by detection of Bcl-2 and Bax expression in the cerebral cortex.ResultsThe EA group had significantly decreased neurological deficit scores compared to the IR group (p<0.05). In the MWM test, significant differences in escape latency and route were observed between the EA and IR groups (p<0.05). Rats in the EA group performed better in the probe trial than those in the IR group (p<0.05). EA treatment markedly reduced the number of TUNEL-positive cells compared to the IR group (20.13±4.30% vs 38.40±3.38%; p<0.001). Reverse transcription-polymerase chain reaction (RT-PCR) results showed the Bcl-2/Bax ratio was significantly increased in the EA group compared to the IR group (1.61±0.19 vs 0.50±0.05, p<0.01).ConclusionsThese findings suggest that EA ameliorates cognitive impairment of rats with IR injury by modulating Bcl-2 and Bax expression.</abstract><cop>England</cop><pub>Sage Publications Ltd</pub><pmid>26376847</pmid><doi>10.1136/acupmed-2014-010728</doi><tpages>7</tpages></addata></record> |
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| ispartof | Acupuncture in medicine : journal of the British Medical Acupuncture Society, 2015-12, Vol.33 (6), p.478-484 |
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| subjects | Acupuncture Points Animals Apoptosis - genetics bcl-2-Associated X Protein - genetics Brain Ischemia - complications Brain Ischemia - therapy Cognition Disorders - etiology Cognition Disorders - therapy Disease Models, Animal Electroacupuncture Gene Expression Regulation Genes, bcl-2 Neurons - pathology Rats Rats, Sprague-Dawley Reperfusion Injury - complications Reperfusion Injury - therapy |
| title | Electroacupuncture ameliorates cognitive impairment and regulates the expression of apoptosis-related genes Bcl-2 and Bax in rats with cerebral ischaemia-reperfusion injury |
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