Some special features of glyoxyl supports to immobilize proteins

This paper described that glyoxyl activated supports are only able to quantitatively immobilize proteins at alkaline pH values and using highly activated supports (otherwise, immobilization is negligible). Furthermore, the immobilization rate depends on the surface density of glyoxyl groups and not...

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Veröffentlicht in:	Enzyme and microbial technology 2005-09, Vol.37 (4), p.456-462
Hauptverfasser:	Mateo, Cesar, Abian, Olga, Bernedo, Marieta, Cuenca, Emma, Fuentes, Manuel, Fernandez-Lorente, Gloria, Palomo, Jose M., Grazu, Valeria, Pessela, Benevides C.C., Giacomini, Cecilia, Irazoqui, Gabriela, Villarino, Andrea, Ovsejevi, Karen, Batista-Viera, Francisco, Fernandez-Lafuente, Roberto, Guisán, Jose M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Cyanogen bromide supports Fundamental and applied biological sciences. Psychology Glutaraldehyde supports Glyoxyl supports Immobilization of enzymes and other molecules Immobilization of proteins Immobilization techniques Methods. Procedures. Technologies Orientation of immobilized proteins
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