Silencing of DGAT1 in tobacco causes a reduction in seed oil content
The 679-bp fragment corresponding to nt 615–1293 of tobacco DGAT1 (GenBank, AF129003) was used to create intron-containing construct expressing hpRNA for silencing endogenous DGAT1 gene of Nicotiana tabacum. The oil content in mature seeds of transgenic lines was reduced by 9 to 49%. The transgenic...
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| Veröffentlicht in: | Plant science (Limerick) 2005-10, Vol.169 (4), p.689-694 |
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| container_title | Plant science (Limerick) |
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| creator | Zhang, Fa-Yun Yang, Ming-Feng Xu, Yi-Nong |
| description | The 679-bp fragment corresponding to nt 615–1293 of tobacco
DGAT1 (GenBank,
AF129003) was used to create intron-containing construct expressing hpRNA for silencing endogenous
DGAT1 gene of
Nicotiana tabacum. The oil content in mature seeds of transgenic lines was reduced by 9 to 49%. The transgenic tobacco, designated
Sil7, is correlated with reduced triacylglycerol (TAG) contents in different tissues, including leaves, stems, roots, petals and mature seeds. We show that the reduction of
DGAT1 transcript level in transgenic tobacco mediated by hpRNA is correlated with the decrease of oil content in different tissues. In company with the decrease of average seed weight, seed oil content reduced, whereas protein and sugar content increased in the seeds of transgenic lines. There is a reversed relation between the deposition of oil and the synthesis of protein and sugar in the seeds of transgenic lines. |
| doi_str_mv | 10.1016/j.plantsci.2005.05.019 |
| format | Article |
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DGAT1 (GenBank,
AF129003) was used to create intron-containing construct expressing hpRNA for silencing endogenous
DGAT1 gene of
Nicotiana tabacum. The oil content in mature seeds of transgenic lines was reduced by 9 to 49%. The transgenic tobacco, designated
Sil7, is correlated with reduced triacylglycerol (TAG) contents in different tissues, including leaves, stems, roots, petals and mature seeds. We show that the reduction of
DGAT1 transcript level in transgenic tobacco mediated by hpRNA is correlated with the decrease of oil content in different tissues. In company with the decrease of average seed weight, seed oil content reduced, whereas protein and sugar content increased in the seeds of transgenic lines. There is a reversed relation between the deposition of oil and the synthesis of protein and sugar in the seeds of transgenic lines.</description><identifier>ISSN: 0168-9452</identifier><identifier>EISSN: 1873-2259</identifier><identifier>DOI: 10.1016/j.plantsci.2005.05.019</identifier><identifier>CODEN: PLSCE4</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>acyltransferases ; biochemical pathways ; Biological and medical sciences ; biosynthesis ; carbohydrate content ; carbohydrate metabolism ; diacylglycerol acyltransferase ; Diacylglycerol acyltransferase (DGAT) ; flowers ; Fundamental and applied biological sciences. Psychology ; gene expression regulation ; Gene silencing ; Germination and dormancy ; lipid content ; Nicotiana tabacum ; nucleotide sequences ; Plant physiology and development ; plant proteins ; protein content ; seed oils ; seeds ; sugars ; Tobacco ; transgenic plants ; Triacylglycerol (TAG) ; triacylglycerols</subject><ispartof>Plant science (Limerick), 2005-10, Vol.169 (4), p.689-694</ispartof><rights>2005 Elsevier Ireland Ltd</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c397t-a94943a524333d261c203a42ab44d5531f4e9507a7a2338fc05dd07d9104e2113</citedby><cites>FETCH-LOGICAL-c397t-a94943a524333d261c203a42ab44d5531f4e9507a7a2338fc05dd07d9104e2113</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.plantsci.2005.05.019$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17016341$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Fa-Yun</creatorcontrib><creatorcontrib>Yang, Ming-Feng</creatorcontrib><creatorcontrib>Xu, Yi-Nong</creatorcontrib><title>Silencing of DGAT1 in tobacco causes a reduction in seed oil content</title><title>Plant science (Limerick)</title><description>The 679-bp fragment corresponding to nt 615–1293 of tobacco
DGAT1 (GenBank,
AF129003) was used to create intron-containing construct expressing hpRNA for silencing endogenous
DGAT1 gene of
Nicotiana tabacum. The oil content in mature seeds of transgenic lines was reduced by 9 to 49%. The transgenic tobacco, designated
Sil7, is correlated with reduced triacylglycerol (TAG) contents in different tissues, including leaves, stems, roots, petals and mature seeds. We show that the reduction of
DGAT1 transcript level in transgenic tobacco mediated by hpRNA is correlated with the decrease of oil content in different tissues. In company with the decrease of average seed weight, seed oil content reduced, whereas protein and sugar content increased in the seeds of transgenic lines. There is a reversed relation between the deposition of oil and the synthesis of protein and sugar in the seeds of transgenic lines.</description><subject>acyltransferases</subject><subject>biochemical pathways</subject><subject>Biological and medical sciences</subject><subject>biosynthesis</subject><subject>carbohydrate content</subject><subject>carbohydrate metabolism</subject><subject>diacylglycerol acyltransferase</subject><subject>Diacylglycerol acyltransferase (DGAT)</subject><subject>flowers</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression regulation</subject><subject>Gene silencing</subject><subject>Germination and dormancy</subject><subject>lipid content</subject><subject>Nicotiana tabacum</subject><subject>nucleotide sequences</subject><subject>Plant physiology and development</subject><subject>plant proteins</subject><subject>protein content</subject><subject>seed oils</subject><subject>seeds</subject><subject>sugars</subject><subject>Tobacco</subject><subject>transgenic plants</subject><subject>Triacylglycerol (TAG)</subject><subject>triacylglycerols</subject><issn>0168-9452</issn><issn>1873-2259</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqFkE1LXDEUhkNpwVH7F2w2urvjyddksqtoq4LgQl2HY3KuZLjeTJM7gv_eXMbSZeFAFnnevCcPYycClgLE6nyz3A44TjWkpQQwy3mE-8IWYm1VJ6VxX9migevOaSMP2GGtGwCQxtgFu3pIA40hjS889_zq-uJR8DTyKT9jCJkH3FWqHHmhuAtTyuN8W4kiz2ngIY8TjdMx-9bjUOn753nEnn7_ery86e7ur28vL-66oJydOnTaaYVGaqVUlCsRJCjUEp-1jsYo0WtyBixalEqt-wAmRrDRCdAkhVBH7Gz_7rbkPzuqk39NNdDQvk95V72w2oKDGVztwVByrYV6vy3pFcu7F-BnaX7j_0rzszQ_j3AtePrZgDXg0Bdsbuq_tG1ZpeeCH3uux-zxpTTm6UG2ZhDQdl-pRvzcE9SEvCUqvnU10xRToTD5mNP_lvkAFRqMlw</recordid><startdate>20051001</startdate><enddate>20051001</enddate><creator>Zhang, Fa-Yun</creator><creator>Yang, Ming-Feng</creator><creator>Xu, Yi-Nong</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20051001</creationdate><title>Silencing of DGAT1 in tobacco causes a reduction in seed oil content</title><author>Zhang, Fa-Yun ; Yang, Ming-Feng ; Xu, Yi-Nong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c397t-a94943a524333d261c203a42ab44d5531f4e9507a7a2338fc05dd07d9104e2113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>acyltransferases</topic><topic>biochemical pathways</topic><topic>Biological and medical sciences</topic><topic>biosynthesis</topic><topic>carbohydrate content</topic><topic>carbohydrate metabolism</topic><topic>diacylglycerol acyltransferase</topic><topic>Diacylglycerol acyltransferase (DGAT)</topic><topic>flowers</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression regulation</topic><topic>Gene silencing</topic><topic>Germination and dormancy</topic><topic>lipid content</topic><topic>Nicotiana tabacum</topic><topic>nucleotide sequences</topic><topic>Plant physiology and development</topic><topic>plant proteins</topic><topic>protein content</topic><topic>seed oils</topic><topic>seeds</topic><topic>sugars</topic><topic>Tobacco</topic><topic>transgenic plants</topic><topic>Triacylglycerol (TAG)</topic><topic>triacylglycerols</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Fa-Yun</creatorcontrib><creatorcontrib>Yang, Ming-Feng</creatorcontrib><creatorcontrib>Xu, Yi-Nong</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Plant science (Limerick)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Fa-Yun</au><au>Yang, Ming-Feng</au><au>Xu, Yi-Nong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Silencing of DGAT1 in tobacco causes a reduction in seed oil content</atitle><jtitle>Plant science (Limerick)</jtitle><date>2005-10-01</date><risdate>2005</risdate><volume>169</volume><issue>4</issue><spage>689</spage><epage>694</epage><pages>689-694</pages><issn>0168-9452</issn><eissn>1873-2259</eissn><coden>PLSCE4</coden><abstract>The 679-bp fragment corresponding to nt 615–1293 of tobacco
DGAT1 (GenBank,
AF129003) was used to create intron-containing construct expressing hpRNA for silencing endogenous
DGAT1 gene of
Nicotiana tabacum. The oil content in mature seeds of transgenic lines was reduced by 9 to 49%. The transgenic tobacco, designated
Sil7, is correlated with reduced triacylglycerol (TAG) contents in different tissues, including leaves, stems, roots, petals and mature seeds. We show that the reduction of
DGAT1 transcript level in transgenic tobacco mediated by hpRNA is correlated with the decrease of oil content in different tissues. In company with the decrease of average seed weight, seed oil content reduced, whereas protein and sugar content increased in the seeds of transgenic lines. There is a reversed relation between the deposition of oil and the synthesis of protein and sugar in the seeds of transgenic lines.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><doi>10.1016/j.plantsci.2005.05.019</doi><tpages>6</tpages></addata></record> |
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| ispartof | Plant science (Limerick), 2005-10, Vol.169 (4), p.689-694 |
| issn | 0168-9452 1873-2259 |
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| source | Access via ScienceDirect (Elsevier) |
| subjects | acyltransferases biochemical pathways Biological and medical sciences biosynthesis carbohydrate content carbohydrate metabolism diacylglycerol acyltransferase Diacylglycerol acyltransferase (DGAT) flowers Fundamental and applied biological sciences. Psychology gene expression regulation Gene silencing Germination and dormancy lipid content Nicotiana tabacum nucleotide sequences Plant physiology and development plant proteins protein content seed oils seeds sugars Tobacco transgenic plants Triacylglycerol (TAG) triacylglycerols |
| title | Silencing of DGAT1 in tobacco causes a reduction in seed oil content |
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