Clinical Evaluation of a Lead Mobilization Test Using the Chelating Agent Dimercaptosuccinic Acid
The lead mobilization test reflects the mobilizable and likely toxicologically active fraction of the lead body burden. We propose a safe and convenient protocol for this test, to assess concomitant copper and zinc excretion and to determine the size of the chelatable lead pool in nonoccupationally...
Gespeichert in:
| Veröffentlicht in: | Clinical chemistry (Baltimore, Md.) Md.), 2006-01, Vol.52 (1), p.88-96 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 96 |
|---|---|
| container_issue | 1 |
| container_start_page | 88 |
| container_title | Clinical chemistry (Baltimore, Md.) |
| container_volume | 52 |
| creator | Hoet, Perrine Buchet, Jean-Pierre Decerf, Laurence Lavalleye, Benoit Haufroid, Vincent Lison, Dominique |
| description | The lead mobilization test reflects the mobilizable and likely toxicologically active fraction of the lead body burden. We propose a safe and convenient protocol for this test, to assess concomitant copper and zinc excretion and to determine the size of the chelatable lead pool in nonoccupationally exposed adults.
The study population included 80 white adults: 40 controls [median blood lead concentration (PbB), 25 microg/L] and 40 lead-exposed individuals (315 microg/L). After collection of 4- and 24-h baseline urine specimens and a blood sample, dimercaptosuccinic acid (DMSA) was administered orally (1 g), and additional 4- and 24-h urine specimens were obtained. Determinants of the chelatable urinary lead (DMSA-PbU) were traced by linear regression analysis.
Urinary DMSA and lead excretion peaked within 2-3 h after DMSA administration. The amounts of DMSA, lead, copper, and zinc recovered in the 4-h urinary collections were highly correlated with those in 24-h collections (r = 0.857, 0.859, 0.958, and 0.757, respectively). At PbB concentrations >300 microg/L, the relationship between DMSA-PbU and PbB showed a steep increase and a widespread dispersion of DMSA-PbU around the regression line. After DMSA, copper and zinc excretion rates were increased up to 91- and 33-fold, respectively. No side effects were reported after DMSA.
Determination of DMSA-PbU in a 4-h collection after DMSA is convenient, apparently safe, and inexpensive. An upper reference limit value of 22 microg/4 h is proposed for Belgian reference individuals. The diagnostic value of DMSA-PbU is likely to be contributive for PbB >300 microg/L. |
| doi_str_mv | 10.1373/clinchem.2005.051128 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_17471438</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>958377861</sourcerecordid><originalsourceid>FETCH-LOGICAL-c468t-e0af21bb666cf3af207cfc15c159ade9c6d9fd9ac00ab03950904e9af734776d3</originalsourceid><addsrcrecordid>eNpdkNFq2zAUhsVYWdNubzCGGOvunEmWLFmXIevWQspu2mtxLEuximxnkr2wPf0UnFEYCKRz9J2fw4fQe0rWlEn2xQQ_mM7265KQak0qSsv6FVrRipGirgR9jVaEEFUoyuUlukrpOZdc1uINuqSiZIpxskKwzTHeQMC3vyDMMPlxwKPDgHcWWvwwNj74P0v70aYJPyU_7PHUWbztbMgfudrs7TDhr7630cBhGtNszCkVb4xv36ILByHZd-f7Gj19u33c3hW7H9_vt5tdYbiop8IScCVtGiGEcSy_iTTO0CofBa1VRrTKtQoMIdAQpiqiCLcKnGRcStGya_R5yT3E8eecV9W9T8aGAIMd56Sp5JJyVmfw43_g8zjHIe-mS8pPziTJEF8gE8eUonX6EH0P8bemRJ_863_-9cm_XvznsQ_n7LnpbfsydBaegZszAClbdxEG49MLJ7kqhawy92nhOr_vjj5anXoIIcdSfTweq1JTXdfsL1-DnPE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>214000970</pqid></control><display><type>article</type><title>Clinical Evaluation of a Lead Mobilization Test Using the Chelating Agent Dimercaptosuccinic Acid</title><source>MEDLINE</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Hoet, Perrine ; Buchet, Jean-Pierre ; Decerf, Laurence ; Lavalleye, Benoit ; Haufroid, Vincent ; Lison, Dominique</creator><creatorcontrib>Hoet, Perrine ; Buchet, Jean-Pierre ; Decerf, Laurence ; Lavalleye, Benoit ; Haufroid, Vincent ; Lison, Dominique</creatorcontrib><description>The lead mobilization test reflects the mobilizable and likely toxicologically active fraction of the lead body burden. We propose a safe and convenient protocol for this test, to assess concomitant copper and zinc excretion and to determine the size of the chelatable lead pool in nonoccupationally exposed adults.
The study population included 80 white adults: 40 controls [median blood lead concentration (PbB), 25 microg/L] and 40 lead-exposed individuals (315 microg/L). After collection of 4- and 24-h baseline urine specimens and a blood sample, dimercaptosuccinic acid (DMSA) was administered orally (1 g), and additional 4- and 24-h urine specimens were obtained. Determinants of the chelatable urinary lead (DMSA-PbU) were traced by linear regression analysis.
Urinary DMSA and lead excretion peaked within 2-3 h after DMSA administration. The amounts of DMSA, lead, copper, and zinc recovered in the 4-h urinary collections were highly correlated with those in 24-h collections (r = 0.857, 0.859, 0.958, and 0.757, respectively). At PbB concentrations >300 microg/L, the relationship between DMSA-PbU and PbB showed a steep increase and a widespread dispersion of DMSA-PbU around the regression line. After DMSA, copper and zinc excretion rates were increased up to 91- and 33-fold, respectively. No side effects were reported after DMSA.
Determination of DMSA-PbU in a 4-h collection after DMSA is convenient, apparently safe, and inexpensive. An upper reference limit value of 22 microg/4 h is proposed for Belgian reference individuals. The diagnostic value of DMSA-PbU is likely to be contributive for PbB >300 microg/L.</description><identifier>ISSN: 0009-9147</identifier><identifier>EISSN: 1530-8561</identifier><identifier>DOI: 10.1373/clinchem.2005.051128</identifier><identifier>PMID: 16239340</identifier><identifier>CODEN: CLCHAU</identifier><language>eng</language><publisher>Washington, DC: Am Assoc Clin Chem</publisher><subject>Adult ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Blood levels ; Body burden ; Chelating agents ; Chelating Agents - pharmacokinetics ; Copper ; Copper - urine ; Environmental Pollutants - urine ; Excretion ; Female ; Fundamental and applied biological sciences. Psychology ; Humans ; Investigative techniques, diagnostic techniques (general aspects) ; Lead - urine ; Lead Poisoning - urine ; Male ; Medical sciences ; Middle Aged ; Occupational Diseases - urine ; Regression analysis ; Succimer - pharmacokinetics ; Zinc ; Zinc - urine</subject><ispartof>Clinical chemistry (Baltimore, Md.), 2006-01, Vol.52 (1), p.88-96</ispartof><rights>2006 INIST-CNRS</rights><rights>Copyright American Association for Clinical Chemistry Jan 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c468t-e0af21bb666cf3af207cfc15c159ade9c6d9fd9ac00ab03950904e9af734776d3</citedby><cites>FETCH-LOGICAL-c468t-e0af21bb666cf3af207cfc15c159ade9c6d9fd9ac00ab03950904e9af734776d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17492675$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16239340$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hoet, Perrine</creatorcontrib><creatorcontrib>Buchet, Jean-Pierre</creatorcontrib><creatorcontrib>Decerf, Laurence</creatorcontrib><creatorcontrib>Lavalleye, Benoit</creatorcontrib><creatorcontrib>Haufroid, Vincent</creatorcontrib><creatorcontrib>Lison, Dominique</creatorcontrib><title>Clinical Evaluation of a Lead Mobilization Test Using the Chelating Agent Dimercaptosuccinic Acid</title><title>Clinical chemistry (Baltimore, Md.)</title><addtitle>Clin Chem</addtitle><description>The lead mobilization test reflects the mobilizable and likely toxicologically active fraction of the lead body burden. We propose a safe and convenient protocol for this test, to assess concomitant copper and zinc excretion and to determine the size of the chelatable lead pool in nonoccupationally exposed adults.
The study population included 80 white adults: 40 controls [median blood lead concentration (PbB), 25 microg/L] and 40 lead-exposed individuals (315 microg/L). After collection of 4- and 24-h baseline urine specimens and a blood sample, dimercaptosuccinic acid (DMSA) was administered orally (1 g), and additional 4- and 24-h urine specimens were obtained. Determinants of the chelatable urinary lead (DMSA-PbU) were traced by linear regression analysis.
Urinary DMSA and lead excretion peaked within 2-3 h after DMSA administration. The amounts of DMSA, lead, copper, and zinc recovered in the 4-h urinary collections were highly correlated with those in 24-h collections (r = 0.857, 0.859, 0.958, and 0.757, respectively). At PbB concentrations >300 microg/L, the relationship between DMSA-PbU and PbB showed a steep increase and a widespread dispersion of DMSA-PbU around the regression line. After DMSA, copper and zinc excretion rates were increased up to 91- and 33-fold, respectively. No side effects were reported after DMSA.
Determination of DMSA-PbU in a 4-h collection after DMSA is convenient, apparently safe, and inexpensive. An upper reference limit value of 22 microg/4 h is proposed for Belgian reference individuals. The diagnostic value of DMSA-PbU is likely to be contributive for PbB >300 microg/L.</description><subject>Adult</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Blood levels</subject><subject>Body burden</subject><subject>Chelating agents</subject><subject>Chelating Agents - pharmacokinetics</subject><subject>Copper</subject><subject>Copper - urine</subject><subject>Environmental Pollutants - urine</subject><subject>Excretion</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Lead - urine</subject><subject>Lead Poisoning - urine</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Occupational Diseases - urine</subject><subject>Regression analysis</subject><subject>Succimer - pharmacokinetics</subject><subject>Zinc</subject><subject>Zinc - urine</subject><issn>0009-9147</issn><issn>1530-8561</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkNFq2zAUhsVYWdNubzCGGOvunEmWLFmXIevWQspu2mtxLEuximxnkr2wPf0UnFEYCKRz9J2fw4fQe0rWlEn2xQQ_mM7265KQak0qSsv6FVrRipGirgR9jVaEEFUoyuUlukrpOZdc1uINuqSiZIpxskKwzTHeQMC3vyDMMPlxwKPDgHcWWvwwNj74P0v70aYJPyU_7PHUWbztbMgfudrs7TDhr7630cBhGtNszCkVb4xv36ILByHZd-f7Gj19u33c3hW7H9_vt5tdYbiop8IScCVtGiGEcSy_iTTO0CofBa1VRrTKtQoMIdAQpiqiCLcKnGRcStGya_R5yT3E8eecV9W9T8aGAIMd56Sp5JJyVmfw43_g8zjHIe-mS8pPziTJEF8gE8eUonX6EH0P8bemRJ_863_-9cm_XvznsQ_n7LnpbfsydBaegZszAClbdxEG49MLJ7kqhawy92nhOr_vjj5anXoIIcdSfTweq1JTXdfsL1-DnPE</recordid><startdate>20060101</startdate><enddate>20060101</enddate><creator>Hoet, Perrine</creator><creator>Buchet, Jean-Pierre</creator><creator>Decerf, Laurence</creator><creator>Lavalleye, Benoit</creator><creator>Haufroid, Vincent</creator><creator>Lison, Dominique</creator><general>Am Assoc Clin Chem</general><general>American Association for Clinical Chemistry</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4U-</scope><scope>7QO</scope><scope>7RV</scope><scope>7TM</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PCBAR</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>S0X</scope></search><sort><creationdate>20060101</creationdate><title>Clinical Evaluation of a Lead Mobilization Test Using the Chelating Agent Dimercaptosuccinic Acid</title><author>Hoet, Perrine ; Buchet, Jean-Pierre ; Decerf, Laurence ; Lavalleye, Benoit ; Haufroid, Vincent ; Lison, Dominique</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c468t-e0af21bb666cf3af207cfc15c159ade9c6d9fd9ac00ab03950904e9af734776d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adult</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Blood levels</topic><topic>Body burden</topic><topic>Chelating agents</topic><topic>Chelating Agents - pharmacokinetics</topic><topic>Copper</topic><topic>Copper - urine</topic><topic>Environmental Pollutants - urine</topic><topic>Excretion</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Lead - urine</topic><topic>Lead Poisoning - urine</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Occupational Diseases - urine</topic><topic>Regression analysis</topic><topic>Succimer - pharmacokinetics</topic><topic>Zinc</topic><topic>Zinc - urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hoet, Perrine</creatorcontrib><creatorcontrib>Buchet, Jean-Pierre</creatorcontrib><creatorcontrib>Decerf, Laurence</creatorcontrib><creatorcontrib>Lavalleye, Benoit</creatorcontrib><creatorcontrib>Haufroid, Vincent</creatorcontrib><creatorcontrib>Lison, Dominique</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>University Readers</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>SIRS Editorial</collection><jtitle>Clinical chemistry (Baltimore, Md.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hoet, Perrine</au><au>Buchet, Jean-Pierre</au><au>Decerf, Laurence</au><au>Lavalleye, Benoit</au><au>Haufroid, Vincent</au><au>Lison, Dominique</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clinical Evaluation of a Lead Mobilization Test Using the Chelating Agent Dimercaptosuccinic Acid</atitle><jtitle>Clinical chemistry (Baltimore, Md.)</jtitle><addtitle>Clin Chem</addtitle><date>2006-01-01</date><risdate>2006</risdate><volume>52</volume><issue>1</issue><spage>88</spage><epage>96</epage><pages>88-96</pages><issn>0009-9147</issn><eissn>1530-8561</eissn><coden>CLCHAU</coden><abstract>The lead mobilization test reflects the mobilizable and likely toxicologically active fraction of the lead body burden. We propose a safe and convenient protocol for this test, to assess concomitant copper and zinc excretion and to determine the size of the chelatable lead pool in nonoccupationally exposed adults.
The study population included 80 white adults: 40 controls [median blood lead concentration (PbB), 25 microg/L] and 40 lead-exposed individuals (315 microg/L). After collection of 4- and 24-h baseline urine specimens and a blood sample, dimercaptosuccinic acid (DMSA) was administered orally (1 g), and additional 4- and 24-h urine specimens were obtained. Determinants of the chelatable urinary lead (DMSA-PbU) were traced by linear regression analysis.
Urinary DMSA and lead excretion peaked within 2-3 h after DMSA administration. The amounts of DMSA, lead, copper, and zinc recovered in the 4-h urinary collections were highly correlated with those in 24-h collections (r = 0.857, 0.859, 0.958, and 0.757, respectively). At PbB concentrations >300 microg/L, the relationship between DMSA-PbU and PbB showed a steep increase and a widespread dispersion of DMSA-PbU around the regression line. After DMSA, copper and zinc excretion rates were increased up to 91- and 33-fold, respectively. No side effects were reported after DMSA.
Determination of DMSA-PbU in a 4-h collection after DMSA is convenient, apparently safe, and inexpensive. An upper reference limit value of 22 microg/4 h is proposed for Belgian reference individuals. The diagnostic value of DMSA-PbU is likely to be contributive for PbB >300 microg/L.</abstract><cop>Washington, DC</cop><pub>Am Assoc Clin Chem</pub><pmid>16239340</pmid><doi>10.1373/clinchem.2005.051128</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0009-9147 |
| ispartof | Clinical chemistry (Baltimore, Md.), 2006-01, Vol.52 (1), p.88-96 |
| issn | 0009-9147 1530-8561 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17471438 |
| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Adult Analytical, structural and metabolic biochemistry Biological and medical sciences Blood levels Body burden Chelating agents Chelating Agents - pharmacokinetics Copper Copper - urine Environmental Pollutants - urine Excretion Female Fundamental and applied biological sciences. Psychology Humans Investigative techniques, diagnostic techniques (general aspects) Lead - urine Lead Poisoning - urine Male Medical sciences Middle Aged Occupational Diseases - urine Regression analysis Succimer - pharmacokinetics Zinc Zinc - urine |
| title | Clinical Evaluation of a Lead Mobilization Test Using the Chelating Agent Dimercaptosuccinic Acid |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T12%3A23%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Clinical%20Evaluation%20of%20a%20Lead%20Mobilization%20Test%20Using%20the%20Chelating%20Agent%20Dimercaptosuccinic%20Acid&rft.jtitle=Clinical%20chemistry%20(Baltimore,%20Md.)&rft.au=Hoet,%20Perrine&rft.date=2006-01-01&rft.volume=52&rft.issue=1&rft.spage=88&rft.epage=96&rft.pages=88-96&rft.issn=0009-9147&rft.eissn=1530-8561&rft.coden=CLCHAU&rft_id=info:doi/10.1373/clinchem.2005.051128&rft_dat=%3Cproquest_cross%3E958377861%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=214000970&rft_id=info:pmid/16239340&rfr_iscdi=true |